UniProtKB - O77059 (CRY1_DROME)
Cryptochrome-1
cry
Functioni
Blue light-dependent regulator that is the input of the circadian feedback loop. Has no photolyase activity for cyclobutane pyrimidine dimers or 6-4 photoproducts. Regulation of expression by light suggests a role in photoreception for locomotor activity rhythms. Functions, together with per, as a transcriptional repressor required for the oscillation of peripheral circadian clocks and for the correct specification of clock cells. Genes directly activated by the transcription factors Clock (Clk) and cycle (cyc) are repressed by cry. Necessary for light-dependent magnetosensitivity, an intact circadian system is not required for the magnetoreception mechanism to operate. Required for both the naive and trained responses to magnetic field, consistent with the notion that cry is in the input pathway of magnetic sensing.
9 PublicationsMiscellaneous
Cofactori
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 237 | FAD2 Publications | 1 | |
Binding sitei | 265 | FAD2 Publications | 1 | |
Binding sitei | 267 | FAD; via amide nitrogenBy similarity | 1 | |
Binding sitei | 311 | FAD2 Publications | 1 | |
Binding sitei | 378 | FAD2 Publications | 1 | |
Binding sitei | 416 | FAD2 Publications | 1 | |
Binding sitei | 419 | FAD2 Publications | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 410 – 412 | FAD2 Publications | 3 |
GO - Molecular functioni
- blue light photoreceptor activity Source: UniProtKB
- DNA binding Source: GO_Central
- FAD binding Source: UniProtKB
- flavin adenine dinucleotide binding Source: UniProtKB
- G protein-coupled photoreceptor activity Source: FlyBase
- photoreceptor activity Source: FlyBase
GO - Biological processi
- blue light signaling pathway Source: FlyBase
- cellular response to light stimulus Source: FlyBase
- circadian behavior Source: FlyBase
- circadian regulation of gene expression Source: FlyBase
- circadian rhythm Source: FlyBase
- detection of light stimulus involved in magnetoreception Source: UniProtKB
- entrainment of circadian clock Source: FlyBase
- entrainment of circadian clock by photoperiod Source: FlyBase
- gravitaxis Source: FlyBase
- locomotor rhythm Source: FlyBase
- magnetoreception Source: FlyBase
- negative regulation of transcription, DNA-templated Source: UniProtKB
- phototransduction Source: FlyBase
- protein-chromophore linkage Source: UniProtKB-KW
- regulation of circadian rhythm Source: FlyBase
- regulation of circadian sleep/wake cycle, sleep Source: FlyBase
- response to blue light Source: FlyBase
- response to light stimulus Source: FlyBase
- response to magnetism Source: FlyBase
- UV-A, blue light phototransduction Source: FlyBase
Keywordsi
Molecular function | Photoreceptor protein, Receptor, Repressor |
Biological process | Biological rhythms, Sensory transduction, Transcription, Transcription regulation |
Ligand | Chromophore, FAD, Flavoprotein, Nucleotide-binding |
Enzyme and pathway databases
Reactomei | R-DME-432395, Degradation of TIM R-DME-432553, Phosphorylation of PER and TIM R-DME-538864, Degradation of CRY |
SignaLinki | O77059 |
Names & Taxonomyi
Protein namesi | Recommended name: Cryptochrome-1Short name: DmCRY1Imported Short name: dcryImported Alternative name(s): Blue light photoreceptorImported |
Gene namesi | Name:cryImported ORF Names:CG3772 |
Organismi | Drosophila melanogaster (Fruit fly) |
Taxonomic identifieri | 7227 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Ecdysozoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora › |
Proteomesi |
|
Organism-specific databases
FlyBasei | FBgn0025680, cry |
VEuPathDBi | VectorBase:FBgn0025680 |
Subcellular locationi
Nucleus
- Nucleus 2 Publications
Cytoplasm and Cytosol
- Cytoplasm 2 Publications
Other locations
- perinuclear region 2 Publications
Note: Nuclear translocation initiates after the perception of a light signal. Accumulates in the perinuclear region about one hour before translocation into the nucleus. Translocation occurs through interaction with other Clock proteins such as tim and per.2 Publications
Cytosol
- cytosol Source: FlyBase
Nucleus
- nucleoplasm Source: Reactome
- nucleus Source: FlyBase
Other locations
- cytoplasm Source: FlyBase
- perinuclear region of cytoplasm Source: UniProtKB-SubCell
Keywords - Cellular componenti
Cytoplasm, NucleusPathology & Biotechi
Disruption phenotypei
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 337 | C → A: Accelerates formation and decay of the FAD radical. 1 Publication | 1 | |
Mutagenesisi | 397 | W → F: Abolishes formation of the FAD radical. 1 Publication | 1 | |
Mutagenesisi | 410 | D → N in cryb: Loss of accumulation. 1 Publication | 1 | |
Mutagenesisi | 416 | C → A: Accelerates decay of the FAD radical. 1 Publication | 1 | |
Mutagenesisi | 420 | W → A: Abolishes formation of the FAD radical. 1 Publication | 1 | |
Mutagenesisi | 523 | C → A: Accelerates formation and decay of the FAD radical. 1 Publication | 1 | |
Mutagenesisi | 526 | S → A: Slows down the decay of the FAD radical. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000348597 | 1 – 542 | Cryptochrome-1Add BLAST | 542 |
Proteomic databases
PaxDbi | O77059 |
PRIDEi | O77059 |
Expressioni
Tissue specificityi
Inductioni
Gene expression databases
Bgeei | FBgn0025680, Expressed in adult Malpighian tubule (Drosophila) and 39 other tissues |
Genevisiblei | O77059, DM |
Interactioni
Subunit structurei
Binary interactionsi
O77059
With | #Exp. | IntAct |
---|---|---|
sgg [P18431] | 2 | EBI-94117,EBI-242141 |
Protein-protein interaction databases
BioGRIDi | 67302, 28 interactors |
DIPi | DIP-29424N |
IntActi | O77059, 1 interactor |
STRINGi | 7227.FBpp0083150 |
Structurei
Secondary structure
3D structure databases
AlphaFoldDBi | O77059 |
SMRi | O77059 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 5 – 140 | Photolyase/cryptochrome alpha/betaAdd BLAST | 136 |
Domaini
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG0133, Eukaryota |
HOGENOMi | CLU_010348_3_4_1 |
InParanoidi | O77059 |
OMAi | IWFRHGL |
OrthoDBi | 378952at2759 |
PhylomeDBi | O77059 |
Family and domain databases
Gene3Di | 3.40.50.620, 1 hit |
IDEALi | IID50267 |
InterProi | View protein in InterPro IPR036134, Crypto/Photolyase_FAD-like_sf IPR036155, Crypto/Photolyase_N_sf IPR005101, Cryptochr/Photolyase_FAD-bd IPR002081, Cryptochrome/DNA_photolyase_1 IPR006050, DNA_photolyase_N IPR014729, Rossmann-like_a/b/a_fold |
PANTHERi | PTHR11455, PTHR11455, 1 hit |
Pfami | View protein in Pfam PF00875, DNA_photolyase, 1 hit PF03441, FAD_binding_7, 1 hit |
PRINTSi | PR00147, DNAPHOTLYASE |
SUPFAMi | SSF48173, SSF48173, 1 hit SSF52425, SSF52425, 1 hit |
PROSITEi | View protein in PROSITE PS51645, PHR_CRY_ALPHA_BETA, 1 hit |
i Sequence
Sequence statusi: Complete.
10 20 30 40 50
MATRGANVIW FRHGLRLHDN PALLAALADK DQGIALIPVF IFDGESAGTK
60 70 80 90 100
NVGYNRMRFL LDSLQDIDDQ LQAATDGRGR LLVFEGEPAY IFRRLHEQVR
110 120 130 140 150
LHRICIEQDC EPIWNERDES IRSLCRELNI DFVEKVSHTL WDPQLVIETN
160 170 180 190 200
GGIPPLTYQM FLHTVQIIGL PPRPTADARL EDATFVELDP EFCRSLKLFE
210 220 230 240 250
QLPTPEHFNV YGDNMGFLAK INWRGGETQA LLLLDERLKV EQHAFERGFY
260 270 280 290 300
LPNQALPNIH DSPKSMSAHL RFGCLSVRRF YWSVHDLFKN VQLRACVRGV
310 320 330 340 350
QMTGGAHITG QLIWREYFYT MSVNNPNYDR MEGNDICLSI PWAKPNENLL
360 370 380 390 400
QSWRLGQTGF PLIDGAMRQL LAEGWLHHTL RNTVATFLTR GGLWQSWEHG
410 420 430 440 450
LQHFLKYLLD ADWSVCAGNW MWVSSSAFER LLDSSLVTCP VALAKRLDPD
460 470 480 490 500
GTYIKQYVPE LMNVPKEFVH EPWRMSAEQQ EQYECLIGVH YPERIIDLSM
510 520 530 540
AVKRNMLAMK SLRNSLITPP PHCRPSNEEE VRQFFWLADV VV
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 232 | L → H in BAA35000 (Ref. 3) Curated | 1 | |
Sequence conflicti | 335 | D → E in BAA35000 (Ref. 3) Curated | 1 | |
Sequence conflicti | 348 | N → D in BAA35000 (Ref. 3) Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF099734 mRNA Translation: AAC83828.1 AB018400 mRNA Translation: BAA33787.1 AB019389 mRNA Translation: BAA35000.1 AE014297 Genomic DNA Translation: AAF55649.1 AY051514 mRNA Translation: AAK92938.1 |
RefSeqi | NP_732407.1, NM_169852.2 |
Genome annotation databases
EnsemblMetazoai | FBtr0083736; FBpp0083150; FBgn0025680 |
GeneIDi | 42305 |
KEGGi | dme:Dmel_CG3772 |
UCSCi | CG3772-RA, d. melanogaster |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF099734 mRNA Translation: AAC83828.1 AB018400 mRNA Translation: BAA33787.1 AB019389 mRNA Translation: BAA35000.1 AE014297 Genomic DNA Translation: AAF55649.1 AY051514 mRNA Translation: AAK92938.1 |
RefSeqi | NP_732407.1, NM_169852.2 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
4GU5 | X-ray | 2.30 | A/B | 1-539 | [»] | |
4JZY | X-ray | 2.34 | A/B | 1-540 | [»] | |
4K03 | X-ray | 3.20 | A/B | 1-542 | [»] | |
6WTB | X-ray | 2.58 | A/B | 1-539 | [»] | |
AlphaFoldDBi | O77059 | |||||
SMRi | O77059 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 67302, 28 interactors |
DIPi | DIP-29424N |
IntActi | O77059, 1 interactor |
STRINGi | 7227.FBpp0083150 |
Proteomic databases
PaxDbi | O77059 |
PRIDEi | O77059 |
Protocols and materials databases
DNASUi | 42305 |
Genome annotation databases
EnsemblMetazoai | FBtr0083736; FBpp0083150; FBgn0025680 |
GeneIDi | 42305 |
KEGGi | dme:Dmel_CG3772 |
UCSCi | CG3772-RA, d. melanogaster |
Organism-specific databases
CTDi | 42305 |
FlyBasei | FBgn0025680, cry |
VEuPathDBi | VectorBase:FBgn0025680 |
Phylogenomic databases
eggNOGi | KOG0133, Eukaryota |
HOGENOMi | CLU_010348_3_4_1 |
InParanoidi | O77059 |
OMAi | IWFRHGL |
OrthoDBi | 378952at2759 |
PhylomeDBi | O77059 |
Enzyme and pathway databases
Reactomei | R-DME-432395, Degradation of TIM R-DME-432553, Phosphorylation of PER and TIM R-DME-538864, Degradation of CRY |
SignaLinki | O77059 |
Miscellaneous databases
BioGRID-ORCSi | 42305, 0 hits in 3 CRISPR screens |
ChiTaRSi | cry, fly |
GenomeRNAii | 42305 |
PROi | PR:O77059 |
Gene expression databases
Bgeei | FBgn0025680, Expressed in adult Malpighian tubule (Drosophila) and 39 other tissues |
Genevisiblei | O77059, DM |
Family and domain databases
Gene3Di | 3.40.50.620, 1 hit |
IDEALi | IID50267 |
InterProi | View protein in InterPro IPR036134, Crypto/Photolyase_FAD-like_sf IPR036155, Crypto/Photolyase_N_sf IPR005101, Cryptochr/Photolyase_FAD-bd IPR002081, Cryptochrome/DNA_photolyase_1 IPR006050, DNA_photolyase_N IPR014729, Rossmann-like_a/b/a_fold |
PANTHERi | PTHR11455, PTHR11455, 1 hit |
Pfami | View protein in Pfam PF00875, DNA_photolyase, 1 hit PF03441, FAD_binding_7, 1 hit |
PRINTSi | PR00147, DNAPHOTLYASE |
SUPFAMi | SSF48173, SSF48173, 1 hit SSF52425, SSF52425, 1 hit |
PROSITEi | View protein in PROSITE PS51645, PHR_CRY_ALPHA_BETA, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | CRY1_DROME | |
Accessioni | O77059Primary (citable) accession number: O77059 Secondary accession number(s): Q9TYA0 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | September 2, 2008 |
Last sequence update: | November 1, 1998 | |
Last modified: | May 25, 2022 | |
This is version 164 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Drosophila annotation project |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Drosophila
Drosophila: entries, gene names and cross-references to FlyBase - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families