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Protein

Alpha-amylase-related protein

Gene

Amyrel

Organism
Drosophila yakuba (Fruit fly)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalytic activityi

Endohydrolysis of (1->4)-alpha-D-glucosidic linkages in polysaccharides containing three or more (1->4)-alpha-linked D-glucose units.By similarity

Cofactori

Protein has several cofactor binding sites:

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi117CalciumBy similarity1
Metal bindingi168Calcium; via carbonyl oxygenBy similarity1
Metal bindingi177CalciumBy similarity1
Binding sitei205ChlorideBy similarity1
Active sitei207NucleophileBy similarity1
Metal bindingi211Calcium; via carbonyl oxygenBy similarity1
Active sitei244Proton donorBy similarity1
Binding sitei307ChlorideBy similarity1
Sitei309Transition state stabilizerBy similarity1
Binding sitei342ChlorideBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionGlycosidase, Hydrolase
Biological processCarbohydrate metabolism
LigandCalcium, Chloride, Metal-binding

Protein family/group databases

CAZyiGH13 Glycoside Hydrolase Family 13

Names & Taxonomyi

Protein namesi
Recommended name:
Alpha-amylase-related protein (EC:3.2.1.1By similarity)
Gene namesi
Name:Amyrel
OrganismiDrosophila yakuba (Fruit fly)
Taxonomic identifieri7245 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraHolometabolaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora

Organism-specific databases

FlyBaseiFBgn0025015 Dyak\Amyrel

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 19By similarityAdd BLAST19
ChainiPRO_000000139420 – 493Alpha-amylase-related proteinAdd BLAST474

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei20Pyrrolidone carboxylic acidBy similarity1
Disulfide bondi47 ↔ 103By similarity
Disulfide bondi156 ↔ 170By similarity
Disulfide bondi375 ↔ 381By similarity
Disulfide bondi417 ↔ 440Sequence analysis
Disulfide bondi447 ↔ 459By similarity

Keywords - PTMi

Disulfide bond, Pyrrolidone carboxylic acid

Interactioni

Subunit structurei

Monomer.By similarity

Structurei

3D structure databases

ProteinModelPortaliO76264
SMRiO76264
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 13 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG2212 Eukaryota
COG0366 LUCA
OrthoDBiEOG091G07V6

Family and domain databases

Gene3Di2.60.40.1180, 1 hit
InterProiView protein in InterPro
IPR006048 A-amylase/branching_C
IPR031319 A-amylase_C
IPR006046 Alpha_amylase
IPR006047 Glyco_hydro_13_cat_dom
IPR013780 Glyco_hydro_b
IPR017853 Glycoside_hydrolase_SF
PfamiView protein in Pfam
PF00128 Alpha-amylase, 1 hit
PF02806 Alpha-amylase_C, 1 hit
PRINTSiPR00110 ALPHAAMYLASE
SMARTiView protein in SMART
SM00642 Aamy, 1 hit
SM00632 Aamy_C, 1 hit
SUPFAMiSSF51445 SSF51445, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O76264-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MFKLALTLTL CLAGSLSLAQ HNPHWWGNRN TIVHLFEWKW SDIAQECENF
60 70 80 90 100
LGPRGFAGVQ VSPVNENIIS AGRPWWERYQ PISYKLTTRS GNEEEFGDMV
110 120 130 140 150
RRCNDVGVRI YVDVLLNHMS GDFDGVAVGT AGTEAEPGKK SFPGVPYSAQ
160 170 180 190 200
DFHPTCEITD WNDRFQVQQC ELVGLKDLDQ SSDWVRSKPI EFLDHLIELG
210 220 230 240 250
VAGFRVDAAK HMASEDLEYI YSSLSNLNID HGFPHNARPF IFQEVIDHGH
260 270 280 290 300
ETVSRDEYKD LGAVTEFRFS EEIGNAFRGN NALKWLQSWG TGWGFLPSGQ
310 320 330 340 350
ALTFVDNHDN QRDAGAVLNY KSPKQYKMAT AFHLAYPYGI SRVMSSFAFD
360 370 380 390 400
DHDTPPPQDA QERIISPEFD EDGACVNGWI CEHRWRQIYA MVGFKNAVRD
410 420 430 440 450
TEITGWWDNG DNQISFCRGN KGFLAINNNL YDLSQDLNTC LPQGTYCDVI
460 470 480 490
SGSLIDGSCT GKSVTVNEHG YGYIHIGSDD FDGVLALHVD AKV
Length:493
Mass (Da):55,420
Last modified:May 1, 2000 - v2
Checksum:iD95D63C49C3E1028
GO

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti57A → G in strain: LBV2. 1
Natural varianti70S → A in strain: LBV2, SA3 and SA4. 1
Natural varianti148S → T in strain: LO4, LBV2, SA3 and SA4. 1
Natural varianti189P → L in strain: LO4, LBV2, SA3 and SA4. 1
Natural varianti198E → D in strain: LBV2, SA3 and SA4. 1
Natural varianti360A → E in strain: LO4, LBV2, SA3 and SA4. 1
Natural varianti365I → V in strain: LBV2, SA3 and SA4. 1
Natural varianti401T → A in strain: SA3. 1
Natural varianti401T → M in strain: LO4. 1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF039561 Genomic DNA Translation: AAC39096.2
AF280874 Genomic DNA Translation: AAG60601.1
AF280875 Genomic DNA Translation: AAG60602.1
AF280876 Genomic DNA Translation: AAG60603.1
AF280877 Genomic DNA Translation: AAG60604.1
AF280878 Genomic DNA Translation: AAG60605.1

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF039561 Genomic DNA Translation: AAC39096.2
AF280874 Genomic DNA Translation: AAG60601.1
AF280875 Genomic DNA Translation: AAG60602.1
AF280876 Genomic DNA Translation: AAG60603.1
AF280877 Genomic DNA Translation: AAG60604.1
AF280878 Genomic DNA Translation: AAG60605.1

3D structure databases

ProteinModelPortaliO76264
SMRiO76264
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

CAZyiGH13 Glycoside Hydrolase Family 13

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Organism-specific databases

FlyBaseiFBgn0025015 Dyak\Amyrel

Phylogenomic databases

eggNOGiKOG2212 Eukaryota
COG0366 LUCA
OrthoDBiEOG091G07V6

Family and domain databases

Gene3Di2.60.40.1180, 1 hit
InterProiView protein in InterPro
IPR006048 A-amylase/branching_C
IPR031319 A-amylase_C
IPR006046 Alpha_amylase
IPR006047 Glyco_hydro_13_cat_dom
IPR013780 Glyco_hydro_b
IPR017853 Glycoside_hydrolase_SF
PfamiView protein in Pfam
PF00128 Alpha-amylase, 1 hit
PF02806 Alpha-amylase_C, 1 hit
PRINTSiPR00110 ALPHAAMYLASE
SMARTiView protein in SMART
SM00642 Aamy, 1 hit
SM00632 Aamy_C, 1 hit
SUPFAMiSSF51445 SSF51445, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiAMYR_DROYA
AccessioniPrimary (citable) accession number: O76264
Secondary accession number(s): Q9BH40
, Q9BN03, Q9BN04, Q9BN05
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 26, 2001
Last sequence update: May 1, 2000
Last modified: February 28, 2018
This is version 107 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  3. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
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Main funding by: National Institutes of Health

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