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Protein

Protein phosphatase 1B

Gene

PPM1B

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Enzyme with a broad specificity. Dephosphorylates CDK2 and CDK6 in vitro. Dephosphorylates PRKAA1 and PRKAA2. Inhibits TBK1-mediated antiviral signaling by dephosphorylating it at 'Ser-172'. Plays an important role in the termination of TNF-alpha-mediated NF-kappa-B activation through dephosphorylating and inactivating IKBKB/IKKB.2 Publications

Catalytic activityi

[a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

Cofactori

Mg2+By similarity, Mn2+By similarityNote: Binds 2 magnesium or manganese ions per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi60Manganese 1By similarity1
Metal bindingi60Manganese 2Combined sources1 Publication1
Metal bindingi61Manganese 1; via carbonyl oxygenBy similarity1
Metal bindingi243Manganese 2Combined sources1 Publication1
Metal bindingi286Manganese 2Combined sources1 Publication1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase, Protein phosphatase
LigandMagnesium, Manganese, Metal-binding

Enzyme and pathway databases

ReactomeiR-HSA-1169408 ISG15 antiviral mechanism
SIGNORiO75688

Names & Taxonomyi

Protein namesi
Recommended name:
Protein phosphatase 1B (EC:3.1.3.16)
Alternative name(s):
Protein phosphatase 2C isoform beta
Short name:
PP2C-beta
Gene namesi
Name:PPM1B
Synonyms:PP2CB
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 2

Organism-specific databases

EuPathDBiHostDB:ENSG00000138032.20
HGNCiHGNC:9276 PPM1B
MIMi603770 gene
neXtProtiNX_O75688

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Membrane

Pathology & Biotechi

Organism-specific databases

DisGeNETi5495
MalaCardsiPPM1B
OpenTargetsiENSG00000138032
Orphaneti163693 2p21 microdeletion syndrome
PharmGKBiPA33604

Chemistry databases

ChEMBLiCHEMBL2845

Polymorphism and mutation databases

BioMutaiPPM1B

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved1 Publication
ChainiPRO_00000577462 – 479Protein phosphatase 1BAdd BLAST478

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Lipidationi2N-myristoyl glycine1 Publication1
Cross-linki12Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ISG15)
Cross-linki142Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ISG15)
Modified residuei386PhosphoserineCombined sources1

Post-translational modificationi

Isgylation negatively regulates its activity.1 Publication
N-myristoylation is essential for the recognition of its substrates for dephosphorylation.By similarity

Keywords - PTMi

Isopeptide bond, Lipoprotein, Myristate, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiO75688
PaxDbiO75688
PeptideAtlasiO75688
PRIDEiO75688
ProteomicsDBi50157
50158 [O75688-2]
50159 [O75688-3]
50160 [O75688-4]
50161 [O75688-5]

PTM databases

DEPODiO75688
iPTMnetiO75688
PhosphoSitePlusiO75688

Expressioni

Tissue specificityi

Highly expressed in heart and skeletal muscle.

Gene expression databases

BgeeiENSG00000138032 Expressed in 235 organ(s), highest expression level in secondary oocyte
CleanExiHS_PPM1B
ExpressionAtlasiO75688 baseline and differential
GenevisibleiO75688 HS

Organism-specific databases

HPAiHPA016745

Interactioni

Subunit structurei

Monomer (By similarity). Interacts with PAK6. Interacts with the phosphorylated form of IKBKB/IKKB.By similarity2 Publications

Binary interactionsi

Protein-protein interaction databases

BioGridi111490, 94 interactors
IntActiO75688, 33 interactors
MINTiO75688
STRINGi9606.ENSP00000282412

Chemistry databases

BindingDBiO75688

Structurei

Secondary structure

1479
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliO75688
SMRiO75688
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO75688

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini23 – 295PPM-type phosphatasePROSITE-ProRule annotationAdd BLAST273

Sequence similaritiesi

Belongs to the PP2C family.Curated

Phylogenomic databases

eggNOGiKOG0697 Eukaryota
COG0631 LUCA
GeneTreeiENSGT00880000137909
HOGENOMiHOG000233895
HOVERGENiHBG053647
InParanoidiO75688
KOiK04461
OMAiARVSAHC
OrthoDBiEOG091G0AJQ
PhylomeDBiO75688
TreeFamiTF313590

Family and domain databases

CDDicd00143 PP2Cc, 1 hit
Gene3Di1.10.10.430, 1 hit
3.60.40.10, 1 hit
InterProiView protein in InterPro
IPR015655 PP2C
IPR000222 PP2C_BS
IPR012911 PP2C_C
IPR036580 PP2C_C_sf
IPR036457 PPM-type_dom_sf
IPR001932 PPM-type_phosphatase_dom
PANTHERiPTHR13832 PTHR13832, 1 hit
PfamiView protein in Pfam
PF00481 PP2C, 1 hit
PF07830 PP2C_C, 1 hit
SMARTiView protein in SMART
SM00332 PP2Cc, 1 hit
SUPFAMiSSF81601 SSF81601, 1 hit
SSF81606 SSF81606, 1 hit
PROSITEiView protein in PROSITE
PS01032 PPM_1, 1 hit
PS51746 PPM_2, 1 hit

Sequences (5+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 5 isoformsi produced by alternative splicing. AlignAdd to basket
Note: Additional isoforms seem to exist.

This entry has 5 described isoforms and 2 potential isoforms that are computationally mapped.Show allAlign All

Isoform Beta-1 (identifier: O75688-1) [UniParc]FASTAAdd to basket
Also known as: Beta-X, PPM1B2

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MGAFLDKPKT EKHNAHGAGN GLRYGLSSMQ GWRVEMEDAH TAVVGIPHGL
60 70 80 90 100
EDWSFFAVYD GHAGSRVANY CSTHLLEHIT TNEDFRAAGK SGSALELSVE
110 120 130 140 150
NVKNGIRTGF LKIDEYMRNF SDLRNGMDRS GSTAVGVMIS PKHIYFINCG
160 170 180 190 200
DSRAVLYRNG QVCFSTQDHK PCNPREKERI QNAGGSVMIQ RVNGSLAVSR
210 220 230 240 250
ALGDYDYKCV DGKGPTEQLV SPEPEVYEIL RAEEDEFIIL ACDGIWDVMS
260 270 280 290 300
NEELCEYVKS RLEVSDDLEN VCNWVVDTCL HKGSRDNMSI VLVCFSNAPK
310 320 330 340 350
VSDEAVKKDS ELDKHLESRV EEIMEKSGEE GMPDLAHVMR ILSAENIPNL
360 370 380 390 400
PPGGGLAGKR NVIEAVYSRL NPHRESDGAS DEAEESGSQG KLVEALRQMR
410 420 430 440 450
INHRGNYRQL LEEMLTSYRL AKVEGEESPA EPAATATSSN SDAGNPVTMQ
460 470
ESHTESESGL AELDSSNEDA GTKMSGEKI
Length:479
Mass (Da):52,643
Last modified:November 1, 1998 - v1
Checksum:iA3A5797AD263DFBD
GO
Isoform Beta-2 (identifier: O75688-2) [UniParc]FASTAAdd to basket
Also known as: PPM1B1

The sequence of this isoform differs from the canonical sequence as follows:
     379-387: ASDEAEESG → GAGDLEDPW
     388-479: Missing.

Show »
Length:387
Mass (Da):42,771
Checksum:i56A644A4E5E2B12B
GO
Isoform Beta-X (identifier: O75688-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-287: Missing.

Show »
Length:192
Mass (Da):20,753
Checksum:i46FA14838483534F
GO
Isoform 4 (identifier: O75688-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     379-380: AS → QK
     381-387: Missing.
     388-479: Missing.

Show »
Length:380
Mass (Da):42,086
Checksum:iE5C00B5FF4278013
GO
Isoform 5 (identifier: O75688-5) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     322-327: EIMEKS → GKTNAF
     328-380: Missing.
     381-387: Missing.
     388-479: Missing.

Show »
Length:327
Mass (Da):36,313
Checksum:iE38A263FCF7F1692
GO

Computationally mapped potential isoform sequencesi

There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
C9JIR6C9JIR6_HUMAN
Protein phosphatase 1B
PPM1B
378Annotation score:
B8ZZF0B8ZZF0_HUMAN
Protein phosphatase 1B
PPM1B
303Annotation score:

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0410851 – 287Missing in isoform Beta-X. 1 PublicationAdd BLAST287
Alternative sequenceiVSP_043641322 – 327EIMEKS → GKTNAF in isoform 5. 1 Publication6
Alternative sequenceiVSP_043642328 – 380Missing in isoform 5. 1 PublicationAdd BLAST53
Alternative sequenceiVSP_005087379 – 387ASDEAEESG → GAGDLEDPW in isoform Beta-2. 4 Publications9
Alternative sequenceiVSP_043643379 – 380AS → QK in isoform 4. 1 Publication2
Alternative sequenceiVSP_043644381 – 387Missing in isoform 4 and isoform 5. 1 Publication7
Alternative sequenceiVSP_005088388 – 479Missing in isoform Beta-2, isoform 4 and isoform 5. 4 PublicationsAdd BLAST92

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ005801 mRNA Translation: CAA06704.1
AF294792 mRNA Translation: AAG02232.1
AJ271832 mRNA Translation: CAC27992.1
AJ271835 mRNA Translation: CAC27993.1
DQ023508 mRNA Translation: AAY89639.1
DQ023509 mRNA Translation: AAY89640.1
DQ023510 mRNA Translation: AAY89641.1
AF136972 mRNA Translation: AAG49433.1
AL833035 mRNA Translation: CAH56319.1
AC013717 Genomic DNA Translation: AAX88954.1
AC019129 Genomic DNA No translation available.
CH471053 Genomic DNA Translation: EAX00282.1
CH471053 Genomic DNA Translation: EAX00283.1
BC012002 mRNA Translation: AAH12002.1
BC064381 mRNA Translation: AAH64381.1
CCDSiCCDS1816.1 [O75688-2]
CCDS1817.1 [O75688-1]
CCDS1818.1 [O75688-3]
CCDS46271.1 [O75688-4]
RefSeqiNP_001028729.1, NM_001033557.2 [O75688-4]
NP_002697.1, NM_002706.5 [O75688-1]
NP_808907.1, NM_177968.3 [O75688-2]
NP_808908.1, NM_177969.3 [O75688-3]
UniGeneiHs.416769

Genome annotation databases

EnsembliENST00000282412; ENSP00000282412; ENSG00000138032 [O75688-1]
ENST00000345249; ENSP00000326089; ENSG00000138032 [O75688-3]
ENST00000378551; ENSP00000367813; ENSG00000138032 [O75688-2]
ENST00000409432; ENSP00000387287; ENSG00000138032 [O75688-4]
GeneIDi5495
KEGGihsa:5495
UCSCiuc002rtt.4 human [O75688-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ005801 mRNA Translation: CAA06704.1
AF294792 mRNA Translation: AAG02232.1
AJ271832 mRNA Translation: CAC27992.1
AJ271835 mRNA Translation: CAC27993.1
DQ023508 mRNA Translation: AAY89639.1
DQ023509 mRNA Translation: AAY89640.1
DQ023510 mRNA Translation: AAY89641.1
AF136972 mRNA Translation: AAG49433.1
AL833035 mRNA Translation: CAH56319.1
AC013717 Genomic DNA Translation: AAX88954.1
AC019129 Genomic DNA No translation available.
CH471053 Genomic DNA Translation: EAX00282.1
CH471053 Genomic DNA Translation: EAX00283.1
BC012002 mRNA Translation: AAH12002.1
BC064381 mRNA Translation: AAH64381.1
CCDSiCCDS1816.1 [O75688-2]
CCDS1817.1 [O75688-1]
CCDS1818.1 [O75688-3]
CCDS46271.1 [O75688-4]
RefSeqiNP_001028729.1, NM_001033557.2 [O75688-4]
NP_002697.1, NM_002706.5 [O75688-1]
NP_808907.1, NM_177968.3 [O75688-2]
NP_808908.1, NM_177969.3 [O75688-3]
UniGeneiHs.416769

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2P8EX-ray1.82A/B2-297[»]
ProteinModelPortaliO75688
SMRiO75688
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi111490, 94 interactors
IntActiO75688, 33 interactors
MINTiO75688
STRINGi9606.ENSP00000282412

Chemistry databases

BindingDBiO75688
ChEMBLiCHEMBL2845

PTM databases

DEPODiO75688
iPTMnetiO75688
PhosphoSitePlusiO75688

Polymorphism and mutation databases

BioMutaiPPM1B

Proteomic databases

EPDiO75688
PaxDbiO75688
PeptideAtlasiO75688
PRIDEiO75688
ProteomicsDBi50157
50158 [O75688-2]
50159 [O75688-3]
50160 [O75688-4]
50161 [O75688-5]

Protocols and materials databases

DNASUi5495
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000282412; ENSP00000282412; ENSG00000138032 [O75688-1]
ENST00000345249; ENSP00000326089; ENSG00000138032 [O75688-3]
ENST00000378551; ENSP00000367813; ENSG00000138032 [O75688-2]
ENST00000409432; ENSP00000387287; ENSG00000138032 [O75688-4]
GeneIDi5495
KEGGihsa:5495
UCSCiuc002rtt.4 human [O75688-1]

Organism-specific databases

CTDi5495
DisGeNETi5495
EuPathDBiHostDB:ENSG00000138032.20
GeneCardsiPPM1B
HGNCiHGNC:9276 PPM1B
HPAiHPA016745
MalaCardsiPPM1B
MIMi603770 gene
neXtProtiNX_O75688
OpenTargetsiENSG00000138032
Orphaneti163693 2p21 microdeletion syndrome
PharmGKBiPA33604
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG0697 Eukaryota
COG0631 LUCA
GeneTreeiENSGT00880000137909
HOGENOMiHOG000233895
HOVERGENiHBG053647
InParanoidiO75688
KOiK04461
OMAiARVSAHC
OrthoDBiEOG091G0AJQ
PhylomeDBiO75688
TreeFamiTF313590

Enzyme and pathway databases

ReactomeiR-HSA-1169408 ISG15 antiviral mechanism
SIGNORiO75688

Miscellaneous databases

ChiTaRSiPPM1B human
EvolutionaryTraceiO75688
GeneWikiiPPM1B
GenomeRNAii5495
PROiPR:O75688
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000138032 Expressed in 235 organ(s), highest expression level in secondary oocyte
CleanExiHS_PPM1B
ExpressionAtlasiO75688 baseline and differential
GenevisibleiO75688 HS

Family and domain databases

CDDicd00143 PP2Cc, 1 hit
Gene3Di1.10.10.430, 1 hit
3.60.40.10, 1 hit
InterProiView protein in InterPro
IPR015655 PP2C
IPR000222 PP2C_BS
IPR012911 PP2C_C
IPR036580 PP2C_C_sf
IPR036457 PPM-type_dom_sf
IPR001932 PPM-type_phosphatase_dom
PANTHERiPTHR13832 PTHR13832, 1 hit
PfamiView protein in Pfam
PF00481 PP2C, 1 hit
PF07830 PP2C_C, 1 hit
SMARTiView protein in SMART
SM00332 PP2Cc, 1 hit
SUPFAMiSSF81601 SSF81601, 1 hit
SSF81606 SSF81606, 1 hit
PROSITEiView protein in PROSITE
PS01032 PPM_1, 1 hit
PS51746 PPM_2, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiPPM1B_HUMAN
AccessioniPrimary (citable) accession number: O75688
Secondary accession number(s): Q461Q2
, Q4J6C1, Q4J6C2, Q658R4, Q96ER6, Q9HAY8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: November 1, 1998
Last modified: September 12, 2018
This is version 188 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Human chromosome 2
    Human chromosome 2: entries, gene names and cross-references to MIM
  3. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  4. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
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