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Entry version 112 (02 Dec 2020)
Sequence version 1 (01 Nov 1998)
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Protein

Spermidine sinapoyl-CoA acyltransferase

Gene

SDT

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score:

Annotation score:5 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
-Experimental evidence at protein leveli <p>This indicates the type of evidence that supports the existence of the protein. Note that the 'protein existence' evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Spermidine sinapoyl-CoA acyltransferase that mediates the accumulation of disinapoyl spermidine conjugates in seeds. Can also use putrescine as an acyl acceptor to convert it into monosinapoyl-putrescine.1 Publication

<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section describes the catalytic activity of an enzyme, i.e. a chemical reaction that the enzyme catalyzes.<p><a href='/help/catalytic_activity' target='_top'>More...</a></p>Catalytic activityi

<p>This subsection of the 'Function' section describes biophysical and chemical properties, such as maximal absorption, kinetic parameters, pH dependence, redox potentials and temperature dependence.<p><a href='/help/biophysicochemical_properties' target='_top'>More...</a></p>Kineticsi

kcat is 5.1 sec(-1) with sinapoyl-CoA as substrate (in the presence of spermidine as the acyl acceptor). kcat is 5.6 sec(-1) with spermidine as substrate (in the presence of sinapoyl-CoA as the acyl donor). kcat is 0.3 sec(-1) with putrescine as substrate (in the presence of sinapoyl-CoA as the acyl donor). kcat is 37.8 sec(-1) with disinapoyl-spermidine as substrate (in the presence of CoA as cosubstrate). kcat is 39.6 sec(-1) with CoA as substrate (in the presence of disinapoyl-spermidine as cosubstrate). All analyzes are done at 30 degrees Celsius.1 Publication
  1. KM=8.3 µM for sinapoyl-CoA (with spermidine as the acyl acceptor, at 30 degrees Celsius)1 Publication
  2. KM=37.4 µM for spermidine (with sinapoyl-CoA as the acyl donor, at 30 degrees Celsius)1 Publication
  3. KM=236.9 µM for putrescine (with sinapoyl-CoA as the acyl donor, at 30 degrees Celsius)1 Publication
  4. KM=34.6 µM for disinapoyl-spermidine (with CoA as cosubstrate, at 30 degrees Celsius)1 Publication
  5. KM=83.6 µM for CoA (with disinapoyl-spermidine as cosubstrate, at 30 degrees Celsius)1 Publication

    pH dependencei

    Optimum pH is 9 using spermidine and sinapoyl-CoA as substrates.1 Publication

    <p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">'Function'</a> section describes the metabolic pathway(s) associated with a protein.<p><a href='/help/pathway' target='_top'>More...</a></p>Pathwayi: spermidine metabolism

    This protein is involved in the pathway spermidine metabolism, which is part of Amine and polyamine metabolism.1 Publication
    View all proteins of this organism that are known to be involved in the pathway spermidine metabolism and in Amine and polyamine metabolism.

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    <p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section is used for enzymes and indicates the residues directly involved in catalysis.<p><a href='/help/act_site' target='_top'>More...</a></p>Active sitei169Proton acceptorBy similarity1
    Active sitei391Proton acceptorBy similarity1

    <p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

    GO - Biological processi

    <p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

    Molecular functionAcyltransferase, Transferase
    Biological processPolyamine biosynthesis

    Enzyme and pathway databases

    BioCyc Collection of Pathway/Genome Databases

    More...
    BioCyci
    ARA:AT2G23510-MONOMER

    UniPathway: a resource for the exploration and annotation of metabolic pathways

    More...
    UniPathwayi
    UPA00819

    <p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

    <p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
    Recommended name:
    Spermidine sinapoyl-CoA acyltransferase1 Publication (EC:2.3.1.2481 Publication)
    Short name:
    SDT1 Publication
    Short name:
    Spermidine disinapoyl transferase1 Publication
    <p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: 'Name', 'Synonyms', 'Ordered locus names' and 'ORF names'.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
    Name:SDT1 Publication
    Ordered Locus Names:At2g23510Imported
    ORF Names:F26B6.16Imported
    <p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiArabidopsis thaliana (Mouse-ear cress)Imported
    <p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the 'taxonomic identifier' or 'taxid'.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri3702 [NCBI]
    <p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliopsidaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    <p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section is present for entries that are part of a <a href="http://www.uniprot.org/proteomes">proteome</a>, i.e. of a set of proteins thought to be expressed by organisms whose genomes have been completely sequenced.<p><a href='/help/proteomes_manual' target='_top'>More...</a></p>Proteomesi
    • UP000006548 <p>A UniProt <a href="http://www.uniprot.org/manual/proteomes%5Fmanual">proteome</a> can consist of several components.<br></br>The component name refers to the genomic component encoding a set of proteins.<p><a href='/help/proteome_component' target='_top'>More...</a></p> Componenti: Chromosome 2

    Organism-specific databases

    Arabidopsis Information Portal

    More...
    Araporti
    AT2G23510

    The Arabidopsis Information Resource

    More...
    TAIRi
    locus:2046822, AT2G23510

    <p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

    <p>This subsection of the 'Pathology and Biotech' section describes the in vivo effects caused by ablation of the gene (or one or more transcripts) coding for the protein described in the entry. This includes gene knockout and knockdown, provided experiments have been performed in the context of a whole organism or a specific tissue, and not at the single-cell level.<p><a href='/help/disruption_phenotype' target='_top'>More...</a></p>Disruption phenotypei

    Impaired disinapoyl spermidine conjugates accumulation in seeds.1 Publication

    <p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    <p>This subsection of the 'PTM / Processing' section describes the extent of a polypeptide chain in the mature protein following processing or proteolytic cleavage.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_00004327711 – 451Spermidine sinapoyl-CoA acyltransferaseAdd BLAST451

    Proteomic databases

    PaxDb, a database of protein abundance averages across all three domains of life

    More...
    PaxDbi
    O80467

    PRoteomics IDEntifications database

    More...
    PRIDEi
    O80467

    ProteomicsDB: a multi-organism proteome resource

    More...
    ProteomicsDBi
    232827

    <p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

    <p>This subsection of the 'Expression' section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms. By default, the information is derived from experiments at the mRNA level, unless specified 'at protein level'.<br></br>Examples: <a href="http://www.uniprot.org/uniprot/P92958#expression">P92958</a>, <a href="http://www.uniprot.org/uniprot/Q8TDN4#expression">Q8TDN4</a>, <a href="http://www.uniprot.org/uniprot/O14734#expression">O14734</a><p><a href='/help/tissue_specificity' target='_top'>More...</a></p>Tissue specificityi

    Predominantly expressed in siliques, especially in seeds around the embryo, and, at low levels, in flowers. Barely detectable in stems, leaves, and roots.1 Publication

    <p>This subsection of the 'Expression' section provides information on the expression of the gene product at various stages of a cell, tissue or organism development. By default, the information is derived from experiments at the mRNA level, unless specified 'at the protein level'.<p><a href='/help/developmental_stage' target='_top'>More...</a></p>Developmental stagei

    Strongly expressed in the cotyledons and emerging radical of the germinating seeds one day after imbibition. Accumulates in the root tip of young seedlings and in the cotyledons and the basal region of the hypocotyl as the seedlings emerges from the seed coat three days after imbibition. Later confined to the basal region of the hypocotyls and to the root tip before progressively disappearing.1 Publication

    Gene expression databases

    ExpressionAtlas, Differential and Baseline Expression

    More...
    ExpressionAtlasi
    O80467, baseline and differential

    Genevisible search portal to normalized and curated expression data from Genevestigator

    More...
    Genevisiblei
    O80467, AT

    <p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

    <p>This subsection of the <a href="http://www.uniprot.org/help/interaction%5Fsection">'Interaction'</a> section provides information about the protein quaternary structure and interaction(s) with other proteins or protein complexes (with the exception of physiological receptor-ligand interactions which are annotated in the <a href="http://www.uniprot.org/help/function%5Fsection">'Function'</a> section).<p><a href='/help/subunit_structure' target='_top'>More...</a></p>Subunit structurei

    Monomer.

    By similarity

    Protein-protein interaction databases

    STRING: functional protein association networks

    More...
    STRINGi
    3702.AT2G23510.1

    <p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

    3D structure databases

    SWISS-MODEL Repository - a database of annotated 3D protein structure models

    More...
    SMRi
    O80467

    Database of comparative protein structure models

    More...
    ModBasei
    Search...

    <p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

    <p>This subsection of the 'Family and domains' section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

    Belongs to the plant acyltransferase family.Curated

    Phylogenomic databases

    evolutionary genealogy of genes: Non-supervised Orthologous Groups

    More...
    eggNOGi
    ENOG502RBEP, Eukaryota

    The HOGENOM Database of Homologous Genes from Fully Sequenced Organisms

    More...
    HOGENOMi
    CLU_014546_2_0_1

    InParanoid: Eukaryotic Ortholog Groups

    More...
    InParanoidi
    O80467

    Identification of Orthologs from Complete Genome Data

    More...
    OMAi
    SEPLMLM

    Database of Orthologous Groups

    More...
    OrthoDBi
    1130893at2759

    Database for complete collections of gene phylogenies

    More...
    PhylomeDBi
    O80467

    Family and domain databases

    Gene3D Structural and Functional Annotation of Protein Families

    More...
    Gene3Di
    3.30.559.10, 2 hits

    Integrated resource of protein families, domains and functional sites

    More...
    InterProi
    View protein in InterPro
    IPR023213, CAT-like_dom_sf
    IPR003480, Transferase

    Pfam protein domain database

    More...
    Pfami
    View protein in Pfam
    PF02458, Transferase, 1 hit

    <p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence%5Flength">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequencei

    <p>This subsection of the <a href="http://www.uniprot.org/help/sequences%5Fsection">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

    O80467-1 [UniParc]FASTAAdd to basket
    « Hide
            10         20         30         40         50
    MPIHIGSSIP LMVEKMLTEM VKPSKHIPQQ TLNLSTLDND PYNEVIYKAC
    60 70 80 90 100
    YVFKAKNVAD DDNRPEALLR EALSDLLGYY YPLSGSLKRQ ESDRKLQLSC
    110 120 130 140 150
    GGDGGGVPFT VATANVELSS LKNLENIDSD TALNFLPVLH VDIDGYRPFA
    160 170 180 190 200
    LQVTKFECGG FILGMAMSHA MCDGYGEGHI MCALTDLAGG KKKPMVTPIW
    210 220 230 240 250
    ERERLVGKPE DDQPPFVPGD DTAASPYLPT DDWVTEKITI RADSIRRLKE
    260 270 280 290 300
    ATLKEYDFSN ETITTFEVIG AYLWKSRVKA LNLDRDGVTV LGLSVGIRNV
    310 320 330 340 350
    VDPPLPDGYY GNAYIDMYVP LTAREVEEFT ISDIVKLIKE AKRNAHDKDY
    360 370 380 390 400
    LQEELANTEK IIKMNLTIKG KKDGLFCLTD WRNIGIFGSM DFGWDEPVNI
    410 420 430 440 450
    VPVVPSETAR TVNMFMRPSR LESDMVGGVQ IVVTLPRIAM VKFKEEMEAL

    E
    Length:451
    Mass (Da):50,377
    Last modified:November 1, 1998 - v1
    <p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:iAD66D1D7F751DE74
    GO

    Sequence databases

    Select the link destinations:

    EMBL nucleotide sequence database

    More...
    EMBLi

    GenBank nucleotide sequence database

    More...
    GenBanki

    DNA Data Bank of Japan; a nucleotide sequence database

    More...
    DDBJi
    Links Updated
    AC003040 Genomic DNA Translation: AAC23766.1
    CP002685 Genomic DNA Translation: AEC07460.1

    Protein sequence database of the Protein Information Resource

    More...
    PIRi
    T01140

    NCBI Reference Sequences

    More...
    RefSeqi
    NP_179932.1, NM_127915.2

    Genome annotation databases

    Ensembl plant genome annotation project

    More...
    EnsemblPlantsi
    AT2G23510.1; AT2G23510.1; AT2G23510

    Database of genes from NCBI RefSeq genomes

    More...
    GeneIDi
    816883

    Gramene; a comparative resource for plants

    More...
    Gramenei
    AT2G23510.1; AT2G23510.1; AT2G23510

    KEGG: Kyoto Encyclopedia of Genes and Genomes

    More...
    KEGGi
    ath:AT2G23510

    <p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

    <p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AC003040 Genomic DNA Translation: AAC23766.1
    CP002685 Genomic DNA Translation: AEC07460.1
    PIRiT01140
    RefSeqiNP_179932.1, NM_127915.2

    3D structure databases

    SMRiO80467
    ModBaseiSearch...

    Protein-protein interaction databases

    STRINGi3702.AT2G23510.1

    Proteomic databases

    PaxDbiO80467
    PRIDEiO80467
    ProteomicsDBi232827

    Genome annotation databases

    EnsemblPlantsiAT2G23510.1; AT2G23510.1; AT2G23510
    GeneIDi816883
    GrameneiAT2G23510.1; AT2G23510.1; AT2G23510
    KEGGiath:AT2G23510

    Organism-specific databases

    AraportiAT2G23510
    TAIRilocus:2046822, AT2G23510

    Phylogenomic databases

    eggNOGiENOG502RBEP, Eukaryota
    HOGENOMiCLU_014546_2_0_1
    InParanoidiO80467
    OMAiSEPLMLM
    OrthoDBi1130893at2759
    PhylomeDBiO80467

    Enzyme and pathway databases

    UniPathwayiUPA00819
    BioCyciARA:AT2G23510-MONOMER

    Miscellaneous databases

    Protein Ontology

    More...
    PROi
    PR:O80467

    Gene expression databases

    ExpressionAtlasiO80467, baseline and differential
    GenevisibleiO80467, AT

    Family and domain databases

    Gene3Di3.30.559.10, 2 hits
    InterProiView protein in InterPro
    IPR023213, CAT-like_dom_sf
    IPR003480, Transferase
    PfamiView protein in Pfam
    PF02458, Transferase, 1 hit

    ProtoNet; Automatic hierarchical classification of proteins

    More...
    ProtoNeti
    Search...

    MobiDB: a database of protein disorder and mobility annotations

    More...
    MobiDBi
    Search...

    <p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

    <p>This subsection of the 'Entry information' section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiSDT_ARATH
    <p>This subsection of the 'Entry information' section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called 'Primary (citable) accession number'.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: O80467
    <p>This subsection of the 'Entry information' section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification ('Last modified'). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 29, 2015
    Last sequence update: November 1, 1998
    Last modified: December 2, 2020
    This is version 112 of the entry and version 1 of the sequence. See complete history.
    <p>This subsection of the 'Entry information' section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    <p>This section contains any relevant information that doesn't fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

    Keywords - Technical termi

    Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families
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