UniProtKB - O77325 (O77325_PLAF7)
Protein
Pre-mRNA-processing factor 19
Gene
PF3D7_0308600
Organism
Plasmodium falciparum (isolate 3D7)
Status
Functioni
Ubiquitin-protein ligase which is mainly involved pre-mRNA splicing and DNA repair. Required for pre-mRNA splicing as component of the spliceosome.UniRule annotation
Catalytic activityi
- S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.UniRule annotationARBA annotation EC:2.3.2.27
: protein ubiquitination Pathwayi
This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.UniRule annotationView all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.
GO - Molecular functioni
- beta-tubulin binding Source: InterPro
- ubiquitin protein ligase activity Source: InterPro
- ubiquitin-protein transferase activity Source: GO_Central
GO - Biological processi
- DNA repair Source: InterPro
- generation of catalytic spliceosome for first transesterification step Source: GO_Central
- post-chaperonin tubulin folding pathway Source: InterPro
- tubulin complex assembly Source: InterPro
Keywordsi
Molecular function | TransferaseUniRule annotationARBA annotation |
Biological process | DNA damage, DNA repairUniRule annotation, mRNA processing, mRNA splicing, Ubl conjugation pathwayUniRule annotationARBA annotation |
Enzyme and pathway databases
UniPathwayi | UPA00143 |
Names & Taxonomyi
Protein namesi | |
Gene namesi | ORF Names:PF3D7_0308600Imported |
Organismi | Plasmodium falciparum (isolate 3D7)Imported |
Taxonomic identifieri | 36329 [NCBI] |
Taxonomic lineagei | Eukaryota › Sar › Alveolata › Apicomplexa › Aconoidasida › Haemosporida › Plasmodiidae › Plasmodium › Plasmodium (Laverania) › |
Proteomesi |
|
Organism-specific databases
EuPathDBi | PlasmoDB:PF3D7_0308600 |
Subcellular locationi
- Nucleus UniRule annotationARBA annotation
GO - Cellular componenti
- catalytic step 2 spliceosome Source: GO_Central
- nuclear matrix Source: GeneDB
- nucleoplasm Source: UniProtKB-SubCell
- Prp19 complex Source: GO_Central
- U2-type catalytic step 1 spliceosome Source: GO_Central
Keywords - Cellular componenti
NucleusUniRule annotationARBA annotation, SpliceosomeUniRule annotationARBA annotationInteractioni
Subunit structurei
Homotetramer.
UniRule annotationGO - Molecular functioni
- beta-tubulin binding Source: InterPro
Protein-protein interaction databases
IntActi | O77325, 1 interactor |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 1 – 71 | U-boxInterPro annotationAdd BLAST | 71 | |
Domaini | 228 – 532 | WD_REPEATS_REGIONInterPro annotationAdd BLAST | 305 | |
Repeati | 251 – 283 | WDPROSITE-ProRule annotationAdd BLAST | 33 | |
Repeati | 331 – 372 | WDPROSITE-ProRule annotationAdd BLAST | 42 | |
Repeati | 415 – 456 | WDPROSITE-ProRule annotationAdd BLAST | 42 | |
Repeati | 500 – 532 | WDPROSITE-ProRule annotationAdd BLAST | 33 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 290 – 313 | DisorderedSequence analysisAdd BLAST | 24 |
Coiled coil
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Coiled coili | 109 – 143 | Sequence analysisAdd BLAST | 35 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 298 – 313 | PolarSequence analysisAdd BLAST | 16 |
Sequence similaritiesi
Keywords - Domaini
Coiled coilSequence analysis, RepeatARBA annotation, WD repeatPROSITE-ProRule annotationARBA annotationPhylogenomic databases
HOGENOMi | CLU_023894_1_0_1 |
InParanoidi | O77325 |
OMAi | QHWAFSD |
PhylomeDBi | O77325 |
Family and domain databases
Gene3Di | 3.30.40.10, 1 hit |
InterProi | View protein in InterPro IPR020472, G-protein_beta_WD-40_rep IPR013915, Pre-mRNA_splic_Prp19 IPR038959, Prp19 IPR036126, TBCA_sf IPR003613, Ubox_domain IPR001680, WD40_repeat IPR019775, WD40_repeat_CS IPR017986, WD40_repeat_dom IPR036322, WD40_repeat_dom_sf IPR013083, Znf_RING/FYVE/PHD |
PANTHERi | PTHR43995, PTHR43995, 1 hit |
Pfami | View protein in Pfam PF08606, Prp19, 1 hit PF00400, WD40, 3 hits |
PRINTSi | PR00320, GPROTEINBRPT |
SMARTi | View protein in SMART SM00504, Ubox, 1 hit SM00320, WD40, 6 hits |
SUPFAMi | SSF46988, SSF46988, 1 hit SSF50978, SSF50978, 1 hit |
PROSITEi | View protein in PROSITE PS51698, U_BOX, 1 hit PS00678, WD_REPEATS_1, 1 hit PS50082, WD_REPEATS_2, 4 hits PS50294, WD_REPEATS_REGION, 1 hit |
i Sequence
Sequence statusi: Complete.
O77325-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSIICTISGQ TPEEPVISKT GYIFEKRLIE KHIINYGICP VSGEVLTLED
60 70 80 90 100
LYPIKNEKIV KPRPITASSI PGLLSIFQTE WDSIISEMFS LRTHVNDIRN
110 120 130 140 150
ELSHSLYQYD AATRVIAKLL KEKNGYKEEI ENLKKQIFQL KSSNDLDIYE
160 170 180 190 200
IGLNEELLEK MQNVAKDLLI NRKKRKIDNV CSVEQWKDFK NTNEFNIHSS
210 220 230 240 250
TIPGVTCITL DVNKYKYNYN DDHMKHNFFS GGNDGNVYYV SLNDNKILSK
260 270 280 290 300
LQGHLKKVNS IISHPSNFIC ITASNDKTIR IWKGDDNNVH HNDNGDSDSD
310 320 330 340 350
NNNNNNDNDG SHLNNNEYIY DNYQFVSAHV ITKHKDHVTS LALHPLENYF
360 370 380 390 400
ISSSKDSMWI LHDLETAKTI KTSKDNPSSF KHLAIHPDGM MFGIAAQDSN
410 420 430 440 450
IHIYDIKSQE YKATLNGHTK SLNCLSFSEN GYYLASSSKD NTVKLWDLRK
460 470 480 490 500
AQSFQTITLN ETPNFISFDY SGKYLSIAVE NDIQIYNFET KNQANLIKTL
510 520 530
SSHTDIVTQT CFGSTTSYIL SSSMDKTIKL WN
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AL844502 Genomic DNA Translation: CAB11109.1 |
PIRi | T18432 |
RefSeqi | XP_001351160.1, XM_001351124.1 |
Genome annotation databases
EnsemblProtistsi | CAB11109; CAB11109; PF3D7_0308600 |
GeneDBi | PF3D7_0308600.1:pep |
GeneIDi | 814402 |
KEGGi | pfa:PF3D7_0308600 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AL844502 Genomic DNA Translation: CAB11109.1 |
PIRi | T18432 |
RefSeqi | XP_001351160.1, XM_001351124.1 |
3D structure databases
SMRi | O77325 |
ModBasei | Search... |
Protein-protein interaction databases
IntActi | O77325, 1 interactor |
PTM databases
SwissPalmi | O77325 |
Proteomic databases
PRIDEi | O77325 |
Genome annotation databases
EnsemblProtistsi | CAB11109; CAB11109; PF3D7_0308600 |
GeneDBi | PF3D7_0308600.1:pep |
GeneIDi | 814402 |
KEGGi | pfa:PF3D7_0308600 |
Organism-specific databases
EuPathDBi | PlasmoDB:PF3D7_0308600 |
Phylogenomic databases
HOGENOMi | CLU_023894_1_0_1 |
InParanoidi | O77325 |
OMAi | QHWAFSD |
PhylomeDBi | O77325 |
Enzyme and pathway databases
UniPathwayi | UPA00143 |
Family and domain databases
Gene3Di | 3.30.40.10, 1 hit |
InterProi | View protein in InterPro IPR020472, G-protein_beta_WD-40_rep IPR013915, Pre-mRNA_splic_Prp19 IPR038959, Prp19 IPR036126, TBCA_sf IPR003613, Ubox_domain IPR001680, WD40_repeat IPR019775, WD40_repeat_CS IPR017986, WD40_repeat_dom IPR036322, WD40_repeat_dom_sf IPR013083, Znf_RING/FYVE/PHD |
PANTHERi | PTHR43995, PTHR43995, 1 hit |
Pfami | View protein in Pfam PF08606, Prp19, 1 hit PF00400, WD40, 3 hits |
PRINTSi | PR00320, GPROTEINBRPT |
SMARTi | View protein in SMART SM00504, Ubox, 1 hit SM00320, WD40, 6 hits |
SUPFAMi | SSF46988, SSF46988, 1 hit SSF50978, SSF50978, 1 hit |
PROSITEi | View protein in PROSITE PS51698, U_BOX, 1 hit PS00678, WD_REPEATS_1, 1 hit PS50082, WD_REPEATS_2, 4 hits PS50294, WD_REPEATS_REGION, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | O77325_PLAF7 | |
Accessioni | O77325Primary (citable) accession number: O77325 | |
Entry historyi | Integrated into UniProtKB/TrEMBL: | November 1, 1998 |
Last sequence update: | November 1, 1998 | |
Last modified: | December 2, 2020 | |
This is version 147 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Unreviewed (UniProtKB/TrEMBL) |