PIAS2

Functionⁱ

Functions as an E3-type small ubiquitin-like modifier (SUMO) ligase, stabilizing the interaction between UBE2I and the substrate, and as a SUMO-tethering factor. Plays a crucial role as a transcriptional coregulator in various cellular pathways, including the STAT pathway, the p53 pathway and the steroid hormone signaling pathway. The effects of this transcriptional coregulation, transactivation or silencing may vary depending upon the biological context and the PIAS2 isoform studied. However, it seems to be mostly involved in gene silencing. Binds to sumoylated ELK1 and enhances its transcriptional activity by preventing recruitment of HDAC2 by ELK1, thus reversing SUMO-mediated repression of ELK1 transactivation activity. Isoform PIAS2-beta, but not isoform PIAS2-alpha, promotes MDM2 sumoylation. Isoform PIAS2-alpha promotes PARK7 sumoylation. Isoform PIAS2-beta promotes NCOA2 sumoylation more efficiently than isoform PIAS2-alpha. Isoform PIAS2-alpha sumoylates PML at'Lys-65' and 'Lys-160'.3 Publications

Pathwayⁱ: protein sumoylation

This protein is involved in the pathway protein sumoylation, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein sumoylation and in Protein modification.

Regions

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Zinc fingerⁱ	331 – 408	SP-RING-typePROSITE-ProRule annotationAdd BLAST		78

GO - Molecular functionⁱ

androgen receptor binding
Source: UniProtKB
Non-traceable author statementⁱ
- 15572661
DNA binding Source: UniProtKB-KW
SUMO ligase activity
Source: ParkinsonsUK-UCL
Inferred from direct assayⁱ
- 20408817
SUMO transferase activity
Source: Reactome
Inferred from experimentⁱ
transcription coactivator activity
Source: UniProtKB
Non-traceable author statementⁱ
- 15572661
transcription factor binding
Source: ParkinsonsUK-UCL
Inferred from physical interactionⁱ
- 20408817
ubiquitin protein ligase binding Source: Ensembl
zinc ion binding
Source: ProtInc
Traceable author statementⁱ
- 9256341

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

androgen receptor signaling pathway
Source: UniProtKB
Non-traceable author statementⁱ
- 15572661
negative regulation of androgen receptor signaling pathway
Source: UniProtKB
Inferred from direct assayⁱ
- 11477070
negative regulation of DNA-binding transcription factor activity
Source: ParkinsonsUK-UCL
Inferred from direct assayⁱ
- 20408817
positive regulation of transcription, DNA-templated
Source: UniProtKB
Non-traceable author statementⁱ
- 15572661
protein sumoylation
Source: ParkinsonsUK-UCL
Inferred from direct assayⁱ
- 20408817

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Molecular function	DNA-binding, Transferase
Biological process	Transcription, Transcription regulation, Ubl conjugation pathway
Ligand	Metal-binding, Zinc

Enzyme and pathway databases

Reactomeⁱ	R-HSA-3108214 SUMOylation of DNA damage response and repair proteins R-HSA-3232118 SUMOylation of transcription factors R-HSA-3232142 SUMOylation of ubiquitinylation proteins R-HSA-3899300 SUMOylation of transcription cofactors R-HSA-4090294 SUMOylation of intracellular receptors R-HSA-4551638 SUMOylation of chromatin organization proteins R-HSA-5617472 Activation of anterior HOX genes in hindbrain development during early embryogenesis
SignaLinkⁱ	O75928
UniPathwayⁱ	UPA00886

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: E3 SUMO-protein ligase PIAS2 (EC:2.3.2.-) Alternative name(s): Androgen receptor-interacting protein 3 Short name: ARIP3 DAB2-interacting protein Short name: DIP E3 SUMO-protein transferase PIAS2Curated Msx-interacting zinc finger protein Short name: Miz1 PIAS-NY protein Protein inhibitor of activated STAT x Protein inhibitor of activated STAT2
Gene namesⁱ	Name:PIAS2 Synonyms:PIASX
Organismⁱ	Homo sapiens (Human)
Taxonomic identifierⁱ	9606 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Dipnotetrapodomorpha › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Boreoeutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo
Proteomesⁱ	UP000005640 Componentⁱ: Chromosome 18

Organism-specific databases

EuPathDBⁱ	HostDB:ENSG00000078043.15
Human Gene Nomenclature Database More...HGNCⁱ	HGNC:17311 PIAS2
MIMⁱ	603567 gene
neXtProtⁱ	NX_O75928

Keywords - Cellular componentⁱ

Nucleus

Pathology & Biotechⁱ

Mutagenesis

Feature key	Position(s)	DescriptionActions	Length
Mutagenesisⁱ	362	C → S or A: Loss of MDM2 and TP53 sumoylation and of autosumoylation; no loss of JUN- and TP53-binding. 2 Publications	1
Mutagenesisⁱ	467	V → A: Reduces affinity for SUMO1. 1 Publication	1
Mutagenesisⁱ	469	V → A: Abolishes binding to SUMO1. 1 Publication	1
Mutagenesisⁱ	470	I → A: Abolishes binding to SUMO1. 1 Publication	1
Mutagenesisⁱ	472	L → A: Abolishes binding to SUMO1. 1 Publication	1
Mutagenesisⁱ	473	T → A: Reduces affinity for SUMO1. 1 Publication	1

Organism-specific databases

DisGeNET More...DisGeNETⁱ	9063
Open Targets More...OpenTargetsⁱ	ENSG00000078043
PharmGKBⁱ	PA134933292

Polymorphism and mutation databases

BioMutaⁱ	PIAS2

BioMutaⁱ

PIAS2

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
ChainⁱPRO_0000218976	1 – 621	E3 SUMO-protein ligase PIAS2Add BLAST		621

Amino acid modifications

Feature key	Position(s)	DescriptionActions	Length
Cross-linkⁱ	46	Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Cross-linkⁱ	249	Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Cross-linkⁱ	430	Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Cross-linkⁱ	435	Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Cross-linkⁱ	443	Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Cross-linkⁱ	452	Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Modified residueⁱ	476	PhosphoserineCombined sources	1
Modified residueⁱ	477	PhosphoserineCombined sources	1
Modified residueⁱ	478	PhosphoserineCombined sources	1
Cross-linkⁱ	489	Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Modified residueⁱ	499	PhosphoserineBy similarity	1
Cross-linkⁱ	502	Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Isoform PIAS2-alpha (identifier: O75928-2)
Cross-linkⁱ	562	Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources

Post-translational modificationⁱ

Sumoylated.2 Publications

Keywords - PTMⁱ

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDⁱ	O75928
MaxQB - The MaxQuant DataBase More...MaxQBⁱ	O75928
PaxDbⁱ	O75928
PeptideAtlas More...PeptideAtlasⁱ	O75928
PRIDEⁱ	O75928
ProteomicsDBⁱ	50294 50295 [O75928-2] 50296 [O75928-3]

PTM databases

iPTMnetⁱ	O75928
PhosphoSitePlusⁱ	O75928

Expressionⁱ

Tissue specificityⁱ

Mainly expressed in testis. Isoform 3 is expressed predominantly in adult testis, weakly in pancreas, embryonic testis and sperm, and at very low levels in other organs.3 Publications

Developmental stageⁱ

Isoform 3 expression in adult testis is 14.2-fold stronger than in embryonic testis.1 Publication

Inductionⁱ

Up-regulated transiently during myeloid differentiation in various cells lines, such as HL-60, U-937, K-562, induced by either phorbol ester (TPA) or retinoic acid.

Gene expression databases

Bgeeⁱ	ENSG00000078043 Expressed in 226 organ(s), highest expression level in testis
CleanExⁱ	HS_PIAS2
ExpressionAtlasⁱ	O75928 baseline and differential
Genevisibleⁱ	O75928 HS

Organism-specific databases

Human Protein Atlas More...HPAⁱ	HPA068792

HPAⁱ

HPA068792

Interactionⁱ

Subunit structureⁱ

Binds SUMO1 and UBE2I. Interacts with AXIN1, JUN, MDM2, PARK7, TP53 and TP73 isoform alpha, but not TP73 isoform beta. Interacts with STAT4 following IL12 and IFN-alpha stimulation of T-cells. Interacts also with GTF2I, GTF2IRD1, IKFZ1, DAB2 and MSX2, as well as with several steroid receptors, including ESR1, ESR2, NR3C1, PGR, AR, and with NCOA2 (By similarity). Sumoylation of a target protein seems to enhance the interaction. Binds to sumoylated ELK1. Binds DNA, such as CDKN1A promoter, in a sequence-specific manner. Interacts with PLAG1. Interacts with KLF8; the interaction results in SUMO ligation and repression of KLF8 transcriptional activity and of its cell cycle progression into G₁ phase. PIAS2-beta interacts with IFIH1/MDA5. Isoform PIAS2-alpha interacts with PML (isoform PML-12).By similarity11 Publications

Binary interactionsⁱ

With	Entry	#Exp.	IntAct
	G2XKQ0	3	EBI-348555,EBI-10175576
	Q59GP6	3	EBI-348555,EBI-10243413
ACY3	Q96HD9	3	EBI-348555,EBI-3916242
CCHCR1	Q8TD31-3	3	EBI-348555,EBI-10175300
CCNDBP1	O95273	4	EBI-348555,EBI-748961
GFAP	P14136	5	EBI-348567,EBI-744302
GOLGA2	Q08379	3	EBI-348555,EBI-618309
MX2	P20592	3	EBI-348555,EBI-10200618
MYC	P01106	4	EBI-348555,EBI-447544
NAV2	Q8IVL1	4	EBI-348555,EBI-741200
PRKAB2	O43741	3	EBI-348555,EBI-1053424
RACK1	P63244	2	EBI-348555,EBI-296739
RUFY1	Q96T51	3	EBI-348555,EBI-3941207
SUMO1	P63165	6	EBI-348555,EBI-80140
TP53	P04637	2	EBI-348555,EBI-366083
TRIM23	P36406	3	EBI-348555,EBI-740098
TSR2	Q969E8	3	EBI-348555,EBI-746981
UBE2I	P63279	8	EBI-348555,EBI-80168
UBE2I	Q7KZS0	3	EBI-348555,EBI-10180829
ZBED1	O96006	3	EBI-348555,EBI-740037
ZBTB8A	Q96BR9	3	EBI-348555,EBI-742740

GO - Molecular functionⁱ

androgen receptor binding
Source: UniProtKB
Non-traceable author statementⁱ
- 15572661
transcription factor binding
Source: ParkinsonsUK-UCL
Inferred from physical interactionⁱ
- 20408817
ubiquitin protein ligase binding Source: Ensembl

View the complete GO annotation on QuickGO ...

Protein-protein interaction databases

BioGridⁱ	114523, 77 interactors
ELMⁱ	O75928
IntActⁱ	O75928, 100 interactors
Molecular INTeraction database More...MINTⁱ	O75928
STRINGⁱ	9606.ENSP00000465676

Structureⁱ

Secondary structure

Legend: HelixTurnBeta strandPDB Structure known for this area

Feature key	Position(s)	DescriptionActions	Length
Beta strandⁱ	154 – 165	Combined sources	12
Beta strandⁱ	173 – 181	Combined sources	9
Helixⁱ	185 – 192	Combined sources	8
Beta strandⁱ	195 – 197	Combined sources	3
Beta strandⁱ	203 – 215	Combined sources	13
Beta strandⁱ	231 – 234	Combined sources	4
Beta strandⁱ	237 – 239	Combined sources	3
Helixⁱ	264 – 266	Combined sources	3
Beta strandⁱ	271 – 273	Combined sources	3
Beta strandⁱ	275 – 282	Combined sources	8
Beta strandⁱ	289 – 298	Combined sources	10
Helixⁱ	301 – 309	Combined sources	9
Helixⁱ	316 – 327	Combined sources	12
Beta strandⁱ	340 – 345	Combined sources	6
Turnⁱ	347 – 349	Combined sources	3
Beta strandⁱ	354 – 359	Combined sources	6
Helixⁱ	370 – 379	Combined sources	10
Turnⁱ	386 – 388	Combined sources	3
Helixⁱ	394 – 396	Combined sources	3
Beta strandⁱ	397 – 400	Combined sources	4
Helixⁱ	401 – 407	Combined sources	7
Beta strandⁱ	415 – 418	Combined sources	4
Beta strandⁱ	469 – 471	Combined sources	3

Show more details

3D structure databases

ProteinModelPortalⁱ	O75928
SMRⁱ	O75928
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Miscellaneous databases

EvolutionaryTraceⁱ	O75928

EvolutionaryTraceⁱ

O75928

Family & Domainsⁱ

Domains and Repeats

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Domainⁱ	11 – 45	SAPPROSITE-ProRule annotationAdd BLAST		35
Domainⁱ	134 – 299	PINITPROSITE-ProRule annotationAdd BLAST		166

Region

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Regionⁱ	467 – 473	SUMO1-binding		7

Motif

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Motifⁱ	19 – 23	LXXLL motif		5
Motifⁱ	484 – 492	Nuclear localization signalBy similarity		9

Compositional bias

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Compositional biasⁱ	571 – 612	Ser-richAdd BLAST		42
Compositional biasⁱ	596 – 602	Poly-Ser		7

Domainⁱ

The LXXLL motif is a transcriptional coregulator signature.

Sequence similaritiesⁱ

Belongs to the PIAS family.Curated

Zinc finger

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Zinc fingerⁱ	331 – 408	SP-RING-typePROSITE-ProRule annotationAdd BLAST		78

Keywords - Domainⁱ

Zinc-finger

Phylogenomic databases

eggNOGⁱ	KOG2169 Eukaryota ENOG410XQ2E LUCA
Ensembl GeneTree More...GeneTreeⁱ	ENSGT00550000074410
HOGENOMⁱ	HOG000230594
HOVERGENⁱ	HBG053598
InParanoidⁱ	O75928
KEGG Orthology (KO) More...KOⁱ	K16063
OMAⁱ	DPQQYCP
Database of Orthologous Groups More...OrthoDBⁱ	EOG091G08G5
PhylomeDBⁱ	O75928

Family and domain databases

Gene3Dⁱ	1.10.720.30, 1 hit 2.60.120.780, 1 hit 3.30.40.10, 1 hit
InterProⁱ	View protein in InterPro IPR027228 PIAS2 IPR023321 PINIT IPR038654 PINIT_sf IPR003034 SAP_dom IPR036361 SAP_dom_sf IPR004181 Znf_MIZ IPR013083 Znf_RING/FYVE/PHD
PANTHERⁱ	PTHR10782:SF12 PTHR10782:SF12, 1 hit
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF14324 PINIT, 1 hit PF02891 zf-MIZ, 1 hit
SMARTⁱ	View protein in SMART SM00513 SAP, 1 hit
SUPFAMⁱ	SSF68906 SSF68906, 1 hit
PROSITEⁱ	View protein in PROSITE PS51466 PINIT, 1 hit PS50800 SAP, 1 hit PS51044 ZF_SP_RING, 1 hit

Sequences (3+)ⁱ

Sequence statusⁱ: Complete.

This entry describes 3 isoformsⁱ produced by alternative splicing. Align Add to basket

This entry has 3 described isoforms and 7 potential isoforms that are computationally mapped.Show all Align All

Isoform PIAS2-beta (identifier: O75928-1) [UniParc]FASTA Add to basket

Also known as: PIASx-beta, Miz1

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MADFEELRNM VSSFRVSELQ VLLGFAGRNK SGRKHDLLMR ALHLLKSGCS 
        60         70         80         90        100
PAVQIKIREL YRRRYPRTLE GLSDLSTIKS SVFSLDGGSS PVEPDLAVAG 
       110        120        130        140        150
IHSLPSTSVT PHSPSSPVGS VLLQDTKPTF EMQQPSPPIP PVHPDVQLKN 
       160        170        180        190        200
LPFYDVLDVL IKPTSLVQSS IQRFQEKFFI FALTPQQVRE ICISRDFLPG 
       210        220        230        240        250
GRRDYTVQVQ LRLCLAETSC PQEDNYPNSL CIKVNGKLFP LPGYAPPPKN 
       260        270        280        290        300
GIEQKRPGRP LNITSLVRLS SAVPNQISIS WASEIGKNYS MSVYLVRQLT 
       310        320        330        340        350
SAMLLQRLKM KGIRNPDHSR ALIKEKLTAD PDSEIATTSL RVSLMCPLGK 
       360        370        380        390        400
MRLTIPCRAV TCTHLQCFDA ALYLQMNEKK PTWICPVCDK KAAYESLILD 
       410        420        430        440        450
GLFMEILNDC SDVDEIKFQE DGSWCPMRPK KEAMKVSSQP CTKIESSSVL 
       460        470        480        490        500
SKPCSVTVAS EASKKKVDVI DLTIESSSDE EEDPPAKRKC IFMSETQSSP 
       510        520        530        540        550
TKGVLMYQPS SVRVPSVTSV DPAAIPPSLT DYSVPFHHTP ISSMSSDLPG 
       560        570        580        590        600
LDFLSLIPVD PQYCPPMFLD SLTSPLTASS TSVTTTSSHE SSTHVSSSSS 
       610        620 
RSETGVITSS GSNIPDIISL D

Length:621

Mass (Da):68,240

Last modified:May 5, 2009 - v3

Checksum:ⁱ6422B383345AD883

GO

Isoform PIAS2-alpha (identifier: O75928-2) [UniParc]FASTA Add to basket

Also known as: PIASx-alpha, ARIP3

The sequence of this isoform differs from the canonical sequence as follows:
551-572: LDFLSLIPVDPQYCPPMFLDSL → EQRRNDINNELKLGTSSDTVQQ
573-621: Missing.

« Hide

        10         20         30         40         50
MADFEELRNM VSSFRVSELQ VLLGFAGRNK SGRKHDLLMR ALHLLKSGCS 
        60         70         80         90        100
PAVQIKIREL YRRRYPRTLE GLSDLSTIKS SVFSLDGGSS PVEPDLAVAG 
       110        120        130        140        150
IHSLPSTSVT PHSPSSPVGS VLLQDTKPTF EMQQPSPPIP PVHPDVQLKN 
       160        170        180        190        200
LPFYDVLDVL IKPTSLVQSS IQRFQEKFFI FALTPQQVRE ICISRDFLPG 
       210        220        230        240        250
GRRDYTVQVQ LRLCLAETSC PQEDNYPNSL CIKVNGKLFP LPGYAPPPKN 
       260        270        280        290        300
GIEQKRPGRP LNITSLVRLS SAVPNQISIS WASEIGKNYS MSVYLVRQLT 
       310        320        330        340        350
SAMLLQRLKM KGIRNPDHSR ALIKEKLTAD PDSEIATTSL RVSLMCPLGK 
       360        370        380        390        400
MRLTIPCRAV TCTHLQCFDA ALYLQMNEKK PTWICPVCDK KAAYESLILD 
       410        420        430        440        450
GLFMEILNDC SDVDEIKFQE DGSWCPMRPK KEAMKVSSQP CTKIESSSVL 
       460        470        480        490        500
SKPCSVTVAS EASKKKVDVI DLTIESSSDE EEDPPAKRKC IFMSETQSSP 
       510        520        530        540        550
TKGVLMYQPS SVRVPSVTSV DPAAIPPSLT DYSVPFHHTP ISSMSSDLPG 
       560        570 
EQRRNDINNE LKLGTSSDTV QQ

Show »

Length:572

Mass (Da):63,396

Checksum:ⁱ63106D68C585F0ED

GO

Isoform 3 (identifier: O75928-3) [UniParc]FASTA Add to basket

The sequence of this isoform differs from the canonical sequence as follows:
1-8: MADFEELR → MNAGKQLQRTLH
402-621: Missing.

« Hide

        10         20         30         40         50
MNAGKQLQRT LHNMVSSFRV SELQVLLGFA GRNKSGRKHD LLMRALHLLK 
        60         70         80         90        100
SGCSPAVQIK IRELYRRRYP RTLEGLSDLS TIKSSVFSLD GGSSPVEPDL 
       110        120        130        140        150
AVAGIHSLPS TSVTPHSPSS PVGSVLLQDT KPTFEMQQPS PPIPPVHPDV 
       160        170        180        190        200
QLKNLPFYDV LDVLIKPTSL VQSSIQRFQE KFFIFALTPQ QVREICISRD 
       210        220        230        240        250
FLPGGRRDYT VQVQLRLCLA ETSCPQEDNY PNSLCIKVNG KLFPLPGYAP 
       260        270        280        290        300
PPKNGIEQKR PGRPLNITSL VRLSSAVPNQ ISISWASEIG KNYSMSVYLV 
       310        320        330        340        350
RQLTSAMLLQ RLKMKGIRNP DHSRALIKEK LTADPDSEIA TTSLRVSLMC 
       360        370        380        390        400
PLGKMRLTIP CRAVTCTHLQ CFDAALYLQM NEKKPTWICP VCDKKAAYES 

LILDG

Note: No experimental confirmation available.

Show »

Length:405

Mass (Da):45,057

Checksum:ⁱ6C16003BCDB6384E

GO

Computationally mapped potential isoform sequencesⁱ

There are 7 potential isoforms mapped to this entry.BLAST Align Show all Add to basket

Entry	Entry name	Protein names	Gene names	Length	Annotation

B4DGW0	B4DGW0_HUMAN	E3 SUMO-protein ligase PIAS2 E3 SUMO-protein ligase PIAS2 (cDNA FLJ57366, highly similar to Protein inhibitor of activated STAT2)	PIAS2	282	Annotation score:
A0A087WUY7	A0A087WUY7_HUMAN	E3 SUMO-protein ligase PIAS2 E3 SUMO-protein ligase PIAS2	PIAS2	536	Annotation score:
K7EMP6	K7EMP6_HUMAN	E3 SUMO-protein ligase PIAS2 E3 SUMO-protein ligase PIAS2	PIAS2	89	Annotation score:
A0A0A0MS92	A0A0A0MS92_HUMAN	E3 SUMO-protein ligase PIAS2 E3 SUMO-protein ligase PIAS2	PIAS2	182	Annotation score:
K7ER97	K7ER97_HUMAN	E3 SUMO-protein ligase PIAS2 E3 SUMO-protein ligase PIAS2	PIAS2	88	Annotation score:
K7EJT4	K7EJT4_HUMAN	E3 SUMO-protein ligase PIAS2 E3 SUMO-protein ligase PIAS2	PIAS2	122	Annotation score:
K7EQX5	K7EQX5_HUMAN	E3 SUMO-protein ligase PIAS2 E3 SUMO-protein ligase PIAS2	PIAS2	95	Annotation score:

Experimental Info

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Sequence conflictⁱ	144	P → L in AAK48938 (PubMed:15301740).Curated		1
Sequence conflictⁱ	616	D → E in AAC36705 (PubMed:9724754).Curated		1

Natural variant

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Natural variantⁱVAR_056693	207	V → A. Corresponds to variant dbSNP:rs16940108Ensembl.		1

Alternative sequence

Feature key	Position(s)	DescriptionActions	Length
Alternative sequenceⁱVSP_012196	1 – 8	MADFEELR → MNAGKQLQRTLH in isoform 3. 1 Publication	8
Alternative sequenceⁱVSP_012197	402 – 621	Missing in isoform 3. 1 PublicationAdd BLAST	220
Alternative sequenceⁱVSP_050008	551 – 572	LDFLS…FLDSL → EQRRNDINNELKLGTSSDTV QQ in isoform PIAS2-alpha. 2 PublicationsAdd BLAST	22
Alternative sequenceⁱVSP_050009	573 – 621	Missing in isoform PIAS2-alpha. 2 PublicationsAdd BLAST	49

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AF077953 mRNA Translation: AAC36704.1 AF077954 mRNA Translation: AAC36705.1 AF361054 mRNA Translation: AAK48938.1 AC090241 Genomic DNA No translation available. AC090373 Genomic DNA No translation available. BC015190 mRNA Translation: AAH15190.1
The Consensus CDS (CCDS) project More...CCDSⁱ	CCDS32824.1 [O75928-1] CCDS32825.1 [O75928-2]
NCBI Reference Sequences More...RefSeqⁱ	NP_001310989.1, NM_001324060.1 [O75928-3] NP_004662.2, NM_004671.4 [O75928-1] NP_775298.1, NM_173206.3 [O75928-2]
UniGeneⁱ	Hs.57769 Hs.657844

Genome annotation databases

Ensemblⁱ	ENST00000324794; ENSP00000317163; ENSG00000078043 [O75928-2] ENST00000585916; ENSP00000465676; ENSG00000078043 [O75928-1]
GeneIDⁱ	9063
KEGGⁱ	hsa:9063
UCSC genome browser More...UCSCⁱ	uc002lck.4 human [O75928-1]

Keywords - Coding sequence diversityⁱ

Alternative splicing, Polymorphism

Similar proteinsⁱ

100% Identity
90% Identity
50% Identity

Protein	Similar proteins		Species	Score	Length	Source
O75928	PIAS2 isoform 1		PANTR		621	UniRef100_O75928
O75928-2	PIAS2 isoform 3		PANTR		572	UniRef100_O75928-2

Protein	Similar proteins		Species	Score	Length	Source
O75928	E3 SUMO-protein ligase PIAS2		MOUSE		621	UniRef90_O75928
PIAS2 isoform 1		PANTR		621
Uncharacterized protein		RABIT		738
E3 SUMO-protein ligase PIAS2 isoform X1		PHYCD		638
Uncharacterized protein		SAIBB		624
+315
O75928-2	PIAS2 isoform 3		PANTR		572	UniRef90_O75928-2
E3 SUMO-protein ligase PIAS2		AMAAE		614
Uncharacterized protein		CHLSB		572
E3 SUMO-protein ligase PIAS2 isoform X3		Florida manatee		572
E3 SUMO-protein ligase PIAS2 isoform X4		Enhydra lutris kenyoni		572
+51

Protein	Similar proteins		Species	Score	Length	Source
O75928	E3 SUMO-protein ligase PIAS2		MOUSE		621	UniRef50_O75928
PIAS2 isoform 1		PANTR		621
Uncharacterized protein		RABIT		738
E3 SUMO-protein ligase PIAS2 isoform X1		PHYCD		638
Uncharacterized protein		SAIBB		624
+740
O75928-2	E3 SUMO-protein ligase PIAS3		MOUSE		628	UniRef50_O75928-2
E3 SUMO-protein ligase PIAS3	)	HUMAN		628
E3 SUMO-protein ligase PIAS3		RAT		628
PIAS2 isoform 3		PANTR		572
E3 SUMO-protein ligase PIAS2		AMAAE		614
+1094

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AF077953 mRNA Translation: AAC36704.1 AF077954 mRNA Translation: AAC36705.1 AF361054 mRNA Translation: AAK48938.1 AC090241 Genomic DNA No translation available. AC090373 Genomic DNA No translation available. BC015190 mRNA Translation: AAH15190.1
CCDSⁱ	CCDS32824.1 [O75928-1] CCDS32825.1 [O75928-2]
RefSeqⁱ	NP_001310989.1, NM_001324060.1 [O75928-3] NP_004662.2, NM_004671.4 [O75928-1] NP_775298.1, NM_173206.3 [O75928-2]
UniGeneⁱ	Hs.57769 Hs.657844

3D structure databases

Select the link destinations: Protein Data Bank Europe More...PDBeⁱ Protein Data Bank RCSB More...RCSB PDBⁱ Protein Data Bank Japan More...PDBjⁱ Links Updated	PDB entry	Method	Resolution (Å)	Chain	Positions	PDBsum
	2ASQ	NMR	-	B	466-488	[»]
	4FO9	X-ray	2.39	A	147-488	[»]
ProteinModelPortalⁱ	O75928
SMRⁱ	O75928
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

BioGridⁱ	114523, 77 interactors
ELMⁱ	O75928
IntActⁱ	O75928, 100 interactors
MINTⁱ	O75928
STRINGⁱ	9606.ENSP00000465676

PTM databases

iPTMnetⁱ	O75928
PhosphoSitePlusⁱ	O75928

Polymorphism and mutation databases

BioMutaⁱ	PIAS2

BioMutaⁱ

PIAS2

Proteomic databases

EPDⁱ	O75928
MaxQBⁱ	O75928
PaxDbⁱ	O75928
PeptideAtlasⁱ	O75928
PRIDEⁱ	O75928
ProteomicsDBⁱ	50294 50295 [O75928-2] 50296 [O75928-3]

Protocols and materials databases

The DNASU plasmid repository More...DNASUⁱ	9063
Structural Biology Knowledgebase	Search...

Genome annotation databases

Ensemblⁱ	ENST00000324794; ENSP00000317163; ENSG00000078043 [O75928-2] ENST00000585916; ENSP00000465676; ENSG00000078043 [O75928-1]
GeneIDⁱ	9063
KEGGⁱ	hsa:9063
UCSCⁱ	uc002lck.4 human [O75928-1]

Organism-specific databases

CTDⁱ	9063
DisGeNETⁱ	9063
EuPathDBⁱ	HostDB:ENSG00000078043.15
GeneCardsⁱ	PIAS2
HGNCⁱ	HGNC:17311 PIAS2
HPAⁱ	HPA068792
MIMⁱ	603567 gene
neXtProtⁱ	NX_O75928
OpenTargetsⁱ	ENSG00000078043
PharmGKBⁱ	PA134933292
GenAtlas: human gene database More...GenAtlasⁱ	Search...

Phylogenomic databases

eggNOGⁱ	KOG2169 Eukaryota ENOG410XQ2E LUCA
GeneTreeⁱ	ENSGT00550000074410
HOGENOMⁱ	HOG000230594
HOVERGENⁱ	HBG053598
InParanoidⁱ	O75928
KOⁱ	K16063
OMAⁱ	DPQQYCP
OrthoDBⁱ	EOG091G08G5
PhylomeDBⁱ	O75928

Enzyme and pathway databases

UniPathwayⁱ	UPA00886
Reactomeⁱ	R-HSA-3108214 SUMOylation of DNA damage response and repair proteins R-HSA-3232118 SUMOylation of transcription factors R-HSA-3232142 SUMOylation of ubiquitinylation proteins R-HSA-3899300 SUMOylation of transcription cofactors R-HSA-4090294 SUMOylation of intracellular receptors R-HSA-4551638 SUMOylation of chromatin organization proteins R-HSA-5617472 Activation of anterior HOX genes in hindbrain development during early embryogenesis
SignaLinkⁱ	O75928

Miscellaneous databases

ChiTaRSⁱ	PIAS2 human
EvolutionaryTraceⁱ	O75928
GeneWikiⁱ	Protein_inhibitor_of_activated_STAT2
GenomeRNAiⁱ	9063
Protein Ontology More...PROⁱ	PR:O75928
SOURCEⁱ	Search...

Gene expression databases

Bgeeⁱ	ENSG00000078043 Expressed in 226 organ(s), highest expression level in testis
CleanExⁱ	HS_PIAS2
ExpressionAtlasⁱ	O75928 baseline and differential
Genevisibleⁱ	O75928 HS

Family and domain databases

Gene3Dⁱ	1.10.720.30, 1 hit 2.60.120.780, 1 hit 3.30.40.10, 1 hit
InterProⁱ	View protein in InterPro IPR027228 PIAS2 IPR023321 PINIT IPR038654 PINIT_sf IPR003034 SAP_dom IPR036361 SAP_dom_sf IPR004181 Znf_MIZ IPR013083 Znf_RING/FYVE/PHD
PANTHERⁱ	PTHR10782:SF12 PTHR10782:SF12, 1 hit
Pfamⁱ	View protein in Pfam PF14324 PINIT, 1 hit PF02891 zf-MIZ, 1 hit
SMARTⁱ	View protein in SMART SM00513 SAP, 1 hit
SUPFAMⁱ	SSF68906 SSF68906, 1 hit
PROSITEⁱ	View protein in PROSITE PS51466 PINIT, 1 hit PS50800 SAP, 1 hit PS51044 ZF_SP_RING, 1 hit
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	PIAS2_HUMAN
Accessionⁱ	O75928Primary (citable) accession number: O75928 Secondary accession number(s): O75927, Q96BT5, Q96KE3
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	December 7, 2004
	Last sequence update:	May 5, 2009
	Last modified:	November 7, 2018
	This is version 179 of the entry and version 3 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Chordata Protein Annotation Program
Disclaimer	Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousⁱ

Keywords - Technical termⁱ

3D-structure, Complete proteome, Reference proteome

Documents

SIMILARITY comments
Index of protein domains and families
Human entries with polymorphisms or disease mutations
List of human entries with polymorphisms or disease mutations
Human polymorphisms and disease mutations
Index of human polymorphisms and disease mutations
MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
PATHWAY comments
Index of metabolic and biosynthesis pathways
Human chromosome 18
Human chromosome 18: entries, gene names and cross-references to MIM
PDB cross-references
Index of Protein Data Bank (PDB) cross-references

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UniProtKB - O75928 (PIAS2_HUMAN)

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E3 SUMO-protein ligase PIAS2

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<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

<p>This subsection of the <a href="http://www.uniprot.org/help/function_section">'Function'</a> section describes the metabolic pathway(s) associated with a protein.<p><a href='/help/pathway' target='_top'>More...</a></p>Pathwayi: protein sumoylation

Regions

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

Enzyme and pathway databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

Organism-specific databases

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

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Nucleus

Keywords - Cellular componenti

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

Mutagenesis

Organism-specific databases

Polymorphism and mutation databases

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Amino acid modifications

Keywords - PTMi

Proteomic databases

PTM databases

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

Gene expression databases

Organism-specific databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

GO - Molecular functioni

Protein-protein interaction databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

Secondary structure

3D structure databases

Miscellaneous databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

Region

Motif

Compositional bias

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Zinc finger

Keywords - Domaini

Phylogenomic databases

Family and domain databases

Experimental Info

Natural variant

Alternative sequence

Sequence databases

Genome annotation databases

Keywords - Coding sequence diversityi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

Polymorphism and mutation databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Miscellaneous databases

Gene expression databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Documents

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Functionⁱ

Pathwayⁱ: protein sumoylation

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

Keywords - Cellular componentⁱ

Pathology & Biotechⁱ

PTM / Processingⁱ

Keywords - PTMⁱ

Expressionⁱ

Interactionⁱ

GO - Molecular functionⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Keywords - Domainⁱ

Keywords - Coding sequence diversityⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ

Keywords - Technical termⁱ