UniProtKB - O75962 (TRIO_HUMAN)
Triple functional domain protein
TRIO
Functioni
Guanine nucleotide exchange factor (GEF) for RHOA and RAC1 GTPases (PubMed:8643598, PubMed:22155786, PubMed:27418539).
Involved in coordinating actin remodeling, which is necessary for cell migration and growth (PubMed:10341202, PubMed:22155786).
Plays a key role in the regulation of neurite outgrowth and lamellipodia formation (PubMed:32109419).
In developing hippocampal neurons, limits dendrite formation, without affecting the establishment of axon polarity. Once dendrites are formed, involved in the control of synaptic function by regulating the endocytosis of AMPA-selective glutamate receptors (AMPARs) at CA1 excitatory synapses (By similarity).
May act as a regulator of adipogenesis (By similarity).
By similarity5 PublicationsCatalytic activityi
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 2825 | ATPPROSITE-ProRule annotation | 1 | |
Active sitei | 2915 | By similarity | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 2802 – 2810 | ATPPROSITE-ProRule annotation | 9 |
GO - Molecular functioni
- ATP binding Source: UniProtKB-KW
- guanyl-nucleotide exchange factor activity Source: GO_Central
- protein serine/threonine/tyrosine kinase activity Source: RHEA
- protein serine/threonine kinase activity Source: ProtInc
- protein serine kinase activity Source: RHEA
GO - Biological processi
- axon guidance Source: GO_Central
- central nervous system development Source: InterPro
- negative regulation of fat cell differentiation Source: UniProtKB
- neuron projection morphogenesis Source: UniProtKB
- regulation of small GTPase mediated signal transduction Source: Reactome
- transmembrane receptor protein tyrosine phosphatase signaling pathway Source: ProtInc
Keywordsi
Molecular function | Guanine-nucleotide releasing factor, Kinase, Serine/threonine-protein kinase, Transferase |
Ligand | ATP-binding, Nucleotide-binding |
Enzyme and pathway databases
PathwayCommonsi | O75962 |
Reactomei | R-HSA-193648, NRAGE signals death through JNK R-HSA-416476, G alpha (q) signalling events R-HSA-416482, G alpha (12/13) signalling events R-HSA-418885, DCC mediated attractive signaling R-HSA-8980692, RHOA GTPase cycle R-HSA-9013148, CDC42 GTPase cycle R-HSA-9013149, RAC1 GTPase cycle R-HSA-9013404, RAC2 GTPase cycle R-HSA-9013408, RHOG GTPase cycle R-HSA-9013409, RHOJ GTPase cycle R-HSA-9013423, RAC3 GTPase cycle |
SignaLinki | O75962 |
SIGNORi | O75962 |
Names & Taxonomyi
Protein namesi | Recommended name: Triple functional domain protein (EC:2.7.11.1)Alternative name(s): PTPRF-interacting protein |
Gene namesi | Name:TRIO |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:12303, TRIO |
MIMi | 601893, gene |
neXtProti | NX_O75962 |
VEuPathDBi | HostDB:ENSG00000038382 |
Subcellular locationi
Cytoplasm and Cytosol
- Cytoplasm 1 Publication
Other locations
- Cell projection By similarity
Cytosol
- cytosol Source: Reactome
Other locations
- cell projection Source: UniProtKB-SubCell
- cytoplasm Source: GO_Central
- extrinsic component of membrane Source: GO_Central
Keywords - Cellular componenti
Cell projection, CytoplasmPathology & Biotechi
Involvement in diseasei
Intellectual developmental disorder, autosomal dominant 44, with microcephaly (MRD44)3 Publications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_083915 | 768 – 3097 | Missing in MRD44. 1 PublicationAdd BLAST | 2330 | |
Natural variantiVAR_077093 | 924 | R → S in MRD44; unknown pathological significance. 1 PublicationCorresponds to variant dbSNP:rs772072746Ensembl. | 1 | |
Natural variantiVAR_077095 | 1238 | Y → H in MRD44; unknown pathological significance. 1 PublicationCorresponds to variant dbSNP:rs756004023Ensembl. | 1 | |
Natural variantiVAR_083920 | 1299 | E → K in MRD44; severely decreased activation of RAC1-mediated signaling; severely decreased neurite outgrowth. 1 Publication | 1 | |
Natural variantiVAR_077096 | 1428 | R → Q in MRD44; severely decreased activation of RAC1-mediated signaling; severely decreased neurite outgrowth. 2 PublicationsCorresponds to variant dbSNP:rs879255626EnsemblClinVar. | 1 | |
Natural variantiVAR_077097 | 1461 | P → T in MRD44; no effect on RAC1-mediated signaling; no effect on neurite outgrowth. 2 PublicationsCorresponds to variant dbSNP:rs879255627EnsemblClinVar. | 1 | |
Natural variantiVAR_083922 | 1469 | H → R in MRD44; decreased activation of RAC1-mediated signaling; severely decreased neurite outgrowth. 1 PublicationCorresponds to variant dbSNP:rs1554070777EnsemblClinVar. | 1 | |
Natural variantiVAR_077098 | 1922 | A → T in MRD44; unknown pathological significance. 1 Publication | 1 | |
Natural variantiVAR_077099 | 1939 | S → N in MRD44; unknown pathological significance. 1 Publication | 1 | |
Natural variantiVAR_077100 | 2201 | L → V in MRD44; unknown pathological significance. 1 PublicationCorresponds to variant dbSNP:rs771342869Ensembl. | 1 | |
Natural variantiVAR_077101 | 2247 | E → D in MRD44; unknown pathological significance. 1 PublicationCorresponds to variant dbSNP:rs1258664728Ensembl. | 1 | |
Natural variantiVAR_077102 | 2707 | R → Q in MRD44; unknown pathological significance. 1 PublicationCorresponds to variant dbSNP:rs768858988EnsemblClinVar. | 1 |
Intellectual developmental disorder, autosomal dominant 63, with macrocephaly (MRD63)2 Publications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_083916 | 1075 | T → I in MRD63; slightly increased occipitofrontal circumference; increased activation of RAC1-mediated signaling; increased lamellipodia formation. 1 Publication | 1 | |
Natural variantiVAR_083917 | 1078 | R → G in MRD63; increased activation of RAC1-mediated signaling; increased neurite outgrowth. 1 Publication | 1 | |
Natural variantiVAR_083918 | 1078 | R → Q in MRD63; increased activation of RAC1-mediated signaling; increased neurite outgrowth. 1 Publication | 1 | |
Natural variantiVAR_083919 | 1078 | R → W in MRD63; increased activation of RAC1-mediated signaling; increased neurite outgrowth. 1 PublicationCorresponds to variant dbSNP:rs1554065887Ensembl. | 1 | |
Natural variantiVAR_077094 | 1080 | N → I in MRD63; slightly increased occipitofrontal circumference; increased activation of RAC1-mediated signaling; increased neurite outgrowth. 2 PublicationsCorresponds to variant dbSNP:rs879255628EnsemblClinVar. | 1 | |
Natural variantiVAR_083921 | 1461 | P → L in MRD63; slightly increased occipitofrontal circumference; slightly increased RAC1-mediated signaling; slightly increased neurite outgrowth. 1 Publication | 1 |
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 1299 | E → A: 50% decrease in nucleotide exchange activity. 1 Publication | 1 | |
Mutagenesisi | 1303 | T → A: 40% decrease in nucleotide exchange activity. 1 Publication | 1 | |
Mutagenesisi | 1389 | N → A: No change in nucleotide exchange activity. 1 Publication | 1 | |
Mutagenesisi | 1426 | V → A: 90% decrease in nucleotide exchange activity. 1 Publication | 1 | |
Mutagenesisi | 1427 | Q → A: 80% decrease in nucleotide exchange activity. 1 Publication | 1 | |
Mutagenesisi | 1428 | R → A: 80% decrease in nucleotide exchange activity. 1 Publication | 1 | |
Mutagenesisi | 1430 | T → A: 80% decrease in nucleotide exchange activity. 1 Publication | 1 | |
Mutagenesisi | 1431 | K → A: Loss of nucleotide exchange activity. 1 Publication | 1 | |
Mutagenesisi | 1434 | L → A: 40% decrease in nucleotide exchange activity. 1 Publication | 1 | |
Mutagenesisi | 1437 | K → A: No change in nucleotide exchange activity. 1 Publication | 1 | |
Mutagenesisi | 1438 | E → A: 30% decrease in nucleotide exchange activity. 1 Publication | 1 | |
Mutagenesisi | 2917 | K → A: Expected to disrupt kinase activity. Causes reorganization of the actin cytoskeleton in the absence of NGF. 1 Publication | 1 |
Keywords - Diseasei
Disease variant, Mental retardationOrganism-specific databases
DisGeNETi | 7204 |
GeneReviewsi | TRIO |
MalaCardsi | TRIO |
MIMi | 617061, phenotype 618825, phenotype |
OpenTargetsi | ENSG00000038382 |
Orphaneti | 476126, Micrognathia-recurrent infections-behavioral abnormalities-mild intellectual disability syndrome |
PharmGKBi | PA36982 |
Miscellaneous databases
Pharosi | O75962, Tbio |
Chemistry databases
ChEMBLi | CHEMBL4523153 |
Genetic variation databases
BioMutai | TRIO |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000080978 | 1 – 3097 | Triple functional domain proteinAdd BLAST | 3097 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 1627 | PhosphoserineBy similarity | 1 | |
Modified residuei | 1632 | PhosphoserineBy similarity | 1 | |
Modified residuei | 1633 | PhosphoserineBy similarity | 1 | |
Modified residuei | 1824 | PhosphothreonineBy similarity | 1 | |
Modified residuei | 2282 | PhosphoserineCombined sources | 1 | |
Modified residuei | 2455 | PhosphoserineCombined sources | 1 | |
Modified residuei | 2459 | PhosphoserineCombined sources | 1 | |
Modified residuei | 2631 | PhosphoserineCombined sources | 1 | |
Disulfide bondi | 2696 ↔ 2759 | PROSITE-ProRule annotation |
Post-translational modificationi
Keywords - PTMi
Disulfide bond, PhosphoproteinProteomic databases
EPDi | O75962 |
jPOSTi | O75962 |
MassIVEi | O75962 |
MaxQBi | O75962 |
PaxDbi | O75962 |
PeptideAtlasi | O75962 |
PRIDEi | O75962 |
ProteomicsDBi | 50323 [O75962-1] 50324 [O75962-2] 50325 [O75962-3] 50326 [O75962-4] 50327 [O75962-5] |
PTM databases
iPTMneti | O75962 |
PhosphoSitePlusi | O75962 |
Expressioni
Tissue specificityi
Gene expression databases
Bgeei | ENSG00000038382, Expressed in stomach and 233 other tissues |
ExpressionAtlasi | O75962, baseline and differential |
Genevisiblei | O75962, HS |
Organism-specific databases
HPAi | ENSG00000038382, Low tissue specificity |
Interactioni
Subunit structurei
Interacts with CARMIL1 (PubMed:19846667).
Interacts with PTPRF/LAR (PubMed:8643598).
Interacts with ANKRD26 (PubMed:22666460).
Interacts with Bassoon/BSN and Piccolo/PCLO (By similarity).
Interacts with the cytoplasmic region of the heterodimer formed by NGFR and SORCS2. ProNGF binding mediates dissociation of TRIO from the receptor complex (PubMed:22155786).
By similarity4 PublicationsBinary interactionsi
O75962
With | #Exp. | IntAct |
---|---|---|
DISC1 [Q9NRI5] | 3 | EBI-718519,EBI-529989 |
Isoform 4 [O75962-4]
With | #Exp. | IntAct |
---|---|---|
RAC1 - isoform A [P63000-1] | 2 | EBI-15915736,EBI-7212896 |
Disc1 - isoform 1 [Q811T9-1] from Mus musculus. | 2 | EBI-15915736,EBI-15881527 |
Protein-protein interaction databases
BioGRIDi | 113055, 73 interactors |
DIPi | DIP-37578N |
IntActi | O75962, 35 interactors |
MINTi | O75962 |
STRINGi | 9606.ENSP00000339299 |
Miscellaneous databases
RNActi | O75962, protein |
Structurei
Secondary structure
3D structure databases
SMRi | O75962 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | O75962 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 65 – 210 | CRAL-TRIOPROSITE-ProRule annotationAdd BLAST | 146 | |
Repeati | 311 – 418 | Spectrin 1Add BLAST | 108 | |
Repeati | 538 – 644 | Spectrin 2Add BLAST | 107 | |
Repeati | 878 – 984 | Spectrin 3Add BLAST | 107 | |
Repeati | 1109 – 1216 | Spectrin 4Add BLAST | 108 | |
Domaini | 1292 – 1467 | DH 1PROSITE-ProRule annotationAdd BLAST | 176 | |
Domaini | 1480 – 1591 | PH 1PROSITE-ProRule annotationAdd BLAST | 112 | |
Domaini | 1656 – 1721 | SH3 1PROSITE-ProRule annotationAdd BLAST | 66 | |
Domaini | 1969 – 2145 | DH 2PROSITE-ProRule annotationAdd BLAST | 177 | |
Domaini | 2157 – 2271 | PH 2PROSITE-ProRule annotationAdd BLAST | 115 | |
Domaini | 2551 – 2616 | SH3 2PROSITE-ProRule annotationAdd BLAST | 66 | |
Domaini | 2685 – 2775 | Ig-like C2-typeAdd BLAST | 91 | |
Domaini | 2796 – 3052 | Protein kinasePROSITE-ProRule annotationAdd BLAST | 257 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1601 – 1650 | DisorderedSequence analysisAdd BLAST | 50 | |
Regioni | 1779 – 1906 | DisorderedSequence analysisAdd BLAST | 128 | |
Regioni | 1927 – 1965 | DisorderedSequence analysisAdd BLAST | 39 | |
Regioni | 2287 – 2552 | DisorderedSequence analysisAdd BLAST | 266 | |
Regioni | 2639 – 2660 | DisorderedSequence analysisAdd BLAST | 22 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 1628 – 1650 | Polar residuesSequence analysisAdd BLAST | 23 | |
Compositional biasi | 1806 – 1835 | Basic and acidic residuesSequence analysisAdd BLAST | 30 | |
Compositional biasi | 1836 – 1855 | Polar residuesSequence analysisAdd BLAST | 20 | |
Compositional biasi | 1878 – 1906 | Polar residuesSequence analysisAdd BLAST | 29 | |
Compositional biasi | 1931 – 1955 | Polar residuesSequence analysisAdd BLAST | 25 | |
Compositional biasi | 2319 – 2340 | Polar residuesSequence analysisAdd BLAST | 22 | |
Compositional biasi | 2353 – 2371 | Polar residuesSequence analysisAdd BLAST | 19 | |
Compositional biasi | 2372 – 2390 | Pro residuesSequence analysisAdd BLAST | 19 | |
Compositional biasi | 2397 – 2414 | Basic and acidic residuesSequence analysisAdd BLAST | 18 | |
Compositional biasi | 2480 – 2513 | Polar residuesSequence analysisAdd BLAST | 34 | |
Compositional biasi | 2524 – 2552 | Polar residuesSequence analysisAdd BLAST | 29 |
Domaini
Sequence similaritiesi
Keywords - Domaini
Immunoglobulin domain, Repeat, SH3 domainPhylogenomic databases
eggNOGi | KOG0032, Eukaryota KOG4240, Eukaryota |
GeneTreei | ENSGT00940000154766 |
HOGENOMi | CLU_000288_76_3_1 |
InParanoidi | O75962 |
OMAi | HTHVKEX |
OrthoDBi | 5761at2759 |
PhylomeDBi | O75962 |
TreeFami | TF318080 |
Family and domain databases
CDDi | cd00160, RhoGEF, 2 hits cd00170, SEC14, 1 hit cd00176, SPEC, 6 hits |
Gene3Di | 1.20.900.10, 2 hits 2.30.29.30, 2 hits 2.60.40.10, 1 hit 3.40.525.10, 1 hit |
InterProi | View protein in InterPro IPR001251, CRAL-TRIO_dom IPR036865, CRAL-TRIO_dom_sf IPR035899, DBL_dom_sf IPR000219, DH-domain IPR007110, Ig-like_dom IPR036179, Ig-like_dom_sf IPR013783, Ig-like_fold IPR013098, Ig_I-set IPR003599, Ig_sub IPR003598, Ig_sub2 IPR028570, Kalirin/TRIO IPR011009, Kinase-like_dom_sf IPR011993, PH-like_dom_sf IPR001849, PH_domain IPR000719, Prot_kinase_dom IPR008271, Ser/Thr_kinase_AS IPR036028, SH3-like_dom_sf IPR001452, SH3_domain IPR018159, Spectrin/alpha-actinin IPR002017, Spectrin_repeat |
PANTHERi | PTHR22826:SF104, PTHR22826:SF104, 1 hit |
Pfami | View protein in Pfam PF00650, CRAL_TRIO, 1 hit PF07679, I-set, 1 hit PF00169, PH, 2 hits PF00069, Pkinase, 1 hit PF00621, RhoGEF, 2 hits PF00018, SH3_1, 1 hit PF00435, Spectrin, 4 hits |
SMARTi | View protein in SMART SM00409, IG, 1 hit SM00408, IGc2, 1 hit SM00233, PH, 2 hits SM00325, RhoGEF, 2 hits SM00220, S_TKc, 1 hit SM00516, SEC14, 1 hit SM00326, SH3, 2 hits SM00150, SPEC, 6 hits |
SUPFAMi | SSF48065, SSF48065, 2 hits SSF48726, SSF48726, 1 hit SSF50044, SSF50044, 2 hits SSF52087, SSF52087, 1 hit SSF56112, SSF56112, 1 hit |
PROSITEi | View protein in PROSITE PS50191, CRAL_TRIO, 1 hit PS50010, DH_2, 2 hits PS50835, IG_LIKE, 1 hit PS50003, PH_DOMAIN, 2 hits PS50011, PROTEIN_KINASE_DOM, 1 hit PS00108, PROTEIN_KINASE_ST, 1 hit PS50002, SH3, 2 hits |
s (5+)i Sequence
Sequence statusi: Complete.
This entry describes 5 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 5 described isoforms and 4 potential isoforms that are computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MSGSSGGAAA PAASSGPAAA ASAAGSGCGG GAGEGAEEAA KDLADIAAFF
60 70 80 90 100
RSGFRKNDEM KAMDVLPILK EKVAYLSGGR DKRGGPILTF PARSNHDRIR
110 120 130 140 150
QEDLRRLISY LACIPSEEVC KRGFTVIVDM RGSKWDSIKP LLKILQESFP
160 170 180 190 200
CCIHVALIIK PDNFWQKQRT NFGSSKFEFE TNMVSLEGLT KVVDPSQLTP
210 220 230 240 250
EFDGCLEYNH EEWIEIRVAF EDYISNATHM LSRLEELQDI LAKKELPQDL
260 270 280 290 300
EGARNMIEEH SQLKKKVIKA PIEDLDLEGQ KLLQRIQSSE SFPKKNSGSG
310 320 330 340 350
NADLQNLLPK VSTMLDRLHS TRQHLHQMWH VRKLKLDQCF QLRLFEQDAE
360 370 380 390 400
KMFDWITHNK GLFLNSYTEI GTSHPHAMEL QTQHNHFAMN CMNVYVNINR
410 420 430 440 450
IMSVANRLVE SGHYASQQIR QIASQLEQEW KAFAAALDER STLLDMSSIF
460 470 480 490 500
HQKAEKYMSN VDSWCKACGE VDLPSELQDL EDAIHHHQGI YEHITLAYSE
510 520 530 540 550
VSQDGKSLLD KLQRPLTPGS SDSLTASANY SKAVHHVLDV IHEVLHHQRQ
560 570 580 590 600
LENIWQHRKV RLHQRLQLCV FQQDVQQVLD WIENHGEAFL SKHTGVGKSL
610 620 630 640 650
HRARALQKRH EDFEEVAQNT YTNADKLLEA AEQLAQTGEC DPEEIYQAAH
660 670 680 690 700
QLEDRIQDFV RRVEQRKILL DMSVSFHTHV KELWTWLEEL QKELLDDVYA
710 720 730 740 750
ESVEAVQDLI KRFGQQQQTT LQVTVNVIKE GEDLIQQLRD SAISSNKTPH
760 770 780 790 800
NSSINHIETV LQQLDEAQSQ MEELFQERKI KLELFLQLRI FERDAIDIIS
810 820 830 840 850
DLESWNDELS QQMNDFDTED LTIAEQRLQH HADKALTMNN LTFDVIHQGQ
860 870 880 890 900
DLLQYVNEVQ ASGVELLCDR DVDMATRVQD LLEFLHEKQQ ELDLAAEQHR
910 920 930 940 950
KHLEQCVQLR HLQAEVKQVL GWIRNGESML NAGLITASSL QEAEQLQREH
960 970 980 990 1000
EQFQHAIEKT HQSALQVQQK AEAMLQANHY DMDMIRDCAE KVASHWQQLM
1010 1020 1030 1040 1050
LKMEDRLKLV NASVAFYKTS EQVCSVLESL EQEYKREEDW CGGADKLGPN
1060 1070 1080 1090 1100
SETDHVTPMI SKHLEQKEAF LKACTLARRN ADVFLKYLHR NSVNMPGMVT
1110 1120 1130 1140 1150
HIKAPEQQVK NILNELFQRE NRVLHYWTMR KRRLDQCQQY VVFERSAKQA
1160 1170 1180 1190 1200
LEWIHDNGEF YLSTHTSTGS SIQHTQELLK EHEEFQITAK QTKERVKLLI
1210 1220 1230 1240 1250
QLADGFCEKG HAHAAEIKKC VTAVDKRYRD FSLRMEKYRT SLEKALGISS
1260 1270 1280 1290 1300
DSNKSSKSLQ LDIIPASIPG SEVKLRDAAH ELNEEKRKSA RRKEFIMAEL
1310 1320 1330 1340 1350
IQTEKAYVRD LRECMDTYLW EMTSGVEEIP PGIVNKELII FGNMQEIYEF
1360 1370 1380 1390 1400
HNNIFLKELE KYEQLPEDVG HCFVTWADKF QMYVTYCKNK PDSTQLILEH
1410 1420 1430 1440 1450
AGSYFDEIQQ RHGLANSISS YLIKPVQRIT KYQLLLKELL TCCEEGKGEI
1460 1470 1480 1490 1500
KDGLEVMLSV PKRANDAMHL SMLEGFDENI ESQGELILQE SFQVWDPKTL
1510 1520 1530 1540 1550
IRKGRERHLF LFEMSLVFSK EVKDSSGRSK YLYKSKLFTS ELGVTEHVEG
1560 1570 1580 1590 1600
DPCKFALWVG RTPTSDNKIV LKASSIENKQ DWIKHIREVI QERTIHLKGA
1610 1620 1630 1640 1650
LKEPIHIPKT APATRQKGRR DGEDLDSQGD GSSQPDTISI ASRTSQNTLD
1660 1670 1680 1690 1700
SDKLSGGCEL TVVIHDFTAC NSNELTIRRG QTVEVLERPH DKPDWCLVRT
1710 1720 1730 1740 1750
TDRSPAAEGL VPCGSLCIAH SRSSMEMEGI FNHKDSLSVS SNDASPPASV
1760 1770 1780 1790 1800
ASLQPHMIGA QSSPGPKRPG NTLRKWLTSP VRRLSSGKAD GHVKKLAHKH
1810 1820 1830 1840 1850
KKSREVRKSA DAGSQKDSDD SAATPQDETV EERGRNEGLS SGTLSKSSSS
1860 1870 1880 1890 1900
GMQSCGEEEG EEGADAVPLP PPMAIQQHSL LQPDSQDDKA SSRLLVRPTS
1910 1920 1930 1940 1950
SETPSAAELV SAIEELVKSK MALEDRPSSL LVDQGDSSSP SFNPSDNSLL
1960 1970 1980 1990 2000
SSSSPIDEME ERKSSSLKRR HYVLQELVET ERDYVRDLGY VVEGYMALMK
2010 2020 2030 2040 2050
EDGVPDDMKG KDKIVFGNIH QIYDWHRDFF LGELEKCLED PEKLGSLFVK
2060 2070 2080 2090 2100
HERRLHMYIA YCQNKPKSEH IVSEYIDTFF EDLKQRLGHR LQLTDLLIKP
2110 2120 2130 2140 2150
VQRIMKYQLL LKDFLKYSKK ASLDTSELER AVEVMCIVPR RCNDMMNVGR
2160 2170 2180 2190 2200
LQGFDGKIVA QGKLLLQDTF LVTDQDAGLL PRCRERRIFL FEQIVIFSEP
2210 2220 2230 2240 2250
LDKKKGFSMP GFLFKNSIKV SCLCLEENVE NDPCKFALTS RTGDVVETFI
2260 2270 2280 2290 2300
LHSSSPSVRQ TWIHEINQIL ENQRNFLNAL TSPIEYQRNH SGGGGGGGSG
2310 2320 2330 2340 2350
GSGGGGGSGG GGAPSGGSGH SGGPSSCGGA PSTSRSRPSR IPQPVRHHPP
2360 2370 2380 2390 2400
VLVSSAASSQ AEADKMSGTS TPGPSLPPPG AAPEAGPSAP SRRPPGADAE
2410 2420 2430 2440 2450
GSEREAEPIP KMKVLESPRK GAANASGSSP DAPAKDARAS LGTLPLGKPR
2460 2470 2480 2490 2500
AGAASPLNSP LSSAVPSLGK EPFPPSSPLQ KGGSFWSSIP ASPASRPGSF
2510 2520 2530 2540 2550
TFPGDSDSLQ RQTPRHAAPG KDTDRMSTCS SASEQSVQST QSNGSESSSS
2560 2570 2580 2590 2600
SNISTMLVTH DYTAVKEDEI NVYQGEVVQI LASNQQNMFL VFRAATDQCP
2610 2620 2630 2640 2650
AAEGWIPGFV LGHTSAVIVE NPDGTLKKST SWHTALRLRK KSEKKDKDGK
2660 2670 2680 2690 2700
REGKLENGYR KSREGLSNKV SVKLLNPNYI YDVPPEFVIP LSEVTCETGE
2710 2720 2730 2740 2750
TVVLRCRVCG RPKASITWKG PEHNTLNNDG HYSISYSDLG EATLKIVGVT
2760 2770 2780 2790 2800
TEDDGIYTCI AVNDMGSASS SASLRVLGPG MDGIMVTWKD NFDSFYSEVA
2810 2820 2830 2840 2850
ELGRGRFSVV KKCDQKGTKR AVATKFVNKK LMKRDQVTHE LGILQSLQHP
2860 2870 2880 2890 2900
LLVGLLDTFE TPTSYILVLE MADQGRLLDC VVRWGSLTEG KIRAHLGEVL
2910 2920 2930 2940 2950
EAVRYLHNCR IAHLDLKPEN ILVDESLAKP TIKLADFGDA VQLNTTYYIH
2960 2970 2980 2990 3000
QLLGNPEFAA PEIILGNPVS LTSDTWSVGV LTYVLLSGVS PFLDDSVEET
3010 3020 3030 3040 3050
CLNICRLDFS FPDDYFKGVS QKAKEFVCFL LQEDPAKRPS AALALQEQWL
3060 3070 3080 3090
QAGNGRSTGV LDTSRLTSFI ERRKHQNDVR PIRSIKNFLQ SRLLPRV
Computationally mapped potential isoform sequencesi
There are 4 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketE7EPJ7 | E7EPJ7_HUMAN | Non-specific serine/threonine prote... | TRIO | 2,546 | Annotation score: | ||
E7EWP2 | E7EWP2_HUMAN | Triple functional domain protein | TRIO | 2,309 | Annotation score: | ||
A0A1W2PRD7 | A0A1W2PRD7_HUMAN | Triple functional domain protein | TRIO | 458 | Annotation score: | ||
F5H228 | F5H228_HUMAN | Triple functional domain protein | TRIO | 1,508 | Annotation score: |
Sequence cautioni
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 550 – 553 | QLEN → HVRT in AAC34245 (PubMed:8643598).Curated | 4 | |
Sequence conflicti | 550 – 553 | QLEN → HVRT in AAC43042 (Ref. 6) Curated | 4 | |
Sequence conflicti | 574 | D → E in AAC34245 (PubMed:8643598).Curated | 1 | |
Sequence conflicti | 574 | D → E in AAC43042 (Ref. 6) Curated | 1 | |
Sequence conflicti | 787 – 788 | QL → HV in AAC34245 (PubMed:8643598).Curated | 2 | |
Sequence conflicti | 787 – 788 | QL → HV in AAC43042 (Ref. 6) Curated | 2 |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_041899 | 291 | S → T1 PublicationCorresponds to variant dbSNP:rs55772118Ensembl. | 1 | |
Natural variantiVAR_059802 | 348 | D → E. Corresponds to variant dbSNP:rs16903367Ensembl. | 1 | |
Natural variantiVAR_083915 | 768 – 3097 | Missing in MRD44. 1 PublicationAdd BLAST | 2330 | |
Natural variantiVAR_077093 | 924 | R → S in MRD44; unknown pathological significance. 1 PublicationCorresponds to variant dbSNP:rs772072746Ensembl. | 1 | |
Natural variantiVAR_083916 | 1075 | T → I in MRD63; slightly increased occipitofrontal circumference; increased activation of RAC1-mediated signaling; increased lamellipodia formation. 1 Publication | 1 | |
Natural variantiVAR_083917 | 1078 | R → G in MRD63; increased activation of RAC1-mediated signaling; increased neurite outgrowth. 1 Publication | 1 | |
Natural variantiVAR_083918 | 1078 | R → Q in MRD63; increased activation of RAC1-mediated signaling; increased neurite outgrowth. 1 Publication | 1 | |
Natural variantiVAR_083919 | 1078 | R → W in MRD63; increased activation of RAC1-mediated signaling; increased neurite outgrowth. 1 PublicationCorresponds to variant dbSNP:rs1554065887Ensembl. | 1 | |
Natural variantiVAR_077094 | 1080 | N → I in MRD63; slightly increased occipitofrontal circumference; increased activation of RAC1-mediated signaling; increased neurite outgrowth. 2 PublicationsCorresponds to variant dbSNP:rs879255628EnsemblClinVar. | 1 | |
Natural variantiVAR_077095 | 1238 | Y → H in MRD44; unknown pathological significance. 1 PublicationCorresponds to variant dbSNP:rs756004023Ensembl. | 1 | |
Natural variantiVAR_083920 | 1299 | E → K in MRD44; severely decreased activation of RAC1-mediated signaling; severely decreased neurite outgrowth. 1 Publication | 1 | |
Natural variantiVAR_069371 | 1368 | D → V Found in patient with severe intellectual disability; unknown pathological significance. 1 Publication | 1 | |
Natural variantiVAR_077096 | 1428 | R → Q in MRD44; severely decreased activation of RAC1-mediated signaling; severely decreased neurite outgrowth. 2 PublicationsCorresponds to variant dbSNP:rs879255626EnsemblClinVar. | 1 | |
Natural variantiVAR_083921 | 1461 | P → L in MRD63; slightly increased occipitofrontal circumference; slightly increased RAC1-mediated signaling; slightly increased neurite outgrowth. 1 Publication | 1 | |
Natural variantiVAR_077097 | 1461 | P → T in MRD44; no effect on RAC1-mediated signaling; no effect on neurite outgrowth. 2 PublicationsCorresponds to variant dbSNP:rs879255627EnsemblClinVar. | 1 | |
Natural variantiVAR_083922 | 1469 | H → R in MRD44; decreased activation of RAC1-mediated signaling; severely decreased neurite outgrowth. 1 PublicationCorresponds to variant dbSNP:rs1554070777EnsemblClinVar. | 1 | |
Natural variantiVAR_059803 | 1613 | A → T. Corresponds to variant dbSNP:rs16903474EnsemblClinVar. | 1 | |
Natural variantiVAR_041900 | 1644 | T → M1 PublicationCorresponds to variant dbSNP:rs55687522Ensembl. | 1 | |
Natural variantiVAR_041901 | 1690 | H → R1 PublicationCorresponds to variant dbSNP:rs56292586Ensembl. | 1 | |
Natural variantiVAR_077098 | 1922 | A → T in MRD44; unknown pathological significance. 1 Publication | 1 | |
Natural variantiVAR_077099 | 1939 | S → N in MRD44; unknown pathological significance. 1 Publication | 1 | |
Natural variantiVAR_041902 | 1978 | V → M in a metastatic melanoma sample; somatic mutation. 1 Publication | 1 | |
Natural variantiVAR_077100 | 2201 | L → V in MRD44; unknown pathological significance. 1 PublicationCorresponds to variant dbSNP:rs771342869Ensembl. | 1 | |
Natural variantiVAR_041903 | 2242 | T → M1 PublicationCorresponds to variant dbSNP:rs55916212EnsemblClinVar. | 1 | |
Natural variantiVAR_077101 | 2247 | E → D in MRD44; unknown pathological significance. 1 PublicationCorresponds to variant dbSNP:rs1258664728Ensembl. | 1 | |
Natural variantiVAR_069372 | 2563 | T → M Found in patient with severe intellectual disability; unknown pathological significance. 1 PublicationCorresponds to variant dbSNP:rs751663099EnsemblClinVar. | 1 | |
Natural variantiVAR_077102 | 2707 | R → Q in MRD44; unknown pathological significance. 1 PublicationCorresponds to variant dbSNP:rs768858988EnsemblClinVar. | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_023306 | 1 – 2501 | Missing in isoform 3. 1 PublicationAdd BLAST | 2501 | |
Alternative sequenceiVSP_037860 | 1 – 59 | Missing in isoform 4. 1 PublicationAdd BLAST | 59 | |
Alternative sequenceiVSP_004467 | 2368 | G → E in isoform 2. 1 Publication | 1 | |
Alternative sequenceiVSP_004468 | 2369 – 2544 | Missing in isoform 2. 1 PublicationAdd BLAST | 176 | |
Alternative sequenceiVSP_023307 | 2502 – 2544 | FPGDS…TQSNG → MLPSQAQGLLWWVFPLFPAS SLSYPPVSYRADGLARNTFL KAC in isoform 3. 1 PublicationAdd BLAST | 43 | |
Alternative sequenceiVSP_037861 | 2545 – 2563 | SESSS…THDYT → VSASGGPRPPAPLPLSRQL in isoform 5. 1 PublicationAdd BLAST | 19 | |
Alternative sequenceiVSP_037862 | 2564 – 3097 | Missing in isoform 5. 1 PublicationAdd BLAST | 534 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK131423 mRNA Translation: BAD18570.1 AC010419 Genomic DNA No translation available. AC016549 Genomic DNA No translation available. AC016654 Genomic DNA No translation available. AC016656 Genomic DNA No translation available. AC026456 Genomic DNA No translation available. CH471102 Genomic DNA Translation: EAX08047.1 CH471102 Genomic DNA Translation: EAX08048.1 BC035585 mRNA Translation: AAH35585.1 BC017268 mRNA Translation: AAH17268.1 U42390 mRNA Translation: AAC34245.1 Sequence problems. AF091395 mRNA Translation: AAC43042.1 Different initiation. AB209754 mRNA Translation: BAD92991.1 |
CCDSi | CCDS3883.1 [O75962-1] |
RefSeqi | NP_009049.2, NM_007118.3 [O75962-1] XP_011512412.1, XM_011514110.2 [O75962-4] |
Genome annotation databases
Ensembli | ENST00000344135.5; ENSP00000339291.5; ENSG00000038382.21 [O75962-3] ENST00000344204.9; ENSP00000339299.4; ENSG00000038382.21 |
GeneIDi | 7204 |
KEGGi | hsa:7204 |
MANE-Selecti | ENST00000344204.9; ENSP00000339299.4; NM_007118.4; NP_009049.2 |
UCSCi | uc003jff.4, human [O75962-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Web resourcesi
Atlas of Genetics and Cytogenetics in Oncology and Haematology |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK131423 mRNA Translation: BAD18570.1 AC010419 Genomic DNA No translation available. AC016549 Genomic DNA No translation available. AC016654 Genomic DNA No translation available. AC016656 Genomic DNA No translation available. AC026456 Genomic DNA No translation available. CH471102 Genomic DNA Translation: EAX08047.1 CH471102 Genomic DNA Translation: EAX08048.1 BC035585 mRNA Translation: AAH35585.1 BC017268 mRNA Translation: AAH17268.1 U42390 mRNA Translation: AAC34245.1 Sequence problems. AF091395 mRNA Translation: AAC43042.1 Different initiation. AB209754 mRNA Translation: BAD92991.1 |
CCDSi | CCDS3883.1 [O75962-1] |
RefSeqi | NP_009049.2, NM_007118.3 [O75962-1] XP_011512412.1, XM_011514110.2 [O75962-4] |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1NTY | X-ray | 1.70 | A | 1284-1594 | [»] | |
2NZ8 | X-ray | 2.00 | B | 1285-1594 | [»] | |
6D8Z | X-ray | 2.65 | A/B/C | 1960-2275 | [»] | |
SMRi | O75962 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 113055, 73 interactors |
DIPi | DIP-37578N |
IntActi | O75962, 35 interactors |
MINTi | O75962 |
STRINGi | 9606.ENSP00000339299 |
Chemistry databases
ChEMBLi | CHEMBL4523153 |
PTM databases
iPTMneti | O75962 |
PhosphoSitePlusi | O75962 |
Genetic variation databases
BioMutai | TRIO |
Proteomic databases
EPDi | O75962 |
jPOSTi | O75962 |
MassIVEi | O75962 |
MaxQBi | O75962 |
PaxDbi | O75962 |
PeptideAtlasi | O75962 |
PRIDEi | O75962 |
ProteomicsDBi | 50323 [O75962-1] 50324 [O75962-2] 50325 [O75962-3] 50326 [O75962-4] 50327 [O75962-5] |
Protocols and materials databases
ABCDi | O75962, 4 sequenced antibodies |
Antibodypediai | 2116, 79 antibodies from 20 providers |
DNASUi | 7204 |
Genome annotation databases
Ensembli | ENST00000344135.5; ENSP00000339291.5; ENSG00000038382.21 [O75962-3] ENST00000344204.9; ENSP00000339299.4; ENSG00000038382.21 |
GeneIDi | 7204 |
KEGGi | hsa:7204 |
MANE-Selecti | ENST00000344204.9; ENSP00000339299.4; NM_007118.4; NP_009049.2 |
UCSCi | uc003jff.4, human [O75962-1] |
Organism-specific databases
CTDi | 7204 |
DisGeNETi | 7204 |
GeneCardsi | TRIO |
GeneReviewsi | TRIO |
HGNCi | HGNC:12303, TRIO |
HPAi | ENSG00000038382, Low tissue specificity |
MalaCardsi | TRIO |
MIMi | 601893, gene 617061, phenotype 618825, phenotype |
neXtProti | NX_O75962 |
OpenTargetsi | ENSG00000038382 |
Orphaneti | 476126, Micrognathia-recurrent infections-behavioral abnormalities-mild intellectual disability syndrome |
PharmGKBi | PA36982 |
VEuPathDBi | HostDB:ENSG00000038382 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG0032, Eukaryota KOG4240, Eukaryota |
GeneTreei | ENSGT00940000154766 |
HOGENOMi | CLU_000288_76_3_1 |
InParanoidi | O75962 |
OMAi | HTHVKEX |
OrthoDBi | 5761at2759 |
PhylomeDBi | O75962 |
TreeFami | TF318080 |
Enzyme and pathway databases
PathwayCommonsi | O75962 |
Reactomei | R-HSA-193648, NRAGE signals death through JNK R-HSA-416476, G alpha (q) signalling events R-HSA-416482, G alpha (12/13) signalling events R-HSA-418885, DCC mediated attractive signaling R-HSA-8980692, RHOA GTPase cycle R-HSA-9013148, CDC42 GTPase cycle R-HSA-9013149, RAC1 GTPase cycle R-HSA-9013404, RAC2 GTPase cycle R-HSA-9013408, RHOG GTPase cycle R-HSA-9013409, RHOJ GTPase cycle R-HSA-9013423, RAC3 GTPase cycle |
SignaLinki | O75962 |
SIGNORi | O75962 |
Miscellaneous databases
BioGRID-ORCSi | 7204, 25 hits in 1105 CRISPR screens |
ChiTaRSi | TRIO, human |
EvolutionaryTracei | O75962 |
GeneWikii | TRIO_(gene) |
GenomeRNAii | 7204 |
Pharosi | O75962, Tbio |
PROi | PR:O75962 |
RNActi | O75962, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000038382, Expressed in stomach and 233 other tissues |
ExpressionAtlasi | O75962, baseline and differential |
Genevisiblei | O75962, HS |
Family and domain databases
CDDi | cd00160, RhoGEF, 2 hits cd00170, SEC14, 1 hit cd00176, SPEC, 6 hits |
Gene3Di | 1.20.900.10, 2 hits 2.30.29.30, 2 hits 2.60.40.10, 1 hit 3.40.525.10, 1 hit |
InterProi | View protein in InterPro IPR001251, CRAL-TRIO_dom IPR036865, CRAL-TRIO_dom_sf IPR035899, DBL_dom_sf IPR000219, DH-domain IPR007110, Ig-like_dom IPR036179, Ig-like_dom_sf IPR013783, Ig-like_fold IPR013098, Ig_I-set IPR003599, Ig_sub IPR003598, Ig_sub2 IPR028570, Kalirin/TRIO IPR011009, Kinase-like_dom_sf IPR011993, PH-like_dom_sf IPR001849, PH_domain IPR000719, Prot_kinase_dom IPR008271, Ser/Thr_kinase_AS IPR036028, SH3-like_dom_sf IPR001452, SH3_domain IPR018159, Spectrin/alpha-actinin IPR002017, Spectrin_repeat |
PANTHERi | PTHR22826:SF104, PTHR22826:SF104, 1 hit |
Pfami | View protein in Pfam PF00650, CRAL_TRIO, 1 hit PF07679, I-set, 1 hit PF00169, PH, 2 hits PF00069, Pkinase, 1 hit PF00621, RhoGEF, 2 hits PF00018, SH3_1, 1 hit PF00435, Spectrin, 4 hits |
SMARTi | View protein in SMART SM00409, IG, 1 hit SM00408, IGc2, 1 hit SM00233, PH, 2 hits SM00325, RhoGEF, 2 hits SM00220, S_TKc, 1 hit SM00516, SEC14, 1 hit SM00326, SH3, 2 hits SM00150, SPEC, 6 hits |
SUPFAMi | SSF48065, SSF48065, 2 hits SSF48726, SSF48726, 1 hit SSF50044, SSF50044, 2 hits SSF52087, SSF52087, 1 hit SSF56112, SSF56112, 1 hit |
PROSITEi | View protein in PROSITE PS50191, CRAL_TRIO, 1 hit PS50010, DH_2, 2 hits PS50835, IG_LIKE, 1 hit PS50003, PH_DOMAIN, 2 hits PS50011, PROTEIN_KINASE_DOM, 1 hit PS00108, PROTEIN_KINASE_ST, 1 hit PS50002, SH3, 2 hits |
MobiDBi | Search... |
Entry informationi
Entry namei | TRIO_HUMAN | |
Accessioni | O75962Primary (citable) accession number: O75962 Secondary accession number(s): D3DTD1 Q8IWK8 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | December 1, 2000 |
Last sequence update: | September 1, 2009 | |
Last modified: | May 25, 2022 | |
This is version 219 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Human and mouse protein kinases
Human and mouse protein kinases: classification and index - Human chromosome 5
Human chromosome 5: entries, gene names and cross-references to MIM - Human entries with genetic variants
List of human entries with genetic variants - Human variants curated from literature reports
Index of human variants curated from literature reports - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families