UniProtKB - O75934 (SPF27_HUMAN)
Pre-mRNA-splicing factor SPF27
BCAS2
Functioni
Required for pre-mRNA splicing as component of the activated spliceosome (PubMed:28502770, PubMed:28076346, PubMed:29360106, PubMed:29301961, PubMed:30705154).
Component of the PRP19-CDC5L complex that forms an integral part of the spliceosome and is required for activating pre-mRNA splicing. May have a scaffolding role in the spliceosome assembly as it contacts all other components of the core complex. The PRP19-CDC5L complex may also play a role in the response to DNA damage (DDR).
7 PublicationsGO - Biological processi
- mRNA splicing, via spliceosome Source: UniProtKB
- RNA splicing Source: UniProtKB
- RNA splicing, via transesterification reactions Source: UniProtKB
Keywordsi
Biological process | mRNA processing, mRNA splicing |
Enzyme and pathway databases
PathwayCommonsi | O75934 |
Reactomei | R-HSA-72163, mRNA Splicing - Major Pathway |
SignaLinki | O75934 |
Names & Taxonomyi
Protein namesi | Recommended name: Pre-mRNA-splicing factor SPF27Alternative name(s): Breast carcinoma-amplified sequence 2 DNA amplified in mammary carcinoma 1 protein Spliceosome-associated protein SPF 27 |
Gene namesi | Name:BCAS2 Synonyms:DAM11 Publication |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:975, BCAS2 |
MIMi | 605783, gene |
neXtProti | NX_O75934 |
VEuPathDBi | HostDB:ENSG00000116752 |
Subcellular locationi
Cytoskeleton
- centrosome Source: HPA
Nucleus
- catalytic step 2 spliceosome Source: GO_Central
- nuclear speck Source: HPA
- nucleolus Source: UniProtKB-SubCell
- nucleoplasm Source: Reactome
- nucleus Source: UniProtKB
- spliceosomal complex Source: MGI
- U2-type catalytic step 2 spliceosome Source: UniProtKB
Other locations
- Prp19 complex Source: ComplexPortal
Keywords - Cellular componenti
Nucleus, SpliceosomePathology & Biotechi
Organism-specific databases
DisGeNETi | 10286 |
OpenTargetsi | ENSG00000116752 |
PharmGKBi | PA25285 |
Miscellaneous databases
Pharosi | O75934, Tbio |
Genetic variation databases
BioMutai | BCAS2 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | RemovedCombined sources1 Publication | |||
ChainiPRO_0000064861 | 2 – 225 | Pre-mRNA-splicing factor SPF27Add BLAST | 224 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 2 | N-acetylalanineCombined sources1 Publication | 1 | |
Modified residuei | 94 | PhosphoserineCombined sources | 1 |
Keywords - PTMi
Acetylation, PhosphoproteinProteomic databases
EPDi | O75934 |
jPOSTi | O75934 |
MassIVEi | O75934 |
MaxQBi | O75934 |
PaxDbi | O75934 |
PeptideAtlasi | O75934 |
PRIDEi | O75934 |
ProteomicsDBi | 50297 |
TopDownProteomicsi | O75934 |
2D gel databases
SWISS-2DPAGEi | O75934 |
PTM databases
GlyGeni | O75934, 1 site, 1 O-linked glycan (1 site) |
iPTMneti | O75934 |
PhosphoSitePlusi | O75934 |
Expressioni
Tissue specificityi
Gene expression databases
Bgeei | ENSG00000116752, Expressed in frontal cortex and 250 other tissues |
ExpressionAtlasi | O75934, baseline and differential |
Genevisiblei | O75934, HS |
Organism-specific databases
HPAi | ENSG00000116752, Low tissue specificity |
Interactioni
Subunit structurei
Component of the pre-catalytic and catalytic spliceosome complexes (PubMed:9731529, PubMed:28502770, PubMed:28076346, PubMed:29360106, PubMed:29301961).
Component of the postcatalytic spliceosome P complex (PubMed:30705154).
Component of the PRP19-CDC5L splicing complex composed of a core complex comprising a homotetramer of PRPF19, CDC5L, PLRG1 and BCAS2, and at least three less stably associated proteins CTNNBL1, CWC15 and HSPA8.
Interacts directly in the complex with PRPF19, CDC5L and PLRG1 (PubMed:20176811).
7 PublicationsBinary interactionsi
O75934
Protein-protein interaction databases
BioGRIDi | 115575, 256 interactors |
ComplexPortali | CPX-5824, PRP19-CDC5L complex |
CORUMi | O75934 |
IntActi | O75934, 108 interactors |
MINTi | O75934 |
STRINGi | 9606.ENSP00000358554 |
Miscellaneous databases
RNActi | O75934, protein |
Structurei
Secondary structure
3D structure databases
AlphaFoldDBi | O75934 |
SMRi | O75934 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Coiled coil
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Coiled coili | 138 – 222 | Sequence analysisAdd BLAST | 85 |
Sequence similaritiesi
Keywords - Domaini
Coiled coilPhylogenomic databases
eggNOGi | KOG3096, Eukaryota |
GeneTreei | ENSGT00390000014494 |
HOGENOMi | CLU_082523_2_1_1 |
InParanoidi | O75934 |
OMAi | SHLSMRH |
OrthoDBi | 1607056at2759 |
PhylomeDBi | O75934 |
TreeFami | TF105818 |
Family and domain databases
InterProi | View protein in InterPro IPR008409, SPF27 |
PANTHERi | PTHR13296, PTHR13296, 1 hit |
Pfami | View protein in Pfam PF05700, BCAS2, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
10 20 30 40 50
MAGTGLVAGE VVVDALPYFD QGYEAPGVRE AAAALVEEET RRYRPTKNYL
60 70 80 90 100
SYLTAPDYSA FETDIMRNEF ERLAARQPIE LLSMKRYELP APSSGQKNDI
110 120 130 140 150
TAWQECVNNS MAQLEHQAVR IENLELMSQH GCNAWKVYNE NLVHMIEHAQ
160 170 180 190 200
KELQKLRKHI QDLNWQRKNM QLTAGSKLRE MESNWVSLVS KNYEIERTIV
210 220
QLENEIYQIK QQHGEANKEN IRQDF
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 24 | E → D in CAG46834 (Ref. 3) Curated | 1 | |
Sequence conflicti | 89 | L → V in CAG46834 (Ref. 3) Curated | 1 |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_035799 | 139 | N → S in a colorectal cancer sample; somatic mutation. 1 PublicationCorresponds to variant dbSNP:rs1197668726Ensembl. | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF081788 mRNA Translation: AAC64059.1 AB020623 mRNA Translation: BAA34863.1 CR542037 mRNA Translation: CAG46834.1 BT019390 mRNA Translation: AAV38197.1 AL390241 Genomic DNA No translation available. BC005285 mRNA Translation: AAH05285.1 BC012623 mRNA Translation: AAH12623.1 BC022880 mRNA Translation: AAH22880.1 |
CCDSi | CCDS874.1 |
RefSeqi | NP_005863.1, NM_005872.2 |
Genome annotation databases
Ensembli | ENST00000369541.4; ENSP00000358554.3; ENSG00000116752.6 |
GeneIDi | 10286 |
KEGGi | hsa:10286 |
MANE-Selecti | ENST00000369541.4; ENSP00000358554.3; NM_005872.3; NP_005863.1 |
UCSCi | uc001efa.4, human |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF081788 mRNA Translation: AAC64059.1 AB020623 mRNA Translation: BAA34863.1 CR542037 mRNA Translation: CAG46834.1 BT019390 mRNA Translation: AAV38197.1 AL390241 Genomic DNA No translation available. BC005285 mRNA Translation: AAH05285.1 BC012623 mRNA Translation: AAH12623.1 BC022880 mRNA Translation: AAH22880.1 |
CCDSi | CCDS874.1 |
RefSeqi | NP_005863.1, NM_005872.2 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
5MQF | electron microscopy | 5.90 | K | 1-225 | [»] | |
5XJC | electron microscopy | 3.60 | K | 1-225 | [»] | |
5YZG | electron microscopy | 4.10 | K | 1-225 | [»] | |
5Z56 | electron microscopy | 5.10 | K | 1-225 | [»] | |
5Z57 | electron microscopy | 6.50 | K | 1-225 | [»] | |
6FF7 | electron microscopy | 4.50 | K | 1-225 | [»] | |
6ICZ | electron microscopy | 3.00 | K | 1-225 | [»] | |
6ID0 | electron microscopy | 2.90 | K | 1-225 | [»] | |
6ID1 | electron microscopy | 2.86 | K | 1-225 | [»] | |
6QDV | electron microscopy | 3.30 | s | 1-225 | [»] | |
7A5P | electron microscopy | 5.00 | K | 1-225 | [»] | |
AlphaFoldDBi | O75934 | |||||
SMRi | O75934 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 115575, 256 interactors |
ComplexPortali | CPX-5824, PRP19-CDC5L complex |
CORUMi | O75934 |
IntActi | O75934, 108 interactors |
MINTi | O75934 |
STRINGi | 9606.ENSP00000358554 |
PTM databases
GlyGeni | O75934, 1 site, 1 O-linked glycan (1 site) |
iPTMneti | O75934 |
PhosphoSitePlusi | O75934 |
Genetic variation databases
BioMutai | BCAS2 |
2D gel databases
SWISS-2DPAGEi | O75934 |
Proteomic databases
EPDi | O75934 |
jPOSTi | O75934 |
MassIVEi | O75934 |
MaxQBi | O75934 |
PaxDbi | O75934 |
PeptideAtlasi | O75934 |
PRIDEi | O75934 |
ProteomicsDBi | 50297 |
TopDownProteomicsi | O75934 |
Protocols and materials databases
Antibodypediai | 33860, 346 antibodies from 33 providers |
DNASUi | 10286 |
Genome annotation databases
Ensembli | ENST00000369541.4; ENSP00000358554.3; ENSG00000116752.6 |
GeneIDi | 10286 |
KEGGi | hsa:10286 |
MANE-Selecti | ENST00000369541.4; ENSP00000358554.3; NM_005872.3; NP_005863.1 |
UCSCi | uc001efa.4, human |
Organism-specific databases
CTDi | 10286 |
DisGeNETi | 10286 |
GeneCardsi | BCAS2 |
HGNCi | HGNC:975, BCAS2 |
HPAi | ENSG00000116752, Low tissue specificity |
MIMi | 605783, gene |
neXtProti | NX_O75934 |
OpenTargetsi | ENSG00000116752 |
PharmGKBi | PA25285 |
VEuPathDBi | HostDB:ENSG00000116752 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG3096, Eukaryota |
GeneTreei | ENSGT00390000014494 |
HOGENOMi | CLU_082523_2_1_1 |
InParanoidi | O75934 |
OMAi | SHLSMRH |
OrthoDBi | 1607056at2759 |
PhylomeDBi | O75934 |
TreeFami | TF105818 |
Enzyme and pathway databases
PathwayCommonsi | O75934 |
Reactomei | R-HSA-72163, mRNA Splicing - Major Pathway |
SignaLinki | O75934 |
Miscellaneous databases
BioGRID-ORCSi | 10286, 633 hits in 1084 CRISPR screens |
ChiTaRSi | BCAS2, human |
GeneWikii | BCAS2 |
GenomeRNAii | 10286 |
Pharosi | O75934, Tbio |
PROi | PR:O75934 |
RNActi | O75934, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000116752, Expressed in frontal cortex and 250 other tissues |
ExpressionAtlasi | O75934, baseline and differential |
Genevisiblei | O75934, HS |
Family and domain databases
InterProi | View protein in InterPro IPR008409, SPF27 |
PANTHERi | PTHR13296, PTHR13296, 1 hit |
Pfami | View protein in Pfam PF05700, BCAS2, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | SPF27_HUMAN | |
Accessioni | O75934Primary (citable) accession number: O75934 Secondary accession number(s): Q6FGS0 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | May 10, 2005 |
Last sequence update: | November 1, 1998 | |
Last modified: | May 25, 2022 | |
This is version 183 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- Human chromosome 1
Human chromosome 1: entries, gene names and cross-references to MIM - Human entries with genetic variants
List of human entries with genetic variants - Human variants curated from literature reports
Index of human variants curated from literature reports - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families