UniProtKB - O75689 (ADAP1_HUMAN)
Protein
Arf-GAP with dual PH domain-containing protein 1
Gene
ADAP1
Organism
Homo sapiens (Human)
Status
Functioni
GTPase-activating protein for the ADP ribosylation factor family (Probable). Binds phosphatidylinositol 3,4,5-trisphosphate (PtdInsP3) and inositol 1,3,4,5-tetrakisphosphate (InsP4).1 PublicationCurated1 Publication
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 21 – 44 | C4-typePROSITE-ProRule annotationAdd BLAST | 24 |
GO - Molecular functioni
- GTPase activator activity Source: UniProtKB
- inositol 1,3,4,5 tetrakisphosphate binding Source: UniProtKB
- metal ion binding Source: UniProtKB-KW
- phosphatidylinositol-3,4,5-trisphosphate binding Source: Ensembl
- phosphatidylinositol bisphosphate binding Source: InterPro
GO - Biological processi
- cell surface receptor signaling pathway Source: ProtInc
- regulation of GTPase activity Source: UniProtKB
Keywordsi
Molecular function | GTPase activation |
Ligand | Metal-binding, Zinc |
Enzyme and pathway databases
PathwayCommonsi | O75689 |
Reactomei | R-HSA-1251985, Nuclear signaling by ERBB4 |
SIGNORi | O75689 |
Names & Taxonomyi
Protein namesi | Recommended name: Arf-GAP with dual PH domain-containing protein 1Alternative name(s): Centaurin-alpha-1 Short name: Cnt-a1 Putative MAPK-activating protein PM25 |
Gene namesi | Name:ADAP1 Synonyms:CENTA1 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:16486, ADAP1 |
MIMi | 608114, gene |
neXtProti | NX_O75689 |
VEuPathDBi | HostDB:ENSG00000105963.13 |
Subcellular locationi
Cytosol
- cytosol Source: HPA
Nucleus
- nucleus Source: UniProtKB
Plasma Membrane
- plasma membrane Source: UniProtKB
Other locations
- cytoplasm Source: UniProtKB
- intracellular membrane-bounded organelle Source: GO_Central
Keywords - Cellular componenti
Cytoplasm, NucleusPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 21 | C → A: Loss of GTPase-activating activity. 1 Publication | 1 | |
Mutagenesisi | 24 | C → A: Loss of GTPase-activating activity. 1 Publication | 1 | |
Mutagenesisi | 149 | R → C: 40-45% reduction in PtdInsP2 3-kinase dependent membrane localization. Almost complete loss of PtdInsP2 3-kinase dependent membrane localization; when associated with C-273. 2 Publications | 1 | |
Mutagenesisi | 273 | R → C: 70% reduction in PtdInsP2 3-kinase dependent membrane localization. Almost complete loss of PtdInsP2 3-kinase dependent membrane localization; when associated with C-149. 2 Publications | 1 |
Organism-specific databases
DisGeNETi | 11033 |
OpenTargetsi | ENSG00000105963 |
PharmGKBi | PA26404 |
Miscellaneous databases
Pharosi | O75689, Tbio |
Genetic variation databases
BioMutai | ADAP1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000074205 | 1 – 374 | Arf-GAP with dual PH domain-containing protein 1Add BLAST | 374 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 87 | Phosphoserine; by PKC1 Publication | 1 | |
Modified residuei | 272 | N6-acetyllysineCombined sources | 1 | |
Modified residuei | 276 | Phosphothreonine; by PKC1 Publication | 1 |
Post-translational modificationi
Phosphorylated by PRKCA, PRKCI, PRKCZ and PRKD1 in vitro.1 Publication
Keywords - PTMi
Acetylation, PhosphoproteinProteomic databases
EPDi | O75689 |
jPOSTi | O75689 |
MassIVEi | O75689 |
MaxQBi | O75689 |
PaxDbi | O75689 |
PeptideAtlasi | O75689 |
PRIDEi | O75689 |
ProteomicsDBi | 28173 45067 50162 [O75689-1] |
PTM databases
iPTMneti | O75689 |
PhosphoSitePlusi | O75689 |
Expressioni
Tissue specificityi
Expressed at highest levels in brain and at lower levels in peripheral blood leukocytes.1 Publication
Gene expression databases
Bgeei | ENSG00000105963, Expressed in C1 segment of cervical spinal cord and 114 other tissues |
ExpressionAtlasi | O75689, baseline and differential |
Genevisiblei | O75689, HS |
Organism-specific databases
HPAi | ENSG00000105963, Tissue enhanced (brain, intestine) |
Interactioni
Subunit structurei
Interacts with PRKCA, PRKCI and PRKCZ.
Interacts with the N-terminal region of PRKD1.
1 PublicationBinary interactionsi
Hide detailsO75689
With | #Exp. | IntAct |
---|---|---|
DVL3 [Q92997] | 3 | EBI-714732,EBI-739789 |
GOLGA2 [Q08379] | 3 | EBI-714732,EBI-618309 |
KIF13B [Q9NQT8] | 6 | EBI-714732,EBI-766408 |
PLEKHF2 [Q9H8W4] | 3 | EBI-714732,EBI-742388 |
SDCBP2 [Q9H190] | 3 | EBI-714732,EBI-742426 |
Protein-protein interaction databases
BioGRIDi | 116222, 20 interactors |
DIPi | DIP-41731N |
IntActi | O75689, 11 interactors |
MINTi | O75689 |
STRINGi | 9606.ENSP00000442682 |
Miscellaneous databases
RNActi | O75689, protein |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | O75689 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | O75689 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 7 – 126 | Arf-GAPPROSITE-ProRule annotationAdd BLAST | 120 | |
Domaini | 129 – 230 | PH 1PROSITE-ProRule annotationAdd BLAST | 102 | |
Domaini | 252 – 356 | PH 2PROSITE-ProRule annotationAdd BLAST | 105 |
Zinc finger
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 21 – 44 | C4-typePROSITE-ProRule annotationAdd BLAST | 24 |
Keywords - Domaini
Repeat, Zinc-fingerPhylogenomic databases
eggNOGi | KOG0703, Eukaryota |
GeneTreei | ENSGT00940000155698 |
HOGENOMi | CLU_061583_0_0_1 |
InParanoidi | O75689 |
OMAi | YEERVPP |
OrthoDBi | 1274375at2759 |
PhylomeDBi | O75689 |
TreeFami | TF324540 |
Family and domain databases
CDDi | cd13252, PH1_ADAP, 1 hit cd01251, PH2_ADAP, 1 hit |
Gene3Di | 2.30.29.30, 2 hits 3.30.40.160, 1 hit |
InterProi | View protein in InterPro IPR037278, ARFGAP/RecO IPR001164, ArfGAP_dom IPR038508, ArfGAP_dom_sf IPR011993, PH-like_dom_sf IPR037849, PH1_ADAP IPR037851, PH2_ADAP IPR001849, PH_domain |
Pfami | View protein in Pfam PF01412, ArfGap, 1 hit PF00169, PH, 2 hits |
PRINTSi | PR00405, REVINTRACTNG |
SMARTi | View protein in SMART SM00105, ArfGap, 1 hit SM00233, PH, 2 hits |
SUPFAMi | SSF57863, SSF57863, 1 hit |
PROSITEi | View protein in PROSITE PS50115, ARFGAP, 1 hit PS50003, PH_DOMAIN, 2 hits |
s (3+)i Sequence
Sequence statusi: Complete.
This entry describes 3 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 3 described isoforms and 8 potential isoforms that are computationally mapped.Show allAlign All
Isoform 1 (identifier: O75689-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MAKERRRAVL ELLQRPGNAR CADCGAPDPD WASYTLGVFI CLSCSGIHRN
60 70 80 90 100
IPQVSKVKSV RLDAWEEAQV EFMASHGNDA ARARFESKVP SFYYRPTPSD
110 120 130 140 150
CQLLREQWIR AKYERQEFIY PEKQEPYSAG YREGFLWKRG RDNGQFLSRK
160 170 180 190 200
FVLTEREGAL KYFNRNDAKE PKAVMKIEHL NATFQPAKIG HPHGLQVTYL
210 220 230 240 250
KDNSTRNIFI YHEDGKEIVD WFNALRAARF HYLQVAFPGA GDADLVPKLS
260 270 280 290 300
RNYLKEGYME KTGPKQTEGF RKRWFTMDDR RLMYFKDPLD AFARGEVFIG
310 320 330 340 350
SKESGYTVLH GFPPSTQGHH WPHGITIVTP DRKFLFACET ESDQREWVAA
360 370
FQKAVDRPML PQEYAVEAHF KHKP
Computationally mapped potential isoform sequencesi
There are 8 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketH0Y7Q1 | H0Y7Q1_HUMAN | Arf-GAP with dual PH domain-contain... | ADAP1 | 357 | Annotation score: | ||
H7C324 | H7C324_HUMAN | Arf-GAP with dual PH domain-contain... | ADAP1 | 245 | Annotation score: | ||
A0A087WTN6 | A0A087WTN6_HUMAN | Arf-GAP with dual PH domain-contain... | ADAP1 | 279 | Annotation score: | ||
H7BZT6 | H7BZT6_HUMAN | Arf-GAP with dual PH domain-contain... | ADAP1 | 192 | Annotation score: | ||
H7C264 | H7C264_HUMAN | Arf-GAP with dual PH domain-contain... | ADAP1 | 207 | Annotation score: | ||
H7C4B3 | H7C4B3_HUMAN | Arf-GAP with dual PH domain-contain... | ADAP1 | 187 | Annotation score: | ||
A0A3B3ISA7 | A0A3B3ISA7_HUMAN | Arf-GAP with dual PH domain-contain... | ADAP1 | 373 | Annotation score: | ||
C9JN36 | C9JN36_HUMAN | Arf-GAP with dual PH domain-contain... | ADAP1 | 162 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 215 | G → R in BAG52556 (PubMed:14702039).Curated | 1 |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_047470 | 241 | G → S4 PublicationsCorresponds to variant dbSNP:rs10256887Ensembl. | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_054793 | 1 – 72 | Missing in isoform 3. 1 PublicationAdd BLAST | 72 | |
Alternative sequenceiVSP_054794 | 1 – 27 | MAKER…DCGAP → MFQFVFSRVYCINPARRKWK EFEKMLGCAEEGHASLGR in isoform 2. 1 PublicationAdd BLAST | 27 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AJ006422 mRNA Translation: CAA07024.1 AF082324 mRNA Translation: AAD11414.1 AB097049 mRNA Translation: BAC77402.1 AK300999 mRNA Translation: BAG62618.1 AC073957 Genomic DNA No translation available. AK092471 mRNA Translation: BAG52556.1 CH236965 Genomic DNA Translation: EAL23709.1 CH471144 Genomic DNA Translation: EAW87183.1 CH471144 Genomic DNA Translation: EAW87184.1 BC033747 mRNA Translation: AAH33747.1 |
CCDSi | CCDS5318.1 [O75689-1] CCDS64576.1 [O75689-3] CCDS64577.1 [O75689-2] |
PIRi | JC7091 |
RefSeqi | NP_001271237.1, NM_001284308.1 NP_001271238.1, NM_001284309.1 [O75689-3] NP_001271239.1, NM_001284310.1 NP_001271240.1, NM_001284311.1 NP_006860.1, NM_006869.3 [O75689-1] |
Genome annotation databases
Ensembli | ENST00000265846; ENSP00000265846; ENSG00000105963 [O75689-1] ENST00000449296; ENSP00000407267; ENSG00000105963 [O75689-3] ENST00000539900; ENSP00000442682; ENSG00000105963 [O75689-2] ENST00000611167; ENSP00000481154; ENSG00000105963 [O75689-3] |
GeneIDi | 11033 |
KEGGi | hsa:11033 |
UCSCi | uc003sjn.5, human [O75689-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AJ006422 mRNA Translation: CAA07024.1 AF082324 mRNA Translation: AAD11414.1 AB097049 mRNA Translation: BAC77402.1 AK300999 mRNA Translation: BAG62618.1 AC073957 Genomic DNA No translation available. AK092471 mRNA Translation: BAG52556.1 CH236965 Genomic DNA Translation: EAL23709.1 CH471144 Genomic DNA Translation: EAW87183.1 CH471144 Genomic DNA Translation: EAW87184.1 BC033747 mRNA Translation: AAH33747.1 |
CCDSi | CCDS5318.1 [O75689-1] CCDS64576.1 [O75689-3] CCDS64577.1 [O75689-2] |
PIRi | JC7091 |
RefSeqi | NP_001271237.1, NM_001284308.1 NP_001271238.1, NM_001284309.1 [O75689-3] NP_001271239.1, NM_001284310.1 NP_001271240.1, NM_001284311.1 NP_006860.1, NM_006869.3 [O75689-1] |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
3FEH | X-ray | 1.90 | A | 3-370 | [»] | |
3FM8 | X-ray | 2.30 | C/D | 1-374 | [»] | |
3LJU | X-ray | 1.70 | X | 3-370 | [»] | |
3MDB | X-ray | 2.95 | C/D | 1-374 | [»] | |
SMRi | O75689 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 116222, 20 interactors |
DIPi | DIP-41731N |
IntActi | O75689, 11 interactors |
MINTi | O75689 |
STRINGi | 9606.ENSP00000442682 |
PTM databases
iPTMneti | O75689 |
PhosphoSitePlusi | O75689 |
Genetic variation databases
BioMutai | ADAP1 |
Proteomic databases
EPDi | O75689 |
jPOSTi | O75689 |
MassIVEi | O75689 |
MaxQBi | O75689 |
PaxDbi | O75689 |
PeptideAtlasi | O75689 |
PRIDEi | O75689 |
ProteomicsDBi | 28173 45067 50162 [O75689-1] |
Protocols and materials databases
Antibodypediai | 1977, 215 antibodies |
Genome annotation databases
Ensembli | ENST00000265846; ENSP00000265846; ENSG00000105963 [O75689-1] ENST00000449296; ENSP00000407267; ENSG00000105963 [O75689-3] ENST00000539900; ENSP00000442682; ENSG00000105963 [O75689-2] ENST00000611167; ENSP00000481154; ENSG00000105963 [O75689-3] |
GeneIDi | 11033 |
KEGGi | hsa:11033 |
UCSCi | uc003sjn.5, human [O75689-1] |
Organism-specific databases
CTDi | 11033 |
DisGeNETi | 11033 |
GeneCardsi | ADAP1 |
HGNCi | HGNC:16486, ADAP1 |
HPAi | ENSG00000105963, Tissue enhanced (brain, intestine) |
MIMi | 608114, gene |
neXtProti | NX_O75689 |
OpenTargetsi | ENSG00000105963 |
PharmGKBi | PA26404 |
VEuPathDBi | HostDB:ENSG00000105963.13 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG0703, Eukaryota |
GeneTreei | ENSGT00940000155698 |
HOGENOMi | CLU_061583_0_0_1 |
InParanoidi | O75689 |
OMAi | YEERVPP |
OrthoDBi | 1274375at2759 |
PhylomeDBi | O75689 |
TreeFami | TF324540 |
Enzyme and pathway databases
PathwayCommonsi | O75689 |
Reactomei | R-HSA-1251985, Nuclear signaling by ERBB4 |
SIGNORi | O75689 |
Miscellaneous databases
BioGRID-ORCSi | 11033, 13 hits in 874 CRISPR screens |
ChiTaRSi | ADAP1, human |
EvolutionaryTracei | O75689 |
GeneWikii | Centaurin,_alpha_1 |
GenomeRNAii | 11033 |
Pharosi | O75689, Tbio |
PROi | PR:O75689 |
RNActi | O75689, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000105963, Expressed in C1 segment of cervical spinal cord and 114 other tissues |
ExpressionAtlasi | O75689, baseline and differential |
Genevisiblei | O75689, HS |
Family and domain databases
CDDi | cd13252, PH1_ADAP, 1 hit cd01251, PH2_ADAP, 1 hit |
Gene3Di | 2.30.29.30, 2 hits 3.30.40.160, 1 hit |
InterProi | View protein in InterPro IPR037278, ARFGAP/RecO IPR001164, ArfGAP_dom IPR038508, ArfGAP_dom_sf IPR011993, PH-like_dom_sf IPR037849, PH1_ADAP IPR037851, PH2_ADAP IPR001849, PH_domain |
Pfami | View protein in Pfam PF01412, ArfGap, 1 hit PF00169, PH, 2 hits |
PRINTSi | PR00405, REVINTRACTNG |
SMARTi | View protein in SMART SM00105, ArfGap, 1 hit SM00233, PH, 2 hits |
SUPFAMi | SSF57863, SSF57863, 1 hit |
PROSITEi | View protein in PROSITE PS50115, ARFGAP, 1 hit PS50003, PH_DOMAIN, 2 hits |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | ADAP1_HUMAN | |
Accessioni | O75689Primary (citable) accession number: O75689 Secondary accession number(s): A4D2Q2 H7C2Q4 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 5, 2005 |
Last sequence update: | November 25, 2008 | |
Last modified: | February 10, 2021 | |
This is version 172 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Human chromosome 7
Human chromosome 7: entries, gene names and cross-references to MIM - Human entries with genetic variants
List of human entries with genetic variants - Human variants curated from literature reports
Index of human variants curated from literature reports - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references