UniProtKB - O75815 (BCAR3_HUMAN)
Breast cancer anti-estrogen resistance protein 3
BCAR3
Functioni
Miscellaneous
Caution
GO - Molecular functioni
- guanyl-nucleotide exchange factor activity Source: UniProtKB-KW
- kinase binding Source: UniProtKB
- phosphotyrosine residue binding Source: UniProtKB
GO - Biological processi
- endothelin receptor signaling pathway Source: UniProtKB
- insulin receptor signaling pathway Source: UniProtKB
- lens morphogenesis in camera-type eye Source: Ensembl
- positive regulation of DNA replication Source: UniProtKB
- positive regulation of GTPase activity Source: UniProtKB
- positive regulation of MAPK cascade Source: UniProtKB
- positive regulation of peptidyl-serine phosphorylation Source: GO_Central
- response to drug Source: ProtInc
- signal transduction Source: ProtInc
- small GTPase mediated signal transduction Source: InterPro
Keywordsi
Molecular function | Guanine-nucleotide releasing factor |
Enzyme and pathway databases
PathwayCommonsi | O75815 |
SignaLinki | O75815 |
Names & Taxonomyi
Protein namesi | Recommended name: Breast cancer anti-estrogen resistance protein 3Alternative name(s): Novel SH2-containing protein 2 SH2 domain-containing protein 3B |
Gene namesi | Name:BCAR3 Synonyms:NSP2, SH2D3B ORF Names:UNQ271/PRO308 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
EuPathDBi | HostDB:ENSG00000137936.16 |
HGNCi | HGNC:973, BCAR3 |
MIMi | 604704, gene |
neXtProti | NX_O75815 |
Subcellular locationi
Other locations
- Cytoplasm By similarity
- focal adhesion By similarity
Note: Localization to focal adhesions depends on interaction with PTPRA.By similarity
Other locations
- cytoplasm Source: UniProtKB-SubCell
- focal adhesion Source: UniProtKB-SubCell
Keywords - Cellular componenti
Cell junction, CytoplasmPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 177 | R → K: Abolishes localization to focal adhesions. Reduces interaction with PTPRA. 1 Publication | 1 | |
Mutagenesisi | 744 | L → E: Weakens interaction with BCAR1. 1 Publication | 1 | |
Mutagenesisi | 748 | R → A: Abolishes interaction with BCAR1 and SRC. 2 Publications | 1 |
Organism-specific databases
DisGeNETi | 8412 |
OpenTargetsi | ENSG00000137936 |
PharmGKBi | PA25283 |
Miscellaneous databases
Pharosi | O75815, Tbio |
Polymorphism and mutation databases
BioMutai | BCAR3 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | RemovedCombined sources | |||
ChainiPRO_0000230284 | 2 – 825 | Breast cancer anti-estrogen resistance protein 3Add BLAST | 824 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 2 | N-acetylalanineCombined sources | 1 | |
Modified residuei | 32 | PhosphoserineCombined sources | 1 | |
Modified residuei | 78 | PhosphoserineCombined sources | 1 | |
Modified residuei | 83 | PhosphoserineCombined sources | 1 | |
Modified residuei | 182 | PhosphoserineCombined sources | 1 | |
Modified residuei | 290 | PhosphoserineCombined sources | 1 | |
Modified residuei | 334 | N6-methyllysineCombined sources | 1 | |
Modified residuei | 358 | PhosphoserineCombined sources | 1 | |
Modified residuei | 363 | PhosphoserineCombined sources | 1 | |
Modified residuei | 375 | PhosphoserineCombined sources | 1 | |
Modified residuei | 442 | Omega-N-methylarginineCombined sources | 1 | |
Modified residuei | 471 | PhosphoserineBy similarity | 1 |
Post-translational modificationi
Keywords - PTMi
Acetylation, Methylation, PhosphoproteinProteomic databases
EPDi | O75815 |
jPOSTi | O75815 |
MassIVEi | O75815 |
MaxQBi | O75815 |
PaxDbi | O75815 |
PeptideAtlasi | O75815 |
PRIDEi | O75815 |
ProteomicsDBi | 50205 [O75815-1] 50206 [O75815-2] 65066 |
PTM databases
iPTMneti | O75815 |
PhosphoSitePlusi | O75815 |
Expressioni
Tissue specificityi
Gene expression databases
Bgeei | ENSG00000137936, Expressed in chorionic villus and 234 other tissues |
ExpressionAtlasi | O75815, baseline and differential |
Genevisiblei | O75815, HS |
Organism-specific databases
HPAi | ENSG00000137936, Tissue enhanced (placenta) |
Interactioni
Subunit structurei
Part of a complex comprised of PTPRA, BCAR1, BCAR3 (via SH2 domain) and SRC; the formation of the complex is dependent on integrin mediated-tyrosine phosphorylation of PTPRA (PubMed:22801373). Within the complex, interacts (via SH2 domain) with PTPRA (when phosphorylated on 'Tyr-798') (PubMed:22801373).
Interacts (via Ras-GEF domain) with BCAR1 (PubMed:18722344, PubMed:19086031, PubMed:22081014).
Interacts (via Ras-GEF domain) with NEDD9 (By similarity).
Interacts with PTK2/FAK1 (By similarity).
Interacts with PTPN1.
Interacts (via SH2 domain) with EGFR (when tyrosine-phosphorylated) (PubMed:18722344).
By similarity4 PublicationsBinary interactionsi
Hide detailsO75815
Isoform 1 [O75815-1]
With | #Exp. | IntAct |
---|---|---|
BCAR1 [P56945] | 3 | EBI-15953103,EBI-702093 |
GO - Molecular functioni
- guanyl-nucleotide exchange factor activity Source: UniProtKB-KW
- kinase binding Source: UniProtKB
- phosphotyrosine residue binding Source: UniProtKB
Protein-protein interaction databases
BioGRIDi | 114000, 44 interactors |
DIPi | DIP-33857N |
IntActi | O75815, 41 interactors |
MINTi | O75815 |
STRINGi | 9606.ENSP00000359264 |
Miscellaneous databases
RNActi | O75815, protein |
Structurei
Secondary structure
3D structure databases
SMRi | O75815 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 154 – 253 | SH2PROSITE-ProRule annotationAdd BLAST | 100 | |
Domaini | 548 – 818 | Ras-GEFPROSITE-ProRule annotationAdd BLAST | 271 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 744 – 748 | Mediates the interaction with BCAR1/p130CAS1 Publication | 5 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 479 – 482 | Poly-Asp | 4 |
Domaini
Keywords - Domaini
SH2 domainPhylogenomic databases
eggNOGi | ENOG502QPX3, Eukaryota |
GeneTreei | ENSGT00940000154130 |
HOGENOMi | CLU_015281_0_0_1 |
InParanoidi | O75815 |
OMAi | CSAFHAF |
OrthoDBi | 138275at2759 |
PhylomeDBi | O75815 |
TreeFami | TF323756 |
Family and domain databases
CDDi | cd00155, RasGEF, 1 hit cd10337, SH2_BCAR3, 1 hit |
Gene3Di | 1.10.840.10, 1 hit 3.30.505.10, 1 hit |
InterProi | View protein in InterPro IPR028849, BCAR3 IPR023578, Ras_GEF_dom_sf IPR001895, RASGEF_cat_dom IPR036964, RASGEF_cat_dom_sf IPR000980, SH2 IPR036860, SH2_dom_sf IPR044102, SH2_SHEP1/BCAR3/NSP1 |
PANTHERi | PTHR14247:SF10, PTHR14247:SF10, 1 hit |
Pfami | View protein in Pfam PF00617, RasGEF, 1 hit PF00017, SH2, 1 hit |
SMARTi | View protein in SMART SM00147, RasGEF, 1 hit SM00252, SH2, 1 hit |
SUPFAMi | SSF48366, SSF48366, 1 hit SSF55550, SSF55550, 1 hit |
PROSITEi | View protein in PROSITE PS50009, RASGEF_CAT, 1 hit PS50001, SH2, 1 hit |
s (3)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry describes 3 produced by isoformsialternative splicing. AlignAdd to basketThis isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MAAGKFASLP RNMPVNHQFP LASSMDLLSS RSPLAEHRPD AYQDVSIHGT
60 70 80 90 100
LPRKKKGPPP IRSCDDFSHM GTLPHSKSPR QNSPVTQDGI QESPWQDRHG
110 120 130 140 150
ETFTFRDPHL LDPTVEYVKF SKERHIMDRT PEKLKKELEE ELLLSSEDLR
160 170 180 190 200
SHAWYHGRIP RQVSENLVQR DGDFLVRDSL SSPGNFVLTC QWKNLAQHFK
210 220 230 240 250
INRTVLRLSE AYSRVQYQFE MESFDSIPGL VRCYVGNRRP ISQQSGAIIF
260 270 280 290 300
QPINRTVPLR CLEEHYGTSP GQAREGSLTK GRPDVAKRLS LTMGGVQARE
310 320 330 340 350
QNLPRGNLLR NKEKSGSQPA CLDHMQDRRA LSLKAHQSES YLPIGCKLPP
360 370 380 390 400
QSSGVDTSPC PNSPVFRTGS EPALSPAVVR RVSSDARAGE ALRGSDSQLC
410 420 430 440 450
PKPPPKPCKV PFLKVPSSPS AWLNSEANYC ELNPAFATGC GRGAKLPSCA
460 470 480 490 500
QGSHTELLTA KQNEAPGPRN SGVNYLILDD DDRERPWEPA AAQMEKGQWD
510 520 530 540 550
KGEFVTPLLE TVSSFRPNEF ESKFLPPENK PLETAMLKRA KELFTNNDPK
560 570 580 590 600
VIAQHVLSMD CRVARILGVS EEMRRNMGVS SGLELITLPH GHQLRLDIIE
610 620 630 640 650
RHNTMAIGIA VDILGCTGTL EDRAATLSKI IQVAVELKDS MGDLYSFSAL
660 670 680 690 700
MKALEMPQIT RLEKTWTALR HQYTQTAILY EKQLKPFSKL LHEGRESTCV
710 720 730 740 750
PPNNVSVPLL MPLVTLMERQ AVTFEGTDMW EKNDQSCEIM LNHLATARFM
760 770 780 790 800
AEAADSYRMN AERILAGFQP DEEMNEICKT EFQMRLLWGS KGAQVNQTER
810 820
YEKFNQILTA LSRKLEPPPV KQAEL
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 519 | E → G in AL833121 (PubMed:17974005).Curated | 1 | |
Sequence conflicti | 543 | L → P in AL833121 (PubMed:17974005).Curated | 1 |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_050689 | 464 | E → G. Corresponds to variant dbSNP:rs12062278Ensembl. | 1 | |
Natural variantiVAR_050690 | 593 | Q → H. Corresponds to variant dbSNP:rs17110107Ensembl. | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_017814 | 1 – 324 | Missing in isoform 2. 1 PublicationAdd BLAST | 324 | |
Alternative sequenceiVSP_046716 | 1 – 91 | Missing in isoform 3. 1 PublicationAdd BLAST | 91 | |
Alternative sequenceiVSP_046717 | 92 – 117 | ESPWQ…PTVEY → MPKECSAFHALSAALCCFYH RKSFIG in isoform 3. 1 PublicationAdd BLAST | 26 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U92715 mRNA Translation: AAC39777.1 AF124250 mRNA Translation: AAD28245.1 AY358996 mRNA Translation: AAQ89355.1 AL833121 mRNA No translation available. AL049796 Genomic DNA No translation available. AL109613 Genomic DNA No translation available. AL359820 Genomic DNA No translation available. AL512488 Genomic DNA No translation available. CH471097 Genomic DNA Translation: EAW73065.1 CH471097 Genomic DNA Translation: EAW73066.1 CH471097 Genomic DNA Translation: EAW73068.1 BC039895 mRNA Translation: AAH39895.1 |
CCDSi | CCDS58010.1 [O75815-3] CCDS745.1 [O75815-1] CCDS76181.1 [O75815-2] |
RefSeqi | NP_001248337.1, NM_001261408.1 [O75815-1] NP_001248338.1, NM_001261409.1 [O75815-1] NP_001248339.1, NM_001261410.1 [O75815-3] NP_001295180.1, NM_001308251.1 [O75815-2] NP_003558.1, NM_003567.3 [O75815-1] XP_016857969.1, XM_017002480.1 [O75815-1] |
Genome annotation databases
Ensembli | ENST00000260502; ENSP00000260502; ENSG00000137936 [O75815-1] ENST00000370243; ENSP00000359263; ENSG00000137936 [O75815-1] ENST00000370244; ENSP00000359264; ENSG00000137936 [O75815-1] ENST00000370247; ENSP00000359267; ENSG00000137936 [O75815-3] ENST00000539242; ENSP00000441343; ENSG00000137936 [O75815-2] |
GeneIDi | 8412 |
KEGGi | hsa:8412 |
UCSCi | uc001dpx.6, human [O75815-1] |
Keywords - Coding sequence diversityi
Alternative splicing, PolymorphismSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U92715 mRNA Translation: AAC39777.1 AF124250 mRNA Translation: AAD28245.1 AY358996 mRNA Translation: AAQ89355.1 AL833121 mRNA No translation available. AL049796 Genomic DNA No translation available. AL109613 Genomic DNA No translation available. AL359820 Genomic DNA No translation available. AL512488 Genomic DNA No translation available. CH471097 Genomic DNA Translation: EAW73065.1 CH471097 Genomic DNA Translation: EAW73066.1 CH471097 Genomic DNA Translation: EAW73068.1 BC039895 mRNA Translation: AAH39895.1 |
CCDSi | CCDS58010.1 [O75815-3] CCDS745.1 [O75815-1] CCDS76181.1 [O75815-2] |
RefSeqi | NP_001248337.1, NM_001261408.1 [O75815-1] NP_001248338.1, NM_001261409.1 [O75815-1] NP_001248339.1, NM_001261410.1 [O75815-3] NP_001295180.1, NM_001308251.1 [O75815-2] NP_003558.1, NM_003567.3 [O75815-1] XP_016857969.1, XM_017002480.1 [O75815-1] |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
3T6A | X-ray | 2.40 | A/B/C/D | 502-825 | [»] | |
SMRi | O75815 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 114000, 44 interactors |
DIPi | DIP-33857N |
IntActi | O75815, 41 interactors |
MINTi | O75815 |
STRINGi | 9606.ENSP00000359264 |
PTM databases
iPTMneti | O75815 |
PhosphoSitePlusi | O75815 |
Polymorphism and mutation databases
BioMutai | BCAR3 |
Proteomic databases
EPDi | O75815 |
jPOSTi | O75815 |
MassIVEi | O75815 |
MaxQBi | O75815 |
PaxDbi | O75815 |
PeptideAtlasi | O75815 |
PRIDEi | O75815 |
ProteomicsDBi | 50205 [O75815-1] 50206 [O75815-2] 65066 |
Protocols and materials databases
Antibodypediai | 2973, 153 antibodies |
DNASUi | 8412 |
Genome annotation databases
Ensembli | ENST00000260502; ENSP00000260502; ENSG00000137936 [O75815-1] ENST00000370243; ENSP00000359263; ENSG00000137936 [O75815-1] ENST00000370244; ENSP00000359264; ENSG00000137936 [O75815-1] ENST00000370247; ENSP00000359267; ENSG00000137936 [O75815-3] ENST00000539242; ENSP00000441343; ENSG00000137936 [O75815-2] |
GeneIDi | 8412 |
KEGGi | hsa:8412 |
UCSCi | uc001dpx.6, human [O75815-1] |
Organism-specific databases
CTDi | 8412 |
DisGeNETi | 8412 |
EuPathDBi | HostDB:ENSG00000137936.16 |
GeneCardsi | BCAR3 |
HGNCi | HGNC:973, BCAR3 |
HPAi | ENSG00000137936, Tissue enhanced (placenta) |
MIMi | 604704, gene |
neXtProti | NX_O75815 |
OpenTargetsi | ENSG00000137936 |
PharmGKBi | PA25283 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | ENOG502QPX3, Eukaryota |
GeneTreei | ENSGT00940000154130 |
HOGENOMi | CLU_015281_0_0_1 |
InParanoidi | O75815 |
OMAi | CSAFHAF |
OrthoDBi | 138275at2759 |
PhylomeDBi | O75815 |
TreeFami | TF323756 |
Enzyme and pathway databases
PathwayCommonsi | O75815 |
SignaLinki | O75815 |
Miscellaneous databases
BioGRID-ORCSi | 8412, 21 hits in 845 CRISPR screens |
ChiTaRSi | BCAR3, human |
GeneWikii | BCAR3 |
GenomeRNAii | 8412 |
Pharosi | O75815, Tbio |
PROi | PR:O75815 |
RNActi | O75815, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000137936, Expressed in chorionic villus and 234 other tissues |
ExpressionAtlasi | O75815, baseline and differential |
Genevisiblei | O75815, HS |
Family and domain databases
CDDi | cd00155, RasGEF, 1 hit cd10337, SH2_BCAR3, 1 hit |
Gene3Di | 1.10.840.10, 1 hit 3.30.505.10, 1 hit |
InterProi | View protein in InterPro IPR028849, BCAR3 IPR023578, Ras_GEF_dom_sf IPR001895, RASGEF_cat_dom IPR036964, RASGEF_cat_dom_sf IPR000980, SH2 IPR036860, SH2_dom_sf IPR044102, SH2_SHEP1/BCAR3/NSP1 |
PANTHERi | PTHR14247:SF10, PTHR14247:SF10, 1 hit |
Pfami | View protein in Pfam PF00617, RasGEF, 1 hit PF00017, SH2, 1 hit |
SMARTi | View protein in SMART SM00147, RasGEF, 1 hit SM00252, SH2, 1 hit |
SUPFAMi | SSF48366, SSF48366, 1 hit SSF55550, SSF55550, 1 hit |
PROSITEi | View protein in PROSITE PS50009, RASGEF_CAT, 1 hit PS50001, SH2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | BCAR3_HUMAN | |
Accessioni | O75815Primary (citable) accession number: O75815 Secondary accession number(s): D3DT43 Q9BR50 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 4, 2006 |
Last sequence update: | November 1, 1998 | |
Last modified: | December 2, 2020 | |
This is version 171 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Human polymorphisms and disease mutations
Index of human polymorphisms and disease mutations - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - Human chromosome 1
Human chromosome 1: entries, gene names and cross-references to MIM - Human entries with polymorphisms or disease mutations
List of human entries with polymorphisms or disease mutations