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Protein

Decapping and exoribonuclease protein

Gene

DXO

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Ribonuclease that specifically degrades pre-mRNAs with a defective 5' end cap and is part of a pre-mRNA capping quality control. Has decapping, pyrophosphohydrolase and 5'-3' exonuclease activities. Has decapping activity toward incomplete 5' end cap mRNAs such as unmethylated 5' end-capped RNA to release GpppN and 5' end monophosphate RNA. The 5' end monophosphate RNA is then degraded by the 5'-3' exoribonuclease activity, enabling this enzyme to decap and degrade incompletely capped mRNAs. Also possesses RNA 5'-pyrophosphohydrolase activity by hydrolyzing the 5' end triphosphate to release pyrophosphates (By similarity).By similarity

Caution

In contrast to canonical decapping enzymes DCP2 and NUDT16, which release m7pppG (m7GDP), the decapping activity releases the entire cap structure GpppN and a 5' end monophosphate RNA.Curated

Cofactori

Mg2+By similarityNote: Binds 2 magnesium ions.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei58SubstrateBy similarity1
Binding sitei101SubstrateBy similarity1
Binding sitei132SubstrateBy similarity1
Metal bindingi192Magnesium 1By similarity1
Metal bindingi192Magnesium 2By similarity1
Binding sitei217SubstrateBy similarity1
Metal bindingi234Magnesium 2By similarity1
Binding sitei234SubstrateBy similarity1
Metal bindingi236Magnesium 1By similarity1
Metal bindingi236Magnesium 2By similarity1
Metal bindingi253Magnesium 1By similarity1
Metal bindingi254Magnesium 1; via carbonyl oxygenBy similarity1
Binding sitei280SubstrateBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionExonuclease, Hydrolase, Nuclease, RNA-binding
LigandMagnesium, Metal-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Decapping and exoribonuclease protein (EC:3.1.13.-, EC:3.6.1.-)
Short name:
DXO
Alternative name(s):
Dom-3 homolog Z
Gene namesi
Name:DXO
Synonyms:DOM3L, DOM3Z, NG6
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 6

Organism-specific databases

EuPathDBiHostDB:ENSG00000204348.9
HGNCiHGNC:2992 DXO
MIMi605996 gene
neXtProtiNX_O77932

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Organism-specific databases

DisGeNETi1797
OpenTargetsiENSG00000204348
PharmGKBiPA27458

Polymorphism and mutation databases

BioMutaiDOM3Z

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002498221 – 396Decapping and exoribonuclease proteinAdd BLAST396

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei392PhosphothreonineBy similarity1
Modified residuei394PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiO77932
MaxQBiO77932
PaxDbiO77932
PeptideAtlasiO77932
PRIDEiO77932
ProteomicsDBi50418

PTM databases

iPTMnetiO77932
PhosphoSitePlusiO77932

Expressioni

Tissue specificityi

Ubiquitously expressed.1 Publication

Gene expression databases

BgeeiENSG00000204348 Expressed in 221 organ(s), highest expression level in right adrenal gland
CleanExiHS_DOM3Z
ExpressionAtlasiO77932 baseline and differential
GenevisibleiO77932 HS

Organism-specific databases

HPAiHPA046708

Interactioni

Protein-protein interaction databases

BioGridi108132, 15 interactors
IntActiO77932, 8 interactors
MINTiO77932
STRINGi9606.ENSP00000337759

Structurei

3D structure databases

ProteinModelPortaliO77932
SMRiO77932
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the DXO/Dom3Z family.Curated

Phylogenomic databases

eggNOGiKOG1982 Eukaryota
ENOG410ZAFR LUCA
GeneTreeiENSGT00390000006425
HOVERGENiHBG080627
InParanoidiO77932
KOiK14845
OMAiMAYWGYK
OrthoDBiEOG091G0F0X
PhylomeDBiO77932
TreeFamiTF322812

Family and domain databases

InterProiView protein in InterPro
IPR013961 RAI1
IPR039039 RAI1-like_fam
PANTHERiPTHR12395 PTHR12395, 1 hit
PfamiView protein in Pfam
PF08652 RAI1, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

This entry has 1 described isoform and 2 potential isoforms that are computationally mapped.Show allAlign All

O77932-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MDPRGTKRGA EKTEVAEPRN KLPRPAPSLP TDPALYSGPF PFYRRPSELG
60 70 80 90 100
CFSLDAQRQY HGDARALRYY SPPPTNGPGP NFDLRDGYPD RYQPRDEEVQ
110 120 130 140 150
ERLDHLLCWL LEHRGRLEGG PGWLAEAIVT WRGHLTKLLT TPYERQEGWQ
160 170 180 190 200
LAASRFQGTL YLSEVETPNA RAQRLARPPL LRELMYMGYK FEQYMCADKP
210 220 230 240 250
GSSPDPSGEV NTNVAFCSVL RSRLGSHPLL FSGEVDCTDP QAPSTQPPTC
260 270 280 290 300
YVELKTSKEM HSPGQWRSFY RHKLLKWWAQ SFLPGVPNVV AGFRNPDGFV
310 320 330 340 350
SSLKTFPTMK MFEYVRNDRD GWNPSVCMNF CAAFLSFAQS TVVQDDPRLV
360 370 380 390
HLFSWEPGGP VTVSVHQDAP YAFLPIWYVE AMTQDLPSPP KTPSPK
Length:396
Mass (Da):44,929
Last modified:September 19, 2006 - v2
Checksum:i17C8119037EB6892
GO

Computationally mapped potential isoform sequencesi

There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
F8WC68F8WC68_HUMAN
Decapping and exoribonuclease prote...
DXO
210Annotation score:
H7C5D2H7C5D2_HUMAN
Decapping and exoribonuclease prote...
DXO
174Annotation score:

Sequence cautioni

The sequence AAB67983 differs from that shown. Reason: Erroneous gene model prediction.Curated
The sequence CAB89306 differs from that shown. Reason: Erroneous gene model prediction.Curated

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_02749228S → T2 PublicationsCorresponds to variant dbSNP:rs1056694Ensembl.1
Natural variantiVAR_02749363D → E. Corresponds to variant dbSNP:rs2746396Ensembl.1
Natural variantiVAR_027494261H → Q1 PublicationCorresponds to variant dbSNP:rs17207867Ensembl.1
Natural variantiVAR_027495332A → V. Corresponds to variant dbSNP:rs12205138Ensembl.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF059252 mRNA Translation: AAC78603.1
AF019413 Genomic DNA Translation: AAB67983.1 Sequence problems.
AL049547 Genomic DNA Translation: CAB89305.1
AL049547 Genomic DNA Translation: CAB89306.1 Sequence problems.
AL662849 Genomic DNA No translation available.
AL844853 Genomic DNA No translation available.
CR753822 Genomic DNA No translation available.
CR759782 Genomic DNA No translation available.
AL645922 Genomic DNA No translation available.
CR753845 Genomic DNA No translation available.
CH471081 Genomic DNA Translation: EAX03558.1
BC009344 mRNA Translation: AAH09344.1
BC019083 mRNA Translation: AAH19083.1
AF059253 mRNA Translation: AAC78604.1
AF059254 mRNA Translation: AAC78605.1
CCDSiCCDS4732.1
RefSeqiNP_005501.2, NM_005510.3
XP_006715068.1, XM_006715005.3
UniGeneiHs.153299

Genome annotation databases

EnsembliENST00000337523; ENSP00000337759; ENSG00000204348
ENST00000375349; ENSP00000364498; ENSG00000204348
ENST00000375356; ENSP00000364505; ENSG00000204348
ENST00000383330; ENSP00000372820; ENSG00000206346
ENST00000399936; ENSP00000382818; ENSG00000206346
ENST00000399937; ENSP00000382819; ENSG00000206346
ENST00000414092; ENSP00000391123; ENSG00000236765
ENST00000419797; ENSP00000391532; ENSG00000234798
ENST00000426498; ENSP00000394167; ENSG00000225682
ENST00000427627; ENSP00000413860; ENSG00000234798
ENST00000427690; ENSP00000406143; ENSG00000225682
ENST00000432110; ENSP00000401315; ENSG00000236765
ENST00000439989; ENSP00000395262; ENSG00000224313
ENST00000443250; ENSP00000415675; ENSG00000224313
ENST00000446967; ENSP00000391349; ENSG00000234798
ENST00000453742; ENSP00000410404; ENSG00000225682
ENST00000454430; ENSP00000411380; ENSG00000224313
ENST00000458308; ENSP00000400173; ENSG00000236765
GeneIDi1797
KEGGihsa:1797
UCSCiuc003nyp.2 human

Keywords - Coding sequence diversityi

Polymorphism

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF059252 mRNA Translation: AAC78603.1
AF019413 Genomic DNA Translation: AAB67983.1 Sequence problems.
AL049547 Genomic DNA Translation: CAB89305.1
AL049547 Genomic DNA Translation: CAB89306.1 Sequence problems.
AL662849 Genomic DNA No translation available.
AL844853 Genomic DNA No translation available.
CR753822 Genomic DNA No translation available.
CR759782 Genomic DNA No translation available.
AL645922 Genomic DNA No translation available.
CR753845 Genomic DNA No translation available.
CH471081 Genomic DNA Translation: EAX03558.1
BC009344 mRNA Translation: AAH09344.1
BC019083 mRNA Translation: AAH19083.1
AF059253 mRNA Translation: AAC78604.1
AF059254 mRNA Translation: AAC78605.1
CCDSiCCDS4732.1
RefSeqiNP_005501.2, NM_005510.3
XP_006715068.1, XM_006715005.3
UniGeneiHs.153299

3D structure databases

ProteinModelPortaliO77932
SMRiO77932
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi108132, 15 interactors
IntActiO77932, 8 interactors
MINTiO77932
STRINGi9606.ENSP00000337759

PTM databases

iPTMnetiO77932
PhosphoSitePlusiO77932

Polymorphism and mutation databases

BioMutaiDOM3Z

Proteomic databases

EPDiO77932
MaxQBiO77932
PaxDbiO77932
PeptideAtlasiO77932
PRIDEiO77932
ProteomicsDBi50418

Protocols and materials databases

DNASUi1797
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000337523; ENSP00000337759; ENSG00000204348
ENST00000375349; ENSP00000364498; ENSG00000204348
ENST00000375356; ENSP00000364505; ENSG00000204348
ENST00000383330; ENSP00000372820; ENSG00000206346
ENST00000399936; ENSP00000382818; ENSG00000206346
ENST00000399937; ENSP00000382819; ENSG00000206346
ENST00000414092; ENSP00000391123; ENSG00000236765
ENST00000419797; ENSP00000391532; ENSG00000234798
ENST00000426498; ENSP00000394167; ENSG00000225682
ENST00000427627; ENSP00000413860; ENSG00000234798
ENST00000427690; ENSP00000406143; ENSG00000225682
ENST00000432110; ENSP00000401315; ENSG00000236765
ENST00000439989; ENSP00000395262; ENSG00000224313
ENST00000443250; ENSP00000415675; ENSG00000224313
ENST00000446967; ENSP00000391349; ENSG00000234798
ENST00000453742; ENSP00000410404; ENSG00000225682
ENST00000454430; ENSP00000411380; ENSG00000224313
ENST00000458308; ENSP00000400173; ENSG00000236765
GeneIDi1797
KEGGihsa:1797
UCSCiuc003nyp.2 human

Organism-specific databases

CTDi1797
DisGeNETi1797
EuPathDBiHostDB:ENSG00000204348.9
GeneCardsiDXO
H-InvDBiHIX0166417
HGNCiHGNC:2992 DXO
HPAiHPA046708
MIMi605996 gene
neXtProtiNX_O77932
OpenTargetsiENSG00000204348
PharmGKBiPA27458
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG1982 Eukaryota
ENOG410ZAFR LUCA
GeneTreeiENSGT00390000006425
HOVERGENiHBG080627
InParanoidiO77932
KOiK14845
OMAiMAYWGYK
OrthoDBiEOG091G0F0X
PhylomeDBiO77932
TreeFamiTF322812

Miscellaneous databases

ChiTaRSiDXO human
GeneWikiiDOM3Z
GenomeRNAii1797
PROiPR:O77932
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000204348 Expressed in 221 organ(s), highest expression level in right adrenal gland
CleanExiHS_DOM3Z
ExpressionAtlasiO77932 baseline and differential
GenevisibleiO77932 HS

Family and domain databases

InterProiView protein in InterPro
IPR013961 RAI1
IPR039039 RAI1-like_fam
PANTHERiPTHR12395 PTHR12395, 1 hit
PfamiView protein in Pfam
PF08652 RAI1, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiDXO_HUMAN
AccessioniPrimary (citable) accession number: O77932
Secondary accession number(s): A2CER3
, B0UZ80, O15004, O78127, O78128, Q5ST60, Q6IPZ2, Q9NPK4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 19, 2006
Last sequence update: September 19, 2006
Last modified: November 7, 2018
This is version 128 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. Human chromosome 6
    Human chromosome 6: entries, gene names and cross-references to MIM
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