UniProtKB - O76080 (ZFAN5_HUMAN)
Protein
AN1-type zinc finger protein 5
Gene
ZFAND5
Organism
Homo sapiens (Human)
Status
Functioni
Involved in protein degradation via the ubiquitin-proteasome system. May act by anchoring ubiquitinated proteins to the proteasome. Plays a role in ubiquitin-mediated protein degradation during muscle atrophy. Plays a role in the regulation of NF-kappa-B activation and apoptosis. Inhibits NF-kappa-B activation triggered by overexpression of RIPK1 and TRAF6 but not of RELA. Inhibits also tumor necrosis factor (TNF), IL-1 and TLR4-induced NF-kappa-B activation in a dose-dependent manner. Overexpression sensitizes cells to TNF-induced apoptosis. Is a potent inhibitory factor for osteoclast differentiation.1 Publication
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 8 – 42 | A20-typePROSITE-ProRule annotationAdd BLAST | 35 | |
Zinc fingeri | 151 – 194 | AN1-typePROSITE-ProRule annotationAdd BLAST | 44 |
GO - Molecular functioni
- DNA binding Source: InterPro
- zinc ion binding Source: InterPro
GO - Biological processi
- face development Source: Ensembl
- fibroblast migration Source: Ensembl
- in utero embryonic development Source: Ensembl
- platelet-derived growth factor receptor signaling pathway Source: Ensembl
- respiratory system process Source: Ensembl
- skeletal system morphogenesis Source: Ensembl
- smooth muscle tissue development Source: Ensembl
- vasculature development Source: Ensembl
Keywordsi
Biological process | Ubl conjugation pathway |
Ligand | Metal-binding, Zinc |
Enzyme and pathway databases
PathwayCommonsi | O76080 |
Names & Taxonomyi
Protein namesi | Recommended name: AN1-type zinc finger protein 5Alternative name(s): Zinc finger A20 domain-containing protein 2 Zinc finger protein 216 |
Gene namesi | Name:ZFAND5 Synonyms:ZA20D2, ZNF216 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:13008, ZFAND5 |
MIMi | 604761, gene |
neXtProti | NX_O76080 |
VEuPathDBi | HostDB:ENSG00000107372.12 |
Subcellular locationi
Cytoplasm and Cytosol
- Cytoplasm By similarity
Other locations
- cytoplasm Source: UniProtKB-SubCell
Keywords - Cellular componenti
CytoplasmPathology & Biotechi
Organism-specific databases
DisGeNETi | 7763 |
OpenTargetsi | ENSG00000107372 |
PharmGKBi | PA37587 |
Miscellaneous databases
Pharosi | O76080, Tbio |
Genetic variation databases
BioMutai | ZFAND5 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000066557 | 1 – 213 | AN1-type zinc finger protein 5Add BLAST | 213 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 48 | PhosphoserineCombined sources | 1 | |
Modified residuei | 58 | PhosphoserineBy similarity | 1 | |
Modified residuei | 209 | N6-acetyllysineBy similarity | 1 |
Keywords - PTMi
Acetylation, PhosphoproteinProteomic databases
EPDi | O76080 |
jPOSTi | O76080 |
MassIVEi | O76080 |
MaxQBi | O76080 |
PaxDbi | O76080 |
PeptideAtlasi | O76080 |
PRIDEi | O76080 |
ProteomicsDBi | 50381 |
TopDownProteomicsi | O76080 |
PTM databases
iPTMneti | O76080 |
PhosphoSitePlusi | O76080 |
Expressioni
Tissue specificityi
Highly expressed in skeletal muscle. Expressed in fetal cochlea. Also expressed in infant brain, fetal heart, pancreatic islet, melanocyte, pineal gland, placenta, corneal stroma, and parathyroid tumor. Weakly expressed or undetectable in adult brain, heart, colon, thymus, spleen, kidney, liver, small intestine, placenta, lung and peripheral blood leukocytes. Expressed in rhabdomyosarcoma RD cells (at protein level).2 Publications
Gene expression databases
Bgeei | ENSG00000107372, Expressed in tibia and 241 other tissues |
ExpressionAtlasi | O76080, baseline and differential |
Genevisiblei | O76080, HS |
Organism-specific databases
HPAi | ENSG00000107372, Tissue enhanced (skeletal) |
Interactioni
Subunit structurei
Interacts with ubiquitin and polyubiquitinated proteins.
Identified in a heterotrimeric complex with ubiquitin and SQSTM1, where ZFAND5 and SQSTM1 both interact with the same ubiquitin molecule (By similarity). Homooligomer and/or heterooligomer.
Interacts (via A20-type domain) with IKBKG and RIPK1 and with TRAF6 (via AN1-type domain).
By similarity1 PublicationBinary interactionsi
O76080
With | #Exp. | IntAct |
---|---|---|
UBC [P0CG48] | 3 | EBI-8028844,EBI-3390054 |
Protein-protein interaction databases
BioGRIDi | 113546, 22 interactors |
IntActi | O76080, 10 interactors |
MINTi | O76080 |
STRINGi | 9606.ENSP00000237937 |
Miscellaneous databases
RNActi | O76080, protein |
Family & Domainsi
Domaini
The A20-type zinc finger directly binds polyubiquitin chains and associates with the 26S proteasome. The zinc-finger A20-type domain is essential for inhibition of NF-kappa-B activation (By similarity).By similarity
Zinc finger
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 8 – 42 | A20-typePROSITE-ProRule annotationAdd BLAST | 35 | |
Zinc fingeri | 151 – 194 | AN1-typePROSITE-ProRule annotationAdd BLAST | 44 |
Keywords - Domaini
Zinc-fingerPhylogenomic databases
eggNOGi | KOG3173, Eukaryota |
GeneTreei | ENSGT00940000156165 |
HOGENOMi | CLU_057016_1_0_1 |
InParanoidi | O76080 |
OMAi | EMSISCE |
OrthoDBi | 1551371at2759 |
PhylomeDBi | O76080 |
TreeFami | TF313612 |
Family and domain databases
Gene3Di | 4.10.1110.10, 1 hit |
InterProi | View protein in InterPro IPR035896, AN1-like_Znf IPR002653, Znf_A20 IPR000058, Znf_AN1 |
Pfami | View protein in Pfam PF01754, zf-A20, 1 hit PF01428, zf-AN1, 1 hit |
SMARTi | View protein in SMART SM00259, ZnF_A20, 1 hit SM00154, ZnF_AN1, 1 hit |
SUPFAMi | SSF118310, SSF118310, 1 hit |
PROSITEi | View protein in PROSITE PS51036, ZF_A20, 1 hit PS51039, ZF_AN1, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
O76080-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MAQETNQTPG PMLCSTGCGF YGNPRTNGMC SVCYKEHLQR QQNSGRMSPM
60 70 80 90 100
GTASGSNSPT SDSASVQRAD TSLNNCEGAA GSTSEKSRNV PVAALPVTQQ
110 120 130 140 150
MTEMSISRED KITTPKTEVS EPVVTQPSPS VSQPSTSQSE EKAPELPKPK
160 170 180 190 200
KNRCFMCRKK VGLTGFDCRC GNLFCGLHRY SDKHNCPYDY KAEAAAKIRK
210
ENPVVVAEKI QRI
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketQ5QPB1 | Q5QPB1_HUMAN | AN1-type zinc finger protein 5 | ZFAND5 | 211 | Annotation score: |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF062072 Genomic DNA Translation: AAC61801.1 AF062346 mRNA Translation: AAC42601.1 AF062347 mRNA Translation: AAC42602.1 AK290849 mRNA Translation: BAF83538.1 AL135924 Genomic DNA No translation available. CH471089 Genomic DNA Translation: EAW62533.1 BC011018 mRNA Translation: AAH11018.1 BC027707 mRNA Translation: AAH27707.1 BC073131 mRNA Translation: AAH73131.1 |
CCDSi | CCDS6642.1 |
RefSeqi | NP_001095890.1, NM_001102420.2 NP_001095891.1, NM_001102421.2 NP_001265172.1, NM_001278243.1 NP_001265173.1, NM_001278244.1 NP_001265174.1, NM_001278245.1 NP_005998.1, NM_006007.3 |
Genome annotation databases
Ensembli | ENST00000237937; ENSP00000237937; ENSG00000107372 ENST00000343431; ENSP00000350586; ENSG00000107372 ENST00000376960; ENSP00000366159; ENSG00000107372 ENST00000376962; ENSP00000366161; ENSG00000107372 |
GeneIDi | 7763 |
KEGGi | hsa:7763 |
UCSCi | uc004aix.3, human |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF062072 Genomic DNA Translation: AAC61801.1 AF062346 mRNA Translation: AAC42601.1 AF062347 mRNA Translation: AAC42602.1 AK290849 mRNA Translation: BAF83538.1 AL135924 Genomic DNA No translation available. CH471089 Genomic DNA Translation: EAW62533.1 BC011018 mRNA Translation: AAH11018.1 BC027707 mRNA Translation: AAH27707.1 BC073131 mRNA Translation: AAH73131.1 |
CCDSi | CCDS6642.1 |
RefSeqi | NP_001095890.1, NM_001102420.2 NP_001095891.1, NM_001102421.2 NP_001265172.1, NM_001278243.1 NP_001265173.1, NM_001278244.1 NP_001265174.1, NM_001278245.1 NP_005998.1, NM_006007.3 |
3D structure databases
BMRBi | O76080 |
SMRi | O76080 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 113546, 22 interactors |
IntActi | O76080, 10 interactors |
MINTi | O76080 |
STRINGi | 9606.ENSP00000237937 |
PTM databases
iPTMneti | O76080 |
PhosphoSitePlusi | O76080 |
Genetic variation databases
BioMutai | ZFAND5 |
Proteomic databases
EPDi | O76080 |
jPOSTi | O76080 |
MassIVEi | O76080 |
MaxQBi | O76080 |
PaxDbi | O76080 |
PeptideAtlasi | O76080 |
PRIDEi | O76080 |
ProteomicsDBi | 50381 |
TopDownProteomicsi | O76080 |
Protocols and materials databases
Antibodypediai | 12564, 217 antibodies |
DNASUi | 7763 |
Genome annotation databases
Ensembli | ENST00000237937; ENSP00000237937; ENSG00000107372 ENST00000343431; ENSP00000350586; ENSG00000107372 ENST00000376960; ENSP00000366159; ENSG00000107372 ENST00000376962; ENSP00000366161; ENSG00000107372 |
GeneIDi | 7763 |
KEGGi | hsa:7763 |
UCSCi | uc004aix.3, human |
Organism-specific databases
CTDi | 7763 |
DisGeNETi | 7763 |
GeneCardsi | ZFAND5 |
HGNCi | HGNC:13008, ZFAND5 |
HPAi | ENSG00000107372, Tissue enhanced (skeletal) |
MIMi | 604761, gene |
neXtProti | NX_O76080 |
OpenTargetsi | ENSG00000107372 |
PharmGKBi | PA37587 |
VEuPathDBi | HostDB:ENSG00000107372.12 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG3173, Eukaryota |
GeneTreei | ENSGT00940000156165 |
HOGENOMi | CLU_057016_1_0_1 |
InParanoidi | O76080 |
OMAi | EMSISCE |
OrthoDBi | 1551371at2759 |
PhylomeDBi | O76080 |
TreeFami | TF313612 |
Enzyme and pathway databases
PathwayCommonsi | O76080 |
Miscellaneous databases
BioGRID-ORCSi | 7763, 34 hits in 965 CRISPR screens |
ChiTaRSi | ZFAND5, human |
GenomeRNAii | 7763 |
Pharosi | O76080, Tbio |
PROi | PR:O76080 |
RNActi | O76080, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000107372, Expressed in tibia and 241 other tissues |
ExpressionAtlasi | O76080, baseline and differential |
Genevisiblei | O76080, HS |
Family and domain databases
Gene3Di | 4.10.1110.10, 1 hit |
InterProi | View protein in InterPro IPR035896, AN1-like_Znf IPR002653, Znf_A20 IPR000058, Znf_AN1 |
Pfami | View protein in Pfam PF01754, zf-A20, 1 hit PF01428, zf-AN1, 1 hit |
SMARTi | View protein in SMART SM00259, ZnF_A20, 1 hit SM00154, ZnF_AN1, 1 hit |
SUPFAMi | SSF118310, SSF118310, 1 hit |
PROSITEi | View protein in PROSITE PS51036, ZF_A20, 1 hit PS51039, ZF_AN1, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | ZFAN5_HUMAN | |
Accessioni | O76080Primary (citable) accession number: O76080 Secondary accession number(s): A8K484 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 15, 2002 |
Last sequence update: | November 1, 1998 | |
Last modified: | April 7, 2021 | |
This is version 152 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Human chromosome 9
Human chromosome 9: entries, gene names and cross-references to MIM - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot