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Protein

Carboxypeptidase D

Gene

CPD

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalytic activityi

Releases C-terminal Arg and Lys from polypeptides.

Cofactori

Zn2+By similarityNote: Binds 2 Zn2+ ions per subunit.By similarity

pH dependencei

Optimum pH is 6.0-6.5.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi139Zinc 1; catalyticBy similarity1
Metal bindingi142Zinc 1; catalyticBy similarity1
Metal bindingi257Zinc 1; catalyticBy similarity1
Metal bindingi564Zinc 2; catalyticBy similarity1
Metal bindingi567Zinc 2; catalyticBy similarity1
Metal bindingi671Zinc 2; catalyticBy similarity1
Active sitei762Proton donor/acceptorBy similarity1

GO - Molecular functioni

  • metallocarboxypeptidase activity Source: GO_Central
  • serine-type carboxypeptidase activity Source: ProtInc
  • zinc ion binding Source: InterPro

GO - Biological processi

Keywordsi

Molecular functionCarboxypeptidase, Hydrolase, Metalloprotease, Protease
LigandMetal-binding, Zinc

Enzyme and pathway databases

BRENDAi3.4.17.22 2681
ReactomeiR-HSA-432722 Golgi Associated Vesicle Biogenesis

Protein family/group databases

MEROPSiM14.011

Names & Taxonomyi

Protein namesi
Recommended name:
Carboxypeptidase D (EC:3.4.17.22)
Alternative name(s):
Metallocarboxypeptidase D
gp180
Gene namesi
Name:CPD
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 17

Organism-specific databases

EuPathDBiHostDB:ENSG00000108582.11
HGNCiHGNC:2301 CPD
MIMi603102 gene
neXtProtiNX_O75976

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini32 – 1299ExtracellularSequence analysisAdd BLAST1268
Transmembranei1300 – 1320HelicalSequence analysisAdd BLAST21
Topological domaini1321 – 1380CytoplasmicSequence analysisAdd BLAST60

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Organism-specific databases

DisGeNETi1362
OpenTargetsiENSG00000108582
PharmGKBiPA26823

Chemistry databases

DrugBankiDB04489 Guanidinoethylmercaptosuccinic acid

Polymorphism and mutation databases

BioMutaiCPD

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 31Sequence analysisAdd BLAST31
ChainiPRO_000000440132 – 1380Carboxypeptidase DAdd BLAST1349

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi172N-linked (GlcNAc...) asparagine1 Publication1
Glycosylationi217N-linked (GlcNAc...) asparagineSequence analysis1
Modified residuei265PhosphotyrosineBy similarity1
Modified residuei270PhosphoserineBy similarity1
Glycosylationi399N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi410N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi429N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi522N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi626N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi811N-linked (GlcNAc...) asparagine2 Publications1
Glycosylationi855N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi867N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi879N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi955N-linked (GlcNAc...) asparagine2 Publications1
Glycosylationi978N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi1070N-linked (GlcNAc...) asparagine1 Publication1
Glycosylationi1142N-linked (GlcNAc...) asparagineSequence analysis1
Lipidationi1317S-palmitoyl cysteine1 Publication1
Lipidationi1321S-palmitoyl cysteine1 Publication1
Lipidationi1323S-palmitoyl cysteine1 Publication1
Modified residuei1358PhosphoserineCombined sources1
Modified residuei1361PhosphoserineCombined sources1
Modified residuei1368PhosphothreonineCombined sources1
Modified residuei1370PhosphothreonineCombined sources1

Keywords - PTMi

Glycoprotein, Lipoprotein, Palmitate, Phosphoprotein

Proteomic databases

EPDiO75976
MaxQBiO75976
PaxDbiO75976
PeptideAtlasiO75976
PRIDEiO75976
ProteomicsDBi50336

PTM databases

CarbonylDBiO75976
GlyConnecti1066
iPTMnetiO75976
PhosphoSitePlusiO75976
SwissPalmiO75976

Expressioni

Tissue specificityi

Highly expressed in placenta, pancreas and hepatoma cells. Lower levels found in skeletal muscle, heart and colon carcinoma and melanoma cell lines.

Gene expression databases

BgeeiENSG00000108582 Expressed in 233 organ(s), highest expression level in parotid gland
CleanExiHS_CPD
ExpressionAtlasiO75976 baseline and differential
GenevisibleiO75976 HS

Organism-specific databases

HPAiHPA052796
HPA055465

Interactioni

Protein-protein interaction databases

BioGridi107754, 21 interactors
IntActiO75976, 12 interactors
MINTiO75976
STRINGi9606.ENSP00000225719

Structurei

Secondary structure

11380
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliO75976
SMRiO75976
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni32 – 493Carboxypeptidase-like 1Add BLAST462
Regioni494 – 897Carboxypeptidase-like 2Add BLAST404
Regioni898 – 1299Carboxypeptidase-like 3Add BLAST402

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi162 – 164Cell attachment siteSequence analysis3

Domaini

There are 3 carboxypeptidase-like domains. Only the first two domains seem to have kept a catalytic activity.

Sequence similaritiesi

Belongs to the peptidase M14 family.Curated

Keywords - Domaini

Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG2649 Eukaryota
ENOG410XX0H LUCA
GeneTreeiENSGT00760000119124
HOGENOMiHOG000046445
HOVERGENiHBG006932
InParanoidiO75976
KOiK07752
OMAiDFRHHHF
OrthoDBiEOG091G06A9
PhylomeDBiO75976
TreeFamiTF315592

Family and domain databases

CDDicd03868 M14_CPD_I, 1 hit
cd03863 M14_CPD_II, 1 hit
cd06245 M14_CPD_III, 1 hit
InterProiView protein in InterPro
IPR008969 CarboxyPept-like_regulatory
IPR034241 M14_CPD_I
IPR034224 M14_CPD_II
IPR033848 M14_CPD_III
IPR015567 Pept_M14B_carboxypept_D2
IPR000834 Peptidase_M14
PANTHERiPTHR11532:SF73 PTHR11532:SF73, 3 hits
PfamiView protein in Pfam
PF00246 Peptidase_M14, 3 hits
PRINTSiPR00765 CRBOXYPTASEA
SMARTiView protein in SMART
SM00631 Zn_pept, 3 hits
SUPFAMiSSF49464 SSF49464, 3 hits
PROSITEiView protein in PROSITE
PS00132 CARBOXYPEPT_ZN_1, 2 hits
PS00133 CARBOXYPEPT_ZN_2, 2 hits

Sequences (2+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 2 described isoforms and 3 potential isoforms that are computationally mapped.Show allAlign All

Isoform 1 (identifier: O75976-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MASGRDERPP WRLGRLLLLM CLLLLGSSAR AAHIKKAEAT TTTTSAGAEA
60 70 80 90 100
AEGQFDRYYH EEELESALRE AAAAGLPGLA RLFSIGRSVE GRPLWVLRLT
110 120 130 140 150
AGLGSLIPEG DAGPDAAGPD AAGPLLPGRP QVKLVGNMHG DETVSRQVLI
160 170 180 190 200
YLARELAAGY RRGDPRLVRL LNTTDVYLLP SLNPDGFERA REGDCGFGDG
210 220 230 240 250
GPSGASGRDN SRGRDLNRSF PDQFSTGEPP ALDEVPEVRA LIEWIRRNKF
260 270 280 290 300
VLSGNLHGGS VVASYPFDDS PEHKATGIYS KTSDDEVFKY LAKAYASNHP
310 320 330 340 350
IMKTGEPHCP GDEDETFKDG ITNGAHWYDV EGGMQDYNYV WANCFEITLE
360 370 380 390 400
LSCCKYPPAS QLRQEWENNR ESLITLIEKV HIGVKGFVKD SITGSGLENA
410 420 430 440 450
TISVAGINHN ITTGRFGDFY RLLVPGTYNL TVVLTGYMPL TVTNVVVKEG
460 470 480 490 500
PATEVDFSLR PTVTSVIPDT TEAVSTASTV AIPNILSGTS SSYQPIQPKD
510 520 530 540 550
FHHHHFPDME IFLRRFANEY PNITRLYSLG KSVESRELYV MEISDNPGVH
560 570 580 590 600
EPGEPEFKYI GNMHGNEVVG RELLLNLIEY LCKNFGTDPE VTDLVHNTRI
610 620 630 640 650
HLMPSMNPDG YEKSQEGDSI SVIGRNNSNN FDLNRNFPDQ FVQITDPTQP
660 670 680 690 700
ETIAVMSWMK SYPFVLSANL HGGSLVVNYP FDDDEQGLAT YSKSPDDAVF
710 720 730 740 750
QQIALSYSKE NSQMFQGRPC KNMYPNEYFP HGITNGASWY NVPGGMQDWN
760 770 780 790 800
YLQTNCFEVT IELGCVKYPL EKELPNFWEQ NRRSLIQFMK QVHQGVRGFV
810 820 830 840 850
LDATDGRGIL NATISVAEIN HPVTTYKTGD YWRLLVPGTY KITASARGYN
860 870 880 890 900
PVTKNVTVKS EGAIQVNFTL VRSSTDSNNE SKKGKGASSS TNDASDPTTK
910 920 930 940 950
EFETLIKDLS AENGLESLML RSSSNLALAL YRYHSYKDLS EFLRGLVMNY
960 970 980 990 1000
PHITNLTNLG QSTEYRHIWS LEISNKPNVS EPEEPKIRFV AGIHGNAPVG
1010 1020 1030 1040 1050
TELLLALAEF LCLNYKKNPA VTQLVDRTRI VIVPSLNPDG RERAQEKDCT
1060 1070 1080 1090 1100
SKIGQTNARG KDLDTDFTNN ASQPETKAII ENLIQKQDFS LSVALDGGSM
1110 1120 1130 1140 1150
LVTYPYDKPV QTVENKETLK HLASLYANNH PSMHMGQPSC PNKSDENIPG
1160 1170 1180 1190 1200
GVMRGAEWHS HLGSMKDYSV TYGHCPEITV YTSCCYFPSA ARLPSLWADN
1210 1220 1230 1240 1250
KRSLLSMLVE VHKGVHGFVK DKTGKPISKA VIVLNEGIKV QTKEGGYFHV
1260 1270 1280 1290 1300
LLAPGVHNII AIADGYQQQH SQVFVHHDAA SSVVIVFDTD NRIFGLPREL
1310 1320 1330 1340 1350
VVTVSGATMS ALILTACIIW CICSIKSNRH KDGFHRLRQH HDEYEDEIRM
1360 1370 1380
MSTGSKKSLL SHEFQDETDT EEETLYSSKH
Length:1,380
Mass (Da):152,931
Last modified:October 3, 2006 - v2
Checksum:i567EC1F0B4B7A0C8
GO
Isoform 2 (identifier: O75976-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-2: MA → MR
     3-249: Missing.

Note: No experimental confirmation available.
Show »
Length:1,133
Mass (Da):126,487
Checksum:iD253BDC1BA9B56B6
GO

Computationally mapped potential isoform sequencesi

There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
J3QQJ4J3QQJ4_HUMAN
Carboxypeptidase D
CPD
184Annotation score:
J3QRJ9J3QRJ9_HUMAN
Carboxypeptidase D
CPD
82Annotation score:
K7ELF0K7ELF0_HUMAN
Carboxypeptidase D
CPD
40Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti863A → V in BAH14780 (PubMed:14702039).Curated1
Sequence conflicti896D → V (PubMed:9714835).Curated1
Sequence conflicti896D → V (PubMed:9355738).Curated1
Sequence conflicti948M → I in BAH14780 (PubMed:14702039).Curated1
Sequence conflicti1336R → W in BAH13725 (PubMed:14702039).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_02777136K → E1 PublicationCorresponds to variant dbSNP:rs17857300Ensembl.1
Natural variantiVAR_027772454E → G1 PublicationCorresponds to variant dbSNP:rs17857301Ensembl.1
Natural variantiVAR_027773505H → N1 PublicationCorresponds to variant dbSNP:rs17854355Ensembl.1
Natural variantiVAR_027774899T → I. Corresponds to variant dbSNP:rs1860543Ensembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0458331 – 2MA → MR in isoform 2. 1 Publication2
Alternative sequenceiVSP_0458343 – 249Missing in isoform 2. 1 PublicationAdd BLAST247

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D85390 mRNA Translation: BAA33370.1
U65090 mRNA Translation: AAC51775.2
AK302497 mRNA Translation: BAH13725.1
AK316409 mRNA Translation: BAH14780.1
AC006050 Genomic DNA No translation available.
AC090685 Genomic DNA No translation available.
BC045549 mRNA Translation: AAH45549.1
BC045624 mRNA Translation: AAH45624.1
BC051702 mRNA Translation: AAH51702.1
CCDSiCCDS11257.1 [O75976-1]
CCDS56025.1 [O75976-2]
RefSeqiNP_001186704.1, NM_001199775.1 [O75976-2]
NP_001295.2, NM_001304.4 [O75976-1]
UniGeneiHs.446079

Genome annotation databases

EnsembliENST00000225719; ENSP00000225719; ENSG00000108582 [O75976-1]
ENST00000543464; ENSP00000444443; ENSG00000108582 [O75976-2]
GeneIDi1362
KEGGihsa:1362
UCSCiuc002hfb.3 human [O75976-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D85390 mRNA Translation: BAA33370.1
U65090 mRNA Translation: AAC51775.2
AK302497 mRNA Translation: BAH13725.1
AK316409 mRNA Translation: BAH14780.1
AC006050 Genomic DNA No translation available.
AC090685 Genomic DNA No translation available.
BC045549 mRNA Translation: AAH45549.1
BC045624 mRNA Translation: AAH45624.1
BC051702 mRNA Translation: AAH51702.1
CCDSiCCDS11257.1 [O75976-1]
CCDS56025.1 [O75976-2]
RefSeqiNP_001186704.1, NM_001199775.1 [O75976-2]
NP_001295.2, NM_001304.4 [O75976-1]
UniGeneiHs.446079

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5AQ0X-ray0.95A/B383-461[»]
ProteinModelPortaliO75976
SMRiO75976
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi107754, 21 interactors
IntActiO75976, 12 interactors
MINTiO75976
STRINGi9606.ENSP00000225719

Chemistry databases

DrugBankiDB04489 Guanidinoethylmercaptosuccinic acid

Protein family/group databases

MEROPSiM14.011

PTM databases

CarbonylDBiO75976
GlyConnecti1066
iPTMnetiO75976
PhosphoSitePlusiO75976
SwissPalmiO75976

Polymorphism and mutation databases

BioMutaiCPD

Proteomic databases

EPDiO75976
MaxQBiO75976
PaxDbiO75976
PeptideAtlasiO75976
PRIDEiO75976
ProteomicsDBi50336

Protocols and materials databases

DNASUi1362
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000225719; ENSP00000225719; ENSG00000108582 [O75976-1]
ENST00000543464; ENSP00000444443; ENSG00000108582 [O75976-2]
GeneIDi1362
KEGGihsa:1362
UCSCiuc002hfb.3 human [O75976-1]

Organism-specific databases

CTDi1362
DisGeNETi1362
EuPathDBiHostDB:ENSG00000108582.11
GeneCardsiCPD
HGNCiHGNC:2301 CPD
HPAiHPA052796
HPA055465
MIMi603102 gene
neXtProtiNX_O75976
OpenTargetsiENSG00000108582
PharmGKBiPA26823
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG2649 Eukaryota
ENOG410XX0H LUCA
GeneTreeiENSGT00760000119124
HOGENOMiHOG000046445
HOVERGENiHBG006932
InParanoidiO75976
KOiK07752
OMAiDFRHHHF
OrthoDBiEOG091G06A9
PhylomeDBiO75976
TreeFamiTF315592

Enzyme and pathway databases

BRENDAi3.4.17.22 2681
ReactomeiR-HSA-432722 Golgi Associated Vesicle Biogenesis

Miscellaneous databases

ChiTaRSiCPD human
GeneWikiiCPD_(gene)
GenomeRNAii1362
PROiPR:O75976
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000108582 Expressed in 233 organ(s), highest expression level in parotid gland
CleanExiHS_CPD
ExpressionAtlasiO75976 baseline and differential
GenevisibleiO75976 HS

Family and domain databases

CDDicd03868 M14_CPD_I, 1 hit
cd03863 M14_CPD_II, 1 hit
cd06245 M14_CPD_III, 1 hit
InterProiView protein in InterPro
IPR008969 CarboxyPept-like_regulatory
IPR034241 M14_CPD_I
IPR034224 M14_CPD_II
IPR033848 M14_CPD_III
IPR015567 Pept_M14B_carboxypept_D2
IPR000834 Peptidase_M14
PANTHERiPTHR11532:SF73 PTHR11532:SF73, 3 hits
PfamiView protein in Pfam
PF00246 Peptidase_M14, 3 hits
PRINTSiPR00765 CRBOXYPTASEA
SMARTiView protein in SMART
SM00631 Zn_pept, 3 hits
SUPFAMiSSF49464 SSF49464, 3 hits
PROSITEiView protein in PROSITE
PS00132 CARBOXYPEPT_ZN_1, 2 hits
PS00133 CARBOXYPEPT_ZN_2, 2 hits
ProtoNetiSearch...

Entry informationi

Entry nameiCBPD_HUMAN
AccessioniPrimary (citable) accession number: O75976
Secondary accession number(s): B7Z7T9
, B7ZAU4, F5GZH6, O15377, Q86SH9, Q86XE6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 15, 2002
Last sequence update: October 3, 2006
Last modified: November 7, 2018
This is version 169 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Peptidase families
    Classification of peptidase families and list of entries
  4. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  5. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  6. Human chromosome 17
    Human chromosome 17: entries, gene names and cross-references to MIM
  7. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
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