UniProtKB - O77504 (S22A1_RABIT)
Solute carrier family 22 member 1
SLC22A1
Functioni
Translocates a broad array of organic cations with various structures and molecular weights including the model compounds 1-methyl-4-phenylpyridinium (MPP), tetraethylammonium (TEA), N-1-methylnicotinamide (NMN), 4-(4-(dimethylamino)styryl)-N-methylpyridinium (ASP), the endogenous compounds choline, guanidine, histamine, epinephrine, adrenaline, noradrenaline and dopamine, and the drugs quinine, and metformin. The transport of organic cations is inhibited by a broad array of compounds like tetramethylammonium (TMA), cocaine, lidocaine, NMDA receptor antagonists, atropine, prazosin, cimetidine, TEA and NMN, guanidine, cimetidine, choline, procainamide, quinine, tetrabutylammonium, and tetrapentylammonium. Translocates organic cations in an electrogenic and pH-independent manner. Translocates organic cations across the plasma membrane in both directions. Transports the polyamines spermine and spermidine. Transports pramipexole across the basolateral membrane of the proximal tubular epithelial cells. The choline transport is activated by MMTS. Regulated by various intracellular signaling pathways including inhibition by protein kinase A activation, and endogenously activation by the calmodulin complex, the calmodulin-dependent kinase II and LCK tyrosine kinase.
2 PublicationsKineticsi
- KM=23 µM for MPP1 Publication
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sitei | 450 | Involved in affinity and selectivity of cations as well as in translocationBy similarity | 1 |
GO - Molecular functioni
- transmembrane transporter activity Source: InterPro
GO - Biological processi
- ion transport Source: UniProtKB-KW
Keywordsi
Biological process | Ion transport, Transport |
Names & Taxonomyi
Protein namesi | Recommended name: Solute carrier family 22 member 1Alternative name(s): Organic cation transporter 1 rbOCT1 |
Gene namesi | Name:SLC22A1 Synonyms:OCT1 |
Organismi | Oryctolagus cuniculus (Rabbit) |
Taxonomic identifieri | 9986 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Lagomorpha › Leporidae › Oryctolagus |
Proteomesi |
|
Subcellular locationi
Plasma membrane
Plasma Membrane
- basolateral plasma membrane Source: UniProtKB-SubCell
Other locations
- integral component of membrane Source: UniProtKB-KW
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 21 | CytoplasmicSequence analysisAdd BLAST | 21 | |
Transmembranei | 22 – 42 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 43 – 149 | ExtracellularSequence analysisAdd BLAST | 107 | |
Transmembranei | 150 – 170 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 171 – 176 | CytoplasmicSequence analysis | 6 | |
Transmembranei | 177 – 197 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 198 – 206 | ExtracellularSequence analysis | 9 | |
Transmembranei | 207 – 229 | HelicalSequence analysisAdd BLAST | 23 | |
Topological domaini | 230 – 237 | CytoplasmicSequence analysis | 8 | |
Transmembranei | 238 – 258 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 259 – 262 | ExtracellularSequence analysis | 4 | |
Transmembranei | 263 – 283 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 284 – 347 | CytoplasmicSequence analysisAdd BLAST | 64 | |
Transmembranei | 348 – 368 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 369 – 374 | ExtracellularSequence analysis | 6 | |
Transmembranei | 375 – 395 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 396 – 402 | CytoplasmicSequence analysis | 7 | |
Transmembranei | 403 – 423 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 424 – 431 | ExtracellularSequence analysis | 8 | |
Transmembranei | 432 – 452 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 453 – 464 | CytoplasmicSequence analysisAdd BLAST | 12 | |
Transmembranei | 465 – 485 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 486 – 492 | ExtracellularSequence analysis | 7 | |
Transmembranei | 493 – 513 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 514 – 554 | CytoplasmicSequence analysisAdd BLAST | 41 |
Keywords - Cellular componenti
Cell membrane, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000333878 | 1 – 554 | Solute carrier family 22 member 1Add BLAST | 554 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Glycosylationi | 71 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Modified residuei | 333 | PhosphoserineBy similarity | 1 |
Post-translational modificationi
Keywords - PTMi
Glycoprotein, PhosphoproteinExpressioni
Tissue specificityi
Family & Domainsi
Sequence similaritiesi
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG0255, Eukaryota |
InParanoidi | O77504 |
OrthoDBi | 704438at2759 |
TreeFami | TF315847 |
Family and domain databases
Gene3Di | 1.20.1250.20, 1 hit |
InterProi | View protein in InterPro IPR020846, MFS_dom IPR005828, MFS_sugar_transport-like IPR036259, MFS_trans_sf IPR004749, Orgcat_transp/SVOP IPR005829, Sugar_transporter_CS |
Pfami | View protein in Pfam PF00083, Sugar_tr, 1 hit |
SUPFAMi | SSF103473, SSF103473, 1 hit |
TIGRFAMsi | TIGR00898, 2A0119, 1 hit |
PROSITEi | View protein in PROSITE PS50850, MFS, 1 hit PS00216, SUGAR_TRANSPORT_1, 1 hit |
i Sequence
Sequence statusi: Complete.
10 20 30 40 50
MPTVDDVLEQ VGEFGWFQKR TFLFLCLISA ILAPIYLGIV FLGFTPDHRC
60 70 80 90 100
RSPGVDELSQ RCGWSPEEEL NYTVPGLGAT DGAFVRQCMR YEVDWNQSSL
110 120 130 140 150
GCVDPLASLA PNRSHLPLGP CQHGWVYDTP GSSIVTEFNL VCADAWKVDL
160 170 180 190 200
FQSCVNLGFF LGSLGVGYIA DRFGRKLCLL LTTLINAVSG VLTAVAPDYT
210 220 230 240 250
SMLLFRLLQG LVSKGSWMSG YTLITEFVGS GYRRTVAILY QVAFSVGLVA
260 270 280 290 300
LSGVAYAIPN WRWLQLTVSL PTFLCLFYYW CVPESPRWLL SQKRNTDAVK
310 320 330 340 350
IMDNIAQKNG KLPPADLKML SLDEDVTEKL SPSLADLFRT PNLRKHTFIL
360 370 380 390 400
MFLWFTCSVL YQGLILHMGA TGGNVYLDFF YSSLVEFPAA FVILVTIDRV
410 420 430 440 450
GRIYPMAASN LAAGVASVIL IFVPQDLHWL TIVLSCVGRM GATIVLQMIC
460 470 480 490 500
LVNAELYPTF VRNLGVMVCS ALCDVGGIIT PFMVFRLMEV WQPLPLIVFG
510 520 530 540 550
VLGLLAGGMT LLLPETKGVA LPETIEDAEN LRRKAKPKES KIYLQVQTSE
LKGP
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF015958 mRNA Translation: AAC23661.1 |
RefSeqi | NP_001075491.1, NM_001082022.1 |
Genome annotation databases
GeneIDi | 100008659 |
KEGGi | ocu:100008659 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF015958 mRNA Translation: AAC23661.1 |
RefSeqi | NP_001075491.1, NM_001082022.1 |
3D structure databases
AlphaFoldDBi | O77504 |
SMRi | O77504 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 9986.ENSOCUP00000002189 |
Genome annotation databases
GeneIDi | 100008659 |
KEGGi | ocu:100008659 |
Organism-specific databases
CTDi | 6580 |
Phylogenomic databases
eggNOGi | KOG0255, Eukaryota |
InParanoidi | O77504 |
OrthoDBi | 704438at2759 |
TreeFami | TF315847 |
Family and domain databases
Gene3Di | 1.20.1250.20, 1 hit |
InterProi | View protein in InterPro IPR020846, MFS_dom IPR005828, MFS_sugar_transport-like IPR036259, MFS_trans_sf IPR004749, Orgcat_transp/SVOP IPR005829, Sugar_transporter_CS |
Pfami | View protein in Pfam PF00083, Sugar_tr, 1 hit |
SUPFAMi | SSF103473, SSF103473, 1 hit |
TIGRFAMsi | TIGR00898, 2A0119, 1 hit |
PROSITEi | View protein in PROSITE PS50850, MFS, 1 hit PS00216, SUGAR_TRANSPORT_1, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | S22A1_RABIT | |
Accessioni | O77504Primary (citable) accession number: O77504 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | May 20, 2008 |
Last sequence update: | November 1, 1998 | |
Last modified: | May 25, 2022 | |
This is version 107 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families