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Entry version 165 (16 Oct 2019)
Sequence version 2 (01 Nov 1999)
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Protein

cGMP-dependent protein kinase egl-4

Gene

egl-4

Organism
Caenorhabditis elegans
Status
Reviewed-Annotation score:

Annotation score:5 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the ‘correct annotation’ for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
-Experimental evidence at protein leveli <p>This indicates the type of evidence that supports the existence of the protein. Note that the ‘protein existence’ evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Promotes chemoreceptor gene expression in response to increased cGMP levels by antagonizing the gene repression functions of the class II HDAC hda-4 and the mef-2 transcription factor (PubMed:18832350). Regulates gene expression via recruitment of a histone deacetylase complex containing hda-2, saeg-1 and saeg-2 (PubMed:21573134). Represses body size and lifespan through the dbl-1 and insulin pathways, respectively (PubMed:12571101, PubMed:15330854, PubMed:26434723). May also signal through daf-3 and/or daf-5. Role in egg-laying, dauer formation and motility (PubMed:12571101, PubMed:11181837, PubMed:21573134). Regulates behavioral responses to various chemosensory stimuli in sensory neurons (PubMed:10978280, PubMed:22319638, PubMed:23874221, PubMed:26434723). Required for the initiation of long term adaptation to prolonged odor exposure which results in a decrease in odor seeking behavior (PubMed:12495623, PubMed:20220099, PubMed:26434723). May regulate this process by phosphorylating tax-2, a subunit of cyclic nucleotide-gated channel tax-2/tax-4 (PubMed:12495623). In ASH sensory neurons, negatively regulates avoidance behavior to some bitter tastants, such as quinine, probably by phosphorylating rgs-2 and rgs-3 which are 2 regulator of G-protein signaling proteins (PubMed:23874221). In AWB sensory neurons, involved in avoidance behavior to some repellent odors (PubMed:23954825). In ASE left (ASEL) sensory neuron, involved in the sensing of environmental alkalinity downstream of receptor-type guanylate cyclase gcy-14 (PubMed:23664973). In sensory neurons, involved in the signaling pathway downstream of insulin, TGF-beta and receptor-type guanylate cyclase responsible for inducing quiescence after food intake (PubMed:18316030). Might play a role in aversive olfactory learning in AWC neurons when an odor is associated with food deprivation, depending on the ins-1/age-1 signal from the AIA to the AWC neurons (PubMed:27383131). Probably by regulating neuronal transmission downstream of lin-3 and receptor lin-23 and phospholipase plc-3 in ALA neurons, involved in the decrease in locomotion during the quiescent state that precedes each larval molt (PubMed:17891142).1 Publication15 Publications

<p>This subsection of the <a href="http://www.uniprot.org/help/function_section">Function</a> section describes the catalytic activity of an enzyme, i.e. a chemical reaction that the enzyme catalyzes.<p><a href='/help/catalytic_activity' target='_top'>More...</a></p>Catalytic activityi

<p>This subsection of the ‘Function’ section provides information relevant to cofactors. A cofactor is any non-protein substance required for a protein to be catalytically active. Some cofactors are inorganic, such as the metal atoms zinc, iron, and copper in various oxidation states. Others, such as most vitamins, are organic.<p><a href='/help/cofactor' target='_top'>More...</a></p>Cofactori

Mg2+2 Publications

<p>This subsection of the <a href="http://www.uniprot.org/help/function_section">Function</a> section describes regulatory mechanisms for enzymes, transporters or microbial transcription factors, and reports the components which regulate (by activation or inhibition) the reaction.<p><a href='/help/activity_regulation' target='_top'>More...</a></p>Activity regulationi

Binding of cGMP results in enzyme activation.2 Publications

<p>This subsection of the ‘Function’ section describes biophysical and chemical properties, such as maximal absorption, kinetic parameters, pH dependence, redox potentials and temperature dependence.<p><a href='/help/biophysicochemical_properties' target='_top'>More...</a></p>Kineticsi

    1. Vmax=2.1 µmol/min/mg enzyme towards cGMP (Isoform c at pH 6.8 and 30 degrees Celsius)1 Publication

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    <p>This subsection of the <a href="http://www.uniprot.org/help/function_section">Function</a> section describes the interaction between a single amino acid and another chemical entity. Priority is given to the annotation of physiological ligands.<p><a href='/help/binding' target='_top'>More...</a></p>Binding sitei380cGMP 2By similarity1
    Binding sitei434cAMP or cGMP 2By similarity1
    Binding sitei499ATPPROSITE-ProRule annotationBy similarity1
    <p>This subsection of the <a href="http://www.uniprot.org/help/function_section">Function</a> section is used for enzymes and indicates the residues directly involved in catalysis.<p><a href='/help/act_site' target='_top'>More...</a></p>Active sitei593Proton acceptorPROSITE-ProRule annotationBy similarity1

    Regions

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    <p>This subsection of the <a href="http://www.uniprot.org/help/function_section">Function</a> section describes a region in the protein which binds nucleotide phosphates. It always involves more than one amino acid and includes all residues involved in nucleotide-binding.<p><a href='/help/np_bind' target='_top'>More...</a></p>Nucleotide bindingi265 – 268cAMP or cGMP 1By similarity4
    Nucleotide bindingi275 – 276cAMP or cGMP 1By similarity2
    Nucleotide bindingi389 – 392cAMP or cGMP 2By similarity4
    Nucleotide bindingi399 – 400cAMP or cGMP 2By similarity2
    Nucleotide bindingi475 – 483ATPPROSITE-ProRule annotationBy similarity9

    <p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

    GO - Biological processi

    <p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

    Molecular functionDevelopmental protein, Kinase, Serine/threonine-protein kinase, Transferase
    Biological processChemotaxis
    LigandATP-binding, cGMP, cGMP-binding, Magnesium, Metal-binding, Nucleotide-binding

    Enzyme and pathway databases

    Reactome - a knowledgebase of biological pathways and processes

    More...
    Reactomei
    R-CEL-1474151 Tetrahydrobiopterin (BH4) synthesis, recycling, salvage and regulation
    R-CEL-392517 Rap1 signalling

    SABIO-RK: Biochemical Reaction Kinetics Database

    More...
    SABIO-RKi
    O76360

    SignaLink: a signaling pathway resource with multi-layered regulatory networks

    More...
    SignaLinki
    O76360

    <p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

    <p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
    Recommended name:
    cGMP-dependent protein kinase egl-42 Publications (EC:2.7.11.123 Publications)
    Alternative name(s):
    Egg-laying defective protein 4
    <p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: ‘Name’, ‘Synonyms’, ‘Ordered locus names’ and ‘ORF names’.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
    Name:egl-4Imported
    Synonyms:cgk-11 Publication, odr-91 Publication
    ORF Names:F55A8.2Imported
    <p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiCaenorhabditis elegans
    <p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the ‘taxonomic identifier’ or ‘taxid’.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri6239 [NCBI]
    <p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditinaRhabditomorphaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
    <p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section is present for entries that are part of a <a href="http://www.uniprot.org/proteomes">proteome</a>, i.e. of a set of proteins thought to be expressed by organisms whose genomes have been completely sequenced.<p><a href='/help/proteomes_manual' target='_top'>More...</a></p>Proteomesi
    • UP000001940 <p>A UniProt <a href="http://www.uniprot.org/manual/proteomes_manual">proteome</a> can consist of several components. <br></br>The component name refers to the genomic component encoding a set of proteins.<p><a href='/help/proteome_component' target='_top'>More...</a></p> Componenti: Chromosome IV

    Organism-specific databases

    WormBase

    More...
    WormBasei
    F55A8.2a ; CE19897 ; WBGene00001173 ; egl-4
    F55A8.2b ; CE19898 ; WBGene00001173 ; egl-4
    F55A8.2c ; CE31541 ; WBGene00001173 ; egl-4
    F55A8.2d ; CE37718 ; WBGene00001173 ; egl-4
    F55A8.2e ; CE37241 ; WBGene00001173 ; egl-4
    F55A8.2f ; CE37242 ; WBGene00001173 ; egl-4

    <p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

    Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte & Seán O’Donoghue; Source: COMPARTMENTS

    Keywords - Cellular componenti

    Cytoplasm, Nucleus

    <p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

    <p>This subsection of the ‘Pathology and Biotech’ section describes the in vivo effects caused by ablation of the gene (or one or more transcripts) coding for the protein described in the entry. This includes gene knockout and knockdown, provided experiments have been performed in the context of a whole organism or a specific tissue, and not at the single-cell level.<p><a href='/help/disruption_phenotype' target='_top'>More...</a></p>Disruption phenotypei

    Increased size and extended lifespan (PubMed:11181837, PubMed:12571101, PubMed:18832350, PubMed:26434723). Defects in chemosensory behavior, egg-laying, synaptic transmission, and dauer formation (PubMed:11181837, PubMed:12571101, PubMed:18832350, PubMed:26434723). Impaired movement of adult animals in response to sensory stimuli (PubMed:10978280). Loss of adaptive behavior to long lasting exposure to attractive odorants (PubMed:20220099). Normal initial chemotaxis response during a first and short exposure to attractive odorants (PubMed:20220099). Loss of chemotaxis response to the repellent odor 2-nonanone (PubMed:23954825). Defective chemotaxis in response to diacetyl and isoamylalcohol (PubMed:26434723). High sensitivity to benzaldehyde, which is retained following pre-exposure in contrast to wild-type animals (PubMed:26434723). Increased basal cGMP levels (PubMed:24015261).8 Publications

    Mutagenesis

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    <p>This subsection of the <a href="http://www.uniprot.org/manual/pathology_and_biotech_section">'Pathology and Biotech'</a> section describes the effect of the experimental mutation of one or more amino acid(s) on the biological properties of the protein.<p><a href='/help/mutagen' target='_top'>More...</a></p>Mutagenesisi162K → N: Constitutively active. Undergoes autophosphorylation in vitro. 1 Publication1
    Mutagenesisi276T → A: Loss of nuclear translocation mediated by cGMP. Loss of nuclear translocation upon prolonged exposure to attractive odorants. 2 Publications1
    Mutagenesisi362G → R: Loss of body size, reduced locomotion in the presence of food, a pale intestine, increased intestinal fat storage, and a decreased propensity to form dauer larvae. 1 Publication1
    Mutagenesisi497A → T: No obvious phenotype. May cause partial loss of kinase activity. 1 Publication1
    Mutagenesisi499K → A: Loss of kinase activity. 1 Publication1
    Mutagenesisi502K → E: Loss of adaptive behavior upon prolonged exposure to attractive odorants. 1 Publication1
    Mutagenesisi611D → N: Loss of nuclear translocation upon prolonged exposure to attractive odorants. 1 Publication1
    Mutagenesisi682G → R: No obvious phenotype. May cause partial loss of kinase activity. 1 Publication1

    <p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    <p>This subsection of the ‘PTM / Processing’ section describes the extent of a polypeptide chain in the mature protein following processing.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_00003904911 – 780cGMP-dependent protein kinase egl-4Add BLAST780

    <p>This subsection of the <a href="http://www.uniprot.org/help/ptm_processing_section">PTM/processing</a> section describes post-translational modifications (PTMs). This subsection <strong>complements</strong> the information provided at the sequence level or describes modifications for which <strong>position-specific data is not yet available</strong>.<p><a href='/help/post-translational_modification' target='_top'>More...</a></p>Post-translational modificationi

    Autophosphorylated.1 Publication

    Proteomic databases

    Encyclopedia of Proteome Dynamics

    More...
    EPDi
    O76360

    PaxDb, a database of protein abundance averages across all three domains of life

    More...
    PaxDbi
    O76360

    PeptideAtlas

    More...
    PeptideAtlasi
    O76360

    PRoteomics IDEntifications database

    More...
    PRIDEi
    O76360

    <p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

    <p>This subsection of the ‘Expression’ section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms. By default, the information is derived from experiments at the mRNA level, unless specified ‘at protein level’. <br></br>Examples: <a href="http://www.uniprot.org/uniprot/P92958#expression">P92958</a>, <a href="http://www.uniprot.org/uniprot/Q8TDN4#expression">Q8TDN4</a>, <a href="http://www.uniprot.org/uniprot/O14734#expression">O14734</a><p><a href='/help/tissue_specificity' target='_top'>More...</a></p>Tissue specificityi

    Expressed in AWC sensory neurons (at protein level) (PubMed:20220099). Mainly expressed in head neurons, hypodermis, intestine and body wall muscles. L2 and L3 larvae show extensive expression, lower levels are observed in L4 larvae, later embryos and adults. Isoform c is expressed in a subset of neurons in the head, nerve ring, and ventral nerve cord including some motor neurons, also in several neurons in the tail, the pharyngeal marginal cells, body muscle, intestine, vulval muscles, and spermatheca (PubMed:11181837, PubMed:12571101, PubMed:15330854).4 Publications

    Gene expression databases

    Bgee dataBase for Gene Expression Evolution

    More...
    Bgeei
    WBGene00001173 Expressed in 5 organ(s), highest expression level in adult organism

    ExpressionAtlas, Differential and Baseline Expression

    More...
    ExpressionAtlasi
    O76360 baseline and differential

    <p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

    <p>This subsection of the <a href="http://www.uniprot.org/help/interaction_section">'Interaction'</a> section provides information about the protein quaternary structure and interaction(s) with other proteins or protein complexes (with the exception of physiological receptor-ligand interactions which are annotated in the <a href="http://www.uniprot.org/help/function_section">'Function'</a> section).<p><a href='/help/subunit_structure' target='_top'>More...</a></p>Subunit structurei

    When phosphorylated, interacts with saeg-2. May interact with saeg-1.

    1 Publication

    Protein-protein interaction databases

    STRING: functional protein association networks

    More...
    STRINGi
    6239.F55A8.2h.1

    <p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

    3D structure databases

    SWISS-MODEL Repository - a database of annotated 3D protein structure models

    More...
    SMRi
    O76360

    Database of comparative protein structure models

    More...
    ModBasei
    Search...

    <p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    <p>This subsection of the <a href="http://www.uniprot.org/help/family_and_domains_section">Family and Domains</a> section describes the position and type of a domain, which is defined as a specific combination of secondary structures organized into a characteristic three-dimensional structure or fold.<p><a href='/help/domain' target='_top'>More...</a></p>Domaini469 – 729Protein kinasePROSITE-ProRule annotationAdd BLAST261
    Domaini730 – 780AGC-kinase C-terminalSequence analysisAdd BLAST51

    Coiled coil

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    <p>This subsection of the ‘Family and domains’ section denotes the positions of regions of coiled coil within the protein.<p><a href='/help/coiled' target='_top'>More...</a></p>Coiled coili61 – 127Sequence analysisAdd BLAST67

    Motif

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    <p>This subsection of the ‘Family and Domains’ section describes a short (usually not more than 20 amino acids) conserved sequence motif of biological significance.<p><a href='/help/motif' target='_top'>More...</a></p>Motifi492 – 504Nuclear localization signalSequence analysisAdd BLAST13

    Compositional bias

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    <p>This subsection of the ‘Family and Domains’ section describes the position of regions of compositional bias within the protein and the particular amino acids that are over-represented within those regions.<p><a href='/help/compbias' target='_top'>More...</a></p>Compositional biasi10 – 36Gly-richSequence analysisAdd BLAST27

    <p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

    Keywords - Domaini

    Coiled coil, Repeat

    Phylogenomic databases

    evolutionary genealogy of genes: Non-supervised Orthologous Groups

    More...
    eggNOGi
    KOG0616 Eukaryota
    ENOG410XPQQ LUCA

    Ensembl GeneTree

    More...
    GeneTreei
    ENSGT00940000166710

    The HOGENOM Database of Homologous Genes from Fully Sequenced Organisms

    More...
    HOGENOMi
    HOG000233033

    InParanoid: Eukaryotic Ortholog Groups

    More...
    InParanoidi
    O76360

    Database for complete collections of gene phylogenies

    More...
    PhylomeDBi
    O76360

    Family and domain databases

    Conserved Domains Database

    More...
    CDDi
    cd00038 CAP_ED, 2 hits
    cd05572 STKc_cGK, 1 hit

    Gene3D Structural and Functional Annotation of Protein Families

    More...
    Gene3Di
    2.60.120.10, 2 hits

    Integrated resource of protein families, domains and functional sites

    More...
    InterProi
    View protein in InterPro
    IPR000961 AGC-kinase_C
    IPR002374 cGMP_dep_kinase
    IPR018490 cNMP-bd-like
    IPR018488 cNMP-bd_CS
    IPR000595 cNMP-bd_dom
    IPR011009 Kinase-like_dom_sf
    IPR000719 Prot_kinase_dom
    IPR017441 Protein_kinase_ATP_BS
    IPR014710 RmlC-like_jellyroll
    IPR008271 Ser/Thr_kinase_AS
    IPR035014 STKc_cGK

    Pfam protein domain database

    More...
    Pfami
    View protein in Pfam
    PF00027 cNMP_binding, 2 hits
    PF00069 Pkinase, 1 hit

    PIRSF; a whole-protein classification database

    More...
    PIRSFi
    PIRSF000559 cGMP-dep_kinase, 1 hit

    Protein Motif fingerprint database; a protein domain database

    More...
    PRINTSi
    PR00104 CGMPKINASE

    Simple Modular Architecture Research Tool; a protein domain database

    More...
    SMARTi
    View protein in SMART
    SM00100 cNMP, 2 hits
    SM00133 S_TK_X, 1 hit
    SM00220 S_TKc, 1 hit

    Superfamily database of structural and functional annotation

    More...
    SUPFAMi
    SSF51206 SSF51206, 2 hits
    SSF56112 SSF56112, 1 hit

    PROSITE; a protein domain and family database

    More...
    PROSITEi
    View protein in PROSITE
    PS51285 AGC_KINASE_CTER, 1 hit
    PS00888 CNMP_BINDING_1, 2 hits
    PS00889 CNMP_BINDING_2, 2 hits
    PS50042 CNMP_BINDING_3, 2 hits
    PS00107 PROTEIN_KINASE_ATP, 1 hit
    PS50011 PROTEIN_KINASE_DOM, 1 hit
    PS00108 PROTEIN_KINASE_ST, 1 hit

    <p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence_length">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequences (6+)i

    <p>This subsection of the <a href="http://www.uniprot.org/help/sequences_section">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

    This entry describes 6 <p>This subsection of the ‘Sequence’ section lists the alternative protein sequences (isoforms) that can be generated from the same gene by a single or by the combination of up to four biological events (alternative promoter usage, alternative splicing, alternative initiation and ribosomal frameshifting). Additionally, this section gives relevant information on each alternative protein isoform.<p><a href='/help/alternative_products' target='_top'>More...</a></p> isoformsi produced by alternative splicing. AlignAdd to basket

    This entry has 6 described isoforms and 2 potential isoforms that are computationally mapped.Show allAlign All

    Isoform a2 Publications (identifier: O76360-1) [UniParc]FASTAAdd to basket

    This isoform has been chosen as the <div> <p><b>What is the canonical sequence?</b><p><a href='/help/canonical_and_isoforms' target='_top'>More...</a></p>canonicali sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide
            10         20         30         40         50
    MSSGSRPSSG GGGGGGGASG GAGGGAPGGG GGGIRGFFSK LRKPSDQPNG
    60 70 80 90 100
    NQVQVGTRTF EAHELQKLIP QLEEAISRKD AQLRQQQTIV EGHIKRISEL
    110 120 130 140 150
    EGEVTTLQRE CDKLRSVLEQ KAQSAASPGG QPPSPSPRTD QLGNDLQQKA
    160 170 180 190 200
    VLPADGVQRA KKIAVSAEPT NFENKPATLQ HYNKTVGAKQ MIRDAVQKND
    210 220 230 240 250
    FLKQLAKEQI IELVNCMYEM RARAGQWVIQ EGEPGDRLFV VAEGELQVSR
    260 270 280 290 300
    EGALLGKMRA GTVMGELAIL YNCTRTASVQ ALTDVQLWVL DRSVFQMITQ
    310 320 330 340 350
    RLGMERHSQL MNFLTKVSIF QNLSEDRISK MADVMDQDYY DGGHYIIRQG
    360 370 380 390 400
    EKGDAFFVIN SGQVKVTQQI EGETEPREIR VLNQGDFFGE RALLGEEVRT
    410 420 430 440 450
    ANIIAQAPGV EVLTLDRESF GKLIGDLESL KKDYGDKERL AQVVREPPSP
    460 470 480 490 500
    VKIVDDFREE FAQVTLKNVK RLATLGVGGF GRVELVCVNG DKAKTFALKA
    510 520 530 540 550
    LKKKHIVDTR QQEHIFAERN IMMETSTDWI VKLYKTFRDQ KFVYMLLEVC
    560 570 580 590 600
    LGGELWTTLR DRGHFDDYTA RFYVACVLEG LEYLHRKNIV YRDLKPENCL
    610 620 630 640 650
    LANTGYLKLV DFGFAKKLAS GRKTWTFCGT PEYVSPEIIL NKGHDQAADY
    660 670 680 690 700
    WALGIYICEL MLGRPPFQAS DPMKTYTLIL KGVDALEIPN RRIGKTATAL
    710 720 730 740 750
    VKKLCRDNPG ERLGSGSGGV NDIRKHRWFM GFDWEGLRSR TLKPPILPKV
    760 770 780
    SNPADVTNFD NYPPDNDVPP DEFSGWDEGF
    Length:780
    Mass (Da):86,742
    Last modified:November 1, 1999 - v2
    <p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:i55E036AF50626DD2
    GO
    Isoform b2 Publications (identifier: O76360-2) [UniParc]FASTAAdd to basket

    The sequence of this isoform differs from the canonical sequence as follows:
         1-43: Missing.
         44-52: PSDQPNGNQ → MKQQPPRIY

    Show »
    Length:737
    Mass (Da):83,354
    Checksum:iA3D63A797100B9C8
    GO
    Isoform c2 Publications (identifier: O76360-3) [UniParc]FASTAAdd to basket

    The sequence of this isoform differs from the canonical sequence as follows:
         1-20: MSSGSRPSSGGGGGGGGASG → MYGSSRHHMDSFSSNDGGAFL
         21-52: Missing.

    Show »
    Length:749
    Mass (Da):84,498
    Checksum:iADE0B9F6354D93C6
    GO
    Isoform d1 Publication (identifier: O76360-4) [UniParc]FASTAAdd to basket

    The sequence of this isoform differs from the canonical sequence as follows:
         1-188: Missing.
         189-275: KQMIRDAVQK...ELAILYNCTR → MLTCSTTTCQ...NLFYKRSHKK
         353-749: Missing.

    Note: No experimental confirmation available.Curated
    Show »
    Length:195
    Mass (Da):22,855
    Checksum:i37BEC2E725BBA168
    GO
    Isoform e1 Publication (identifier: O76360-5) [UniParc]FASTAAdd to basket

    The sequence of this isoform differs from the canonical sequence as follows:
         1-37: Missing.
         38-52: FSKLRKPSDQPNGNQ → MCWKFNPLKALRVVE

    Note: No experimental confirmation available.Curated
    Show »
    Length:743
    Mass (Da):84,028
    Checksum:i05386588EABC90AB
    GO
    Isoform f1 Publication (identifier: O76360-6) [UniParc]FASTAAdd to basket

    The sequence of this isoform differs from the canonical sequence as follows:
         1-310: Missing.
         311-316: MNFLTK → MKKVYV

    Note: No experimental confirmation available.Curated
    Show »
    Length:470
    Mass (Da):53,421
    Checksum:iB57D4D78EA028BCA
    GO

    <p>In eukaryotic reference proteomes, unreviewed entries that are likely to belong to the same gene are computationally mapped, based on gene identifiers from Ensembl, EnsemblGenomes and model organism databases.<p><a href='/help/gene_centric_isoform_mapping' target='_top'>More...</a></p>Computationally mapped potential isoform sequencesi

    There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
    EntryEntry nameProtein names
    Gene namesLengthAnnotation
    U4PEQ9U4PEQ9_CAEEL
    cGMP-dependent protein kinase
    egl-4 CELE_F55A8.2, F55A8.2
    783Annotation score:

    Annotation score:2 out of 5

    <p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the ‘correct annotation’ for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
    U4PRL6U4PRL6_CAEEL
    Uncharacterized protein
    egl-4 CELE_F55A8.2, F55A8.2
    484Annotation score:

    Annotation score:1 out of 5

    <p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the ‘correct annotation’ for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>

    Alternative sequence

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    <p>This subsection of the ‘Sequence’ section describes the sequence of naturally occurring alternative protein isoform(s). The changes in the amino acid sequence may be due to alternative splicing, alternative promoter usage, alternative initiation, or ribosomal frameshifting.<p><a href='/help/var_seq' target='_top'>More...</a></p>Alternative sequenceiVSP_0531701 – 310Missing in isoform f. 1 PublicationAdd BLAST310
    Alternative sequenceiVSP_0531711 – 188Missing in isoform d. 1 PublicationAdd BLAST188
    Alternative sequenceiVSP_0531721 – 43Missing in isoform b. 2 PublicationsAdd BLAST43
    Alternative sequenceiVSP_0531731 – 37Missing in isoform e. 1 PublicationAdd BLAST37
    Alternative sequenceiVSP_0531741 – 20MSSGS…GGASG → MYGSSRHHMDSFSSNDGGAF L in isoform c. 2 PublicationsAdd BLAST20
    Alternative sequenceiVSP_05317521 – 52Missing in isoform c. 2 PublicationsAdd BLAST32
    Alternative sequenceiVSP_05317638 – 52FSKLR…PNGNQ → MCWKFNPLKALRVVE in isoform e. 1 PublicationAdd BLAST15
    Alternative sequenceiVSP_05317744 – 52PSDQPNGNQ → MKQQPPRIY in isoform b. 2 Publications9
    Alternative sequenceiVSP_053178189 – 275KQMIR…YNCTR → MLTCSTTTCQISPAYPKFIE KLRRMIWGREPSTSYEFDEL AQQVALKSHRRNVDDGYYVE EIHFEPPQVVRKKQPTRNLF YKRSHKK in isoform d. 1 PublicationAdd BLAST87
    Alternative sequenceiVSP_053179311 – 316MNFLTK → MKKVYV in isoform f. 1 Publication6
    Alternative sequenceiVSP_053180353 – 749Missing in isoform d. 1 PublicationAdd BLAST397

    Sequence databases

    Select the link destinations:

    EMBL nucleotide sequence database

    More...
    EMBLi

    GenBank nucleotide sequence database

    More...
    GenBanki

    DNA Data Bank of Japan; a nucleotide sequence database

    More...
    DDBJi
    Links Updated
    FO081473 Genomic DNA Translation: CCD71859.1
    FO081473 Genomic DNA Translation: CCD71860.1
    FO081473 Genomic DNA Translation: CCD71861.1
    FO081473 Genomic DNA Translation: CCD71862.1
    FO081473 Genomic DNA Translation: CCD71863.1
    FO081473 Genomic DNA Translation: CCD71864.1

    NCBI Reference Sequences

    More...
    RefSeqi
    NP_001023223.1, NM_001028052.2 [O76360-5]
    NP_001023224.1, NM_001028053.2 [O76360-6]
    NP_500141.1, NM_067740.4 [O76360-1]
    NP_500142.1, NM_067741.3 [O76360-2]
    NP_741329.1, NM_171279.3 [O76360-3]
    NP_741330.2, NM_171280.2 [O76360-4]

    Genome annotation databases

    Ensembl metazoan genome annotation project

    More...
    EnsemblMetazoai
    F55A8.2a.1; F55A8.2a.1; WBGene00001173 [O76360-1]
    F55A8.2a.2; F55A8.2a.2; WBGene00001173 [O76360-1]
    F55A8.2b.1; F55A8.2b.1; WBGene00001173 [O76360-2]
    F55A8.2b.2; F55A8.2b.2; WBGene00001173 [O76360-2]
    F55A8.2c.1; F55A8.2c.1; WBGene00001173 [O76360-3]
    F55A8.2c.2; F55A8.2c.2; WBGene00001173 [O76360-3]
    F55A8.2c.3; F55A8.2c.3; WBGene00001173 [O76360-3]
    F55A8.2d.1; F55A8.2d.1; WBGene00001173 [O76360-4]
    F55A8.2e.1; F55A8.2e.1; WBGene00001173 [O76360-5]
    F55A8.2e.2; F55A8.2e.2; WBGene00001173 [O76360-5]
    F55A8.2f.1; F55A8.2f.1; WBGene00001173 [O76360-6]
    F55A8.2f.2; F55A8.2f.2; WBGene00001173 [O76360-6]

    Database of genes from NCBI RefSeq genomes

    More...
    GeneIDi
    176991

    UCSC genome browser

    More...
    UCSCi
    F55A8.2a.1 c. elegans

    Keywords - Coding sequence diversityi

    Alternative splicing

    <p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

    <p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    FO081473 Genomic DNA Translation: CCD71859.1
    FO081473 Genomic DNA Translation: CCD71860.1
    FO081473 Genomic DNA Translation: CCD71861.1
    FO081473 Genomic DNA Translation: CCD71862.1
    FO081473 Genomic DNA Translation: CCD71863.1
    FO081473 Genomic DNA Translation: CCD71864.1
    RefSeqiNP_001023223.1, NM_001028052.2 [O76360-5]
    NP_001023224.1, NM_001028053.2 [O76360-6]
    NP_500141.1, NM_067740.4 [O76360-1]
    NP_500142.1, NM_067741.3 [O76360-2]
    NP_741329.1, NM_171279.3 [O76360-3]
    NP_741330.2, NM_171280.2 [O76360-4]

    3D structure databases

    SMRiO76360
    ModBaseiSearch...

    Protein-protein interaction databases

    STRINGi6239.F55A8.2h.1

    Proteomic databases

    EPDiO76360
    PaxDbiO76360
    PeptideAtlasiO76360
    PRIDEiO76360

    Genome annotation databases

    EnsemblMetazoaiF55A8.2a.1; F55A8.2a.1; WBGene00001173 [O76360-1]
    F55A8.2a.2; F55A8.2a.2; WBGene00001173 [O76360-1]
    F55A8.2b.1; F55A8.2b.1; WBGene00001173 [O76360-2]
    F55A8.2b.2; F55A8.2b.2; WBGene00001173 [O76360-2]
    F55A8.2c.1; F55A8.2c.1; WBGene00001173 [O76360-3]
    F55A8.2c.2; F55A8.2c.2; WBGene00001173 [O76360-3]
    F55A8.2c.3; F55A8.2c.3; WBGene00001173 [O76360-3]
    F55A8.2d.1; F55A8.2d.1; WBGene00001173 [O76360-4]
    F55A8.2e.1; F55A8.2e.1; WBGene00001173 [O76360-5]
    F55A8.2e.2; F55A8.2e.2; WBGene00001173 [O76360-5]
    F55A8.2f.1; F55A8.2f.1; WBGene00001173 [O76360-6]
    F55A8.2f.2; F55A8.2f.2; WBGene00001173 [O76360-6]
    GeneIDi176991
    UCSCiF55A8.2a.1 c. elegans

    Organism-specific databases

    Comparative Toxicogenomics Database

    More...
    CTDi
    176991
    WormBaseiF55A8.2a ; CE19897 ; WBGene00001173 ; egl-4
    F55A8.2b ; CE19898 ; WBGene00001173 ; egl-4
    F55A8.2c ; CE31541 ; WBGene00001173 ; egl-4
    F55A8.2d ; CE37718 ; WBGene00001173 ; egl-4
    F55A8.2e ; CE37241 ; WBGene00001173 ; egl-4
    F55A8.2f ; CE37242 ; WBGene00001173 ; egl-4

    Phylogenomic databases

    eggNOGiKOG0616 Eukaryota
    ENOG410XPQQ LUCA
    GeneTreeiENSGT00940000166710
    HOGENOMiHOG000233033
    InParanoidiO76360
    PhylomeDBiO76360

    Enzyme and pathway databases

    ReactomeiR-CEL-1474151 Tetrahydrobiopterin (BH4) synthesis, recycling, salvage and regulation
    R-CEL-392517 Rap1 signalling
    SABIO-RKiO76360
    SignaLinkiO76360

    Miscellaneous databases

    Protein Ontology

    More...
    PROi
    PR:O76360

    Gene expression databases

    BgeeiWBGene00001173 Expressed in 5 organ(s), highest expression level in adult organism
    ExpressionAtlasiO76360 baseline and differential

    Family and domain databases

    CDDicd00038 CAP_ED, 2 hits
    cd05572 STKc_cGK, 1 hit
    Gene3Di2.60.120.10, 2 hits
    InterProiView protein in InterPro
    IPR000961 AGC-kinase_C
    IPR002374 cGMP_dep_kinase
    IPR018490 cNMP-bd-like
    IPR018488 cNMP-bd_CS
    IPR000595 cNMP-bd_dom
    IPR011009 Kinase-like_dom_sf
    IPR000719 Prot_kinase_dom
    IPR017441 Protein_kinase_ATP_BS
    IPR014710 RmlC-like_jellyroll
    IPR008271 Ser/Thr_kinase_AS
    IPR035014 STKc_cGK
    PfamiView protein in Pfam
    PF00027 cNMP_binding, 2 hits
    PF00069 Pkinase, 1 hit
    PIRSFiPIRSF000559 cGMP-dep_kinase, 1 hit
    PRINTSiPR00104 CGMPKINASE
    SMARTiView protein in SMART
    SM00100 cNMP, 2 hits
    SM00133 S_TK_X, 1 hit
    SM00220 S_TKc, 1 hit
    SUPFAMiSSF51206 SSF51206, 2 hits
    SSF56112 SSF56112, 1 hit
    PROSITEiView protein in PROSITE
    PS51285 AGC_KINASE_CTER, 1 hit
    PS00888 CNMP_BINDING_1, 2 hits
    PS00889 CNMP_BINDING_2, 2 hits
    PS50042 CNMP_BINDING_3, 2 hits
    PS00107 PROTEIN_KINASE_ATP, 1 hit
    PS50011 PROTEIN_KINASE_DOM, 1 hit
    PS00108 PROTEIN_KINASE_ST, 1 hit

    ProtoNet; Automatic hierarchical classification of proteins

    More...
    ProtoNeti
    Search...

    MobiDB: a database of protein disorder and mobility annotations

    More...
    MobiDBi
    Search...

    <p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

    <p>This subsection of the ‘Entry information’ section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiEGL4_CAEEL
    <p>This subsection of the ‘Entry information’ section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called ‘Primary (citable) accession number’.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: O76360
    Secondary accession number(s): Q688A8
    , Q688A9, Q7KPJ2, Q8MXG6, Q8MXG7
    <p>This subsection of the ‘Entry information’ section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification (‘Last modified’). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 15, 2009
    Last sequence update: November 1, 1999
    Last modified: October 16, 2019
    This is version 165 of the entry and version 2 of the sequence. See complete history.
    <p>This subsection of the ‘Entry information’ section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programCaenorhabditis annotation project

    <p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Caenorhabditis elegans
      Caenorhabditis elegans: entries, gene names and cross-references to WormBase
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