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Protein

Breast cancer anti-estrogen resistance protein 3

Gene

BCAR3

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

May act as an adapter protein and couple activated growth factor receptors to a signaling pathway that regulates the proliferation in breast cancer cells. When overexpressed, it confers anti-estrogen resistance in breast cancer cell lines. May also be regulated by cellular adhesion to extracellular matrix proteins.1 Publication

GO - Molecular functioni

GO - Biological processi

  • lens morphogenesis in camera-type eye Source: Ensembl
  • positive regulation of peptidyl-serine phosphorylation Source: GO_Central
  • response to drug Source: ProtInc
  • signal transduction Source: ProtInc
  • small GTPase mediated signal transduction Source: InterPro

Keywordsi

Molecular functionGuanine-nucleotide releasing factor

Enzyme and pathway databases

SignaLinkiO75815

Names & Taxonomyi

Protein namesi
Recommended name:
Breast cancer anti-estrogen resistance protein 3
Alternative name(s):
Novel SH2-containing protein 2
SH2 domain-containing protein 3B
Gene namesi
Name:BCAR3
Synonyms:NSP2, SH2D3B
ORF Names:UNQ271/PRO308
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 1

Organism-specific databases

EuPathDBiHostDB:ENSG00000137936.16
HGNCiHGNC:973 BCAR3
MIMi604704 gene
neXtProtiNX_O75815

Subcellular locationi

Pathology & Biotechi

Organism-specific databases

DisGeNETi8412
OpenTargetsiENSG00000137936
PharmGKBiPA25283

Polymorphism and mutation databases

BioMutaiBCAR3

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources
ChainiPRO_00002302842 – 825Breast cancer anti-estrogen resistance protein 3Add BLAST824

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineCombined sources1
Modified residuei32PhosphoserineCombined sources1
Modified residuei78PhosphoserineCombined sources1
Modified residuei83PhosphoserineCombined sources1
Modified residuei182PhosphoserineCombined sources1
Modified residuei290PhosphoserineCombined sources1
Modified residuei334N6-methyllysineCombined sources1
Modified residuei358PhosphoserineCombined sources1
Modified residuei363PhosphoserineCombined sources1
Modified residuei375PhosphoserineCombined sources1
Modified residuei442Omega-N-methylarginineCombined sources1
Modified residuei471PhosphoserineBy similarity1

Keywords - PTMi

Acetylation, Methylation, Phosphoprotein

Proteomic databases

EPDiO75815
MaxQBiO75815
PaxDbiO75815
PeptideAtlasiO75815
PRIDEiO75815
ProteomicsDBi50205
50206 [O75815-2]

PTM databases

iPTMnetiO75815
PhosphoSitePlusiO75815

Expressioni

Tissue specificityi

Ubiquitously expressed. Found in several cancer cell lines, but not in nonmalignant breast tissue.2 Publications

Gene expression databases

BgeeiENSG00000137936 Expressed in 222 organ(s), highest expression level in chorionic villus
CleanExiHS_BCAR3
GenevisibleiO75815 HS

Organism-specific databases

HPAiHPA014858

Interactioni

Subunit structurei

Interacts with BCAR1, NEDD9, PTK2/FAK1 and PTPN1.By similarity

Binary interactionsi

GO - Molecular functioni

Protein-protein interaction databases

BioGridi114000, 38 interactors
DIPiDIP-33857N
IntActiO75815, 38 interactors
MINTiO75815
STRINGi9606.ENSP00000260502

Structurei

Secondary structure

1825
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliO75815
SMRiO75815
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini154 – 253SH2PROSITE-ProRule annotationAdd BLAST100
Domaini548 – 818Ras-GEFPROSITE-ProRule annotationAdd BLAST271

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi479 – 482Poly-Asp4

Keywords - Domaini

SH2 domain

Phylogenomic databases

eggNOGiENOG410IFQG Eukaryota
ENOG410XTJR LUCA
GeneTreeiENSGT00390000008976
HOGENOMiHOG000231595
HOVERGENiHBG053174
InParanoidiO75815
OMAiMDCKVAR
OrthoDBiEOG091G01KG
PhylomeDBiO75815
TreeFamiTF323756

Family and domain databases

CDDicd00155 RasGEF, 1 hit
Gene3Di1.10.840.10, 1 hit
3.30.505.10, 1 hit
InterProiView protein in InterPro
IPR028849 BCAR3
IPR023578 Ras_GEF_dom_sf
IPR001895 RASGEF_cat_dom
IPR036964 RASGEF_cat_dom_sf
IPR000980 SH2
IPR036860 SH2_dom_sf
PANTHERiPTHR14247:SF10 PTHR14247:SF10, 1 hit
PfamiView protein in Pfam
PF00617 RasGEF, 1 hit
PF00017 SH2, 1 hit
SMARTiView protein in SMART
SM00147 RasGEF, 1 hit
SM00252 SH2, 1 hit
SUPFAMiSSF48366 SSF48366, 1 hit
SSF55550 SSF55550, 1 hit
PROSITEiView protein in PROSITE
PS50009 RASGEF_CAT, 1 hit
PS50001 SH2, 1 hit

Sequences (3)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket
Isoform 1 (identifier: O75815-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MAAGKFASLP RNMPVNHQFP LASSMDLLSS RSPLAEHRPD AYQDVSIHGT
60 70 80 90 100
LPRKKKGPPP IRSCDDFSHM GTLPHSKSPR QNSPVTQDGI QESPWQDRHG
110 120 130 140 150
ETFTFRDPHL LDPTVEYVKF SKERHIMDRT PEKLKKELEE ELLLSSEDLR
160 170 180 190 200
SHAWYHGRIP RQVSENLVQR DGDFLVRDSL SSPGNFVLTC QWKNLAQHFK
210 220 230 240 250
INRTVLRLSE AYSRVQYQFE MESFDSIPGL VRCYVGNRRP ISQQSGAIIF
260 270 280 290 300
QPINRTVPLR CLEEHYGTSP GQAREGSLTK GRPDVAKRLS LTMGGVQARE
310 320 330 340 350
QNLPRGNLLR NKEKSGSQPA CLDHMQDRRA LSLKAHQSES YLPIGCKLPP
360 370 380 390 400
QSSGVDTSPC PNSPVFRTGS EPALSPAVVR RVSSDARAGE ALRGSDSQLC
410 420 430 440 450
PKPPPKPCKV PFLKVPSSPS AWLNSEANYC ELNPAFATGC GRGAKLPSCA
460 470 480 490 500
QGSHTELLTA KQNEAPGPRN SGVNYLILDD DDRERPWEPA AAQMEKGQWD
510 520 530 540 550
KGEFVTPLLE TVSSFRPNEF ESKFLPPENK PLETAMLKRA KELFTNNDPK
560 570 580 590 600
VIAQHVLSMD CRVARILGVS EEMRRNMGVS SGLELITLPH GHQLRLDIIE
610 620 630 640 650
RHNTMAIGIA VDILGCTGTL EDRAATLSKI IQVAVELKDS MGDLYSFSAL
660 670 680 690 700
MKALEMPQIT RLEKTWTALR HQYTQTAILY EKQLKPFSKL LHEGRESTCV
710 720 730 740 750
PPNNVSVPLL MPLVTLMERQ AVTFEGTDMW EKNDQSCEIM LNHLATARFM
760 770 780 790 800
AEAADSYRMN AERILAGFQP DEEMNEICKT EFQMRLLWGS KGAQVNQTER
810 820
YEKFNQILTA LSRKLEPPPV KQAEL
Length:825
Mass (Da):92,566
Last modified:November 1, 1998 - v1
Checksum:iC55D61BB4AC7D0F2
GO
Isoform 2 (identifier: O75815-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-324: Missing.

Note: No experimental confirmation available.
Show »
Length:501
Mass (Da):55,745
Checksum:i50F3B53FB4BAF59E
GO
Isoform 3 (identifier: O75815-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-91: Missing.
     92-117: ESPWQDRHGETFTFRDPHLLDPTVEY → MPKECSAFHALSAALCCFYHRKSFIG

Note: No experimental confirmation available.
Show »
Length:734
Mass (Da):82,357
Checksum:iF48AF61CA484420A
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti519E → G in AL833121 (PubMed:17974005).Curated1
Sequence conflicti543L → P in AL833121 (PubMed:17974005).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_050689464E → G. Corresponds to variant dbSNP:rs12062278Ensembl.1
Natural variantiVAR_050690593Q → H. Corresponds to variant dbSNP:rs17110107Ensembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0178141 – 324Missing in isoform 2. 1 PublicationAdd BLAST324
Alternative sequenceiVSP_0467161 – 91Missing in isoform 3. 1 PublicationAdd BLAST91
Alternative sequenceiVSP_04671792 – 117ESPWQ…PTVEY → MPKECSAFHALSAALCCFYH RKSFIG in isoform 3. 1 PublicationAdd BLAST26

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U92715 mRNA Translation: AAC39777.1
AF124250 mRNA Translation: AAD28245.1
AY358996 mRNA Translation: AAQ89355.1
AL833121 mRNA No translation available.
AL049796 Genomic DNA No translation available.
AL109613 Genomic DNA No translation available.
AL359820 Genomic DNA No translation available.
AL512488 Genomic DNA No translation available.
CH471097 Genomic DNA Translation: EAW73065.1
CH471097 Genomic DNA Translation: EAW73066.1
CH471097 Genomic DNA Translation: EAW73068.1
BC039895 mRNA Translation: AAH39895.1
CCDSiCCDS58010.1 [O75815-3]
CCDS745.1 [O75815-1]
CCDS76181.1 [O75815-2]
RefSeqiNP_001248337.1, NM_001261408.1 [O75815-1]
NP_001248338.1, NM_001261409.1 [O75815-1]
NP_001248339.1, NM_001261410.1 [O75815-3]
NP_001295180.1, NM_001308251.1 [O75815-2]
NP_003558.1, NM_003567.3 [O75815-1]
XP_016857969.1, XM_017002480.1 [O75815-1]
UniGeneiHs.36958

Genome annotation databases

EnsembliENST00000260502; ENSP00000260502; ENSG00000137936 [O75815-1]
ENST00000370243; ENSP00000359263; ENSG00000137936 [O75815-1]
ENST00000370244; ENSP00000359264; ENSG00000137936 [O75815-1]
ENST00000370247; ENSP00000359267; ENSG00000137936 [O75815-3]
ENST00000539242; ENSP00000441343; ENSG00000137936 [O75815-2]
GeneIDi8412
KEGGihsa:8412
UCSCiuc001dpx.6 human [O75815-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U92715 mRNA Translation: AAC39777.1
AF124250 mRNA Translation: AAD28245.1
AY358996 mRNA Translation: AAQ89355.1
AL833121 mRNA No translation available.
AL049796 Genomic DNA No translation available.
AL109613 Genomic DNA No translation available.
AL359820 Genomic DNA No translation available.
AL512488 Genomic DNA No translation available.
CH471097 Genomic DNA Translation: EAW73065.1
CH471097 Genomic DNA Translation: EAW73066.1
CH471097 Genomic DNA Translation: EAW73068.1
BC039895 mRNA Translation: AAH39895.1
CCDSiCCDS58010.1 [O75815-3]
CCDS745.1 [O75815-1]
CCDS76181.1 [O75815-2]
RefSeqiNP_001248337.1, NM_001261408.1 [O75815-1]
NP_001248338.1, NM_001261409.1 [O75815-1]
NP_001248339.1, NM_001261410.1 [O75815-3]
NP_001295180.1, NM_001308251.1 [O75815-2]
NP_003558.1, NM_003567.3 [O75815-1]
XP_016857969.1, XM_017002480.1 [O75815-1]
UniGeneiHs.36958

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3T6AX-ray2.40A/B/C/D502-825[»]
ProteinModelPortaliO75815
SMRiO75815
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi114000, 38 interactors
DIPiDIP-33857N
IntActiO75815, 38 interactors
MINTiO75815
STRINGi9606.ENSP00000260502

PTM databases

iPTMnetiO75815
PhosphoSitePlusiO75815

Polymorphism and mutation databases

BioMutaiBCAR3

Proteomic databases

EPDiO75815
MaxQBiO75815
PaxDbiO75815
PeptideAtlasiO75815
PRIDEiO75815
ProteomicsDBi50205
50206 [O75815-2]

Protocols and materials databases

DNASUi8412
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000260502; ENSP00000260502; ENSG00000137936 [O75815-1]
ENST00000370243; ENSP00000359263; ENSG00000137936 [O75815-1]
ENST00000370244; ENSP00000359264; ENSG00000137936 [O75815-1]
ENST00000370247; ENSP00000359267; ENSG00000137936 [O75815-3]
ENST00000539242; ENSP00000441343; ENSG00000137936 [O75815-2]
GeneIDi8412
KEGGihsa:8412
UCSCiuc001dpx.6 human [O75815-1]

Organism-specific databases

CTDi8412
DisGeNETi8412
EuPathDBiHostDB:ENSG00000137936.16
GeneCardsiBCAR3
HGNCiHGNC:973 BCAR3
HPAiHPA014858
MIMi604704 gene
neXtProtiNX_O75815
OpenTargetsiENSG00000137936
PharmGKBiPA25283
GenAtlasiSearch...

Phylogenomic databases

eggNOGiENOG410IFQG Eukaryota
ENOG410XTJR LUCA
GeneTreeiENSGT00390000008976
HOGENOMiHOG000231595
HOVERGENiHBG053174
InParanoidiO75815
OMAiMDCKVAR
OrthoDBiEOG091G01KG
PhylomeDBiO75815
TreeFamiTF323756

Enzyme and pathway databases

SignaLinkiO75815

Miscellaneous databases

ChiTaRSiBCAR3 human
GeneWikiiBCAR3
GenomeRNAii8412
PROiPR:O75815
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000137936 Expressed in 222 organ(s), highest expression level in chorionic villus
CleanExiHS_BCAR3
GenevisibleiO75815 HS

Family and domain databases

CDDicd00155 RasGEF, 1 hit
Gene3Di1.10.840.10, 1 hit
3.30.505.10, 1 hit
InterProiView protein in InterPro
IPR028849 BCAR3
IPR023578 Ras_GEF_dom_sf
IPR001895 RASGEF_cat_dom
IPR036964 RASGEF_cat_dom_sf
IPR000980 SH2
IPR036860 SH2_dom_sf
PANTHERiPTHR14247:SF10 PTHR14247:SF10, 1 hit
PfamiView protein in Pfam
PF00617 RasGEF, 1 hit
PF00017 SH2, 1 hit
SMARTiView protein in SMART
SM00147 RasGEF, 1 hit
SM00252 SH2, 1 hit
SUPFAMiSSF48366 SSF48366, 1 hit
SSF55550 SSF55550, 1 hit
PROSITEiView protein in PROSITE
PS50009 RASGEF_CAT, 1 hit
PS50001 SH2, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiBCAR3_HUMAN
AccessioniPrimary (citable) accession number: O75815
Secondary accession number(s): D3DT43
, Q5TEW3, Q6UW40, Q9BR50
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 4, 2006
Last sequence update: November 1, 1998
Last modified: November 7, 2018
This is version 155 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 1
    Human chromosome 1: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
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Main funding by: National Institutes of Health

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