UniProtKB - O76464 (NFT1_DROME)
Protein
Nitrilase and fragile histidine triad fusion protein NitFhit
Gene
NitFhit
Organism
Drosophila melanogaster (Fruit fly)
Status
Functioni
Cleaves A-5'-PPP-5'A to yield AMP and ADP.1 Publication
Catalytic activityi
- EC:3.6.1.291 Publication
Cofactori
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 72 | PROSITE-ProRule annotation | 1 | |
Active sitei | 142 | PROSITE-ProRule annotation | 1 | |
Active sitei | 183 | PROSITE-ProRule annotation | 1 | |
Active sitei | 409 | Tele-AMP-histidine intermediatePROSITE-ProRule annotationBy similarity | 1 |
GO - Molecular functioni
- bis(5'-adenosyl)-triphosphatase activity Source: UniProtKB
- hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds Source: UniProtKB
- nucleotide binding Source: UniProtKB-KW
GO - Biological processi
- nucleobase-containing compound metabolic process Source: UniProtKB
Keywordsi
Molecular function | Hydrolase, Multifunctional enzyme |
Ligand | Manganese, Nucleotide-binding |
Names & Taxonomyi
Protein namesi | Recommended name: Nitrilase and fragile histidine triad fusion protein NitFhitAlternative name(s): NFT-1 protein Including the following 2 domains: |
Gene namesi | Name:NitFhitImported ORF Names:CG7067 |
Organismi | Drosophila melanogaster (Fruit fly) |
Taxonomic identifieri | 7227 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Ecdysozoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Holometabola › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora › |
Proteomesi |
|
Organism-specific databases
FlyBasei | FBgn0024945 NitFhit |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000109792 | 1 – 460 | Nitrilase and fragile histidine triad fusion protein NitFhitAdd BLAST | 460 |
Proteomic databases
PaxDbi | O76464 |
PRIDEi | O76464 |
Expressioni
Developmental stagei
Expressed in embryo and adult.1 Publication
Gene expression databases
Bgeei | FBgn0024945 Expressed in 33 organ(s), highest expression level in adult Malpighian tubule (Drosophila) |
Genevisiblei | O76464 DM |
Interactioni
Subunit structurei
Homotetramer.
By similarityProtein-protein interaction databases
BioGridi | 63593, 2 interactors |
IntActi | O76464, 1 interactor |
STRINGi | 7227.FBpp0072385 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 33 – 279 | CN hydrolasePROSITE-ProRule annotationAdd BLAST | 247 | |
Domaini | 315 – 422 | HITPROSITE-ProRule annotationAdd BLAST | 108 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 407 – 411 | Histidine triad motif | 5 |
Sequence similaritiesi
In the N-terminal section; belongs to the UPF0012 family.Sequence analysis
Phylogenomic databases
eggNOGi | KOG0807 Eukaryota KOG3379 Eukaryota COG0388 LUCA COG0537 LUCA |
GeneTreei | ENSGT00550000075099 |
InParanoidi | O76464 |
KOi | K11206 |
OMAi | CFEHRRN |
OrthoDBi | 1154369at2759 |
PhylomeDBi | O76464 |
Family and domain databases
CDDi | cd01275 FHIT, 1 hit |
Gene3Di | 3.30.428.10, 1 hit 3.60.110.10, 1 hit |
InterProi | View protein in InterPro IPR003010 C-N_Hydrolase IPR036526 C-N_Hydrolase_sf IPR039383 FHIT IPR019808 Histidine_triad_CS IPR001310 Histidine_triad_HIT IPR011146 HIT-like IPR036265 HIT-like_sf |
Pfami | View protein in Pfam PF00795 CN_hydrolase, 1 hit PF01230 HIT, 1 hit |
SUPFAMi | SSF54197 SSF54197, 1 hit SSF56317 SSF56317, 1 hit |
PROSITEi | View protein in PROSITE PS50263 CN_HYDROLASE, 1 hit PS00892 HIT_1, 1 hit PS51084 HIT_2, 1 hit |
i Sequence
Sequence statusi: Complete.
O76464-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSTLVNTTRR SIVIAIHQQL RRMSVQKRKD QSATIAVGQM RSTSDKAANL
60 70 80 90 100
SQVIELVDRA KSQNACMLFL PECCDFVGES RTQTIELSEG LDGELMAQYR
110 120 130 140 150
ELAKCNKIWI SLGGVHERND QKIFNAHVLL NEKGELAAVY RKLHMFDVTT
160 170 180 190 200
KEVRLRESDT VTPGYCLERP VSTPVGQIGL QICYDLRFAE PAVLLRKLGA
210 220 230 240 250
NLLTYPSAFT YATGKAHWEI LLRARAIETQ CFVVAAAQIG WHNQKRQSWG
260 270 280 290 300
HSMIVSPWGN VLADCSEQEL DIGTAEVDLS VLQSLYQTMP CFEHRRNDIY
310 320 330 340 350
ALTAYNLRSK EPTQDRPFAT NIVDKRTIFY ESEHCFAFTN LRCVVKGHVL
360 370 380 390 400
VSTKRVTPRL CGLDCAEMAD MFTTVCLVQR LLEKIYQTTS ATVTVQDGAQ
410 420 430 440 450
AGQTVPHVHF HIMPRRLGDF GHNDQIYVKL DERAEEKPPR TIEERIEEAQ
460
IYRKFLTDIS
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF069989 mRNA Translation: AAC39137.1 AE014296 Genomic DNA Translation: AAF47347.1 AY089221 mRNA Translation: AAL89959.1 |
RefSeqi | NP_525122.1, NM_080383.4 |
Genome annotation databases
EnsemblMetazoai | FBtr0072483; FBpp0072385; FBgn0024945 |
GeneIDi | 38029 |
KEGGi | dme:Dmel_CG7067 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF069989 mRNA Translation: AAC39137.1 AE014296 Genomic DNA Translation: AAF47347.1 AY089221 mRNA Translation: AAL89959.1 |
RefSeqi | NP_525122.1, NM_080383.4 |
3D structure databases
SMRi | O76464 |
ModBasei | Search... |
Protein-protein interaction databases
BioGridi | 63593, 2 interactors |
IntActi | O76464, 1 interactor |
STRINGi | 7227.FBpp0072385 |
Proteomic databases
PaxDbi | O76464 |
PRIDEi | O76464 |
Genome annotation databases
EnsemblMetazoai | FBtr0072483; FBpp0072385; FBgn0024945 |
GeneIDi | 38029 |
KEGGi | dme:Dmel_CG7067 |
Organism-specific databases
CTDi | 38029 |
FlyBasei | FBgn0024945 NitFhit |
Phylogenomic databases
eggNOGi | KOG0807 Eukaryota KOG3379 Eukaryota COG0388 LUCA COG0537 LUCA |
GeneTreei | ENSGT00550000075099 |
InParanoidi | O76464 |
KOi | K11206 |
OMAi | CFEHRRN |
OrthoDBi | 1154369at2759 |
PhylomeDBi | O76464 |
Miscellaneous databases
GenomeRNAii | 38029 |
PROi | PR:O76464 |
Gene expression databases
Bgeei | FBgn0024945 Expressed in 33 organ(s), highest expression level in adult Malpighian tubule (Drosophila) |
Genevisiblei | O76464 DM |
Family and domain databases
CDDi | cd01275 FHIT, 1 hit |
Gene3Di | 3.30.428.10, 1 hit 3.60.110.10, 1 hit |
InterProi | View protein in InterPro IPR003010 C-N_Hydrolase IPR036526 C-N_Hydrolase_sf IPR039383 FHIT IPR019808 Histidine_triad_CS IPR001310 Histidine_triad_HIT IPR011146 HIT-like IPR036265 HIT-like_sf |
Pfami | View protein in Pfam PF00795 CN_hydrolase, 1 hit PF01230 HIT, 1 hit |
SUPFAMi | SSF54197 SSF54197, 1 hit SSF56317 SSF56317, 1 hit |
PROSITEi | View protein in PROSITE PS50263 CN_HYDROLASE, 1 hit PS00892 HIT_1, 1 hit PS51084 HIT_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | NFT1_DROME | |
Accessioni | O76464Primary (citable) accession number: O76464 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | September 13, 2004 |
Last sequence update: | November 1, 1998 | |
Last modified: | October 16, 2019 | |
This is version 129 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Drosophila annotation project |
Miscellaneousi
Keywords - Technical termi
Complete proteome, Reference proteomeDocuments
- Uncharacterized protein families (UPF)
List of uncharacterized protein family (UPF) entries - SIMILARITY comments
Index of protein domains and families - Drosophila
Drosophila: entries, gene names and cross-references to FlyBase