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Protein

Discs overgrown protein kinase

Gene

dco

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Involved in circadian rhythms, viability and molecular oscillations of the clock genes period (per) and timeless (tim). Dbt reduces the stability and thus the accumulation of monomeric per proteins, probably through phosphorylation. No evident circadian oscillation is detected in head. Together with CkIalpha, regulates processing of ci by phosphorylating it which promotes its binding to slmb, the F-box recognition component of the SCF(slmb) E3 ubiquitin-protein ligase (PubMed:16326393).4 Publications

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei38ATPPROSITE-ProRule annotation1
Active sitei128Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi15 – 23ATPPROSITE-ProRule annotation9

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionKinase, Serine/threonine-protein kinase, Transferase
Biological processBiological rhythms
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.7.11.1 1994
ReactomeiR-DME-201688 WNT mediated activation of DVL
R-DME-204005 COPII-mediated vesicle transport
R-DME-209155 Phosphorylation of AXN and APC
R-DME-209159 Assembly of the CI containing complexes
R-DME-209190 Phosphorylation of CI
R-DME-209214 Phosphorylation of SMO
R-DME-209360 Ubiquitination and proteolysis of phosphorylated CI
R-DME-209396 Phosphorylation of ARM
R-DME-209413 Assembly of the 'destruction complex'
R-DME-209440 Recruitment of the 'destruction complex' to the receptor complex, the degradation of AXN and release of ARM
R-DME-209461 Ubiquitination and degradation of phosphorylated ARM
R-DME-390023 Subcellular localisation of D
R-DME-432395 Degradation of TIM
R-DME-432490 Nuclear import of PER and TIM
R-DME-432501 Transcription repression by PER and activation by PDP1
R-DME-432524 Degradation of PER
R-DME-432553 Phosphorylation of PER and TIM
R-DME-432620 Dephosphorylation of PER
R-DME-451850 Positive regulation of FT and DS
R-DME-538898 Dephosphorylation of TIM
R-DME-6791226 Major pathway of rRNA processing in the nucleolus and cytosol
SignaLinkiO76324

Names & Taxonomyi

Protein namesi
Recommended name:
Discs overgrown protein kinase (EC:2.7.11.1)
Alternative name(s):
Protein double-time
Gene namesi
Name:dco
Synonyms:dbt
ORF Names:CG2048
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraHolometabolaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 3R

Organism-specific databases

FlyBaseiFBgn0002413 dco

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi47P → S in dbtS; shortens the behavioral period. 1 Publication1
Mutagenesisi80M → I in dbtL; lengthens the behavioral period. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001928491 – 440Discs overgrown protein kinaseAdd BLAST440

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei333Phosphoserine1 Publication1
Modified residuei334Phosphoserine1 Publication1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiO76324
PRIDEiO76324

PTM databases

iPTMnetiO76324

Expressioni

Tissue specificityi

Expressed in photoreceptor cells of the eyes as well as in the region situated between the optic lobe and the central brain.1 Publication

Gene expression databases

BgeeiFBgn0002413 Expressed in 58 organ(s), highest expression level in egg chamber
ExpressionAtlasiO76324 baseline and differential
GenevisibleiO76324 DM

Interactioni

Subunit structurei

Forms a complex with per (PubMed:9674431). Interacts with Dlish (PubMed:27692068).2 Publications

Protein-protein interaction databases

BioGridi68523, 35 interactors
DIPiDIP-46048N
IntActiO76324, 4 interactors
STRINGi7227.FBpp0085104

Structurei

3D structure databases

ProteinModelPortaliO76324
SMRiO76324
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini9 – 277Protein kinasePROSITE-ProRule annotationAdd BLAST269

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi319 – 332Poly-AlaAdd BLAST14
Compositional biasi336 – 339Poly-Gln4
Compositional biasi347 – 351Poly-Gly5
Compositional biasi414 – 426Poly-GlyAdd BLAST13
Compositional biasi430 – 437Poly-Gly8

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG1164 Eukaryota
ENOG410XPGP LUCA
GeneTreeiENSGT00930000150814
HOGENOMiHOG000148798
InParanoidiO76324
KOiK08960
OMAiRWYGTEC
OrthoDBiEOG091G0AFG
PhylomeDBiO76324

Family and domain databases

InterProiView protein in InterPro
IPR011009 Kinase-like_dom_sf
IPR000719 Prot_kinase_dom
IPR017441 Protein_kinase_ATP_BS
IPR008271 Ser/Thr_kinase_AS
PfamiView protein in Pfam
PF00069 Pkinase, 1 hit
SMARTiView protein in SMART
SM00220 S_TKc, 1 hit
SUPFAMiSSF56112 SSF56112, 1 hit
PROSITEiView protein in PROSITE
PS00107 PROTEIN_KINASE_ATP, 1 hit
PS50011 PROTEIN_KINASE_DOM, 1 hit
PS00108 PROTEIN_KINASE_ST, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All

O76324-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MELRVGNKYR LGRKIGSGSF GDIYLGTTIN TGEEVAIKLE CIRTKHPQLH
60 70 80 90 100
IESKFYKTMQ GGIGIPRIIW CGSEGDYNVM VMELLGPSLE DLFNFCSRRF
110 120 130 140 150
SLKTVLLLAD QMISRIDYIH SRDFIHRDIK PDNFLMGLGK KGNLVYIIDF
160 170 180 190 200
GLAKKFRDAR SLKHIPYREN KNLTGTARYA SINTHLGIEQ SRRDDLESLG
210 220 230 240 250
YVLMYFNLGA LPWQGLKAAN KRQKYERISE KKLSTSIVVL CKGFPSEFVN
260 270 280 290 300
YLNFCRQMHF DQRPDYCHLR KLFRNLFHRL GFTYDYVFDW NLLKFGGPRN
310 320 330 340 350
PQAIQQAQDG ADGQAGHDAV AAAAAVAAAA AASSHQQQQH KVNAALGGGG
360 370 380 390 400
GSAAQQQLQG GQTLAMLGGN GGGNGSQLIG GNGLNMDDSM AATNSSRPPY
410 420 430 440
DTPERRPSIR MRQGGGGGGG GVGVGGMPSG GGGGGVGNAK
Length:440
Mass (Da):47,958
Last modified:August 16, 2005 - v2
Checksum:i0B67B83E44213902
GO

Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
A0A0B4KHX3A0A0B4KHX3_DROME
Discs overgrown, isoform D
dco 01, 02, 0538, 09, 0915
440Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti353A → R in AAC39134 (PubMed:9674431).Curated1
Sequence conflicti428P → Q in AAC39134 (PubMed:9674431).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF055583 mRNA Translation: AAC39134.1
AF192484 Genomic DNA Translation: AAF27346.1
AE014297 Genomic DNA Translation: AAF57109.1
AE014297 Genomic DNA Translation: AAF57110.1
AF132558 mRNA Translation: AAD27857.1
RefSeqiNP_001263132.1, NM_001276203.1
NP_524602.1, NM_079863.3
NP_733414.1, NM_170535.2
NP_733415.1, NM_170536.3
UniGeneiDm.1901

Genome annotation databases

EnsemblMetazoaiFBtr0085742; FBpp0085104; FBgn0002413
FBtr0085743; FBpp0085105; FBgn0002413
FBtr0085744; FBpp0085106; FBgn0002413
FBtr0334548; FBpp0306615; FBgn0002413
GeneIDi43673
KEGGidme:Dmel_CG2048
UCSCiCG2048-RC d. melanogaster

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF055583 mRNA Translation: AAC39134.1
AF192484 Genomic DNA Translation: AAF27346.1
AE014297 Genomic DNA Translation: AAF57109.1
AE014297 Genomic DNA Translation: AAF57110.1
AF132558 mRNA Translation: AAD27857.1
RefSeqiNP_001263132.1, NM_001276203.1
NP_524602.1, NM_079863.3
NP_733414.1, NM_170535.2
NP_733415.1, NM_170536.3
UniGeneiDm.1901

3D structure databases

ProteinModelPortaliO76324
SMRiO76324
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi68523, 35 interactors
DIPiDIP-46048N
IntActiO76324, 4 interactors
STRINGi7227.FBpp0085104

PTM databases

iPTMnetiO76324

Proteomic databases

PaxDbiO76324
PRIDEiO76324

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiFBtr0085742; FBpp0085104; FBgn0002413
FBtr0085743; FBpp0085105; FBgn0002413
FBtr0085744; FBpp0085106; FBgn0002413
FBtr0334548; FBpp0306615; FBgn0002413
GeneIDi43673
KEGGidme:Dmel_CG2048
UCSCiCG2048-RC d. melanogaster

Organism-specific databases

CTDi43673
FlyBaseiFBgn0002413 dco

Phylogenomic databases

eggNOGiKOG1164 Eukaryota
ENOG410XPGP LUCA
GeneTreeiENSGT00930000150814
HOGENOMiHOG000148798
InParanoidiO76324
KOiK08960
OMAiRWYGTEC
OrthoDBiEOG091G0AFG
PhylomeDBiO76324

Enzyme and pathway databases

BRENDAi2.7.11.1 1994
ReactomeiR-DME-201688 WNT mediated activation of DVL
R-DME-204005 COPII-mediated vesicle transport
R-DME-209155 Phosphorylation of AXN and APC
R-DME-209159 Assembly of the CI containing complexes
R-DME-209190 Phosphorylation of CI
R-DME-209214 Phosphorylation of SMO
R-DME-209360 Ubiquitination and proteolysis of phosphorylated CI
R-DME-209396 Phosphorylation of ARM
R-DME-209413 Assembly of the 'destruction complex'
R-DME-209440 Recruitment of the 'destruction complex' to the receptor complex, the degradation of AXN and release of ARM
R-DME-209461 Ubiquitination and degradation of phosphorylated ARM
R-DME-390023 Subcellular localisation of D
R-DME-432395 Degradation of TIM
R-DME-432490 Nuclear import of PER and TIM
R-DME-432501 Transcription repression by PER and activation by PDP1
R-DME-432524 Degradation of PER
R-DME-432553 Phosphorylation of PER and TIM
R-DME-432620 Dephosphorylation of PER
R-DME-451850 Positive regulation of FT and DS
R-DME-538898 Dephosphorylation of TIM
R-DME-6791226 Major pathway of rRNA processing in the nucleolus and cytosol
SignaLinkiO76324

Miscellaneous databases

ChiTaRSidco fly
GenomeRNAii43673
PROiPR:O76324

Gene expression databases

BgeeiFBgn0002413 Expressed in 58 organ(s), highest expression level in egg chamber
ExpressionAtlasiO76324 baseline and differential
GenevisibleiO76324 DM

Family and domain databases

InterProiView protein in InterPro
IPR011009 Kinase-like_dom_sf
IPR000719 Prot_kinase_dom
IPR017441 Protein_kinase_ATP_BS
IPR008271 Ser/Thr_kinase_AS
PfamiView protein in Pfam
PF00069 Pkinase, 1 hit
SMARTiView protein in SMART
SM00220 S_TKc, 1 hit
SUPFAMiSSF56112 SSF56112, 1 hit
PROSITEiView protein in PROSITE
PS00107 PROTEIN_KINASE_ATP, 1 hit
PS50011 PROTEIN_KINASE_DOM, 1 hit
PS00108 PROTEIN_KINASE_ST, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiDCO_DROME
AccessioniPrimary (citable) accession number: O76324
Secondary accession number(s): A4V3P5, Q0KHY4, Q9V462
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 15, 1999
Last sequence update: August 16, 2005
Last modified: November 7, 2018
This is version 158 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
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Main funding by: National Institutes of Health

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