UniProtKB - O76071 (CIAO1_HUMAN)
Probable cytosolic iron-sulfur protein assembly protein CIAO1
CIAO1
Functioni
Key component of the cytosolic iron-sulfur protein assembly (CIA) complex, a multiprotein complex that mediates the incorporation of iron-sulfur cluster into extramitochondrial Fe/S proteins (PubMed:17937914, PubMed:23891004).
As a CIA complex component, interacts specifically with CIAO2A or CIAO2B and MMS19 to assist different branches of iron-sulfur protein assembly, depending of its interactors. The complex CIAO1:CIAO2B:MMS19 binds to and facilitates the assembly of most cytosolic-nuclear Fe/S proteins. CIAO1:CIAO2A specifically matures ACO1 and stabilizes IREB2 (PubMed:23891004).
Seems to specifically modulate the transactivation activity of WT1 (PubMed:9556563).
As part of the mitotic spindle-associated MMXD complex it may play a role in chromosome segregation (PubMed:20797633).
UniRule annotation4 PublicationsMiscellaneous
GO - Biological processi
- chromosome segregation Source: UniProtKB-KW
- iron-sulfur cluster assembly Source: UniProtKB
- positive regulation of cell population proliferation Source: ProtInc
- protein maturation by iron-sulfur cluster transfer Source: UniProtKB
- regulation of transcription by RNA polymerase II Source: ProtInc
Keywordsi
Biological process | Chromosome partition |
Enzyme and pathway databases
PathwayCommonsi | O76071 |
Reactomei | R-HSA-2564830, Cytosolic iron-sulfur cluster assembly |
SignaLinki | O76071 |
Names & Taxonomyi
Protein namesi | Recommended name: Probable cytosolic iron-sulfur protein assembly protein CIAO1UniRule annotationAlternative name(s): WD repeat-containing protein 39UniRule annotation |
Gene namesi | |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:14280, CIAO1 |
MIMi | 604333, gene |
neXtProti | NX_O76071 |
VEuPathDBi | HostDB:ENSG00000144021 |
Subcellular locationi
Cytoplasm and Cytosol
- Cytoplasm 1 Publication
Cytoskeleton
- MMXD complex Source: UniProtKB
Cytosol
- CIA complex Source: UniProtKB
Other locations
- cytoplasm Source: UniProtKB
Keywords - Cellular componenti
CytoplasmPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 87 – 89 | IWK → AAA: Does not affect binding to HSC20. 1 Publication | 3 | |
Mutagenesisi | 176 – 178 | LYR → AAA: Abolishes binding to HSC20. 1 Publication | 3 |
Organism-specific databases
DisGeNETi | 9391 |
OpenTargetsi | ENSG00000144021 |
PharmGKBi | PA162382269 |
Miscellaneous databases
Pharosi | O76071, Tbio |
Genetic variation databases
BioMutai | CIAO1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000051389 | 1 – 339 | Probable cytosolic iron-sulfur protein assembly protein CIAO1Add BLAST | 339 |
Proteomic databases
EPDi | O76071 |
jPOSTi | O76071 |
MassIVEi | O76071 |
MaxQBi | O76071 |
PaxDbi | O76071 |
PeptideAtlasi | O76071 |
PRIDEi | O76071 |
ProteomicsDBi | 50373 |
TopDownProteomicsi | O76071 |
PTM databases
GlyGeni | O76071, 1 site, 1 O-linked glycan (1 site) |
iPTMneti | O76071 |
MetOSitei | O76071 |
PhosphoSitePlusi | O76071 |
SwissPalmi | O76071 |
Expressioni
Gene expression databases
Bgeei | ENSG00000144021, Expressed in right adrenal gland and 232 other tissues |
Genevisiblei | O76071, HS |
Organism-specific databases
HPAi | ENSG00000144021, Low tissue specificity |
Interactioni
Subunit structurei
Component of the CIA complex (PubMed:22678361, PubMed:22678362, PubMed:23585563).
Interacts with CIAO2A and forms a complex with CIAO2B and MMS19; the interactions with CIAO2A and CIAO2B are mutually exclusive (PubMed:22683786, PubMed:23891004, PubMed:23585563).
Interacts with CHD1L, ERCC2, IREB2 and POLD1 (PubMed:23891004).
Component of the MMXD complex, which includes CIAO1, ERCC2, CIAO2B, MMS19 and SLC25A5 (PubMed:20797633).
Interacts with WT1 (PubMed:9556563).
Interacts with CIAO3 (PubMed:23585563).
Interacts (via LYR motif) with HSC20 (PubMed:29309586).
UniRule annotation8 PublicationsBinary interactionsi
O76071
Protein-protein interaction databases
BioGRIDi | 114791, 202 interactors |
CORUMi | O76071 |
IntActi | O76071, 86 interactors |
MINTi | O76071 |
STRINGi | 9606.ENSP00000418287 |
Miscellaneous databases
RNActi | O76071, protein |
Structurei
Secondary structure
3D structure databases
AlphaFoldDBi | O76071 |
SMRi | O76071 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | O76071 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Repeati | 14 – 53 | WD 1Add BLAST | 40 | |
Repeati | 59 – 98 | WD 2Add BLAST | 40 | |
Repeati | 103 – 142 | WD 3Add BLAST | 40 | |
Repeati | 148 – 187 | WD 4Add BLAST | 40 | |
Repeati | 192 – 231 | WD 5Add BLAST | 40 | |
Repeati | 250 – 289 | WD 6Add BLAST | 40 | |
Repeati | 301 – 339 | WD 7Add BLAST | 39 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 176 – 178 | LYR motif; required for interaction with HSC201 Publication | 3 |
Sequence similaritiesi
Keywords - Domaini
Repeat, WD repeatPhylogenomic databases
eggNOGi | KOG0645, Eukaryota |
GeneTreei | ENSGT00940000158670 |
HOGENOMi | CLU_000288_57_8_1 |
InParanoidi | O76071 |
OMAi | AIWSKSS |
OrthoDBi | 793298at2759 |
PhylomeDBi | O76071 |
TreeFami | TF318181 |
Family and domain databases
Gene3Di | 2.130.10.10, 1 hit |
HAMAPi | MF_03037, ciao1, 1 hit |
InterProi | View protein in InterPro IPR028608, CIAO1/Cia1 IPR015943, WD40/YVTN_repeat-like_dom_sf IPR001680, WD40_repeat IPR019775, WD40_repeat_CS IPR036322, WD40_repeat_dom_sf |
PANTHERi | PTHR19920, PTHR19920, 1 hit |
Pfami | View protein in Pfam PF00400, WD40, 6 hits |
SMARTi | View protein in SMART SM00320, WD40, 7 hits |
SUPFAMi | SSF50978, SSF50978, 1 hit |
PROSITEi | View protein in PROSITE PS00678, WD_REPEATS_1, 1 hit PS50082, WD_REPEATS_2, 6 hits PS50294, WD_REPEATS_REGION, 1 hit |
i Sequence
Sequence statusi: Complete.
10 20 30 40 50
MKDSLVLLGR VPAHPDSRCW FLAWNPAGTL LASCGGDRRI RIWGTEGDSW
60 70 80 90 100
ICKSVLSEGH QRTVRKVAWS PCGNYLASAS FDATTCIWKK NQDDFECVTT
110 120 130 140 150
LEGHENEVKS VAWAPSGNLL ATCSRDKSVW VWEVDEEDEY ECVSVLNSHT
160 170 180 190 200
QDVKHVVWHP SQELLASASY DDTVKLYREE EDDWVCCATL EGHESTVWSL
210 220 230 240 250
AFDPSGQRLA SCSDDRTVRI WRQYLPGNEQ GVACSGSDPS WKCICTLSGF
260 270 280 290 300
HSRTIYDIAW CQLTGALATA CGDDAIRVFQ EDPNSDPQQP TFSLTAHLHQ
310 320 330
AHSQDVNCVA WNPKEPGLLA SCSDDGEVAF WKYQRPEGL
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 243 | C → G in BAD96977 (Ref. 5) Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U63810 mRNA Translation: AAC24948.1 EF011618 mRNA Translation: ABK41108.1 AC004020 Genomic DNA Translation: AAC23493.1 CR456802 mRNA Translation: CAG33083.1 AK223257 mRNA Translation: BAD96977.1 BC001395 mRNA Translation: AAH01395.1 BC032812 mRNA Translation: AAH32812.1 |
CCDSi | CCDS2019.1 |
RefSeqi | NP_004795.1, NM_004804.2 |
Genome annotation databases
Ensembli | ENST00000488633.2; ENSP00000418287.1; ENSG00000144021.3 |
GeneIDi | 9391 |
KEGGi | hsa:9391 |
MANE-Selecti | ENST00000488633.2; ENSP00000418287.1; NM_004804.3; NP_004795.1 |
UCSCi | uc002svs.4, human |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U63810 mRNA Translation: AAC24948.1 EF011618 mRNA Translation: ABK41108.1 AC004020 Genomic DNA Translation: AAC23493.1 CR456802 mRNA Translation: CAG33083.1 AK223257 mRNA Translation: BAD96977.1 BC001395 mRNA Translation: AAH01395.1 BC032812 mRNA Translation: AAH32812.1 |
CCDSi | CCDS2019.1 |
RefSeqi | NP_004795.1, NM_004804.2 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
3FM0 | X-ray | 1.70 | A | 1-339 | [»] | |
AlphaFoldDBi | O76071 | |||||
SMRi | O76071 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 114791, 202 interactors |
CORUMi | O76071 |
IntActi | O76071, 86 interactors |
MINTi | O76071 |
STRINGi | 9606.ENSP00000418287 |
PTM databases
GlyGeni | O76071, 1 site, 1 O-linked glycan (1 site) |
iPTMneti | O76071 |
MetOSitei | O76071 |
PhosphoSitePlusi | O76071 |
SwissPalmi | O76071 |
Genetic variation databases
BioMutai | CIAO1 |
Proteomic databases
EPDi | O76071 |
jPOSTi | O76071 |
MassIVEi | O76071 |
MaxQBi | O76071 |
PaxDbi | O76071 |
PeptideAtlasi | O76071 |
PRIDEi | O76071 |
ProteomicsDBi | 50373 |
TopDownProteomicsi | O76071 |
Protocols and materials databases
ABCDi | O76071, 1 sequenced antibody |
Antibodypediai | 17416, 247 antibodies from 29 providers |
DNASUi | 9391 |
Genome annotation databases
Ensembli | ENST00000488633.2; ENSP00000418287.1; ENSG00000144021.3 |
GeneIDi | 9391 |
KEGGi | hsa:9391 |
MANE-Selecti | ENST00000488633.2; ENSP00000418287.1; NM_004804.3; NP_004795.1 |
UCSCi | uc002svs.4, human |
Organism-specific databases
CTDi | 9391 |
DisGeNETi | 9391 |
GeneCardsi | CIAO1 |
HGNCi | HGNC:14280, CIAO1 |
HPAi | ENSG00000144021, Low tissue specificity |
MIMi | 604333, gene |
neXtProti | NX_O76071 |
OpenTargetsi | ENSG00000144021 |
PharmGKBi | PA162382269 |
VEuPathDBi | HostDB:ENSG00000144021 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG0645, Eukaryota |
GeneTreei | ENSGT00940000158670 |
HOGENOMi | CLU_000288_57_8_1 |
InParanoidi | O76071 |
OMAi | AIWSKSS |
OrthoDBi | 793298at2759 |
PhylomeDBi | O76071 |
TreeFami | TF318181 |
Enzyme and pathway databases
PathwayCommonsi | O76071 |
Reactomei | R-HSA-2564830, Cytosolic iron-sulfur cluster assembly |
SignaLinki | O76071 |
Miscellaneous databases
BioGRID-ORCSi | 9391, 670 hits in 1082 CRISPR screens |
ChiTaRSi | CIAO1, human |
EvolutionaryTracei | O76071 |
GeneWikii | CIAO1 |
GenomeRNAii | 9391 |
Pharosi | O76071, Tbio |
PROi | PR:O76071 |
RNActi | O76071, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000144021, Expressed in right adrenal gland and 232 other tissues |
Genevisiblei | O76071, HS |
Family and domain databases
Gene3Di | 2.130.10.10, 1 hit |
HAMAPi | MF_03037, ciao1, 1 hit |
InterProi | View protein in InterPro IPR028608, CIAO1/Cia1 IPR015943, WD40/YVTN_repeat-like_dom_sf IPR001680, WD40_repeat IPR019775, WD40_repeat_CS IPR036322, WD40_repeat_dom_sf |
PANTHERi | PTHR19920, PTHR19920, 1 hit |
Pfami | View protein in Pfam PF00400, WD40, 6 hits |
SMARTi | View protein in SMART SM00320, WD40, 7 hits |
SUPFAMi | SSF50978, SSF50978, 1 hit |
PROSITEi | View protein in PROSITE PS00678, WD_REPEATS_1, 1 hit PS50082, WD_REPEATS_2, 6 hits PS50294, WD_REPEATS_REGION, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | CIAO1_HUMAN | |
Accessioni | O76071Primary (citable) accession number: O76071 Secondary accession number(s): A0MNN9, Q53FM5 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | January 11, 2001 |
Last sequence update: | November 1, 1998 | |
Last modified: | May 25, 2022 | |
This is version 190 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Human chromosome 2
Human chromosome 2: entries, gene names and cross-references to MIM - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families