UniProtKB - O75969 (AKAP3_HUMAN)
Protein
A-kinase anchor protein 3
Gene
AKAP3
Organism
Homo sapiens (Human)
Status
Functioni
May function as a regulator of both motility- and head-associated functions such as capacitation and the acrosome reaction.
GO - Molecular functioni
- protein kinase A binding Source: GO_Central
GO - Biological processi
- acrosome reaction Source: ProtInc
- blastocyst hatching Source: Ensembl
- protein localization Source: GO_Central
- single fertilization Source: ProtInc
- transmembrane receptor protein serine/threonine kinase signaling pathway Source: Ensembl
Enzyme and pathway databases
PathwayCommonsi | O75969 |
Names & Taxonomyi
Protein namesi | Recommended name: A-kinase anchor protein 3Short name: AKAP-3 Alternative name(s): A-kinase anchor protein 110 kDa Short name: AKAP 110 Cancer/testis antigen 82 Short name: CT82 Fibrous sheath protein of 95 kDa Short name: FSP95 Fibrousheathin I Fibrousheathin-1 Protein kinase A-anchoring protein 3 Short name: PRKA3 Sperm oocyte-binding protein |
Gene namesi | Name:AKAP3 Synonyms:AKAP110, SOB1 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
EuPathDBi | HostDB:ENSG00000111254.7 |
HGNCi | HGNC:373, AKAP3 |
MIMi | 604689, gene |
neXtProti | NX_O75969 |
Subcellular locationi
Other locations
- acrosome By similarity
Note: Ribs of the fibrous sheath in the principal piece of the sperm tail. Dorsal margin of the acrosomal segment.
Nucleus
- nucleus Source: UniProtKB
Other locations
- acrosomal vesicle Source: UniProtKB-SubCell
- cytoplasm Source: GO_Central
- motile cilium Source: GO_Central
- sperm fibrous sheath Source: Ensembl
- sperm midpiece Source: UniProtKB
- sperm principal piece Source: UniProtKB
Keywords - Cellular componenti
Cytoplasmic vesiclePathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 131 | L → P: Abolishes interaction with ROPN1. 1 Publication | 1 |
Organism-specific databases
DisGeNETi | 10566 |
OpenTargetsi | ENSG00000111254 |
PharmGKBi | PA24667 |
Miscellaneous databases
Pharosi | O75969, Tbio |
Polymorphism and mutation databases
BioMutai | AKAP3 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000064526 | 1 – 853 | A-kinase anchor protein 3Add BLAST | 853 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 205 | PhosphoserineCombined sources | 1 | |
Modified residuei | 208 | PhosphoserineCombined sources | 1 | |
Modified residuei | 403 | PhosphoserineCombined sources | 1 | |
Modified residuei | 404 | PhosphotyrosineCombined sources | 1 | |
Modified residuei | 635 | PhosphoserineCombined sources | 1 | |
Modified residuei | 636 | PhosphoserineCombined sources | 1 |
Post-translational modificationi
Phosphorylated on tyrosine residues.
Keywords - PTMi
PhosphoproteinProteomic databases
EPDi | O75969 |
jPOSTi | O75969 |
MassIVEi | O75969 |
PaxDbi | O75969 |
PeptideAtlasi | O75969 |
PRIDEi | O75969 |
ProteomicsDBi | 50329 |
PTM databases
iPTMneti | O75969 |
PhosphoSitePlusi | O75969 |
Expressioni
Tissue specificityi
Testis specific; only expressed in spermatids.
Gene expression databases
Bgeei | ENSG00000111254, Expressed in testis and 123 other tissues |
ExpressionAtlasi | O75969, baseline and differential |
Genevisiblei | O75969, HS |
Organism-specific databases
HPAi | ENSG00000111254, Tissue enriched (testis) |
Interactioni
Subunit structurei
Binary interactionsi
Hide detailsO75969
With | #Exp. | IntAct |
---|---|---|
BRAP [Q7Z569] | 2 | EBI-9033101,EBI-349900 |
PRKAR2A [P13861] | 2 | EBI-9033101,EBI-2556122 |
ROPN1B [Q9BZX4] | 2 | EBI-9033101,EBI-9033148 |
GO - Molecular functioni
- protein kinase A binding Source: GO_Central
Protein-protein interaction databases
BioGRIDi | 115817, 8 interactors |
IntActi | O75969, 5 interactors |
STRINGi | 9606.ENSP00000228850 |
Miscellaneous databases
RNActi | O75969, protein |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 124 – 137 | PKA-RII subunit binding domainAdd BLAST | 14 |
Domaini
RII-binding site, predicted to form an amphipathic helix, could participate in protein-protein interactions with a complementary surface on the R-subunit dimer.
Sequence similaritiesi
Belongs to the AKAP110 family.Curated
Phylogenomic databases
eggNOGi | ENOG502SM7F, Eukaryota |
GeneTreei | ENSGT00940000153313 |
HOGENOMi | CLU_017072_0_0_1 |
InParanoidi | O75969 |
OMAi | DWKMDTS |
OrthoDBi | 221175at2759 |
PhylomeDBi | O75969 |
TreeFami | TF105403 |
Family and domain databases
InterProi | View protein in InterPro IPR020799, AKAP_110 IPR018292, AKAP_110_C IPR018459, RII_binding_1 IPR008382, SPHK1-interactor_AKAP_110 |
PANTHERi | PTHR10226, PTHR10226, 1 hit |
Pfami | View protein in Pfam PF05716, AKAP_110, 1 hit PF10522, RII_binding_1, 1 hit |
SMARTi | View protein in SMART SM00807, AKAP_110, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 2 potential isoforms that are computationally mapped.Show allAlign All
O75969-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSEKVDWLQS QNGVCKVDVY SPGDNQAQDW KMDTSTDPVR VLSWLRRDLE
60 70 80 90 100
KSTAEFQDVR FKPGESFGGE TSNSGDPHKG FSVDYYNTTT KGTPERLHFE
110 120 130 140 150
MTHKEIPCQG PRAQLGNGSS VDEVSFYANR LTNLVIAMAR KEINEKIDGS
160 170 180 190 200
ENKCVYQSLY MGNEPTPTKS LSKIASELVN ETVSACSRNA APDKAPGSGD
210 220 230 240 250
RVSGSSQSPP NLKYKSTLKI KESTKERQGP DDKPPSKKSF FYKEVFESRN
260 270 280 290 300
GDYAREGGRF FPRERKRFRG QERPDDFTAS VSEGIMTYAN SVVSDMMVSI
310 320 330 340 350
MKTLKIQVKD TTIATILLKK VLLKHAKEVV SDLIDSFLRN LHSVTGTLMT
360 370 380 390 400
DTQFVSAVKR TVFSHGSQKA TDIMDAMLRK LYNVMFAKKV PEHVRKAQDK
410 420 430 440 450
AESYSLISMK GMGDPKNRNV NFAMKSETKL REKMYSEPKS EEETCAKTLG
460 470 480 490 500
EHIIKEGLTL WHKTQQKECK SLGFQHAAFE APNTQRKPAS DISFEYPEDI
510 520 530 540 550
GNLSLPPYPP EKPENFMYDS DSWAEDLIVS ALLLIQYHLA QGGRRDARSF
560 570 580 590 600
VEAAGTTNFP ANEPPVAPDE SCLKSAPIVG DQEQAEKKDL RSVFFNFIRN
610 620 630 640 650
LLSETIFKRD QSPEPKVPEQ PVKEDRKLCE RPLASSPPRL YEDDETPGAL
660 670 680 690 700
SGLTKMAVSQ IDGHMSGQMV EHLMNSVMKL CVIIAKSCDA SLAELGDDKS
710 720 730 740 750
GDASRLTSAF PDSLYECLPA KGTGSAEAVL QNAYQAIHNE MRGTSGQPPE
760 770 780 790 800
GCAAPTVIVS NHNLTDTVQN KQLQAVLQWV AASELNVPIL YFAGDDEGIQ
810 820 830 840 850
EKLLQLSAAA VDKGCSVGEV LQSVLRYEKE RQLNEAVGNV TPLQLLDWLM
VNL
Computationally mapped potential isoform sequencesi
There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketF5H2S4 | F5H2S4_HUMAN | A-kinase anchor protein 3 | AKAP3 | 96 | Annotation score: | ||
F5H7P4 | F5H7P4_HUMAN | A-kinase anchor protein 3 | AKAP3 | 161 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 272 | E → D in AAD21218 (PubMed:10334916).Curated | 1 | |
Sequence conflicti | 282 | S → G in AAC35854 (PubMed:10529264).Curated | 1 | |
Sequence conflicti | 402 | E → V in AAC35854 (PubMed:10529264).Curated | 1 | |
Sequence conflicti | 467 | K → N in AAC35854 (PubMed:10529264).Curated | 1 | |
Sequence conflicti | 597 | F → S AA sequence (PubMed:10529264).Curated | 1 | |
Sequence conflicti | 700 | S → L in AAC35854 (PubMed:10529264).Curated | 1 |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_055488 | 118 | G → E4 PublicationsCorresponds to variant dbSNP:rs2072355Ensembl. | 1 | |
Natural variantiVAR_060730 | 464 | T → S3 PublicationsCorresponds to variant dbSNP:rs11063266Ensembl. | 1 | |
Natural variantiVAR_055489 | 500 | I → T1 PublicationCorresponds to variant dbSNP:rs12366671Ensembl. | 1 | |
Natural variantiVAR_061000 | 525 | E → K3 PublicationsCorresponds to variant dbSNP:rs1990312Ensembl. | 1 | |
Natural variantiVAR_055490 | 661 | I → T. Corresponds to variant dbSNP:rs1990313Ensembl. | 1 | |
Natural variantiVAR_055491 | 700 | S → F. Corresponds to variant dbSNP:rs2041291Ensembl. | 1 | |
Natural variantiVAR_059112 | 700 | S → P. Corresponds to variant dbSNP:rs2041290Ensembl. | 1 | |
Natural variantiVAR_055492 | 725 | S → L. Corresponds to variant dbSNP:rs2072357Ensembl. | 1 | |
Natural variantiVAR_036428 | 831 | R → C in a colorectal cancer sample; somatic mutation. 1 PublicationCorresponds to variant dbSNP:rs143517596Ensembl. | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U85715 mRNA Translation: AAD21218.1 AF087003 mRNA Translation: AAC35854.1 AF093408 mRNA Translation: AAC63371.1 AK292451 mRNA Translation: BAF85140.1 AC005832 Genomic DNA No translation available. BC047535 mRNA Translation: AAH47535.1 |
CCDSi | CCDS8531.1 |
RefSeqi | NP_001265238.1, NM_001278309.1 NP_006413.3, NM_006422.3 XP_005253721.1, XM_005253664.2 XP_011519210.1, XM_011520908.1 XP_011519211.1, XM_011520909.1 XP_011519212.1, XM_011520910.2 |
Genome annotation databases
Ensembli | ENST00000228850; ENSP00000228850; ENSG00000111254 ENST00000545990; ENSP00000440994; ENSG00000111254 |
GeneIDi | 10566 |
KEGGi | hsa:10566 |
Keywords - Coding sequence diversityi
PolymorphismSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U85715 mRNA Translation: AAD21218.1 AF087003 mRNA Translation: AAC35854.1 AF093408 mRNA Translation: AAC63371.1 AK292451 mRNA Translation: BAF85140.1 AC005832 Genomic DNA No translation available. BC047535 mRNA Translation: AAH47535.1 |
CCDSi | CCDS8531.1 |
RefSeqi | NP_001265238.1, NM_001278309.1 NP_006413.3, NM_006422.3 XP_005253721.1, XM_005253664.2 XP_011519210.1, XM_011520908.1 XP_011519211.1, XM_011520909.1 XP_011519212.1, XM_011520910.2 |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
BioGRIDi | 115817, 8 interactors |
IntActi | O75969, 5 interactors |
STRINGi | 9606.ENSP00000228850 |
PTM databases
iPTMneti | O75969 |
PhosphoSitePlusi | O75969 |
Polymorphism and mutation databases
BioMutai | AKAP3 |
Proteomic databases
EPDi | O75969 |
jPOSTi | O75969 |
MassIVEi | O75969 |
PaxDbi | O75969 |
PeptideAtlasi | O75969 |
PRIDEi | O75969 |
ProteomicsDBi | 50329 |
Protocols and materials databases
Antibodypediai | 22296, 173 antibodies |
Genome annotation databases
Ensembli | ENST00000228850; ENSP00000228850; ENSG00000111254 ENST00000545990; ENSP00000440994; ENSG00000111254 |
GeneIDi | 10566 |
KEGGi | hsa:10566 |
Organism-specific databases
CTDi | 10566 |
DisGeNETi | 10566 |
EuPathDBi | HostDB:ENSG00000111254.7 |
GeneCardsi | AKAP3 |
HGNCi | HGNC:373, AKAP3 |
HPAi | ENSG00000111254, Tissue enriched (testis) |
MIMi | 604689, gene |
neXtProti | NX_O75969 |
OpenTargetsi | ENSG00000111254 |
PharmGKBi | PA24667 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | ENOG502SM7F, Eukaryota |
GeneTreei | ENSGT00940000153313 |
HOGENOMi | CLU_017072_0_0_1 |
InParanoidi | O75969 |
OMAi | DWKMDTS |
OrthoDBi | 221175at2759 |
PhylomeDBi | O75969 |
TreeFami | TF105403 |
Enzyme and pathway databases
PathwayCommonsi | O75969 |
Miscellaneous databases
BioGRID-ORCSi | 10566, 12 hits in 841 CRISPR screens |
ChiTaRSi | AKAP3, human |
GeneWikii | AKAP3 |
GenomeRNAii | 10566 |
Pharosi | O75969, Tbio |
PROi | PR:O75969 |
RNActi | O75969, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000111254, Expressed in testis and 123 other tissues |
ExpressionAtlasi | O75969, baseline and differential |
Genevisiblei | O75969, HS |
Family and domain databases
InterProi | View protein in InterPro IPR020799, AKAP_110 IPR018292, AKAP_110_C IPR018459, RII_binding_1 IPR008382, SPHK1-interactor_AKAP_110 |
PANTHERi | PTHR10226, PTHR10226, 1 hit |
Pfami | View protein in Pfam PF05716, AKAP_110, 1 hit PF10522, RII_binding_1, 1 hit |
SMARTi | View protein in SMART SM00807, AKAP_110, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | AKAP3_HUMAN | |
Accessioni | O75969Primary (citable) accession number: O75969 Secondary accession number(s): O75945, Q86X01, Q9UM61 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | June 1, 2001 |
Last sequence update: | December 15, 2009 | |
Last modified: | December 2, 2020 | |
This is version 158 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
Direct protein sequencing, Reference proteomeDocuments
- Human polymorphisms and disease mutations
Index of human polymorphisms and disease mutations - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families - Human chromosome 12
Human chromosome 12: entries, gene names and cross-references to MIM - Human entries with polymorphisms or disease mutations
List of human entries with polymorphisms or disease mutations