UniProtKB - O75677 (RFPL1_HUMAN)
Protein
Ret finger protein-like 1
Gene
RFPL1
Organism
Homo sapiens (Human)
Status
Functioni
Negatively regulates the G2-M phase transition, possibly by promoting cyclin B1/CCNB1 and CDK1 proteasomal degradation and thereby preventing their accumulation during interphase.1 Publication
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 40 – 82 | RING-typePROSITE-ProRule annotationAdd BLAST | 43 |
GO - Molecular functioni
- metal ion binding Source: UniProtKB-KW
- ubiquitin-protein transferase activity Source: GO_Central
GO - Biological processi
- cell cycle Source: UniProtKB-KW
- negative regulation of cell division Source: UniProtKB
- negative regulation of cell population proliferation Source: UniProtKB
- negative regulation of G2/M transition of mitotic cell cycle Source: UniProtKB
- negative regulation of mitotic cell cycle Source: UniProtKB
- positive regulation of apoptotic process Source: UniProtKB
- positive regulation of cysteine-type endopeptidase activity involved in execution phase of apoptosis Source: UniProtKB
- positive regulation of proteasomal ubiquitin-dependent protein catabolic process Source: UniProtKB
- positive regulation of transcription, DNA-templated Source: GO_Central
Keywordsi
Biological process | Cell cycle |
Ligand | Metal-binding, Zinc |
Enzyme and pathway databases
PathwayCommonsi | O75677 |
Names & Taxonomyi
Protein namesi | Recommended name: Ret finger protein-like 1Alternative name(s): RING finger protein 78 |
Gene namesi | Name:RFPL1 Synonyms:RFPL1L, RNF78 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
EuPathDBi | HostDB:ENSG00000128250.5 |
HGNCi | HGNC:9977, RFPL1 |
MIMi | 605968, gene |
neXtProti | NX_O75677 |
Subcellular locationi
Nucleus
- nucleoplasm Source: GO_Central
Other locations
Keywords - Cellular componenti
Cytoplasm, NucleusPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 65 – 124 | Missing : Increased cell proliferation. 1 PublicationAdd BLAST | 60 | |
Mutagenesisi | 200 | S → A: Reduced phosphorylation by PKC, increased cell proliferation, reduced apoptosis and lack of cyclin B1/CCNB1 and CDK1 degradation. 1 Publication | 1 | |
Mutagenesisi | 200 | S → E: No effect on cell proliferation. 1 Publication | 1 | |
Mutagenesisi | 287 | S → E: Increased cell proliferation, reduced apoptosis and lack of cyclin B1/CCNB1 and CDK1 degradation. 1 Publication | 1 | |
Mutagenesisi | 287 | S → G: Reduced phosphorylation by CDK1. 1 Publication | 1 |
Organism-specific databases
DisGeNETi | 5988 |
OpenTargetsi | ENSG00000128250 |
PharmGKBi | PA34346 |
Miscellaneous databases
Pharosi | O75677, Tdark |
Polymorphism and mutation databases
BioMutai | RFPL1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000056030 | 1 – 317 | Ret finger protein-like 1Add BLAST | 317 |
Post-translational modificationi
Phosphorylated by PKC and CDK1 (PubMed:20725088). The antiproliferative effect seems to be positively regulated by PKC phosphorylation and negatively by CDK1 phosphorylation (PubMed:20725088).1 Publication
Keywords - PTMi
PhosphoproteinProteomic databases
jPOSTi | O75677 |
PaxDbi | O75677 |
PeptideAtlasi | O75677 |
PRIDEi | O75677 |
ProteomicsDBi | 50151 |
PTM databases
iPTMneti | O75677 |
PhosphoSitePlusi | O75677 |
Expressioni
Tissue specificityi
Seems to be expressed in prostate and less abundantly in adult brain, fetal liver, and fetal kidney.
Gene expression databases
Bgeei | ENSG00000128250, Expressed in prefrontal cortex and 60 other tissues |
Genevisiblei | O75677, HS |
Organism-specific databases
HPAi | ENSG00000128250, Tissue enriched (brain) |
Interactioni
Protein-protein interaction databases
BioGRIDi | 111920, 1 interactor |
IntActi | O75677, 1 interactor |
STRINGi | 9606.ENSP00000346342 |
Miscellaneous databases
RNActi | O75677, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 107 – 301 | B30.2/SPRYPROSITE-ProRule annotationAdd BLAST | 195 |
Domaini
The B30.2/SPRY domain is required for the negative regulation of cell proliferation.1 Publication
Zinc finger
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 40 – 82 | RING-typePROSITE-ProRule annotationAdd BLAST | 43 |
Keywords - Domaini
Zinc-fingerPhylogenomic databases
eggNOGi | KOG2177, Eukaryota |
GeneTreei | ENSGT00940000163408 |
HOGENOMi | CLU_013137_7_1_1 |
InParanoidi | O75677 |
OMAi | CDIHYES |
OrthoDBi | 1139938at2759 |
PhylomeDBi | O75677 |
TreeFami | TF317532 |
Family and domain databases
CDDi | cd15821, SPRY_PRY_RFPL, 1 hit |
Gene3Di | 2.60.120.920, 1 hit 3.30.40.10, 1 hit |
InterProi | View protein in InterPro IPR001870, B30.2/SPRY IPR043136, B30.2/SPRY_sf IPR003879, Butyrophylin_SPRY IPR013320, ConA-like_dom_sf IPR006574, PRY IPR022723, RDM_domain_RFPL IPR038908, RFPL IPR037960, SPRY/PRY_RFPL IPR003877, SPRY_dom IPR001841, Znf_RING IPR013083, Znf_RING/FYVE/PHD |
PANTHERi | PTHR24103:SF587, PTHR24103:SF587, 1 hit |
Pfami | View protein in Pfam PF13765, PRY, 1 hit PF11002, RDM, 1 hit PF00622, SPRY, 1 hit |
PRINTSi | PR01407, BUTYPHLNCDUF |
SMARTi | View protein in SMART SM00589, PRY, 1 hit SM00449, SPRY, 1 hit |
SUPFAMi | SSF49899, SSF49899, 1 hit |
PROSITEi | View protein in PROSITE PS50188, B302_SPRY, 1 hit PS50089, ZF_RING_2, 1 hit |
i Sequence
Sequence statusi: Complete.
O75677-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MKRLSLVTTN RLSPHGNFLP LCTFPLAVDM AALFQEASSC PVCSDYLEKP
60 70 80 90 100
MSLECGCAVC FKCINSLQKE PHGEDLLCCC CSMVSQKNKI RPSWQLERLA
110 120 130 140 150
SHIKELEPKL KKILQMNPRM RKFQVDMTLD ADTANNFLLI SDDLRSVRSG
160 170 180 190 200
CITQNRQDLA ERFDVSICIL GSPRFTCGRH YWEVDVGTST EWDLGVCRES
210 220 230 240 250
VHRKGRIHLT TERGFWTVSL RDGSRLSAST VPLTFLFVDR KLQRVGIFLD
260 270 280 290 300
MGMQNVSFFD AEGGSHVYTF RSVSAEEPLH LFFAPPSPPN GDKSVLSICP
310
VINPGTTDAP VHPGEAK
Sequence cautioni
The sequence CAA09043 differs from that shown. Reason: Erroneous initiation.Curated
The sequence CAA09044 differs from that shown. Reason: Erroneous initiation.Curated
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 289 | P → S in CAG30448 (PubMed:15461802).Curated | 1 |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_039572 | 94 | W → R. Corresponds to variant dbSNP:rs16987627Ensembl. | 1 | |
Natural variantiVAR_020115 | 127 | M → T3 PublicationsCorresponds to variant dbSNP:rs3804076Ensembl. | 1 | |
Natural variantiVAR_039573 | 139 | Missing 3 Publications | 1 | |
Natural variantiVAR_080854 | 148 – 317 | Missing Increased cell proliferation. 1 PublicationCorresponds to variant dbSNP:rs16987628Add BLAST | 170 | |
Natural variantiVAR_080855 | 243 – 317 | Missing 1 PublicationCorresponds to variant dbSNP:rs12484086Add BLAST | 75 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AJ010228 mRNA Translation: CAA09043.1 Different initiation. AJ010229 mRNA Translation: CAA09044.1 Different initiation. CR456562 mRNA Translation: CAG30448.1 BC104768 mRNA No translation available. |
CCDSi | CCDS13857.2 |
RefSeqi | NP_066306.2, NM_021026.2 XP_011528600.1, XM_011530298.2 XP_011528601.1, XM_011530299.2 XP_016884389.1, XM_017028900.1 XP_016884390.1, XM_017028901.1 XP_016884391.1, XM_017028902.1 |
Genome annotation databases
Ensembli | ENST00000354373; ENSP00000346342; ENSG00000128250 |
GeneIDi | 5988 |
KEGGi | hsa:5988 |
UCSCi | uc003afn.3, human |
Keywords - Coding sequence diversityi
PolymorphismSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AJ010228 mRNA Translation: CAA09043.1 Different initiation. AJ010229 mRNA Translation: CAA09044.1 Different initiation. CR456562 mRNA Translation: CAG30448.1 BC104768 mRNA No translation available. |
CCDSi | CCDS13857.2 |
RefSeqi | NP_066306.2, NM_021026.2 XP_011528600.1, XM_011530298.2 XP_011528601.1, XM_011530299.2 XP_016884389.1, XM_017028900.1 XP_016884390.1, XM_017028901.1 XP_016884391.1, XM_017028902.1 |
3D structure databases
SMRi | O75677 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 111920, 1 interactor |
IntActi | O75677, 1 interactor |
STRINGi | 9606.ENSP00000346342 |
PTM databases
iPTMneti | O75677 |
PhosphoSitePlusi | O75677 |
Polymorphism and mutation databases
BioMutai | RFPL1 |
Proteomic databases
jPOSTi | O75677 |
PaxDbi | O75677 |
PeptideAtlasi | O75677 |
PRIDEi | O75677 |
ProteomicsDBi | 50151 |
Protocols and materials databases
Antibodypediai | 24513, 69 antibodies |
DNASUi | 5988 |
Genome annotation databases
Ensembli | ENST00000354373; ENSP00000346342; ENSG00000128250 |
GeneIDi | 5988 |
KEGGi | hsa:5988 |
UCSCi | uc003afn.3, human |
Organism-specific databases
CTDi | 5988 |
DisGeNETi | 5988 |
EuPathDBi | HostDB:ENSG00000128250.5 |
GeneCardsi | RFPL1 |
HGNCi | HGNC:9977, RFPL1 |
HPAi | ENSG00000128250, Tissue enriched (brain) |
MIMi | 605968, gene |
neXtProti | NX_O75677 |
OpenTargetsi | ENSG00000128250 |
PharmGKBi | PA34346 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG2177, Eukaryota |
GeneTreei | ENSGT00940000163408 |
HOGENOMi | CLU_013137_7_1_1 |
InParanoidi | O75677 |
OMAi | CDIHYES |
OrthoDBi | 1139938at2759 |
PhylomeDBi | O75677 |
TreeFami | TF317532 |
Enzyme and pathway databases
PathwayCommonsi | O75677 |
Miscellaneous databases
BioGRID-ORCSi | 5988, 3 hits in 819 CRISPR screens |
ChiTaRSi | RFPL1, human |
GenomeRNAii | 5988 |
Pharosi | O75677, Tdark |
PROi | PR:O75677 |
RNActi | O75677, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000128250, Expressed in prefrontal cortex and 60 other tissues |
Genevisiblei | O75677, HS |
Family and domain databases
CDDi | cd15821, SPRY_PRY_RFPL, 1 hit |
Gene3Di | 2.60.120.920, 1 hit 3.30.40.10, 1 hit |
InterProi | View protein in InterPro IPR001870, B30.2/SPRY IPR043136, B30.2/SPRY_sf IPR003879, Butyrophylin_SPRY IPR013320, ConA-like_dom_sf IPR006574, PRY IPR022723, RDM_domain_RFPL IPR038908, RFPL IPR037960, SPRY/PRY_RFPL IPR003877, SPRY_dom IPR001841, Znf_RING IPR013083, Znf_RING/FYVE/PHD |
PANTHERi | PTHR24103:SF587, PTHR24103:SF587, 1 hit |
Pfami | View protein in Pfam PF13765, PRY, 1 hit PF11002, RDM, 1 hit PF00622, SPRY, 1 hit |
PRINTSi | PR01407, BUTYPHLNCDUF |
SMARTi | View protein in SMART SM00589, PRY, 1 hit SM00449, SPRY, 1 hit |
SUPFAMi | SSF49899, SSF49899, 1 hit |
PROSITEi | View protein in PROSITE PS50188, B302_SPRY, 1 hit PS50089, ZF_RING_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | RFPL1_HUMAN | |
Accessioni | O75677Primary (citable) accession number: O75677 Secondary accession number(s): Q6IC06, Q9UJ97 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | September 26, 2001 |
Last sequence update: | March 15, 2005 | |
Last modified: | December 2, 2020 | |
This is version 152 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Human polymorphisms and disease mutations
Index of human polymorphisms and disease mutations - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - Human chromosome 22
Human chromosome 22: entries, gene names and cross-references to MIM - Human entries with polymorphisms or disease mutations
List of human entries with polymorphisms or disease mutations