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Protein

Eukaryotic translation initiation factor 3 subunit E

Gene

eIF3e

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is involved in protein synthesis of a specialized repertoire of mRNAs and, together with other initiation factors, stimulates binding of mRNA and methionyl-tRNAi to the 40S ribosome. The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation (PubMed:18493598). In addition to its role in the eIF-3 complex, also functions in protein ubiquitination and degradation (PubMed:18493598, PubMed:28505193). During mitosis required for regulating mitotic microtubule growth and kinetochore formation, and consequently is required for satisfying the spindle assembly checkpoint (SAC) during metaphase to prevent delays in mitotic progression (PubMed:28505193). This is likely by promoting the ubiquitination and degradation of Klp67A, a kinesin-like protein that suppresses microtubule polymerization at plus ends (PubMed:28505193). Acts in the COP9 signalosome (CSN) mediated regulation of cullin neddylation by promoting Cul1 and Cul3 neddylation and negatively regulating the CSN complex subunit CSN5 (PubMed:18493598).UniRule annotation2 Publications

GO - Molecular functioni

  • translation initiation factor activity Source: FlyBase

GO - Biological processi

  • translational initiation Source: FlyBase

Keywordsi

Molecular functionInitiation factor
Biological processProtein biosynthesis

Enzyme and pathway databases

ReactomeiR-DME-156827 L13a-mediated translational silencing of Ceruloplasmin expression
R-DME-72649 Translation initiation complex formation
R-DME-72689 Formation of a pool of free 40S subunits
R-DME-72695 Formation of the ternary complex, and subsequently, the 43S complex
R-DME-72702 Ribosomal scanning and start codon recognition

Names & Taxonomyi

Protein namesi
Recommended name:
Eukaryotic translation initiation factor 3 subunit EUniRule annotation
Short name:
eIF3eUniRule annotation
Alternative name(s):
Eukaryotic translation initiation factor 3 subunit 6UniRule annotation
Gene namesi
Name:eIF3eUniRule annotation
Synonyms:eIF-3p481 Publication, eIF3-S6UniRule annotation, Int61 PublicationUniRule annotation
ORF Names:CG9677Imported
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraHolometabolaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 3L

Organism-specific databases

FlyBaseiFBgn0025582 eIF3e

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Endoplasmic reticulum, Microsome

Pathology & Biotechi

Disruption phenotypei

Homozygous lethal at the first instar larval stage. Larvae are smaller than wild-type and die 3-4 days after hatching.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001235171 – 435Eukaryotic translation initiation factor 3 subunit EAdd BLAST435

Proteomic databases

PaxDbiO77410
PRIDEiO77410

Expressioni

Tissue specificityi

Expression levels in females and males are relatively similar 10 days after oviposition, however by day 15 expression is higher in gravid females than in males (at protein level).1 Publication

Developmental stagei

Expressed throughout development and in adults (at protein level). Expression peaks in early embryos (2 h after oviposition), then steadily decreases over the embryonic and larval stages (at protein level). Expression increases again 5 days after oviposition and remains stable until day 10 (at protein level).1 Publication

Gene expression databases

BgeeiFBgn0025582
ExpressionAtlasiO77410 baseline and differential
GenevisibleiO77410 DM

Interactioni

Subunit structurei

Component of the eukaryotic translation initiation factor 3 (eIF-3) complex. The eIF-3 complex interacts with pix. Interacts with mxt (PubMed:23716590).UniRule annotation1 Publication

Protein-protein interaction databases

BioGridi65176, 17 interactors
DIPiDIP-19544N
IntActiO77410, 3 interactors
STRINGi7227.FBpp0075104

Structurei

3D structure databases

ProteinModelPortaliO77410
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini219 – 392PCIPROSITE-ProRule annotationAdd BLAST174

Sequence similaritiesi

Belongs to the eIF-3 subunit E family.UniRule annotation

Phylogenomic databases

eggNOGiKOG2758 Eukaryota
ENOG410XQK5 LUCA
GeneTreeiENSGT00390000002661
InParanoidiO77410
KOiK03250
OMAiGFFRTQV
OrthoDBiEOG091G075K
PhylomeDBiO77410

Family and domain databases

HAMAPiMF_03004 eIF3e, 1 hit
InterProiView protein in InterPro
IPR016650 eIF3e
IPR019010 eIF3e_N
IPR000717 PCI_dom
IPR036390 WH_DNA-bd_sf
PANTHERiPTHR10317 PTHR10317, 1 hit
PfamiView protein in Pfam
PF09440 eIF3_N, 1 hit
PF01399 PCI, 1 hit
PIRSFiPIRSF016255 eIF3e_su6, 1 hit
SMARTiView protein in SMART
SM01186 eIF3_N, 1 hit
SM00088 PINT, 1 hit
SUPFAMiSSF46785 SSF46785, 1 hit
PROSITEiView protein in PROSITE
PS50250 PCI, 1 hit

Sequencei

Sequence statusi: Complete.

O77410-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MANFDLTRIN CQFLDRHLTF PLLEFLCGKE IYNQQELLEY ILETVNKTNM
60 70 80 90 100
IDYTMDTRKR LNLSQEMPEE LVQRKAEVLA TLKQLQNEVA PIMKATDILK
110 120 130 140 150
NGESMKDSKT FVNALQKDYN FKVEHLESAY KLAKYLYECG NYQESTSYLY
160 170 180 190 200
FCLIVMSPND KNYLNVLWGK LAAEILTLNW NTALEDLTRL RDYIDNANFS
210 220 230 240 250
TIQALQQRTW LIHWSVLVFF NHPKGRDLII EMFLYKPLYL NAIQTMCPHI
260 270 280 290 300
MRYLATAVVI NRTRRNALKD LIKVIQQESY TYRDPITEFL ECLYVNFDFE
310 320 330 340 350
GARLKLHECQ TVILNDFFIV ACLNEFVEDA RLMIFETFCR IHQCITISML
360 370 380 390 400
ADKLNMKPNE AECWIVNLIR NARLNAKIDS KLGHVVMGTQ PLSPYQQLVE
410 420 430
KIDSLSMRSE HLAGLIERKS KQKQNQESAD SWKYY
Length:435
Mass (Da):51,162
Last modified:November 1, 1998 - v1
Checksum:i6F79D32FBA3EA711
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U89162 mRNA Translation: AAC62307.1
AE014296 Genomic DNA Translation: AAF49412.1
AF132551 mRNA Translation: AAD27850.1
RefSeqiNP_477385.1, NM_058037.5
UniGeneiDm.3105

Genome annotation databases

EnsemblMetazoaiFBtr0075345; FBpp0075104; FBgn0025582
GeneIDi39877
KEGGidme:Dmel_CG9677

Similar proteinsi

Entry informationi

Entry nameiEIF3E_DROME
AccessioniPrimary (citable) accession number: O77410
Secondary accession number(s): Q9VVA2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: November 1, 1998
Last modified: May 23, 2018
This is version 130 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. Translation initiation factors
    List of translation initiation factor entries
  3. SIMILARITY comments
    Index of protein domains and families

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