Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Transcription factor AS1

Gene

AS1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Transcription factor required for normal cell differentiation. Positively regulates LATERAL ORGAN BOUNDARIES (LOB) within the shoot apex, and the class III HD-ZIP genes REV, PHB, and PHV. Interacts directly with ASYMMETRIC LEAVES 2 (LBD6/AS2) to repress the knox homeobox genes BP/KNAT1, KNAT2, and KNAT6 and the abaxial determinants ARF3/ETT, KAN2 and YAB5. May act in parallel with the RDR6-SGS3-AGO7 pathway, an endogenous RNA silencing pathway, to regulate the leaf morphogenesis (PubMed:11076771, PubMed:11140682, PubMed:11882937, PubMed:12750468, PubMed:16006579, PubMed:16699177, PubMed:17395603, PubMed:17559509, PubMed:23271976). Binds directly to KNAT1, KNAT2, and KNATM chromatin, regulating leaf development (PubMed:23271976). LBD6 is required for this binding (PubMed:23271976). Positive regulator of flowering that binds to the promoter of FT (PubMed:21950734). Regulates FT expression by forming a functional complex with CO (PubMed:21950734). Involved in leaf polarity establishment by functioning cooperatively with NUCL1 to repress abaxial genes ARF3, ARF4, KAN1, KAN2, YAB1 and YAB5, and the knox homeobox genes KNAT1, KNAT2, KNAT6, and STM to promote adaxial development in leaf primordia at shoot apical meristems at high temperatures (PubMed:27334696).10 Publications

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi27 – 53H-T-H motifPROSITE-ProRule annotationAdd BLAST27
DNA bindingi81 – 104H-T-H motifPROSITE-ProRule annotationAdd BLAST24

GO - Molecular functioni

  • DNA binding Source: TAIR
  • DNA binding transcription factor activity Source: TAIR
  • protein homodimerization activity Source: TAIR
  • RNA polymerase II transcription factor activity, sequence-specific DNA binding Source: GO_Central
  • sequence-specific DNA binding Source: TAIR
  • transcription factor activity, RNA polymerase II transcription factor recruiting Source: GO_Central
  • transcription regulatory region DNA binding Source: GO_Central

GO - Biological processi

  • asymmetric cell division Source: TAIR
  • cell differentiation Source: GO_Central
  • defense response to bacterium Source: TAIR
  • defense response to fungus Source: TAIR
  • flower development Source: UniProtKB-KW
  • leaf formation Source: TAIR
  • leaf morphogenesis Source: TAIR
  • negative regulation of transcription, DNA-templated Source: TAIR
  • polarity specification of adaxial/abaxial axis Source: TAIR
  • regulation of innate immune response Source: TAIR
  • regulation of transcription by RNA polymerase II Source: GO_Central
  • response to auxin Source: TAIR
  • response to cadmium ion Source: TAIR
  • response to gibberellin Source: TAIR
  • response to jasmonic acid Source: TAIR
  • response to salicylic acid Source: TAIR
  • response to salt stress Source: TAIR
  • response to virus Source: TAIR
  • transcription, DNA-templated Source: UniProtKB-KW

Keywordsi

Molecular functionDNA-binding, Repressor
Biological processFlowering, Stress response, Transcription, Transcription regulation

Names & Taxonomyi

Protein namesi
Recommended name:
Transcription factor AS11 Publication
Alternative name(s):
Myb-related protein 911 Publication
Short name:
AtMYB911 Publication
Protein ASYMMETRIC LEAVES 11 Publication
Protein PHANTASTICA1 Publication
Short name:
AtPHAN1 Publication
Gene namesi
Name:AS11 Publication
Synonyms:MYB911 Publication, PHAN1 Publication
Ordered Locus Names:At2g37630Imported
ORF Names:F13M22.13Imported
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 2

Organism-specific databases

AraportiAT2G37630
TAIRilocus:2040671 AT2G37630

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Disruption phenotypei

Plants with double mutations in this protein and in RH10 or AS2 protein have abaxialized filamentous and trumpet-like leaves at high temperatures. In double mutants, shapes of epidermal cells of the filamentous leaves are simple and rectangular, similar to those of a petiole, but different from those of flat leaves of wild-type plants.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001970731 – 367Transcription factor AS1Add BLAST367

Proteomic databases

PaxDbiO80931
PRIDEiO80931

Expressioni

Tissue specificityi

Expressed in roots, stems, leaves, flowers, siliques and in lateral organ promordia (PubMed:17559509). Found in the inner domain between the adaxial and abaxial domains of leaves (PubMed:17559509). Expressed in the phloem tissues of leaves, cotyledons, hypocotyls, and roots (PubMed:21950734).2 Publications

Developmental stagei

Preferential expression in young and immature plant tissues. In embryos, expressed from the late globular stage onwards. After germination, detected in leaf founder cells and on flowering, in primordia of all floral organs.

Inductioni

Circadian-regulation with an afternoon peak in long days and with a broad night peak in short days (PubMed:21950734). Expression of AS1 in stem cells of the shoot apical meristem is prevented by SHOOT MERISTEMLESS (STM). Expression is activated by GTE6 during leaf morphogenesis (PubMed:16166385).2 Publications

Gene expression databases

ExpressionAtlasiO80931 baseline and differential
GenevisibleiO80931 AT

Interactioni

Subunit structurei

Homodimer (PubMed:12750468, PubMed:23271976). Heterodimer with LBD6 (PubMed:23271976). Interacts with LBD6/AS2, HIRA, a probable histone chaperone, and RIK, a predicted RNA binding protein (PubMed:12874130, PubMed:16243907). Part of the AS1 repressor complex composed of AS1, LBD6/AS2 and HDA6 (PubMed:23271976). Interacts with CO (PubMed:21950734).5 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
RCD1Q8RY592EBI-763232,EBI-2118043

GO - Molecular functioni

  • protein homodimerization activity Source: TAIR

Protein-protein interaction databases

BioGridi3684, 16 interactors
IntActiO80931, 5 interactors
STRINGi3702.AT2G37630.1

Structurei

3D structure databases

ProteinModelPortaliO80931
SMRiO80931
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini1 – 53HTH myb-type 1PROSITE-ProRule annotationAdd BLAST53
Domaini54 – 108HTH myb-type 2PROSITE-ProRule annotationAdd BLAST55

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili260 – 344Sequence analysisAdd BLAST85

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi147 – 152Poly-Ala6

Domaini

A C-terminal domain (252-356) is required for dimerization.

Keywords - Domaini

Coiled coil, Repeat

Phylogenomic databases

eggNOGiKOG0048 Eukaryota
COG5147 LUCA
HOGENOMiHOG000244041
InParanoidiO80931
KOiK09422
OMAiCRRREKM
OrthoDBiEOG09360N0R
PhylomeDBiO80931

Family and domain databases

CDDicd00167 SANT, 2 hits
InterProiView protein in InterPro
IPR009057 Homeobox-like_sf
IPR017930 Myb_dom
IPR031055 RS2/AS1
IPR001005 SANT/Myb
PANTHERiPTHR10641:SF457 PTHR10641:SF457, 1 hit
SMARTiView protein in SMART
SM00717 SANT, 2 hits
SUPFAMiSSF46689 SSF46689, 1 hit
PROSITEiView protein in PROSITE
PS51294 HTH_MYB, 2 hits

Sequencei

Sequence statusi: Complete.

O80931-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKERQRWSGE EDALLRAYVR QFGPREWHLV SERMNKPLNR DAKSCLERWK
60 70 80 90 100
NYLKPGIKKG SLTEEEQRLV IRLQEKHGNK WKKIAAEVPG RTAKRLGKWW
110 120 130 140 150
EVFKEKQQRE EKESNKRVEP IDESKYDRIL ESFAEKLVKE RSNVVPAAAA
160 170 180 190 200
AATVVMANSN GGFLHSEQQV QPPNPVIPPW LATSNNGNNV VARPPSVTLT
210 220 230 240 250
LSPSTVAAAA PQPPIPWLQQ QQPERAENGP GGLVLGSMMP SCSGSSESVF
260 270 280 290 300
LSELVECCRE LEEGHRAWAD HKKEAAWRLR RLELQLESEK TCRQREKMEE
310 320 330 340 350
IEAKMKALRE EQKNAMEKIE GEYREQLVGL RRDAEAKDQK LADQWTSRHI
360
RLTKFLEQQM GCRLDRP
Length:367
Mass (Da):42,243
Last modified:November 1, 1998 - v1
Checksum:i24D9085193E6088B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF175996 mRNA Translation: AAD53101.1
AY519578 mRNA Translation: AAS10048.1
AC004684 Genomic DNA Translation: AAC23633.1
CP002685 Genomic DNA Translation: AEC09428.1
BT026027 mRNA Translation: ABG48383.1
PIRiT02529
RefSeqiNP_181299.1, NM_129319.4
UniGeneiAt.11577

Genome annotation databases

EnsemblPlantsiAT2G37630.1; AT2G37630.1; AT2G37630
GeneIDi818340
GrameneiAT2G37630.1; AT2G37630.1; AT2G37630
KEGGiath:AT2G37630

Similar proteinsi

Entry informationi

Entry nameiAS1_ARATH
AccessioniPrimary (citable) accession number: O80931
Secondary accession number(s): Q53YQ1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 28, 2003
Last sequence update: November 1, 1998
Last modified: May 23, 2018
This is version 141 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health