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Protein

Pre-mRNA-splicing factor SPF27

Gene

BCAS2

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Component of the PRP19-CDC5L complex that forms an integral part of the spliceosome and is required for activating pre-mRNA splicing. May have a scaffolding role in the spliceosome assembly as it contacts all other components of the core complex. The PRP19-CDC5L complex may also play a role in the response to DNA damage (DDR).1 Publication

GO - Biological processi

  • mRNA splicing, via spliceosome Source: Reactome
  • RNA splicing Source: UniProtKB
  • RNA splicing, via transesterification reactions Source: UniProtKB

Keywordsi

Biological processmRNA processing, mRNA splicing

Enzyme and pathway databases

ReactomeiR-HSA-72163 mRNA Splicing - Major Pathway

Names & Taxonomyi

Protein namesi
Recommended name:
Pre-mRNA-splicing factor SPF27
Alternative name(s):
Breast carcinoma-amplified sequence 2
DNA amplified in mammary carcinoma 1 protein
Spliceosome-associated protein SPF 27
Gene namesi
Name:BCAS2
Synonyms:DAM1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 1

Organism-specific databases

EuPathDBiHostDB:ENSG00000116752.5
HGNCiHGNC:975 BCAS2
MIMi605783 gene
neXtProtiNX_O75934

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus, Spliceosome

Pathology & Biotechi

Organism-specific databases

DisGeNETi10286
OpenTargetsiENSG00000116752
PharmGKBiPA25285

Polymorphism and mutation databases

BioMutaiBCAS2

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources1 Publication
ChainiPRO_00000648612 – 225Pre-mRNA-splicing factor SPF27Add BLAST224

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineCombined sources1 Publication1
Modified residuei94PhosphoserineCombined sources1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiO75934
MaxQBiO75934
PaxDbiO75934
PeptideAtlasiO75934
PRIDEiO75934
ProteomicsDBi50297
TopDownProteomicsiO75934

2D gel databases

SWISS-2DPAGEiO75934

PTM databases

iPTMnetiO75934
PhosphoSitePlusiO75934

Miscellaneous databases

PMAP-CutDBiO75934

Expressioni

Tissue specificityi

Ubiquitously expressed.1 Publication

Gene expression databases

BgeeiENSG00000116752 Expressed in 238 organ(s), highest expression level in frontal cortex
CleanExiHS_BCAS2
ExpressionAtlasiO75934 baseline and differential
GenevisibleiO75934 HS

Organism-specific databases

HPAiHPA067881

Interactioni

Subunit structurei

Component of the PRP19-CDC5L splicing complex composed of a core complex comprising a homotetramer of PRPF19, CDC5L, PLRG1 and BCAS2, and at least three less stably associated proteins CTNNBL1, CWC15 and HSPA8. Interacts directly in the complex with PRPF19, CDC5L and PLRG1.2 Publications

Binary interactionsi

Protein-protein interaction databases

BioGridi115575, 111 interactors
CORUMiO75934
IntActiO75934, 74 interactors
MINTiO75934
STRINGi9606.ENSP00000358554

Structurei

3D structure databases

ProteinModelPortaliO75934
SMRiO75934
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili138 – 222Sequence analysisAdd BLAST85

Sequence similaritiesi

Belongs to the SPF27 family.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiKOG3096 Eukaryota
ENOG41101IB LUCA
GeneTreeiENSGT00390000014494
HOGENOMiHOG000007540
HOVERGENiHBG050675
InParanoidiO75934
KOiK12861
OMAiQNRLPME
OrthoDBiEOG091G0NMJ
PhylomeDBiO75934
TreeFamiTF105818

Family and domain databases

InterProiView protein in InterPro
IPR008409 SPF27
PANTHERiPTHR13296 PTHR13296, 1 hit
PfamiView protein in Pfam
PF05700 BCAS2, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O75934-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MAGTGLVAGE VVVDALPYFD QGYEAPGVRE AAAALVEEET RRYRPTKNYL
60 70 80 90 100
SYLTAPDYSA FETDIMRNEF ERLAARQPIE LLSMKRYELP APSSGQKNDI
110 120 130 140 150
TAWQECVNNS MAQLEHQAVR IENLELMSQH GCNAWKVYNE NLVHMIEHAQ
160 170 180 190 200
KELQKLRKHI QDLNWQRKNM QLTAGSKLRE MESNWVSLVS KNYEIERTIV
210 220
QLENEIYQIK QQHGEANKEN IRQDF
Length:225
Mass (Da):26,131
Last modified:November 1, 1998 - v1
Checksum:i9112718EEFD96890
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti24E → D in CAG46834 (Ref. 3) Curated1
Sequence conflicti89L → V in CAG46834 (Ref. 3) Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_035799139N → S in a colorectal cancer sample; somatic mutation. 1 PublicationCorresponds to variant dbSNP:rs1197668726Ensembl.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF081788 mRNA Translation: AAC64059.1
AB020623 mRNA Translation: BAA34863.1
CR542037 mRNA Translation: CAG46834.1
BT019390 mRNA Translation: AAV38197.1
AL390241 Genomic DNA No translation available.
BC005285 mRNA Translation: AAH05285.1
BC012623 mRNA Translation: AAH12623.1
BC022880 mRNA Translation: AAH22880.1
CCDSiCCDS874.1
RefSeqiNP_005863.1, NM_005872.2
UniGeneiHs.22960

Genome annotation databases

EnsembliENST00000369541; ENSP00000358554; ENSG00000116752
GeneIDi10286
KEGGihsa:10286
UCSCiuc001efa.4 human

Keywords - Coding sequence diversityi

Polymorphism

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF081788 mRNA Translation: AAC64059.1
AB020623 mRNA Translation: BAA34863.1
CR542037 mRNA Translation: CAG46834.1
BT019390 mRNA Translation: AAV38197.1
AL390241 Genomic DNA No translation available.
BC005285 mRNA Translation: AAH05285.1
BC012623 mRNA Translation: AAH12623.1
BC022880 mRNA Translation: AAH22880.1
CCDSiCCDS874.1
RefSeqiNP_005863.1, NM_005872.2
UniGeneiHs.22960

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5MQFelectron microscopy5.90K1-225[»]
5XJCelectron microscopy3.60K1-225[»]
5YZGelectron microscopy4.10K1-225[»]
5Z56electron microscopy5.10K1-225[»]
5Z57electron microscopy6.50K1-225[»]
ProteinModelPortaliO75934
SMRiO75934
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi115575, 111 interactors
CORUMiO75934
IntActiO75934, 74 interactors
MINTiO75934
STRINGi9606.ENSP00000358554

PTM databases

iPTMnetiO75934
PhosphoSitePlusiO75934

Polymorphism and mutation databases

BioMutaiBCAS2

2D gel databases

SWISS-2DPAGEiO75934

Proteomic databases

EPDiO75934
MaxQBiO75934
PaxDbiO75934
PeptideAtlasiO75934
PRIDEiO75934
ProteomicsDBi50297
TopDownProteomicsiO75934

Protocols and materials databases

DNASUi10286
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000369541; ENSP00000358554; ENSG00000116752
GeneIDi10286
KEGGihsa:10286
UCSCiuc001efa.4 human

Organism-specific databases

CTDi10286
DisGeNETi10286
EuPathDBiHostDB:ENSG00000116752.5
GeneCardsiBCAS2
H-InvDBiHIX0077639
HGNCiHGNC:975 BCAS2
HPAiHPA067881
MIMi605783 gene
neXtProtiNX_O75934
OpenTargetsiENSG00000116752
PharmGKBiPA25285
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG3096 Eukaryota
ENOG41101IB LUCA
GeneTreeiENSGT00390000014494
HOGENOMiHOG000007540
HOVERGENiHBG050675
InParanoidiO75934
KOiK12861
OMAiQNRLPME
OrthoDBiEOG091G0NMJ
PhylomeDBiO75934
TreeFamiTF105818

Enzyme and pathway databases

ReactomeiR-HSA-72163 mRNA Splicing - Major Pathway

Miscellaneous databases

ChiTaRSiBCAS2 human
GeneWikiiBCAS2
GenomeRNAii10286
PMAP-CutDBiO75934
PROiPR:O75934
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000116752 Expressed in 238 organ(s), highest expression level in frontal cortex
CleanExiHS_BCAS2
ExpressionAtlasiO75934 baseline and differential
GenevisibleiO75934 HS

Family and domain databases

InterProiView protein in InterPro
IPR008409 SPF27
PANTHERiPTHR13296 PTHR13296, 1 hit
PfamiView protein in Pfam
PF05700 BCAS2, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiSPF27_HUMAN
AccessioniPrimary (citable) accession number: O75934
Secondary accession number(s): Q6FGS0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 10, 2005
Last sequence update: November 1, 1998
Last modified: November 7, 2018
This is version 162 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Human chromosome 1
    Human chromosome 1: entries, gene names and cross-references to MIM
  3. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  4. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  5. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  6. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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