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Protein

Diacylglycerol O-acyltransferase 1

Gene

DGAT1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the terminal and only committed step in triacylglycerol synthesis by using diacylglycerol and fatty acyl CoA as substrates. In contrast to DGAT2 it is not essential for survival. May be involved in VLDL (very low density lipoprotein) assembly. In liver, plays a role in esterifying exogenous fatty acids to glycerol. Functions as the major acyl-CoA retinol acyltransferase (ARAT) in the skin, where it acts to maintain retinoid homeostasis and prevent retinoid toxicity leading to skin and hair disorders.2 Publications

Catalytic activityi

Acyl-CoA + 1,2-diacylglycerol = CoA + triacylglycerol.1 Publication
Acyl-CoA + retinol = CoA + retinyl ester.1 Publication

Activity regulationi

XP620 is a selective DGAT1 inhibitor.1 Publication

Kineticsi

  1. KM=25.9 µM for retinol1 Publication
  2. KM=13.9 µM for palmitoyl coenzyme A1 Publication

    Pathwayi: glycerolipid metabolism

    This protein is involved in the pathway glycerolipid metabolism, which is part of Lipid metabolism.
    View all proteins of this organism that are known to be involved in the pathway glycerolipid metabolism and in Lipid metabolism.

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Active sitei415By similarity1

    GO - Molecular functioni

    GO - Biological processi

    Keywordsi

    Molecular functionAcyltransferase, Transferase

    Enzyme and pathway databases

    BRENDAi2.3.1.20 2681
    ReactomeiR-HSA-1482883 Acyl chain remodeling of DAG and TAG
    R-HSA-6798695 Neutrophil degranulation
    R-HSA-75109 Triglyceride biosynthesis
    SIGNORiO75907
    UniPathwayi
    UPA00230

    Chemistry databases

    SwissLipidsiSLP:000000308

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Diacylglycerol O-acyltransferase 1 (EC:2.3.1.201 Publication)
    Alternative name(s):
    ACAT-related gene product 1
    Acyl-CoA retinol O-fatty-acyltransferase (EC:2.3.1.761 Publication)
    Short name:
    ARAT
    Short name:
    Retinol O-fatty-acyltransferase
    Diglyceride acyltransferase
    Gene namesi
    Name:DGAT1
    Synonyms:AGRP1, DGAT
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    Proteomesi
    • UP000005640 Componenti: Chromosome 8

    Organism-specific databases

    EuPathDBiHostDB:ENSG00000185000.9
    HGNCiHGNC:2843 DGAT1
    MIMi604900 gene
    neXtProtiNX_O75907

    Subcellular locationi

    Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

    Topology

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Topological domaini1 – 87CytoplasmicSequence analysisAdd BLAST87
    Transmembranei88 – 109HelicalSequence analysisAdd BLAST22
    Topological domaini110 – 220LumenalSequence analysisAdd BLAST111
    Transmembranei221 – 229HelicalSequence analysis9
    Topological domaini230CytoplasmicSequence analysis1
    Transmembranei231 – 239HelicalSequence analysis9
    Topological domaini240 – 488LumenalSequence analysisAdd BLAST249

    Keywords - Cellular componenti

    Endoplasmic reticulum, Membrane

    Pathology & Biotechi

    Involvement in diseasei

    Diarrhea 7 (DIAR7)1 Publication
    The disease is caused by mutations affecting the gene represented in this entry.
    Disease descriptionA life-threatening disease characterized by severe, intractable, watery diarrhea.
    See also OMIM:615863

    Organism-specific databases

    DisGeNETi8694
    MalaCardsiDGAT1
    MIMi615863 phenotype
    OpenTargetsiENSG00000185000
    Orphaneti329242 Congenital chronic diarrhea with protein-losing enteropathy
    PharmGKBiPA27303

    Chemistry databases

    ChEMBLiCHEMBL6009
    GuidetoPHARMACOLOGYi2821

    Polymorphism and mutation databases

    BioMutaiDGAT1

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00002076541 – 488Diacylglycerol O-acyltransferase 1Add BLAST488

    Amino acid modifications

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Modified residuei17PhosphoserineCombined sources1
    Modified residuei18PhosphoserineCombined sources1

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    EPDiO75907
    MaxQBiO75907
    PaxDbiO75907
    PeptideAtlasiO75907
    PRIDEiO75907
    ProteomicsDBi50257

    PTM databases

    iPTMnetiO75907
    PhosphoSitePlusiO75907

    Expressioni

    Gene expression databases

    BgeeiENSG00000185000 Expressed in 91 organ(s), highest expression level in small intestine Peyer's patch
    CleanExiHS_DGAT1
    ExpressionAtlasiO75907 baseline and differential
    GenevisibleiO75907 HS

    Organism-specific databases

    HPAiCAB032853

    Interactioni

    Subunit structurei

    Homodimer or homotetramer.By similarity

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    Q99IB82EBI-3906527,EBI-6927873From Hepatitis C virus genotype 2a (isolate JFH-1).

    Protein-protein interaction databases

    BioGridi114241, 14 interactors
    IntActiO75907, 5 interactors
    MINTiO75907
    STRINGi9606.ENSP00000332258

    Chemistry databases

    BindingDBiO75907

    Structurei

    3D structure databases

    ProteinModelPortaliO75907
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Regioni1 – 91Involved in homomerizationBy similarityAdd BLAST91

    Sequence similaritiesi

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiKOG0380 Eukaryota
    COG5056 LUCA
    GeneTreeiENSGT00530000063122
    HOGENOMiHOG000213917
    HOVERGENiHBG051341
    InParanoidiO75907
    KOiK11155
    OMAiYFWLAPT
    OrthoDBiEOG091G0GW2
    PhylomeDBiO75907
    TreeFamiTF314921

    Family and domain databases

    InterProiView protein in InterPro
    IPR027251 Diacylglycerol_acylTrfase1
    IPR004299 MBOAT_fam
    IPR014371 Oat_ACAT_DAG_ARE
    PANTHERiPTHR10408 PTHR10408, 1 hit
    PTHR10408:SF13 PTHR10408:SF13, 1 hit
    PfamiView protein in Pfam
    PF03062 MBOAT, 1 hit
    PIRSFiPIRSF000439 Oat_ACAT_DAG_ARE, 1 hit
    PIRSF500231 Oat_dag, 1 hit

    Sequence (1+)i

    Sequence statusi: Complete.

    This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All

    O75907-1 [UniParc]FASTAAdd to basket
    « Hide
            10         20         30         40         50
    MGDRGSSRRR RTGSRPSSHG GGGPAAAEEE VRDAAAGPDV GAAGDAPAPA
    60 70 80 90 100
    PNKDGDAGVG SGHWELRCHR LQDSLFSSDS GFSNYRGILN WCVVMLILSN
    110 120 130 140 150
    ARLFLENLIK YGILVDPIQV VSLFLKDPYS WPAPCLVIAA NVFAVAAFQV
    160 170 180 190 200
    EKRLAVGALT EQAGLLLHVA NLATILCFPA AVVLLVESIT PVGSLLALMA
    210 220 230 240 250
    HTILFLKLFS YRDVNSWCRR ARAKAASAGK KASSAAAPHT VSYPDNLTYR
    260 270 280 290 300
    DLYYFLFAPT LCYELNFPRS PRIRKRFLLR RILEMLFFTQ LQVGLIQQWM
    310 320 330 340 350
    VPTIQNSMKP FKDMDYSRII ERLLKLAVPN HLIWLIFFYW LFHSCLNAVA
    360 370 380 390 400
    ELMQFGDREF YRDWWNSESV TYFWQNWNIP VHKWCIRHFY KPMLRRGSSK
    410 420 430 440 450
    WMARTGVFLA SAFFHEYLVS VPLRMFRLWA FTGMMAQIPL AWFVGRFFQG
    460 470 480
    NYGNAAVWLS LIIGQPIAVL MYVHDYYVLN YEAPAAEA
    Length:488
    Mass (Da):55,278
    Last modified:July 25, 2003 - v2
    Checksum:i6574D5DBF15D6171
    GO

    Computationally mapped potential isoform sequencesi

    There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
    EntryEntry nameProtein names
    Gene namesLengthAnnotation
    A0A0A0MR74A0A0A0MR74_HUMAN
    Diacylglycerol O-acyltransferase 1
    DGAT1
    315Annotation score:

    Experimental Info

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Sequence conflicti129Y → H in AAC63997 (PubMed:9756920).Curated1

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AF059202 mRNA Translation: AAC63997.1
    AB057815 mRNA Translation: BAC66170.1
    CH471162 Genomic DNA Translation: EAW82127.1
    CH471162 Genomic DNA Translation: EAW82129.1
    BC015762 mRNA Translation: AAH15762.1
    BC023565 mRNA Translation: AAH23565.1
    BC150649 mRNA Translation: AAI50650.1
    CCDSiCCDS6420.1
    RefSeqiNP_036211.2, NM_012079.5
    UniGeneiHs.521954

    Genome annotation databases

    EnsembliENST00000528718; ENSP00000482264; ENSG00000185000
    ENST00000644790; ENSP00000495489; ENSG00000285482
    GeneIDi8694
    KEGGihsa:8694
    UCSCiuc003zbv.5 human

    Similar proteinsi

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AF059202 mRNA Translation: AAC63997.1
    AB057815 mRNA Translation: BAC66170.1
    CH471162 Genomic DNA Translation: EAW82127.1
    CH471162 Genomic DNA Translation: EAW82129.1
    BC015762 mRNA Translation: AAH15762.1
    BC023565 mRNA Translation: AAH23565.1
    BC150649 mRNA Translation: AAI50650.1
    CCDSiCCDS6420.1
    RefSeqiNP_036211.2, NM_012079.5
    UniGeneiHs.521954

    3D structure databases

    ProteinModelPortaliO75907
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    BioGridi114241, 14 interactors
    IntActiO75907, 5 interactors
    MINTiO75907
    STRINGi9606.ENSP00000332258

    Chemistry databases

    BindingDBiO75907
    ChEMBLiCHEMBL6009
    GuidetoPHARMACOLOGYi2821
    SwissLipidsiSLP:000000308

    PTM databases

    iPTMnetiO75907
    PhosphoSitePlusiO75907

    Polymorphism and mutation databases

    BioMutaiDGAT1

    Proteomic databases

    EPDiO75907
    MaxQBiO75907
    PaxDbiO75907
    PeptideAtlasiO75907
    PRIDEiO75907
    ProteomicsDBi50257

    Protocols and materials databases

    DNASUi8694
    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsembliENST00000528718; ENSP00000482264; ENSG00000185000
    ENST00000644790; ENSP00000495489; ENSG00000285482
    GeneIDi8694
    KEGGihsa:8694
    UCSCiuc003zbv.5 human

    Organism-specific databases

    CTDi8694
    DisGeNETi8694
    EuPathDBiHostDB:ENSG00000185000.9
    GeneCardsiDGAT1
    HGNCiHGNC:2843 DGAT1
    HPAiCAB032853
    MalaCardsiDGAT1
    MIMi604900 gene
    615863 phenotype
    neXtProtiNX_O75907
    OpenTargetsiENSG00000185000
    Orphaneti329242 Congenital chronic diarrhea with protein-losing enteropathy
    PharmGKBiPA27303
    GenAtlasiSearch...

    Phylogenomic databases

    eggNOGiKOG0380 Eukaryota
    COG5056 LUCA
    GeneTreeiENSGT00530000063122
    HOGENOMiHOG000213917
    HOVERGENiHBG051341
    InParanoidiO75907
    KOiK11155
    OMAiYFWLAPT
    OrthoDBiEOG091G0GW2
    PhylomeDBiO75907
    TreeFamiTF314921

    Enzyme and pathway databases

    UniPathwayi
    UPA00230

    BRENDAi2.3.1.20 2681
    ReactomeiR-HSA-1482883 Acyl chain remodeling of DAG and TAG
    R-HSA-6798695 Neutrophil degranulation
    R-HSA-75109 Triglyceride biosynthesis
    SIGNORiO75907

    Miscellaneous databases

    ChiTaRSiDGAT1 human
    GenomeRNAii8694
    PROiPR:O75907
    SOURCEiSearch...

    Gene expression databases

    BgeeiENSG00000185000 Expressed in 91 organ(s), highest expression level in small intestine Peyer's patch
    CleanExiHS_DGAT1
    ExpressionAtlasiO75907 baseline and differential
    GenevisibleiO75907 HS

    Family and domain databases

    InterProiView protein in InterPro
    IPR027251 Diacylglycerol_acylTrfase1
    IPR004299 MBOAT_fam
    IPR014371 Oat_ACAT_DAG_ARE
    PANTHERiPTHR10408 PTHR10408, 1 hit
    PTHR10408:SF13 PTHR10408:SF13, 1 hit
    PfamiView protein in Pfam
    PF03062 MBOAT, 1 hit
    PIRSFiPIRSF000439 Oat_ACAT_DAG_ARE, 1 hit
    PIRSF500231 Oat_dag, 1 hit
    ProtoNetiSearch...

    Entry informationi

    Entry nameiDGAT1_HUMAN
    AccessioniPrimary (citable) accession number: O75907
    Secondary accession number(s): B2RWQ2, D3DWL6, Q96BB8
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 18, 2001
    Last sequence update: July 25, 2003
    Last modified: September 12, 2018
    This is version 157 of the entry and version 2 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    3. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    4. Human chromosome 8
      Human chromosome 8: entries, gene names and cross-references to MIM
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