UniProtKB - O75818 (RPP40_HUMAN)
Ribonuclease P protein subunit p40
RPP40
Functioni
Component of ribonuclease P, a ribonucleoprotein complex that generates mature tRNA molecules by cleaving their 5'-ends (PubMed:9630247, PubMed:30454648).
Also a component of the MRP ribonuclease complex, which cleaves pre-rRNA sequences (PubMed:28115465).
3 PublicationsGO - Molecular functioni
- ribonuclease P activity Source: ProtInc
- ribonuclease P RNA binding Source: UniProtKB
GO - Biological processi
- endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) Source: GO_Central
- endonucleolytic cleavage involved in tRNA processing Source: GO_Central
- tRNA 5'-leader removal Source: UniProtKB
Keywordsi
Molecular function | RNA-binding |
Biological process | rRNA processing, tRNA processing |
Enzyme and pathway databases
BRENDAi | 3.1.26.5, 2681 |
PathwayCommonsi | O75818 |
Reactomei | R-HSA-6784531, tRNA processing in the nucleus R-HSA-6791226, Major pathway of rRNA processing in the nucleolus and cytosol |
SignaLinki | O75818 |
Names & Taxonomyi
Protein namesi | Recommended name: Ribonuclease P protein subunit p40Short name: RNaseP protein p40 Alternative name(s): RNase P subunit 1 |
Gene namesi | Name:RPP40 Synonyms:RNASEP1 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:20992, RPP40 |
MIMi | 606117, gene |
neXtProti | NX_O75818 |
VEuPathDBi | HostDB:ENSG00000124787 |
Subcellular locationi
Nucleus
- nucleolus Curated
Nucleus
- nucleolar ribonuclease P complex Source: ProtInc
- nucleoplasm Source: Reactome
- nucleus Source: ProtInc
Other locations
- multimeric ribonuclease P complex Source: UniProtKB
- ribonuclease MRP complex Source: GO_Central
Keywords - Cellular componenti
NucleusPathology & Biotechi
Organism-specific databases
OpenTargetsi | ENSG00000124787 |
PharmGKBi | PA134911809 |
Miscellaneous databases
Pharosi | O75818, Tbio |
Genetic variation databases
BioMutai | RPP40 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000097434 | 1 – 363 | Ribonuclease P protein subunit p40Add BLAST | 363 |
Proteomic databases
EPDi | O75818 |
jPOSTi | O75818 |
MassIVEi | O75818 |
MaxQBi | O75818 |
PaxDbi | O75818 |
PeptideAtlasi | O75818 |
PRIDEi | O75818 |
ProteomicsDBi | 50208 [O75818-1] 50209 [O75818-2] |
PTM databases
iPTMneti | O75818 |
PhosphoSitePlusi | O75818 |
Expressioni
Gene expression databases
Bgeei | ENSG00000124787, Expressed in left adrenal gland and 208 other tissues |
ExpressionAtlasi | O75818, baseline and differential |
Genevisiblei | O75818, HS |
Organism-specific databases
HPAi | ENSG00000124787, Low tissue specificity |
Interactioni
Subunit structurei
Component of nuclear RNase P and RNase MRP ribonucleoproteins (PubMed:9630247, PubMed:16723659, PubMed:28115465, PubMed:30454648). RNase P consists of a catalytic RNA moiety and about 10 protein subunits; POP1, POP4, POP5, POP7, RPP14, RPP21, RPP25, RPP30, RPP38 and RPP40 (PubMed:9630247, PubMed:16723659, PubMed:30454648). Within the RNase P complex, POP1, POP7 and RPP25 form the 'finger' subcomplex, POP5, RPP14, RPP40 and homodimeric RPP30 form the 'palm' subcomplex, and RPP21, POP4 and RPP38 form the 'wrist' subcomplex. All subunits of the RNase P complex interact with the catalytic RNA (PubMed:30454648). Several subunits of RNase P are also part of the RNase MRP complex. RNase MRP consists of a catalytic RNA moiety and about 8 protein subunits; POP1, POP7, RPP25, RPP30, RPP38, RPP40 and possibly also POP4 and POP5 (PubMed:16723659, PubMed:28115465).
4 PublicationsBinary interactionsi
Isoform 2 [O75818-2]
With | #Exp. | IntAct |
---|---|---|
MEOX2 [Q6FHY5] | 3 | EBI-12401132,EBI-16439278 |
Protein-protein interaction databases
BioGRIDi | 116013, 37 interactors |
CORUMi | O75818 |
IntActi | O75818, 16 interactors |
STRINGi | 9606.ENSP00000369391 |
Miscellaneous databases
RNActi | O75818, protein |
Structurei
Secondary structure
3D structure databases
SMRi | O75818 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Phylogenomic databases
eggNOGi | ENOG502QSAV, Eukaryota |
GeneTreei | ENSGT00390000014167 |
InParanoidi | O75818 |
OMAi | LQWETGF |
OrthoDBi | 1273379at2759 |
PhylomeDBi | O75818 |
TreeFami | TF330967 |
Family and domain databases
InterProi | View protein in InterPro IPR013893, RNase_P_Rpp40 |
PANTHERi | PTHR15396, PTHR15396, 1 hit |
Pfami | View protein in Pfam PF08584, Ribonuc_P_40, 1 hit |
s (2+)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 3 potential isoforms that are computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MATLRRLREA PRHLLVCEKS NFGNHKSRHR HLVQTHYYNY RVSFLIPECG
60 70 80 90 100
ILSEELKNLV MNTGPYYFVK NLPLHELITP EFISTFIKKG SCYALTYNTH
110 120 130 140 150
IDEDNTVALL PNGKLILSLD KDTYEETGLQ GHPSQFSGRK IMKFIVSIDL
160 170 180 190 200
MELSLNLDSK KYERISWSFK EKKPLKFDFL LAWHKTGSEE STMMSYFSKY
210 220 230 240 250
QIQEHQPKVA LSTLRDLQCP VLQSSELEGT PEVSCRALEL FDWLGAVFSN
260 270 280 290 300
VDLNNEPNNF ISTYCCPEPS TVVAKAYLCT ITGFILPEKI CLLLEHLCHY
310 320 330 340 350
FDEPKLAPWV TLSVQGFADS PVSWEKNEHG FRKGGEHLYN FVIFNNQDYW
360
LQMAVGANDH CPP
Computationally mapped potential isoform sequencesi
There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketA0A087X1N3 | A0A087X1N3_HUMAN | Ribonuclease P 40kDa subunit, isofo... | RPP40 hCG_1783141 | 321 | Annotation score: | ||
F8WC38 | F8WC38_HUMAN | Ribonuclease P protein subunit p40 | RPP40 | 113 | Annotation score: | ||
X6RLL4 | X6RLL4_HUMAN | Ribonuclease P protein subunit p40 | RPP40 | 303 | Annotation score: |
Sequence cautioni
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 255 | Missing in AAC24114 (PubMed:9630247).Curated | 1 |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_055405 | 314 | V → I. Corresponds to variant dbSNP:rs12332997Ensembl. | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_037346 | 90 – 112 | Missing in isoform 2. 1 PublicationAdd BLAST | 23 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AL359643 Genomic DNA No translation available. BC017871 mRNA Translation: AAH17871.1 Sequence problems. U94317 Genomic DNA Translation: AAC24114.1 Different initiation. |
CCDSi | CCDS34333.1 [O75818-1] CCDS69040.1 [O75818-2] |
RefSeqi | NP_001273061.1, NM_001286132.1 [O75818-2] NP_001273062.1, NM_001286133.1 NP_006629.2, NM_006638.3 [O75818-1] |
Genome annotation databases
Ensembli | ENST00000319533; ENSP00000317998; ENSG00000124787 [O75818-2] ENST00000380051; ENSP00000369391; ENSG00000124787 |
GeneIDi | 10799 |
KEGGi | hsa:10799 |
MANE-Selecti | ENST00000380051.7; ENSP00000369391.2; NM_006638.4; NP_006629.2 |
UCSCi | uc003mwl.5, human [O75818-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AL359643 Genomic DNA No translation available. BC017871 mRNA Translation: AAH17871.1 Sequence problems. U94317 Genomic DNA Translation: AAC24114.1 Different initiation. |
CCDSi | CCDS34333.1 [O75818-1] CCDS69040.1 [O75818-2] |
RefSeqi | NP_001273061.1, NM_001286132.1 [O75818-2] NP_001273062.1, NM_001286133.1 NP_006629.2, NM_006638.3 [O75818-1] |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
6AHR | electron microscopy | 3.92 | L | 1-363 | [»] | |
6AHU | electron microscopy | 3.66 | L | 1-363 | [»] | |
6AHV | X-ray | 2.60 | A | 1-363 | [»] | |
SMRi | O75818 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 116013, 37 interactors |
CORUMi | O75818 |
IntActi | O75818, 16 interactors |
STRINGi | 9606.ENSP00000369391 |
PTM databases
iPTMneti | O75818 |
PhosphoSitePlusi | O75818 |
Genetic variation databases
BioMutai | RPP40 |
Proteomic databases
EPDi | O75818 |
jPOSTi | O75818 |
MassIVEi | O75818 |
MaxQBi | O75818 |
PaxDbi | O75818 |
PeptideAtlasi | O75818 |
PRIDEi | O75818 |
ProteomicsDBi | 50208 [O75818-1] 50209 [O75818-2] |
Protocols and materials databases
Antibodypediai | 24532, 76 antibodies from 20 providers |
DNASUi | 10799 |
Genome annotation databases
Ensembli | ENST00000319533; ENSP00000317998; ENSG00000124787 [O75818-2] ENST00000380051; ENSP00000369391; ENSG00000124787 |
GeneIDi | 10799 |
KEGGi | hsa:10799 |
MANE-Selecti | ENST00000380051.7; ENSP00000369391.2; NM_006638.4; NP_006629.2 |
UCSCi | uc003mwl.5, human [O75818-1] |
Organism-specific databases
CTDi | 10799 |
GeneCardsi | RPP40 |
HGNCi | HGNC:20992, RPP40 |
HPAi | ENSG00000124787, Low tissue specificity |
MIMi | 606117, gene |
neXtProti | NX_O75818 |
OpenTargetsi | ENSG00000124787 |
PharmGKBi | PA134911809 |
VEuPathDBi | HostDB:ENSG00000124787 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | ENOG502QSAV, Eukaryota |
GeneTreei | ENSGT00390000014167 |
InParanoidi | O75818 |
OMAi | LQWETGF |
OrthoDBi | 1273379at2759 |
PhylomeDBi | O75818 |
TreeFami | TF330967 |
Enzyme and pathway databases
BRENDAi | 3.1.26.5, 2681 |
PathwayCommonsi | O75818 |
Reactomei | R-HSA-6784531, tRNA processing in the nucleus R-HSA-6791226, Major pathway of rRNA processing in the nucleolus and cytosol |
SignaLinki | O75818 |
Miscellaneous databases
BioGRID-ORCSi | 10799, 606 hits in 1053 CRISPR screens |
ChiTaRSi | RPP40, human |
GeneWikii | RPP40 |
GenomeRNAii | 10799 |
Pharosi | O75818, Tbio |
PROi | PR:O75818 |
RNActi | O75818, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000124787, Expressed in left adrenal gland and 208 other tissues |
ExpressionAtlasi | O75818, baseline and differential |
Genevisiblei | O75818, HS |
Family and domain databases
InterProi | View protein in InterPro IPR013893, RNase_P_Rpp40 |
PANTHERi | PTHR15396, PTHR15396, 1 hit |
Pfami | View protein in Pfam PF08584, Ribonuc_P_40, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | RPP40_HUMAN | |
Accessioni | O75818Primary (citable) accession number: O75818 Secondary accession number(s): Q5VX97, Q8WVK8 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 21, 2001 |
Last sequence update: | May 26, 2009 | |
Last modified: | February 23, 2022 | |
This is version 166 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- Human chromosome 6
Human chromosome 6: entries, gene names and cross-references to MIM - Human entries with genetic variants
List of human entries with genetic variants - Human variants curated from literature reports
Index of human variants curated from literature reports - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references