UniProtKB - O80397 (M2K4_ARATH)
Protein
Mitogen-activated protein kinase kinase 4
Gene
MKK4
Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Functioni
Involved in the second phase of hydrogen peroxide generation during hypersensitive response-like cell death. Involved in the innate immune MAP kinase signaling cascade (MEKK1, MKK4/MKK5 and MPK3/MPK6) downstream of bacterial flagellin receptor FLS2. Activates by phosphorylation the downstream MPK3 and MPK6. YDA-MKK4/MKK5-MPK3/MPK6 module regulates stomatal cell fate before the guard mother cell (GMC) is specified. This MAPK cascade also functions downstream of the ER receptor in regulating coordinated local cell proliferation, which shapes the morphology of plant organs. MKK4 and MKK5 participate in the regulation of floral organ abscission. Mediates osmotic-stress response via its regulation of MPK3 activity. Target of the Pseudomonas syringae type III effector HopF2.7 Publications
Catalytic activityi
Activity regulationi
Activated through serine and threonine phosphorylation by MEKK1. Inhibited through phosphorylation by GSK3/Shaggy-like kinase ASKs.2 Publications
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 108 | ATPPROSITE-ProRule annotation | 1 | |
Active sitei | 196 | Proton acceptorPROSITE-ProRule annotation | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 85 – 93 | ATPPROSITE-ProRule annotation | 9 |
GO - Molecular functioni
- ATP binding Source: UniProtKB-KW
- protein serine/threonine kinase activity Source: GO_Central
GO - Biological processi
- activation of protein kinase activity Source: GO_Central
- defense response, incompatible interaction Source: TAIR
- floral organ abscission Source: TAIR
- inflorescence development Source: TAIR
- plant-type hypersensitive response Source: UniProtKB-KW
- signal transduction by protein phosphorylation Source: GO_Central
Keywordsi
Molecular function | Kinase, Serine/threonine-protein kinase, Transferase |
Biological process | Hypersensitive response, Plant defense |
Ligand | ATP-binding, Nucleotide-binding |
Names & Taxonomyi
Protein namesi | Recommended name: Mitogen-activated protein kinase kinase 4 (EC:2.7.12.2)Short name: AtMKK4 Short name: MAP kinase kinase 4 |
Gene namesi | Name:MKK4 Ordered Locus Names:At1g51660 ORF Names:F19C24.26 |
Organismi | Arabidopsis thaliana (Mouse-ear cress) |
Taxonomic identifieri | 3702 [NCBI] |
Taxonomic lineagei | Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliopsida › eudicotyledons › Gunneridae › Pentapetalae › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis |
Proteomesi |
|
Organism-specific databases
Araporti | AT1G51660 |
TAIRi | locus:2017662 AT1G51660 |
Subcellular locationi
Nucleus
Chloroplast
Other locations
Chloroplast
- chloroplast stroma Source: UniProtKB
Nucleus
- nucleus Source: TAIR
Other locations
- cytoplasm Source: TAIR
Keywords - Cellular componenti
Chloroplast, Cytoplasm, Nucleus, PlastidPathology & Biotechi
Disruption phenotypei
RNAi double mutants MKK4 and MKK5 have a strong abscission defect. Higher sensitivity to high salt and drought stresses.2 Publications
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 108 | K → M: Loss of activity. 2 Publications | 1 | |
Mutagenesisi | 108 | K → R: Loss of activity. Phosphorylated by MAPKKK5. 3 Publications | 1 | |
Mutagenesisi | 224 | T → A: Impaired phosphorylation by MAPKKK5; when associated with A-230. 1 Publication | 1 | |
Mutagenesisi | 224 | T → D: Constitutively active; when associated with D-230 or E-230. 2 Publications | 1 | |
Mutagenesisi | 230 | S → A: Abolishes phosphorylation by ASK7/BIN2. Impaired phosphorylation by MAPKKK5; when associated with A-224. 4 Publications | 1 | |
Mutagenesisi | 230 | S → D: Constitutively active; when associated with D-224. 3 Publications | 1 | |
Mutagenesisi | 230 | S → E: Constitutively active; when associated with D-224. 3 Publications | 1 | |
Mutagenesisi | 234 | T → A: Abolishes phosphorylation by ASK7/BIN2. 1 Publication | 1 | |
Mutagenesisi | 234 | T → D or E: Abolishes activity of MKK4 against MPK6. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000245823 | 1 – 366 | Mitogen-activated protein kinase kinase 4Add BLAST | 366 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 6 | Phosphoserine; by ASK71 Publication | 1 | |
Modified residuei | 224 | Phosphothreonine1 Publication | 1 | |
Modified residuei | 230 | Phosphoserine2 Publications | 1 | |
Modified residuei | 230 | Phosphoserine; by ASK72 Publications | 1 | |
Modified residuei | 234 | Phosphothreonine; by ASK71 Publication | 1 |
Post-translational modificationi
Phosphorylation at Thr-224 and Ser-230 by MAP kinase kinase kinases positively regulates kinase activity. Phosphorylation at Ser-230 and Thr-234 by GSK3/Shaggy-like kinase ASKs negatively regulates kinase activity. Phosphorylated by MAPKKK5 (PubMed:27679653).3 Publications
Keywords - PTMi
PhosphoproteinProteomic databases
PaxDbi | O80397 |
PRIDEi | O80397 |
PTM databases
iPTMneti | O80397 |
Expressioni
Tissue specificityi
Expressed higher in stems and leaves than in flowers and roots.2 Publications
Gene expression databases
ExpressionAtlasi | O80397 baseline and differential |
Genevisiblei | O80397 AT |
Interactioni
Subunit structurei
Protein-protein interaction databases
BioGridi | 26816, 15 interactors |
IntActi | O80397, 10 interactors |
STRINGi | 3702.AT1G51660.1 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 79 – 334 | Protein kinasePROSITE-ProRule annotationAdd BLAST | 256 |
Sequence similaritiesi
Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase subfamily.Curated
Phylogenomic databases
eggNOGi | KOG0581 Eukaryota ENOG410XQ5A LUCA |
HOGENOMi | HOG000234206 |
InParanoidi | O80397 |
KOi | K13413 |
OMAi | CHDMYER |
OrthoDBi | 688282at2759 |
PhylomeDBi | O80397 |
Family and domain databases
InterProi | View protein in InterPro IPR011009 Kinase-like_dom_sf IPR000719 Prot_kinase_dom IPR017441 Protein_kinase_ATP_BS IPR008271 Ser/Thr_kinase_AS |
Pfami | View protein in Pfam PF00069 Pkinase, 1 hit |
SMARTi | View protein in SMART SM00220 S_TKc, 1 hit |
SUPFAMi | SSF56112 SSF56112, 1 hit |
PROSITEi | View protein in PROSITE PS00107 PROTEIN_KINASE_ATP, 1 hit PS50011 PROTEIN_KINASE_DOM, 1 hit PS00108 PROTEIN_KINASE_ST, 1 hit |
i Sequence
Sequence statusi: Complete.
O80397-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MRPIQSPPGV SVPVKSRPRR RPDLTLPLPQ RDVSLAVPLP LPPTSGGSGG
60 70 80 90 100
SSGSAPSSGG SASSTNTNSS IEAKNYSDLV RGNRIGSGAG GTVYKVIHRP
110 120 130 140 150
SSRLYALKVI YGNHEETVRR QICREIEILR DVNHPNVVKC HEMFDQNGEI
160 170 180 190 200
QVLLEFMDKG SLEGAHVWKE QQLADLSRQI LSGLAYLHSR HIVHRDIKPS
210 220 230 240 250
NLLINSAKNV KIADFGVSRI LAQTMDPCNS SVGTIAYMSP ERINTDLNQG
260 270 280 290 300
KYDGYAGDIW SLGVSILEFY LGRFPFPVSR QGDWASLMCA ICMSQPPEAP
310 320 330 340 350
ATASPEFRHF ISCCLQREPG KRRSAMQLLQ HPFILRASPS QNRSPQNLHQ
360
LLPPPRPLSS SSSPTT
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB015315 mRNA Translation: BAA28830.1 AC025294 Genomic DNA Translation: AAG50863.1 CP002684 Genomic DNA Translation: AEE32696.1 AF324667 mRNA Translation: AAG40018.1 AF326878 mRNA Translation: AAG41460.1 AF349517 mRNA Translation: AAK15564.1 AF375398 mRNA Translation: AAK52982.1 AY129469 mRNA Translation: AAM91055.1 |
PIRi | T51339 |
RefSeqi | NP_175577.1, NM_104044.3 |
Genome annotation databases
EnsemblPlantsi | AT1G51660.1; AT1G51660.1; AT1G51660 |
GeneIDi | 841591 |
Gramenei | AT1G51660.1; AT1G51660.1; AT1G51660 |
KEGGi | ath:AT1G51660 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB015315 mRNA Translation: BAA28830.1 AC025294 Genomic DNA Translation: AAG50863.1 CP002684 Genomic DNA Translation: AEE32696.1 AF324667 mRNA Translation: AAG40018.1 AF326878 mRNA Translation: AAG41460.1 AF349517 mRNA Translation: AAK15564.1 AF375398 mRNA Translation: AAK52982.1 AY129469 mRNA Translation: AAM91055.1 |
PIRi | T51339 |
RefSeqi | NP_175577.1, NM_104044.3 |
3D structure databases
SMRi | O80397 |
ModBasei | Search... |
Protein-protein interaction databases
BioGridi | 26816, 15 interactors |
IntActi | O80397, 10 interactors |
STRINGi | 3702.AT1G51660.1 |
PTM databases
iPTMneti | O80397 |
Proteomic databases
PaxDbi | O80397 |
PRIDEi | O80397 |
Genome annotation databases
EnsemblPlantsi | AT1G51660.1; AT1G51660.1; AT1G51660 |
GeneIDi | 841591 |
Gramenei | AT1G51660.1; AT1G51660.1; AT1G51660 |
KEGGi | ath:AT1G51660 |
Organism-specific databases
Araporti | AT1G51660 |
TAIRi | locus:2017662 AT1G51660 |
Phylogenomic databases
eggNOGi | KOG0581 Eukaryota ENOG410XQ5A LUCA |
HOGENOMi | HOG000234206 |
InParanoidi | O80397 |
KOi | K13413 |
OMAi | CHDMYER |
OrthoDBi | 688282at2759 |
PhylomeDBi | O80397 |
Miscellaneous databases
PROi | PR:O80397 |
Gene expression databases
ExpressionAtlasi | O80397 baseline and differential |
Genevisiblei | O80397 AT |
Family and domain databases
InterProi | View protein in InterPro IPR011009 Kinase-like_dom_sf IPR000719 Prot_kinase_dom IPR017441 Protein_kinase_ATP_BS IPR008271 Ser/Thr_kinase_AS |
Pfami | View protein in Pfam PF00069 Pkinase, 1 hit |
SMARTi | View protein in SMART SM00220 S_TKc, 1 hit |
SUPFAMi | SSF56112 SSF56112, 1 hit |
PROSITEi | View protein in PROSITE PS00107 PROTEIN_KINASE_ATP, 1 hit PS50011 PROTEIN_KINASE_DOM, 1 hit PS00108 PROTEIN_KINASE_ST, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | M2K4_ARATH | |
Accessioni | O80397Primary (citable) accession number: O80397 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 11, 2006 |
Last sequence update: | November 1, 1998 | |
Last modified: | October 16, 2019 | |
This is version 147 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Plant Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Complete proteome, Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families - Arabidopsis thaliana
Arabidopsis thaliana: entries and gene names