UniProtKB - O77760 (SOAT1_CHLAE)
Protein
Sterol O-acyltransferase 1
Gene
SOAT1
Organism
Chlorocebus aethiops (Green monkey) (Cercopithecus aethiops)
Status
Functioni
Catalyzes the formation of fatty acid-cholesterol esters, which are less soluble in membranes than cholesterol. Plays a role in lipoprotein assembly and dietary cholesterol absorption. Utilizes oleoyl-CoA ((9Z)-octadecenoyl-CoA) preferentially as susbstrate: shows a higher activity towards an acyl-CoA substrate with a double bond at the delta-9 position (9Z) than towards saturated acyl-CoA or an unsaturated acyl-CoA with a double bond at the delta-7 (7Z) or delta-11 (11Z) positions.By similarity
Catalytic activityi
- EC:2.3.1.26By similarityThis reaction proceeds in the forwardBy similarity direction.
- This reaction proceeds in the forwardBy similarity direction.
- This reaction proceeds in the forwardBy similarity direction.
- This reaction proceeds in the forwardBy similarity direction.
- (9Z,12Z)-octadecadienoyl-CoA + cholesterol = cholesteryl (9Z,12Z)-octadecadienoate + CoABy similarityThis reaction proceeds in the forwardBy similarity direction.
- (5Z,8Z,11Z,14Z)-eicosatetraenoyl-CoA + cholesterol = cholesteryl (5Z,8Z,11Z,14Z)-eicosatetraenoate + CoABy similarityThis reaction proceeds in the forwardBy similarity direction.
- This reaction proceeds in the forwardBy similarity direction.
- This reaction proceeds in the forwardBy similarity direction.
- This reaction proceeds in the forwardBy similarity direction.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 137 | CholesterolBy similarity | 1 | |
Binding sitei | 415 | Acyl-CoABy similarity | 1 | |
Binding sitei | 418 | Acyl-CoABy similarity | 1 | |
Binding sitei | 421 | Acyl-CoABy similarity | 1 | |
Binding sitei | 425 | Acyl-CoABy similarity | 1 | |
Binding sitei | 433 | Acyl-CoABy similarity | 1 | |
Binding sitei | 445 | Acyl-CoABy similarity | 1 | |
Binding sitei | 456 | Acyl-CoABy similarity | 1 | |
Active sitei | 460 | By similarity | 1 |
GO - Molecular functioni
- cholesterol binding Source: UniProtKB
- cholesterol O-acyltransferase activity Source: UniProtKB
- sterol O-acyltransferase activity Source: UniProtKB
GO - Biological processi
- cholesterol esterification Source: UniProtKB
- cholesterol homeostasis Source: InterPro
- cholesterol metabolic process Source: UniProtKB
Keywordsi
Molecular function | Acyltransferase, Transferase |
Biological process | Cholesterol metabolism, Lipid metabolism, Steroid metabolism, Sterol metabolism |
Enzyme and pathway databases
BRENDAi | 2.3.1.26, 175 |
Names & Taxonomyi
Protein namesi | Recommended name: Sterol O-acyltransferase 1 (EC:2.3.1.26By similarity)Alternative name(s): Acyl-coenzyme A:cholesterol acyltransferase 1 Short name: ACAT-1 Cholesterol acyltransferase 1 |
Gene namesi | Name:SOAT1 Synonyms:ACAT1 |
Organismi | Chlorocebus aethiops (Green monkey) (Cercopithecus aethiops) |
Taxonomic identifieri | 9534 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Cercopithecidae › Cercopithecinae › Chlorocebus |
Subcellular locationi
Endoplasmic reticulum
- Endoplasmic reticulum membrane By similarity; Multi-pass membrane protein By similarity
Endoplasmic reticulum
- endoplasmic reticulum membrane Source: UniProtKB-SubCell
Other locations
- integral component of membrane Source: UniProtKB-KW
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 138 | CytoplasmicCuratedAdd BLAST | 138 | |
Transmembranei | 139 – 160 | Helical; Name=1By similarityAdd BLAST | 22 | |
Topological domaini | 161 – 180 | LumenalCuratedAdd BLAST | 20 | |
Transmembranei | 181 – 206 | Helical; Name=2By similarityAdd BLAST | 26 | |
Topological domaini | 207 – 218 | CytoplasmicCuratedAdd BLAST | 12 | |
Transmembranei | 219 – 244 | Helical; Name=3By similarityAdd BLAST | 26 | |
Topological domaini | 245 – 252 | LumenalCurated | 8 | |
Transmembranei | 253 – 276 | Helical; Name=4By similarityAdd BLAST | 24 | |
Topological domaini | 277 – 319 | CytoplasmicCuratedAdd BLAST | 43 | |
Transmembranei | 320 – 352 | Helical; Name=5By similarityAdd BLAST | 33 | |
Topological domaini | 353 – 369 | LumenalCuratedAdd BLAST | 17 | |
Transmembranei | 370 – 395 | Helical; Name=6By similarityAdd BLAST | 26 | |
Topological domaini | 396 – 443 | CytoplasmicCuratedAdd BLAST | 48 | |
Transmembranei | 444 – 468 | Helical; Name=7By similarityAdd BLAST | 25 | |
Topological domaini | 469 – 474 | LumenalCurated | 6 | |
Transmembranei | 475 – 490 | Helical; Name=8By similarityAdd BLAST | 16 | |
Topological domaini | 491 – 496 | CytoplasmicCurated | 6 | |
Transmembranei | 497 – 528 | Helical; Name=9By similarityAdd BLAST | 32 | |
Topological domaini | 529 – 550 | LumenalCuratedAdd BLAST | 22 |
Keywords - Cellular componenti
Endoplasmic reticulum, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000207638 | 1 – 550 | Sterol O-acyltransferase 1Add BLAST | 550 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 1 | N-acetylmethionineBy similarity | 1 | |
Modified residuei | 8 | PhosphoserineBy similarity | 1 | |
Disulfide bondi | 528 ↔ 546 | By similarity |
Keywords - PTMi
Acetylation, Disulfide bond, PhosphoproteinExpressioni
Tissue specificityi
Expressed in most tissues, but most strongly in the adrenal gland. Expressed more strongly in liver Kupffer cells than in hepatocytes.
Interactioni
Subunit structurei
May form homo- or heterodimers.
Interacts with UBIAD1.
By similarityChemistry databases
BindingDBi | O77760 |
Family & Domainsi
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 403 – 409 | FYXDWWN motifBy similarity | 7 |
Domaini
Each protomer consists of 9 transmembrane segments, which enclose a cytosolic tunnel and a transmembrane tunnel that converge at the predicted catalytic site: acyl-CoA enters the active site through the cytosolic tunnel, whereas cholesterol enters from the side through the transmembrane tunnel.By similarity
Sequence similaritiesi
Keywords - Domaini
Transmembrane, Transmembrane helixFamily and domain databases
InterProi | View protein in InterPro IPR004299, MBOAT_fam IPR014371, Oat_ACAT_DAG_ARE IPR030687, Sterol_acyltranf_meta |
PANTHERi | PTHR10408, PTHR10408, 1 hit |
Pfami | View protein in Pfam PF03062, MBOAT, 1 hit |
PIRSFi | PIRSF000439, Oat_ACAT_DAG_ARE, 1 hit PIRSF500230, Sterol_acyltranf_ACAT, 1 hit |
i Sequence
Sequence statusi: Complete.
O77760-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MVGEEKMSLR NRLSKSRENP EEDEDQRKPA KESLEAPSNG RIDIKQLIAK
60 70 80 90 100
KIKLTAEAEE LKPFFMKEVG SHFDDFVTNL IEKSASLDNG GCALTTFSIL
110 120 130 140 150
EGEKNNHRAK DLRAPPEQGK IFIARRSLLD ELLEVDHIRT IYHMFIALLI
160 170 180 190 200
LFILSTLVVD YIDEGRLVLE FSLLSYAFGK FPTVVWTWWI MFLSTFSVPY
210 220 230 240 250
FLFQRWATGY SKSSHPLINS LFHGFLFMVF QIGILGFGPT YVVLAYTLPP
260 270 280 290 300
ASRFIIIFEQ IRFVMKAHSF VRENVPRVLN SAKEKSSTVP IPTVNQYLYF
310 320 330 340 350
LFAPTLIYRD SYPRNPTVRW GYVAMQFAQV FGCFFYVYYI FERLCAPLFR
360 370 380 390 400
NIKQEPFSAR VLVLCVFNSI LPGVLILFLT FFAFLHCWLN AFAEMLRFGD
410 420 430 440 450
RMFYKDWWNS TSYSNYYRTW NVVVHDWLYY YAYKDFLWFF SKRFKSAAML
460 470 480 490 500
AVFAVSAVVH EYALAVCLSF FYPVLFVLFM FFGMAFNFIV NDSRKKPIWN
510 520 530 540 550
VMMWTSLFLG NGVLLCFYSQ EWYARQHCPL KNPTFLDYVR PRSWTCRYVF
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF053336 mRNA Translation: AAC62930.1 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF053336 mRNA Translation: AAC62930.1 |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Chemistry databases
BindingDBi | O77760 |
ChEMBLi | CHEMBL3879850 |
Enzyme and pathway databases
BRENDAi | 2.3.1.26, 175 |
Family and domain databases
InterProi | View protein in InterPro IPR004299, MBOAT_fam IPR014371, Oat_ACAT_DAG_ARE IPR030687, Sterol_acyltranf_meta |
PANTHERi | PTHR10408, PTHR10408, 1 hit |
Pfami | View protein in Pfam PF03062, MBOAT, 1 hit |
PIRSFi | PIRSF000439, Oat_ACAT_DAG_ARE, 1 hit PIRSF500230, Sterol_acyltranf_ACAT, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | SOAT1_CHLAE | |
Accessioni | O77760Primary (citable) accession number: O77760 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 18, 2001 |
Last sequence update: | November 1, 1998 | |
Last modified: | December 2, 2020 | |
This is version 82 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |