UniProtKB - O77819 (ROCK1_RABIT)
Rho-associated protein kinase 1
ROCK1
Functioni
Protein kinase which is a key regulator of the actin cytoskeleton and cell polarity (By similarity).
Involved in regulation of smooth muscle contraction, actin cytoskeleton organization, stress fiber and focal adhesion formation, neurite retraction, cell adhesion and motility via phosphorylation of DAPK3, GFAP, LIMK1, LIMK2, MYL9/MLC2, TPPP, PFN1 and PPP1R12A (By similarity) (PubMed:9139666).
Phosphorylates FHOD1 and acts synergistically with it to promote SRC-dependent non-apoptotic plasma membrane blebbing. Phosphorylates JIP3 and regulates the recruitment of JNK to JIP3 upon UVB-induced stress (By similarity).
Acts as a suppressor of inflammatory cell migration by regulating PTEN phosphorylation and stability (By similarity).
Acts as a negative regulator of VEGF-induced angiogenic endothelial cell activation. Required for centrosome positioning and centrosome-dependent exit from mitosis (By similarity).
Plays a role in terminal erythroid differentiation (By similarity).
Inhibits podocyte motility via regulation of actin cytoskeletal dynamics and phosphorylation of CFL1 (By similarity).
Promotes keratinocyte terminal differentiation (By similarity).
Involved in osteoblast compaction through the fibronectin fibrillogenesis cell-mediated matrix assembly process, essential for osteoblast mineralization (By similarity).
May regulate closure of the eyelids and ventral body wall by inducing the assembly of actomyosin bundles (By similarity).
By similarity1 PublicationCatalytic activityi
- EC:2.7.11.1By similarityThis reaction proceeds in the forwardBy similarity direction.
- EC:2.7.11.1By similarityThis reaction proceeds in the forwardBy similarity direction.
Cofactori
Activity regulationi
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 105 | ATPPROSITE-ProRule annotation | 1 | |
Active sitei | 198 | Proton acceptorPROSITE-ProRule annotation | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 82 – 90 | ATPPROSITE-ProRule annotation | 9 | |
Zinc fingeri | 1228 – 1283 | Phorbol-ester/DAG-typePROSITE-ProRule annotationAdd BLAST | 56 |
GO - Molecular functioni
- ATP binding Source: UniProtKB-KW
- metal ion binding Source: UniProtKB-KW
- protein serine/threonine/tyrosine kinase activity Source: RHEA
- protein serine/threonine kinase activity Source: UniProtKB
- protein serine kinase activity Source: RHEA
- small GTPase binding Source: InterPro
GO - Biological processi
- actin cytoskeleton organization Source: InterPro
- apoptotic process Source: UniProtKB-KW
- glomerular visceral epithelial cell migration Source: UniProtKB
- myoblast migration Source: UniProtKB
- negative regulation of phosphorylation Source: UniProtKB
- positive regulation of focal adhesion assembly Source: UniProtKB
- protein phosphorylation Source: UniProtKB
- regulation of actin cytoskeleton organization Source: UniProtKB
- regulation of cell migration Source: UniProtKB
- regulation of microtubule cytoskeleton organization Source: UniProtKB
- Rho protein signal transduction Source: InterPro
Keywordsi
Molecular function | Kinase, Serine/threonine-protein kinase, Transferase |
Biological process | Apoptosis |
Ligand | ATP-binding, Magnesium, Metal-binding, Nucleotide-binding, Zinc |
Names & Taxonomyi
Protein namesi | Recommended name: Rho-associated protein kinase 1 (EC:2.7.11.1By similarity)Alternative name(s): Corneal epithelial Rho-associated-Ser/Thr kinase 1 HEBM1 Rho-associated, coiled-coil-containing protein kinase 1 Rho-associated, coiled-coil-containing protein kinase I Short name: ROCK-I cAMP-dependent protein kinase ROCK-I Short name: CePKA p160 ROCK-1 Short name: p160ROCK |
Gene namesi | Name:ROCK1 |
Organismi | Oryctolagus cuniculus (Rabbit) |
Taxonomic identifieri | 9986 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Lagomorpha › Leporidae › Oryctolagus |
Proteomesi |
|
Subcellular locationi
Cytoskeleton
- centriole By similarity
- cytoskeleton By similarity
Golgi apparatus
- Golgi apparatus membrane By similarity; Peripheral membrane protein By similarity
Cytoplasm and Cytosol
- Cytoplasm By similarity
Plasma membrane
- Cell membrane By similarity
Other locations
- bleb By similarity
- lamellipodium By similarity
- ruffle By similarity
Note: A small proportion is associated with Golgi membranes (By similarity). Associated with the mother centriole and an intercentriolar linker (By similarity). Colocalizes with ITGB1BP1 and ITGB1 at the cell membrane predominantly in lamellipodia and membrane ruffles, but also in retraction fibers (By similarity). Localizes at the cell membrane in an ITGB1BP1-dependent manner (By similarity).By similarity
Cytoskeleton
- centriole Source: UniProtKB-SubCell
- cytoskeleton Source: UniProtKB
Golgi apparatus
- Golgi membrane Source: UniProtKB-SubCell
Plasma Membrane
- plasma membrane Source: UniProtKB
Other locations
- bleb Source: UniProtKB-SubCell
- lamellipodium Source: UniProtKB
- ruffle Source: UniProtKB
Keywords - Cellular componenti
Cell membrane, Cell projection, Cytoplasm, Cytoskeleton, Golgi apparatus, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | RemovedBy similarity | |||
ChainiPRO_0000086622 | 2 – 1354 | Rho-associated protein kinase 1Add BLAST | 1353 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 2 | N-acetylserineBy similarity | 1 | |
Modified residuei | 1105 | PhosphoserineBy similarity | 1 | |
Modified residuei | 1108 | PhosphoserineBy similarity | 1 | |
Modified residuei | 1328 | PhosphoserineBy similarity | 1 |
Post-translational modificationi
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sitei | 1113 – 1114 | Cleavage; by caspase-3By similarity | 2 |
Keywords - PTMi
Acetylation, PhosphoproteinExpressioni
Tissue specificityi
Interactioni
Subunit structurei
Homodimer.
Interacts with RHOA (activated by GTP), RHOB, RHOC, GEM, MYLC2B, RHOE, PPP1R12A, LIMK1, LIMK2, TSG101, CHORDC1, DAPK3, PFN1, PTEN and JIP3.
Interacts with FHOD1 in a Src-dependent manner.
Interacts with ITGB1BP1 (via N-terminus and PTB domain).
Interacts with SHROOM3 (By similarity).
By similarityGO - Molecular functioni
- small GTPase binding Source: InterPro
Protein-protein interaction databases
STRINGi | 9986.ENSOCUP00000004197 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 76 – 338 | Protein kinasePROSITE-ProRule annotationAdd BLAST | 263 | |
Domaini | 341 – 409 | AGC-kinase C-terminalPROSITE-ProRule annotationAdd BLAST | 69 | |
Domaini | 479 – 556 | REM-1PROSITE-ProRule annotationAdd BLAST | 78 | |
Domaini | 949 – 1015 | RhoBDPROSITE-ProRule annotationAdd BLAST | 67 | |
Domaini | 1118 – 1317 | PHPROSITE-ProRule annotationAdd BLAST | 200 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 368 – 727 | Interaction with FHOD1By similarityAdd BLAST | 360 | |
Regioni | 707 – 946 | SHROOM3 bindingBy similarityAdd BLAST | 240 | |
Regioni | 998 – 1010 | RHOA bindingBy similarityAdd BLAST | 13 | |
Regioni | 1101 – 1120 | DisorderedSequence analysisAdd BLAST | 20 | |
Regioni | 1115 – 1354 | Auto-inhibitoryBy similarityAdd BLAST | 240 | |
Regioni | 1333 – 1354 | DisorderedSequence analysisAdd BLAST | 22 |
Coiled coil
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Coiled coili | 422 – 692 | Sequence analysisAdd BLAST | 271 | |
Coiled coili | 1011 – 1102 | Sequence analysisAdd BLAST | 92 |
Domaini
Sequence similaritiesi
Zinc finger
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 1228 – 1283 | Phorbol-ester/DAG-typePROSITE-ProRule annotationAdd BLAST | 56 |
Keywords - Domaini
Coiled coil, Zinc-fingerPhylogenomic databases
eggNOGi | KOG0612, Eukaryota |
InParanoidi | O77819 |
OrthoDBi | 759391at2759 |
Family and domain databases
CDDi | cd00029, C1, 1 hit cd11639, HR1_ROCK1, 1 hit |
Gene3Di | 2.30.29.30, 1 hit |
InterProi | View protein in InterPro IPR000961, AGC-kinase_C IPR046349, C1-like_sf IPR011072, HR1_rho-bd IPR011009, Kinase-like_dom_sf IPR002219, PE/DAG-bd IPR011993, PH-like_dom_sf IPR001849, PH_domain IPR000719, Prot_kinase_dom IPR017441, Protein_kinase_ATP_BS IPR020684, ROCK1/ROCK2 IPR037310, ROCK1_HR1 IPR015008, ROCK_Rho-bd_dom IPR008271, Ser/Thr_kinase_AS |
Pfami | View protein in Pfam PF00069, Pkinase, 1 hit PF08912, Rho_Binding, 1 hit |
PIRSFi | PIRSF037568, Rho_kinase, 1 hit |
SMARTi | View protein in SMART SM00109, C1, 1 hit SM00233, PH, 1 hit SM00133, S_TK_X, 1 hit SM00220, S_TKc, 1 hit |
SUPFAMi | SSF56112, SSF56112, 1 hit SSF57889, SSF57889, 1 hit |
PROSITEi | View protein in PROSITE PS51285, AGC_KINASE_CTER, 1 hit PS50003, PH_DOMAIN, 1 hit PS00107, PROTEIN_KINASE_ATP, 1 hit PS50011, PROTEIN_KINASE_DOM, 1 hit PS00108, PROTEIN_KINASE_ST, 1 hit PS51860, REM_1, 1 hit PS51859, RHO_BD, 1 hit PS50081, ZF_DAG_PE_2, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
10 20 30 40 50
MSTGDSFETR FEKIDNLLRD PKSEVNSDCL LDGLDALVYD LDFPALRKNK
60 70 80 90 100
NIDNFLSRYK DTINKIRDLR MKAEDYEVVK VIGRGAFGEV QLVRHKSTRK
110 120 130 140 150
VYAMKLLSKF EMIKRSDSAF FWEERDIMAF ANSPWVVQLF YAFQDDRYLY
160 170 180 190 200
MVMEYMPGGD LVNLMSNYDV PEKWARFYTA EVVLALDAIH SMGFIHRDVK
210 220 230 240 250
PDNMLLDKSG HLKLADFGTC MKMNKEGMVR CDTAVGTPDY ISPEVLKSQG
260 270 280 290 300
GDGYYGRECD WWSVGVFLYE MLVGDTPFYA DSLVGTYSKI MNHKNSLTFP
310 320 330 340 350
DDNDISKEAK NLICAFLTDR EVRLGRNGVE EIKRHLFFKN DQWAWETLRD
360 370 380 390 400
TVAPVVPDLS SDIDTSNFDD LEEDKGDEET FPIPKAFVGN QLPFVGFTYY
410 420 430 440 450
SNRRYLSPAN PNDNRTSSNV DKSLQENLQK TIYKLEEQLH NEMQLKDEME
460 470 480 490 500
QKCRTSNIKL DKIMKELDEE GNQRRNLEST VSQIEKEKML LQHRINEYQR
510 520 530 540 550
KAEQENEKRR NVENEVSTLK DQLEDLKKVS QNSQLANEKL AQLQKQLEEA
560 570 580 590 600
NDLLRTESDT AVRLRKSHTE MSKSISQLES LNRELQERNR ILENSKSQTD
610 620 630 640 650
KDYYQLQAVL EAERRDRGHD SEMIGDLQAR ITSLQEEVKH LKYNLERMEG
660 670 680 690 700
ERKEAQDMLN HSEKEKNNLE IDLNYKLKSL QQRLEQEVNE HKVTKARLTD
710 720 730 740 750
KHQSIEEAKS VAMCEMEKKL KEEREAREKA ENRVVQIEKQ CSMLDVDLKQ
760 770 780 790 800
SQQKLEHLTE NKERMEDEVK NLTLQLEQES NKRLLLQNEL KTQAFEADNL
810 820 830 840 850
KGLEKQMKQE INTLLEAKRL LEFELAQLTK QYRGNEGQMR ELQDQLEAEQ
860 870 880 890 900
YFSTLYKTQV KELKEEIEEK NRENLKKIQE LQNEKETLAT QLDLAETKAE
910 920 930 940 950
SEQLARGLLE EQYFELTQES KKAASRNRQE ITDKDHAVSR LEETNSILTK
960 970 980 990 1000
DIELLRKENE ELTDKMRKSE EEYKLQKEEE ISNLKATYEK NINTERTLKT
1010 1020 1030 1040 1050
QAVNKLAEIM NRKDFKIDKK KANTQDLRKK EKENRKLQLE LNQEREKFNQ
1060 1070 1080 1090 1100
MVVKHQKELN DMQAQLVEEC THRNELQMQL ASKESDIEQL RAKLSDLSDS
1110 1120 1130 1140 1150
TSVASFPSAD ETDPNLPESR IEGWLSVPNR GNIKRYGWKK QYVVVSSKKI
1160 1170 1180 1190 1200
LFYNDEQDKE QSNPSMVLDI DKLFHVRPVT QGDVYRAETE EIPKIFQILY
1210 1220 1230 1240 1250
ANEGECRKDV EVEPVQQAEK TNFQNHKGHE FIPTLYHFPA NCEACAKPLW
1260 1270 1280 1290 1300
HVFKPPPALE CRRCHVKCHR DHLDKKEDLI SPCKVSYDVT SARDMLLLAC
1310 1320 1330 1340 1350
SQDEQKKWVT HLVKKIPKNP PSGFVRASPR TLSTRSTANQ SFRKVVKNTS
GKTS
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U42424 mRNA Translation: AAC36189.1 |
RefSeqi | NP_001075836.1, NM_001082367.1 |
Genome annotation databases
GeneIDi | 100009220 |
KEGGi | ocu:100009220 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U42424 mRNA Translation: AAC36189.1 |
RefSeqi | NP_001075836.1, NM_001082367.1 |
3D structure databases
AlphaFoldDBi | O77819 |
SMRi | O77819 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 9986.ENSOCUP00000004197 |
Genome annotation databases
GeneIDi | 100009220 |
KEGGi | ocu:100009220 |
Organism-specific databases
CTDi | 6093 |
Phylogenomic databases
eggNOGi | KOG0612, Eukaryota |
InParanoidi | O77819 |
OrthoDBi | 759391at2759 |
Family and domain databases
CDDi | cd00029, C1, 1 hit cd11639, HR1_ROCK1, 1 hit |
Gene3Di | 2.30.29.30, 1 hit |
InterProi | View protein in InterPro IPR000961, AGC-kinase_C IPR046349, C1-like_sf IPR011072, HR1_rho-bd IPR011009, Kinase-like_dom_sf IPR002219, PE/DAG-bd IPR011993, PH-like_dom_sf IPR001849, PH_domain IPR000719, Prot_kinase_dom IPR017441, Protein_kinase_ATP_BS IPR020684, ROCK1/ROCK2 IPR037310, ROCK1_HR1 IPR015008, ROCK_Rho-bd_dom IPR008271, Ser/Thr_kinase_AS |
Pfami | View protein in Pfam PF00069, Pkinase, 1 hit PF08912, Rho_Binding, 1 hit |
PIRSFi | PIRSF037568, Rho_kinase, 1 hit |
SMARTi | View protein in SMART SM00109, C1, 1 hit SM00233, PH, 1 hit SM00133, S_TK_X, 1 hit SM00220, S_TKc, 1 hit |
SUPFAMi | SSF56112, SSF56112, 1 hit SSF57889, SSF57889, 1 hit |
PROSITEi | View protein in PROSITE PS51285, AGC_KINASE_CTER, 1 hit PS50003, PH_DOMAIN, 1 hit PS00107, PROTEIN_KINASE_ATP, 1 hit PS50011, PROTEIN_KINASE_DOM, 1 hit PS00108, PROTEIN_KINASE_ST, 1 hit PS51860, REM_1, 1 hit PS51859, RHO_BD, 1 hit PS50081, ZF_DAG_PE_2, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | ROCK1_RABIT | |
Accessioni | O77819Primary (citable) accession number: O77819 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | May 24, 2004 |
Last sequence update: | November 1, 1998 | |
Last modified: | May 25, 2022 | |
This is version 159 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families