UniProtKB - O76081 (RGS20_HUMAN)
Protein
Regulator of G-protein signaling 20
Gene
RGS20
Organism
Homo sapiens (Human)
Status
Functioni
Inhibits signal transduction by increasing the GTPase activity of G protein alpha subunits thereby driving them into their inactive GDP-bound form. Binds selectively to G(z)-alpha and G(alpha)-i2 subunits, accelerates their GTPase activity and regulates their signaling activities. The G(z)-alpha activity is inhibited by the phosphorylation and palmitoylation of the G-protein. Negatively regulates mu-opioid receptor-mediated activation of the G-proteins (By similarity).By similarity1 Publication
GO - Molecular functioni
- GTPase activator activity Source: ProtInc
- GTPase activity Source: Reactome
GO - Biological processi
- G protein-coupled receptor signaling pathway Source: Reactome
- negative regulation of signal transduction Source: UniProtKB-KW
- regulation of G protein-coupled receptor signaling pathway Source: ProtInc
Keywordsi
Molecular function | Signal transduction inhibitor |
Enzyme and pathway databases
PathwayCommonsi | O76081 |
Reactomei | R-HSA-418594, G alpha (i) signalling events R-HSA-418597, G alpha (z) signalling events |
Names & Taxonomyi
Protein namesi | Recommended name: Regulator of G-protein signaling 20Short name: RGS20 Alternative name(s): Gz-selective GTPase-activating protein Short name: G(z)GAP Short name: Gz-GAP Regulator of G-protein signaling Z1 Regulator of Gz-selective protein signaling 1 |
Gene namesi | Name:RGS20 Synonyms:RGSZ1, ZGAP1 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:14600, RGS20 |
MIMi | 607193, gene |
neXtProti | NX_O76081 |
VEuPathDBi | HostDB:ENSG00000147509.13 |
Subcellular locationi
Nucleus
Other locations
Note: Shuttles between the cytoplasm/cell membrane and the nucleus. Anchored to the membrane through palmitoylation.By similarity
Golgi apparatus
- trans-Golgi network Source: CACAO
Nucleus
- nucleus Source: UniProtKB-SubCell
Plasma Membrane
- plasma membrane Source: Reactome
Other locations
- cytoplasm Source: CACAO
Keywords - Cellular componenti
Cytoplasm, Membrane, NucleusPathology & Biotechi
Organism-specific databases
DisGeNETi | 8601 |
OpenTargetsi | ENSG00000147509 |
PharmGKBi | PA34373 |
Miscellaneous databases
Pharosi | O76081, Tbio |
Genetic variation databases
BioMutai | RGS20 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000204233 | 1 – 388 | Regulator of G-protein signaling 20Add BLAST | 388 |
Post-translational modificationi
Fatty acylated. Heavily palmitoylated in the cysteine string motif (By similarity).By similarity
N- and O-glycosylated in synapsomal membranes.By similarity
Serine phosphorylated in synapsomal membranes.By similarity
Sumoylated with SUMO1 and SUMO2 in synaptosomes. The sumoylated forms act as a scaffold for sequestering mu-opioid receptor-activated G(alpha) subunits (By similarity).By similarity
Keywords - PTMi
Glycoprotein, Lipoprotein, Palmitate, Phosphoprotein, Ubl conjugationProteomic databases
jPOSTi | O76081 |
MassIVEi | O76081 |
MaxQBi | O76081 |
PaxDbi | O76081 |
PeptideAtlasi | O76081 |
PRIDEi | O76081 |
ProteomicsDBi | 50382 [O76081-1] 50383 [O76081-2] 50384 [O76081-3] 50385 [O76081-4] 50386 [O76081-5] 50387 [O76081-6] |
PTM databases
iPTMneti | O76081 |
PhosphoSitePlusi | O76081 |
SwissPalmi | O76081 |
Expressioni
Tissue specificityi
Isoform 5 is expressed in brain at high levels in the caudate nucleus and temporal lobe.
Gene expression databases
Bgeei | ENSG00000147509, Expressed in caudate nucleus and 138 other tissues |
ExpressionAtlasi | O76081, baseline and differential |
Genevisiblei | O76081, HS |
Organism-specific databases
HPAi | ENSG00000147509, Tissue enriched (brain) |
Interactioni
Subunit structurei
Forms a complex with G(alpha)z/i2 subunits and mu-opioid receptors; the formation of this complex results in mu-opioid receptor desensitization.
Interacts with OPRM1 (By similarity).
By similarityBinary interactionsi
O76081
Isoform 5 [O76081-6]
Protein-protein interaction databases
BioGRIDi | 114161, 107 interactors |
CORUMi | O76081 |
IntActi | O76081, 53 interactors |
MINTi | O76081 |
STRINGi | 9606.ENSP00000297313 |
Miscellaneous databases
RNActi | O76081, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 262 – 378 | RGSPROSITE-ProRule annotationAdd BLAST | 117 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 208 – 220 | Poly-CysAdd BLAST | 13 |
Phylogenomic databases
eggNOGi | KOG3589, Eukaryota |
GeneTreei | ENSGT00940000159123 |
HOGENOMi | CLU_059863_0_0_1 |
InParanoidi | O76081 |
OMAi | HQTMENE |
OrthoDBi | 1409647at2759 |
PhylomeDBi | O76081 |
TreeFami | TF315837 |
Family and domain databases
InterProi | View protein in InterPro IPR016137, RGS IPR036305, RGS_sf |
Pfami | View protein in Pfam PF00615, RGS, 1 hit |
PRINTSi | PR01301, RGSPROTEIN |
SMARTi | View protein in SMART SM00315, RGS, 1 hit |
SUPFAMi | SSF48097, SSF48097, 1 hit |
PROSITEi | View protein in PROSITE PS50132, RGS, 1 hit |
s (6+)i Sequence
Sequence statusi: Complete.
This entry describes 6 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 6 described isoforms and 3 potential isoforms that are computationally mapped.Show allAlign All
Isoform 6 (identifier: O76081-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MPQLSQDNQE CLQKHFSRPS IWTQFLPLFR AQRYNTDIHQ ITENEGDLRA
60 70 80 90 100
VPDIKSFPPA QLPDSPAAPK LFGLLSSPLS SLARFFSHLL RRPPPEAPRR
110 120 130 140 150
RLDFSPLLPA LPAARLSRGH EELPGRLSLL LGAALALPGR PSGGRPLRPP
160 170 180 190 200
HPVAKPREED ATAGQSSPMP QMGSERMEMR KRQMPAAQDT PGAAPGQPGA
210 220 230 240 250
GSRGSNACCF CWCCCCSCSC LTVRNQEDQR PTIASHELRA DLPTWEESPA
260 270 280 290 300
PTLEEVNAWA QSFDKLMVTP AGRNAFREFL RTEFSEENML FWMACEELKK
310 320 330 340 350
EANKNIIEEK ARIIYEDYIS ILSPKEVSLD SRVREVINRN MVEPSQHIFD
360 370 380
DAQLQIYTLM HRDSYPRFMN SAVYKDLLQS LSEKSIEA
Computationally mapped potential isoform sequencesi
There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketB3KSW4 | B3KSW4_HUMAN | Regulator of G-protein-signaling 20 | RGS20 | 122 | Annotation score: | ||
E5RH54 | E5RH54_HUMAN | Regulator of G-protein-signaling 20 | RGS20 | 70 | Annotation score: | ||
E5RGA3 | E5RGA3_HUMAN | Regulator of G-protein-signaling 20 | RGS20 | 58 | Annotation score: |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_005698 | 1 – 236 | Missing in isoform 3. 1 PublicationAdd BLAST | 236 | |
Alternative sequenceiVSP_005697 | 1 – 208 | Missing in isoform 2. 1 PublicationAdd BLAST | 208 | |
Alternative sequenceiVSP_005694 | 1 – 55 | MPQLS…VPDIK → MRTADGGEPAGASSPAGRVD GGL in isoform 5. 2 PublicationsAdd BLAST | 55 | |
Alternative sequenceiVSP_005695 | 3 – 55 | Missing in isoform 4. 2 PublicationsAdd BLAST | 53 | |
Alternative sequenceiVSP_005696 | 56 – 170 | Missing in isoform 1, isoform 4 and isoform 5. 5 PublicationsAdd BLAST | 115 | |
Alternative sequenceiVSP_005699 | 209 – 220 | CFCWC…CSCSC → MKETSGLFLISS in isoform 2. 1 PublicationAdd BLAST | 12 | |
Alternative sequenceiVSP_005700 | 237 – 247 | ELRADLPTWEE → MKETSGLFLIS in isoform 3. 1 PublicationAdd BLAST | 11 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF060877 mRNA Translation: AAC62009.2 AF366054 mRNA Translation: AAK54122.1 AF366055 mRNA Translation: AAK54123.1 AF366056 mRNA Translation: AAK54124.1 AF366057 mRNA Translation: AAK54125.1 AF074979 mRNA Translation: AAC62013.1 AF493940 mRNA Translation: AAM12654.1 BC015614 mRNA Translation: AAH15614.2 BC063490 mRNA Translation: AAH63490.1 AY046538 mRNA Translation: AAL03971.1 |
CCDSi | CCDS6155.1 [O76081-1] CCDS6156.1 [O76081-6] CCDS69482.1 [O76081-2] |
RefSeqi | NP_001273602.1, NM_001286673.1 [O76081-2] NP_001273603.1, NM_001286674.1 [O76081-4] NP_001273604.1, NM_001286675.1 [O76081-3] NP_003693.2, NM_003702.4 [O76081-6] NP_733466.1, NM_170587.3 [O76081-1] XP_011515924.1, XM_011517622.2 |
Genome annotation databases
Ensembli | ENST00000276500; ENSP00000276500; ENSG00000147509 [O76081-6] ENST00000297313; ENSP00000297313; ENSG00000147509 [O76081-1] ENST00000344277; ENSP00000344630; ENSG00000147509 [O76081-2] |
GeneIDi | 8601 |
KEGGi | hsa:8601 |
UCSCi | uc003xrp.5, human [O76081-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF060877 mRNA Translation: AAC62009.2 AF366054 mRNA Translation: AAK54122.1 AF366055 mRNA Translation: AAK54123.1 AF366056 mRNA Translation: AAK54124.1 AF366057 mRNA Translation: AAK54125.1 AF074979 mRNA Translation: AAC62013.1 AF493940 mRNA Translation: AAM12654.1 BC015614 mRNA Translation: AAH15614.2 BC063490 mRNA Translation: AAH63490.1 AY046538 mRNA Translation: AAL03971.1 |
CCDSi | CCDS6155.1 [O76081-1] CCDS6156.1 [O76081-6] CCDS69482.1 [O76081-2] |
RefSeqi | NP_001273602.1, NM_001286673.1 [O76081-2] NP_001273603.1, NM_001286674.1 [O76081-4] NP_001273604.1, NM_001286675.1 [O76081-3] NP_003693.2, NM_003702.4 [O76081-6] NP_733466.1, NM_170587.3 [O76081-1] XP_011515924.1, XM_011517622.2 |
3D structure databases
BMRBi | O76081 |
SMRi | O76081 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 114161, 107 interactors |
CORUMi | O76081 |
IntActi | O76081, 53 interactors |
MINTi | O76081 |
STRINGi | 9606.ENSP00000297313 |
PTM databases
iPTMneti | O76081 |
PhosphoSitePlusi | O76081 |
SwissPalmi | O76081 |
Genetic variation databases
BioMutai | RGS20 |
Proteomic databases
jPOSTi | O76081 |
MassIVEi | O76081 |
MaxQBi | O76081 |
PaxDbi | O76081 |
PeptideAtlasi | O76081 |
PRIDEi | O76081 |
ProteomicsDBi | 50382 [O76081-1] 50383 [O76081-2] 50384 [O76081-3] 50385 [O76081-4] 50386 [O76081-5] 50387 [O76081-6] |
Protocols and materials databases
Antibodypediai | 11664, 161 antibodies |
DNASUi | 8601 |
Genome annotation databases
Ensembli | ENST00000276500; ENSP00000276500; ENSG00000147509 [O76081-6] ENST00000297313; ENSP00000297313; ENSG00000147509 [O76081-1] ENST00000344277; ENSP00000344630; ENSG00000147509 [O76081-2] |
GeneIDi | 8601 |
KEGGi | hsa:8601 |
UCSCi | uc003xrp.5, human [O76081-1] |
Organism-specific databases
CTDi | 8601 |
DisGeNETi | 8601 |
GeneCardsi | RGS20 |
HGNCi | HGNC:14600, RGS20 |
HPAi | ENSG00000147509, Tissue enriched (brain) |
MIMi | 607193, gene |
neXtProti | NX_O76081 |
OpenTargetsi | ENSG00000147509 |
PharmGKBi | PA34373 |
VEuPathDBi | HostDB:ENSG00000147509.13 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG3589, Eukaryota |
GeneTreei | ENSGT00940000159123 |
HOGENOMi | CLU_059863_0_0_1 |
InParanoidi | O76081 |
OMAi | HQTMENE |
OrthoDBi | 1409647at2759 |
PhylomeDBi | O76081 |
TreeFami | TF315837 |
Enzyme and pathway databases
PathwayCommonsi | O76081 |
Reactomei | R-HSA-418594, G alpha (i) signalling events R-HSA-418597, G alpha (z) signalling events |
Miscellaneous databases
BioGRID-ORCSi | 8601, 3 hits in 879 CRISPR screens |
ChiTaRSi | RGS20, human |
GeneWikii | RGS20 |
GenomeRNAii | 8601 |
Pharosi | O76081, Tbio |
PROi | PR:O76081 |
RNActi | O76081, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000147509, Expressed in caudate nucleus and 138 other tissues |
ExpressionAtlasi | O76081, baseline and differential |
Genevisiblei | O76081, HS |
Family and domain databases
InterProi | View protein in InterPro IPR016137, RGS IPR036305, RGS_sf |
Pfami | View protein in Pfam PF00615, RGS, 1 hit |
PRINTSi | PR01301, RGSPROTEIN |
SMARTi | View protein in SMART SM00315, RGS, 1 hit |
SUPFAMi | SSF48097, SSF48097, 1 hit |
PROSITEi | View protein in PROSITE PS50132, RGS, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | RGS20_HUMAN | |
Accessioni | O76081Primary (citable) accession number: O76081 Secondary accession number(s): Q96BG9 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | June 20, 2001 |
Last sequence update: | October 18, 2001 | |
Last modified: | February 10, 2021 | |
This is version 191 of the entry and version 4 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Human chromosome 8
Human chromosome 8: entries, gene names and cross-references to MIM - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot