UniProtKB - O77628 (FOS_BOVIN)
Protein
Proto-oncogene c-Fos
Gene
FOS
Organism
Bos taurus (Bovine)
Status
Functioni
Nuclear phosphoprotein which forms a tight but non-covalently linked complex with the JUN/AP-1 transcription factor. On TGF-beta activation, forms a multimeric SMAD3/SMAD4/JUN/FOS complex, at the AP1/SMAD-binding site to regulate TGF-beta-mediated signaling. Has a critical function in regulating the development of cells destined to form and maintain the skeleton. It is thought to have an important role in signal transduction, cell proliferation and differentiation (By similarity). In growing cells, activates phospholipid synthesis, possibly by activating CDS1 and PI4K2A. This activity requires Tyr-dephosphorylation and association with the endoplasmic reticulum (By similarity).By similarity
GO - Molecular functioni
- chromatin binding Source: Ensembl
- DNA binding Source: AgBase
- DNA-binding transcription activator activity, RNA polymerase II-specific Source: Ensembl
- DNA-binding transcription factor activity Source: AgBase
- DNA-binding transcription factor activity, RNA polymerase II-specific Source: GO_Central
- double-stranded DNA binding Source: AgBase
- protein heterodimerization activity Source: AgBase
- RNA polymerase II activating transcription factor binding Source: Ensembl
- RNA polymerase II cis-regulatory region sequence-specific DNA binding Source: GO_Central
- RNA polymerase II core promoter sequence-specific DNA binding Source: Ensembl
- R-SMAD binding Source: Ensembl
GO - Biological processi
- cellular response to cadmium ion Source: Ensembl
- cellular response to calcium ion Source: Ensembl
- cellular response to extracellular stimulus Source: Ensembl
- cellular response to reactive oxygen species Source: Ensembl
- nervous system development Source: AgBase
- positive regulation of osteoclast differentiation Source: Ensembl
- regulation of transcription, DNA-templated Source: AgBase
- regulation of transcription by RNA polymerase II Source: GO_Central
- response to drug Source: Ensembl
- response to muscle stretch Source: Ensembl
- skeletal muscle cell differentiation Source: Ensembl
- SMAD protein signal transduction Source: Ensembl
- transforming growth factor beta receptor signaling pathway Source: Ensembl
Keywordsi
Molecular function | DNA-binding |
Enzyme and pathway databases
Reactomei | R-BTA-2559580, Oxidative Stress Induced Senescence R-BTA-2871796, FCERI mediated MAPK activation R-BTA-450341, Activation of the AP-1 family of transcription factors R-BTA-9018519, Estrogen-dependent gene expression |
Names & Taxonomyi
Protein namesi | Recommended name: Proto-oncogene c-FosAlternative name(s): Cellular oncogene fos |
Gene namesi | Name:FOS |
Organismi | Bos taurus (Bovine) |
Taxonomic identifieri | 9913 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Artiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos |
Proteomesi |
|
Organism-specific databases
VGNCi | VGNC:29073, FOS |
Subcellular locationi
Endoplasmic reticulum
- Endoplasmic reticulum By similarity
Cytosol
- cytosol By similarity
Nucleus
- Nucleus PROSITE-ProRule annotation
Note: In quiescent cells, present in very small amounts in the cytosol. Following induction of cell growth, first localizes to the endoplasmic reticulum and only later to the nucleus. Localization at the endoplasmic reticulum requires dephosphorylation at Tyr-10 and Tyr-30 (By similarity).By similarity
Cytosol
- cytosol Source: UniProtKB-SubCell
Endoplasmic reticulum
- endoplasmic reticulum Source: UniProtKB-SubCell
Nucleus
- nucleoplasm Source: Ensembl
- nucleus Source: AgBase
Other locations
- protein-DNA complex Source: Ensembl
- transcription factor AP-1 complex Source: Ensembl
- transcription regulator complex Source: AgBase
Keywords - Cellular componenti
Cytoplasm, Endoplasmic reticulum, NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000076462 | 1 – 380 | Proto-oncogene c-FosAdd BLAST | 380 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 10 | Phosphotyrosine; by SRCBy similarity | 1 | |
Modified residuei | 30 | Phosphotyrosine; by SRCBy similarity | 1 | |
Cross-linki | 113 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity | ||
Cross-linki | 128 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity | ||
Modified residuei | 232 | PhosphothreonineBy similarity | 1 | |
Cross-linki | 265 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO); alternateBy similarity | ||
Cross-linki | 265 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2); alternateBy similarity | ||
Modified residuei | 325 | Phosphothreonine; by MAPK1 and MAPK3By similarity | 1 | |
Modified residuei | 331 | Phosphothreonine; by MAPK1 and MAPK3By similarity | 1 | |
Modified residuei | 362 | Phosphoserine; by MAPK1, MAPK3 and RPS6KA3By similarity | 1 | |
Modified residuei | 374 | Phosphoserine; by MAPK1 and MAPK3By similarity | 1 |
Post-translational modificationi
Phosphorylated in the C-terminal upon stimulation by nerve growth factor (NGF) and epidermal growth factor (EGF). Phosphorylated, in vitro, by MAPK and RSK1. Phosphorylation on both Ser-362 and Ser-374 by MAPK1/2 and RSK1/2 leads to protein stabilization with phosphorylation on Ser-374 being the major site for protein stabilization on NGF stimulation. Phosphorylation on Ser-362 and Ser-374 primes further phosphorylations on Thr-325 and Thr-331 through promoting docking of MAPK to the DEF domain. Phosphorylation on Thr-232, induced by HA-RAS, activates the transcriptional activity and antagonizes sumoylation. Phosphorylation on Ser-362 by RSK2 in osteoblasts contributes to osteoblast transformation (By similarity).By similarity
Constitutively sumoylated with SUMO1, SUMO2 and SUMO3. Desumoylated by SENP2. Sumoylation requires heterodimerization with JUN and is enhanced by mitogen stimulation. Sumoylation inhibits the AP-1 transcriptional activity and is, itself, inhibited by Ras-activated phosphorylation on Thr-232 (By similarity).By similarity
In quiescent cells, the small amount of FOS present is phosphorylated at Tyr-10 and Tyr-30 by SRC. This Tyr-phosphorylated form is cytosolic. In growing cells, dephosphorylated by PTPN2. Dephosphorylation leads to the association with endoplasmic reticulum membranes and activation of phospholipid synthesis (By similarity).By similarity
Keywords - PTMi
Isopeptide bond, Phosphoprotein, Ubl conjugationProteomic databases
PaxDbi | O77628 |
PRIDEi | O77628 |
Expressioni
Gene expression databases
Bgeei | ENSBTAG00000004322, Expressed in spermatid and 19 other tissues |
Interactioni
Subunit structurei
Heterodimer; with JUN (By similarity).
Component of the SMAD3/SMAD4/JUN/FOS complex required for synergistic TGF-beta-mediated transcription at the AP1-binding site.
Interacts with SMAD3; the interaction is weak even on TGF-beta activation.
Interacts with MAFB.
Interacts with DSIPI; this interaction inhibits the binding of active AP1 to its target DNA.
Interacts with MAFB (By similarity).
Interacts with CDS1 and PI4K2A (By similarity).
By similarityGO - Molecular functioni
- protein heterodimerization activity Source: AgBase
- RNA polymerase II activating transcription factor binding Source: Ensembl
- R-SMAD binding Source: Ensembl
Protein-protein interaction databases
STRINGi | 9913.ENSBTAP00000005660 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 137 – 200 | bZIPPROSITE-ProRule annotationAdd BLAST | 64 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 139 – 159 | Basic motif; required for the activation of phospholipid synthesis, but not for CDS1-bindingPROSITE-ProRule annotationAdd BLAST | 21 | |
Regioni | 165 – 193 | Leucine-zipperPROSITE-ProRule annotationAdd BLAST | 29 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG1414, Eukaryota |
GeneTreei | ENSGT00940000159276 |
HOGENOMi | CLU_049742_2_0_1 |
InParanoidi | O77628 |
OMAi | FTYPEAE |
OrthoDBi | 1221590at2759 |
TreeFami | TF326301 |
Family and domain databases
InterProi | View protein in InterPro IPR000837, AP-1 IPR004827, bZIP IPR029816, c-Fos/v-Fos |
PANTHERi | PTHR23351, PTHR23351, 1 hit PTHR23351:SF4, PTHR23351:SF4, 1 hit |
Pfami | View protein in Pfam PF00170, bZIP_1, 1 hit |
PRINTSi | PR00042, LEUZIPPRFOS |
SMARTi | View protein in SMART SM00338, BRLZ, 1 hit |
PROSITEi | View protein in PROSITE PS50217, BZIP, 1 hit PS00036, BZIP_BASIC, 1 hit |
i Sequence
Sequence statusi: Complete.
O77628-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MMFSGFNADY EASSSRCSSA SPAGDSLSYY HSPADSFSSM GSPVNAQDYC
60 70 80 90 100
TDLAVSSANF IPTVTAISTS PDLQWLVQPT LVSSVAPSQT RAPHPYGVPT
110 120 130 140 150
PSAGAYSRAG VMKTMTGGRA QSIGRRGKVE QLSPEEEEKR RIRRERNKMA
160 170 180 190 200
AAKCRNRRRE LTDTLQAETD QLEDEKSALQ TEIANLLKEK EKLEFILAAH
210 220 230 240 250
RPACKIPDDL GFPEEMSVAS LDLSGGLPEA ATPESEEAFT LPLLNDPEPK
260 270 280 290 300
PSVEPVKSVG SMELKAEPFD DYMFPASSRP SGSETARSVP DMDLSGSFYA
310 320 330 340 350
ADWEPLHGGS LGMGPMATEL EPLCTPVVTC TPSCTTYTSS FVFTYPEADS
360 370 380
FPSCAAAHRK GSSSNEPSSD SLSSPTLLAL
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 268 | P → L in AAC21577 (Ref. 4) Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AY322482 mRNA Translation: AAP84343.1 BT029837 mRNA Translation: ABM21549.1 BC118280 mRNA Translation: AAI18281.1 AF069515 mRNA Translation: AAC21577.1 |
RefSeqi | NP_877587.1, NM_182786.2 |
Genome annotation databases
Ensembli | ENSBTAT00000005660; ENSBTAP00000005660; ENSBTAG00000004322 |
GeneIDi | 280795 |
KEGGi | bta:280795 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AY322482 mRNA Translation: AAP84343.1 BT029837 mRNA Translation: ABM21549.1 BC118280 mRNA Translation: AAI18281.1 AF069515 mRNA Translation: AAC21577.1 |
RefSeqi | NP_877587.1, NM_182786.2 |
3D structure databases
SMRi | O77628 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 9913.ENSBTAP00000005660 |
Proteomic databases
PaxDbi | O77628 |
PRIDEi | O77628 |
Genome annotation databases
Ensembli | ENSBTAT00000005660; ENSBTAP00000005660; ENSBTAG00000004322 |
GeneIDi | 280795 |
KEGGi | bta:280795 |
Organism-specific databases
CTDi | 2353 |
VGNCi | VGNC:29073, FOS |
Phylogenomic databases
eggNOGi | KOG1414, Eukaryota |
GeneTreei | ENSGT00940000159276 |
HOGENOMi | CLU_049742_2_0_1 |
InParanoidi | O77628 |
OMAi | FTYPEAE |
OrthoDBi | 1221590at2759 |
TreeFami | TF326301 |
Enzyme and pathway databases
Reactomei | R-BTA-2559580, Oxidative Stress Induced Senescence R-BTA-2871796, FCERI mediated MAPK activation R-BTA-450341, Activation of the AP-1 family of transcription factors R-BTA-9018519, Estrogen-dependent gene expression |
Gene expression databases
Bgeei | ENSBTAG00000004322, Expressed in spermatid and 19 other tissues |
Family and domain databases
InterProi | View protein in InterPro IPR000837, AP-1 IPR004827, bZIP IPR029816, c-Fos/v-Fos |
PANTHERi | PTHR23351, PTHR23351, 1 hit PTHR23351:SF4, PTHR23351:SF4, 1 hit |
Pfami | View protein in Pfam PF00170, bZIP_1, 1 hit |
PRINTSi | PR00042, LEUZIPPRFOS |
SMARTi | View protein in SMART SM00338, BRLZ, 1 hit |
PROSITEi | View protein in PROSITE PS50217, BZIP, 1 hit PS00036, BZIP_BASIC, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | FOS_BOVIN | |
Accessioni | O77628Primary (citable) accession number: O77628 Secondary accession number(s): A1L550, Q17QM0, Q7YRP7 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | May 30, 2000 |
Last sequence update: | September 27, 2005 | |
Last modified: | December 2, 2020 | |
This is version 139 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families