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UniProtKB - O75604 (UBP2_HUMAN)

Protein

Ubiquitin carboxyl-terminal hydrolase 2

Gene

USP2

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score:

Annotation score:5 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the ‘correct annotation’ for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>-Experimental evidence at protein leveli <p>This indicates the type of evidence that supports the existence of the protein. Note that the ‘protein existence’ evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Hydrolase that deubiquitinates polyubiquitinated target proteins such as MDM2, MDM4 and CCND1 (PubMed:17290220, PubMed:19917254, PubMed:19838211). Isoform 1 and isoform 4 possess both ubiquitin-specific peptidase and isopeptidase activities (By similarity). Deubiquitinates MDM2 without reversing MDM2-mediated p53/TP53 ubiquitination and thus indirectly promotes p53/TP53 degradation and limits p53 activity (PubMed:17290220, PubMed:19838211). Has no deubiquitinase activity against p53/TP53 (PubMed:17290220). Prevents MDM2-mediated degradation of MDM4 (PubMed:17290220). Plays a role in the G1/S cell-cycle progression in normal and cancer cells (PubMed:19917254). Regulates the circadian clock by modulating its intrinsic circadian rhythm and its capacity to respond to external cues (By similarity). Associates with clock proteins and deubiquitinates core clock component PER1 but does not affect its overall stability (By similarity). Regulates the nucleocytoplasmic shuttling and nuclear retention of PER1 and its repressive role on the clock transcription factors CLOCK and ARNTL/BMAL1 (By similarity). Plays a role in the regulation of myogenic differentiation of embryonic muscle cells (By similarity).By similarity3 Publications
Isoform 4: Circadian clock output effector that regulates Ca2+ absorption in the small intestine. Probably functions by regulating protein levels of the membrane scaffold protein NHERF4 in a rhythmic manner, and is therefore likely to control Ca2+ membrane permeability mediated by the Ca2+ channel TRPV6 in the intestine.By similarity

<p>This subsection of the <a href="http://www.uniprot.org/help/function_section">Function</a> section describes the catalytic activity of an enzyme, i.e. a chemical reaction that the enzyme catalyzes.<p><a href='/help/catalytic_activity' target='_top'>More...</a></p>Catalytic activityi

  • Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal). EC:3.4.19.12

<p>This subsection of the ‘Function’ section describes regulatory mechanisms for enzymes, transporters or microbial transcription factors, and reports the components which regulate (by activation or inhibition) the reaction.<p><a href='/help/activity_regulation' target='_top'>More...</a></p>Activity regulationi

Cleavage is inhibited by ubiquitin in a dosage-dependent manner. Cleavage is blocked by ubiquitin aldehyde.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the ‘Function’ section is used for enzymes and indicates the residues directly involved in catalysis.<p><a href='/help/act_site' target='_top'>More...</a></p>Active sitei276NucleophilePROSITE-ProRule annotation1
<p>This subsection of the ‘Function’ section indicates at which position the protein binds a given metal ion. The nature of the metal is indicated in the ‘Description’ field.<p><a href='/help/metal' target='_top'>More...</a></p>Metal bindingi425Zinc1 Publication1
Metal bindingi428Zinc1 Publication1
Metal bindingi476Zinc1 Publication1
Metal bindingi479Zinc1 Publication1
Active sitei557Proton acceptorPROSITE-ProRule annotation1

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

  • cyclin binding Source: UniProtKB
  • cysteine-type endopeptidase activity Source: ProtInc
  • identical protein binding Source: Ensembl
  • metal ion binding Source: UniProtKB-KW
  • thiol-dependent ubiquitin-specific protease activity Source: FlyBase
  • thiol-dependent ubiquitinyl hydrolase activity Source: Reactome
  • ubiquitin protein ligase binding Source: UniProtKB
View the complete GO annotation on QuickGO ...

GO - Biological processi

View the complete GO annotation on QuickGO ...

<p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

Molecular functionHydrolase, Protease, Thiol protease
Biological processBiological rhythms, Cell cycle, Myogenesis, Ubl conjugation pathway
LigandMetal-binding, Zinc

Enzyme and pathway databases

Reactome - a knowledgebase of biological pathways and processes

More...Reactomei		R-HSA-5357786 TNFR1-induced proapoptotic signaling
R-HSA-5357905 Regulation of TNFR1 signaling
R-HSA-5357956 TNFR1-induced NFkappaB signaling pathway
R-HSA-5689880 Ub-specific processing proteases
R-HSA-6804757 Regulation of TP53 Degradation

Protein family/group databases

MEROPS protease database

More...MEROPSi		C19.013

MoonDB Database of extreme multifunctional and moonlighting proteins

More...MoonDBi		O75604 Predicted

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
Recommended name:
Ubiquitin carboxyl-terminal hydrolase 2 (EC:3.4.19.12)
Alternative name(s):
41 kDa ubiquitin-specific protease
Deubiquitinating enzyme 2
Ubiquitin thioesterase 2
Ubiquitin-specific-processing protease 2
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: ‘Name’, ‘Synonyms’, ‘Ordered locus names’ and ‘ORF names’.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
Name:USP2
Synonyms:UBP41
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiHomo sapiens (Human)
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the ‘taxonomic identifier’ or ‘taxid’.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri9606 [NCBI]
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section is present for entries that are part of a <a href="http://www.uniprot.org/proteomes">proteome</a>, i.e. of a set of proteins thought to be expressed by organisms whose genomes have been completely sequenced.<p><a href='/help/proteomes_manual' target='_top'>More...</a></p>Proteomesi
  • UP000005640 <p>A UniProt <a href="http://www.uniprot.org/manual/proteomes_manual">proteome</a> can consist of several components. <br></br>The component name refers to the genomic component encoding a set of proteins.<p><a href='/help/proteome_component' target='_top'>More...</a></p> Componenti: Chromosome 11

Organism-specific databases

Eukaryotic Pathogen Database Resources

More...EuPathDBi		HostDB:ENSG00000036672.15

Human Gene Nomenclature Database

More...HGNCi		HGNC:12618 USP2

Online Mendelian Inheritance in Man (OMIM)

More...MIMi		604725 gene

neXtProt; the human protein knowledge platform

More...neXtProti		NX_O75604

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Membrane, Nucleus

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/manual/pathology_and_biotech_section">'Pathology and Biotech'</a> section describes the effect of the experimental mutation of one or more amino acid(s) on the biological properties of the protein.<p><a href='/help/mutagen' target='_top'>More...</a></p>Mutagenesisi276C → A: Loss of enzymatic activity. Increases the steady-state level of CCND1. 1 Publication1
Mutagenesisi549H → A: Loss of enzymatic activity. Increases the steady-state level of MDM2 and MDM4 that leads to attenuation of MDM2-mediated degradation of p53/TP53 and MDM4. Increases the level of p53/TP53 and MDM4 ubiquitin conjugates. 2 Publications1

Organism-specific databases

DisGeNET

More...DisGeNETi		9099

Open Targets

More...OpenTargetsi		ENSG00000036672

The Pharmacogenetics and Pharmacogenomics Knowledge Base

More...PharmGKBi		PA37244

Chemistry databases

ChEMBL database of bioactive drug-like small molecules

More...ChEMBLi		CHEMBL1293227

Polymorphism and mutation databases

BioMuta curated single-nucleotide variation and disease association database

More...BioMutai		USP2

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the ‘PTM / Processing’ section describes the extent of a polypeptide chain in the mature protein following processing.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_00000806161 – 605Ubiquitin carboxyl-terminal hydrolase 2Add BLAST605

Proteomic databases

MaxQB - The MaxQuant DataBase

More...MaxQBi		O75604

PaxDb, a database of protein abundance averages across all three domains of life

More...PaxDbi		O75604

PeptideAtlas

More...PeptideAtlasi		O75604

PRoteomics IDEntifications database

More...PRIDEi		O75604

ProteomicsDB human proteome resource

More...ProteomicsDBi		50112
50113 [O75604-2]
50114 [O75604-3]
50115 [O75604-4]

PTM databases

iPTMnet integrated resource for PTMs in systems biology context

More...iPTMneti		O75604

Comprehensive resource for the study of protein post-translational modifications (PTMs) in human, mouse and rat.

More...PhosphoSitePlusi		O75604

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

<p>This subsection of the ‘Expression’ section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms. By default, the information is derived from experiments at the mRNA level, unless specified ‘at protein level’. <br></br>Examples: <a href="http://www.uniprot.org/uniprot/P92958#expression">P92958</a>, <a href="http://www.uniprot.org/uniprot/Q8TDN4#expression">Q8TDN4</a>, <a href="http://www.uniprot.org/uniprot/O14734#expression">O14734</a><p><a href='/help/tissue_specificity' target='_top'>More...</a></p>Tissue specificityi

Expressed in mesangial cells of the kidney and in different types of glomerulonephritides (at protein level).1 Publication

<p>This subsection of the ‘Expression’ section reports the experimentally proven effects of inducers and repressors (usually chemical compounds or environmental factors) on the level of protein (or mRNA) expression (up-regulation, down-regulation, constitutive expression).<p><a href='/help/induction' target='_top'>More...</a></p>Inductioni

Down-regulated by cisplatin (at protein level).1 Publication

Gene expression databases

Bgee dataBase for Gene Expression Evolution

More...Bgeei		ENSG00000036672 Expressed in 183 organ(s), highest expression level in muscle of leg

CleanEx database of gene expression profiles

More...CleanExi		HS_USP2

ExpressionAtlas, Differential and Baseline Expression

More...ExpressionAtlasi		O75604 baseline and differential

Genevisible search portal to normalized and curated expression data from Genevestigator

More...Genevisiblei		O75604 HS

Organism-specific databases

Human Protein Atlas

More...HPAi		HPA006777
HPA007222

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

<p>This subsection of the <a href="http://www.uniprot.org/help/interaction_section">'Interaction'</a> section provides information about the protein quaternary structure and interaction(s) with other proteins or protein complexes (with the exception of physiological receptor-ligand interactions which are annotated in the <a href="http://www.uniprot.org/help/function_section">'Function'</a> section).<p><a href='/help/subunit_structure' target='_top'>More...</a></p>Subunit structurei

Homooligomer (By similarity). Found in trimeric complex with MDM2 and MDM4 and USP2. Interacts with CCND1; the interaction is direct and promotes its stabilization by antagonizing ubiquitin-dependent degradation. Interacts (via N-terminus and C-terminus) with MDM2. Interacts with MDM4. Interacts with PER1 (By similarity). Interacts with KCNQ1; counteracts the NEDD4L-specific down-regulation of I(Ks) and restore plasma membrane localization of KCNQ1 (PubMed:22024150). Isoform 4: Interacts with PDZD3 and CLTC (By similarity).By similarity4 Publications

<p>This subsection of the '<a href="http://www.uniprot.org/help/interaction_section%27">Interaction</a> section provides information about binary protein-protein interactions. The data presented in this section are a quality-filtered subset of binary interactions automatically derived from the <a href="http://www.ebi.ac.uk/intact/">IntAct database</a>. It is updated on a monthly basis. Each binary interaction is displayed on a separate line.<p><a href='/help/binary_interactions' target='_top'>More...</a></p>Binary interactionsi

GO - Molecular functioni

View the complete GO annotation on QuickGO ...

Protein-protein interaction databases

The Biological General Repository for Interaction Datasets (BioGrid)

More...BioGridi		114553, 109 interactors

CORUM comprehensive resource of mammalian protein complexes

More...CORUMi		O75604

Database of interacting proteins

More...DIPi		DIP-29134N

Protein interaction database and analysis system

More...IntActi		O75604, 116 interactors

Molecular INTeraction database

More...MINTi		O75604

STRING: functional protein association networks

More...STRINGi		9606.ENSP00000260187

Chemistry databases

BindingDB database of measured binding affinities

More...BindingDBi		O75604

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

Secondary structure

1605
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

Select the link destinations:

Protein Data Bank Europe

More...PDBei

Protein Data Bank RCSB

More...RCSB PDBi

Protein Data Bank Japan

More...PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2HD5X-ray1.85A258-605[»]
2IBIX-ray2.20A251-605[»]
3NHEX-ray1.26A258-605[»]
3V6CX-ray1.70A258-605[»]
3V6EX-ray2.10A258-605[»]
5XU8X-ray1.81A258-605[»]
5XVEX-ray1.24A258-605[»]

Protein Model Portal of the PSI-Nature Structural Biology Knowledgebase

More...ProteinModelPortali		O75604

SWISS-MODEL Repository - a database of annotated 3D protein structure models

More...SMRi		O75604

Database of comparative protein structure models

More...ModBasei		Search...

MobiDB: a database of protein disorder and mobility annotations

More...MobiDBi		Search...

Miscellaneous databases

Relative evolutionary importance of amino acids within a protein sequence

More...EvolutionaryTracei		O75604

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/family_and_domains_section">Family and Domains</a> section describes the position and type of a domain, which is defined as a specific combination of secondary structures organized into a characteristic three-dimensional structure or fold.<p><a href='/help/domain' target='_top'>More...</a></p>Domaini267 – 599USPAdd BLAST333

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the ‘Family and Domains’ section describes a region of interest that cannot be described in other subsections.<p><a href='/help/region' target='_top'>More...</a></p>Regioni1 – 200Necessary for interaction with MDM41 PublicationAdd BLAST200
Regioni403 – 503Necessary for interaction with MDM41 PublicationAdd BLAST101

<p>This subsection of the ‘Family and domains’ section provides general information on the biological role of a domain. The term ‘domain’ is intended here in its wide acceptation, it may be a structural domain, a transmembrane region or a functional domain. Several domains are described in this subsection.<p><a href='/help/domain_cc' target='_top'>More...</a></p>Domaini

The different N-terminus extensions of isoform 1 and isoform 4 determine their respective subcellular localization and differentiel effect on myoblast fusion and accumulation of muscle-specific proteins. The different N-terminus extensions of isoform 1 and isoform 4 are not essential for their catalytic activity.By similarity

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Belongs to the peptidase C19 family. USP2 subfamily.Curated

Phylogenomic databases

evolutionary genealogy of genes: Non-supervised Orthologous Groups

More...eggNOGi		KOG1868 Eukaryota
ENOG410XP8T LUCA

Ensembl GeneTree

More...GeneTreei		ENSGT00940000161289

The HOGENOM Database of Homologous Genes from Fully Sequenced Organisms

More...HOGENOMi		HOG000231498

The HOVERGEN Database of Homologous Vertebrate Genes

More...HOVERGENi		HBG011164

InParanoid: Eukaryotic Ortholog Groups

More...InParanoidi		O75604

KEGG Orthology (KO)

More...KOi		K11833

Identification of Orthologs from Complete Genome Data

More...OMAi		CLSNTKP

Database of Orthologous Groups

More...OrthoDBi		1283205at2759

Database for complete collections of gene phylogenies

More...PhylomeDBi		O75604

TreeFam database of animal gene trees

More...TreeFami		TF106277

Family and domain databases

Integrated resource of protein families, domains and functional sites

More...InterProi		View protein in InterPro
IPR038765 Papain_like_cys_pep_sf
IPR001394 Peptidase_C19_UCH
IPR018200 USP_CS
IPR028889 USP_dom

Pfam protein domain database

More...Pfami		View protein in Pfam
PF00443 UCH, 1 hit

Superfamily database of structural and functional annotation

More...SUPFAMi		SSF54001 SSF54001, 1 hit

PROSITE; a protein domain and family database

More...PROSITEi		View protein in PROSITE
PS00972 USP_1, 1 hit
PS00973 USP_2, 1 hit
PS50235 USP_3, 1 hit

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence_length">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>.<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequences (4+)i

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences_section">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

This entry describes 4 <p>This subsection of the ‘Sequence’ section lists the alternative protein sequences (isoforms) that can be generated from the same gene by a single or by the combination of up to four biological events (alternative promoter usage, alternative splicing, alternative initiation and ribosomal frameshifting). Additionally, this section gives relevant information on each alternative protein isoform.<p><a href='/help/alternative_products' target='_top'>More...</a></p> isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 4 described isoforms and 2 potential isoforms that are computationally mapped.Show allAlign All

Isoform 1 (identifier: O75604-1) [UniParc]FASTAAdd to basket
Also known as: USP2a, USP2-69

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MSQLSSTLKR YTESARYTDA HYAKSGYGAY TPSSYGANLA ASLLEKEKLG 
        60         70         80         90        100
FKPVPTSSFL TRPRTYGPSS LLDYDRGRPL LRPDITGGGK RAESQTRGTE 
       110        120        130        140        150
RPLGSGLSGG SGFPYGVTNN CLSYLPINAY DQGVTLTQKL DSQSDLARDF 
       160        170        180        190        200
SSLRTSDSYR IDPRNLGRSP MLARTRKELC TLQGLYQTAS CPEYLVDYLE 
       210        220        230        240        250
NYGRKGSASQ VPSQAPPSRV PEIISPTYRP IGRYTLWETG KGQAPGPSRS 
       260        270        280        290        300
SSPGRDGMNS KSAQGLAGLR NLGNTCFMNS ILQCLSNTRE LRDYCLQRLY 
       310        320        330        340        350
MRDLHHGSNA HTALVEEFAK LIQTIWTSSP NDVVSPSEFK TQIQRYAPRF 
       360        370        380        390        400
VGYNQQDAQE FLRFLLDGLH NEVNRVTLRP KSNPENLDHL PDDEKGRQMW 
       410        420        430        440        450
RKYLEREDSR IGDLFVGQLK SSLTCTDCGY CSTVFDPFWD LSLPIAKRGY 
       460        470        480        490        500
PEVTLMDCMR LFTKEDVLDG DEKPTCCRCR GRKRCIKKFS IQRFPKILVL 
       510        520        530        540        550
HLKRFSESRI RTSKLTTFVN FPLRDLDLRE FASENTNHAV YNLYAVSNHS 
       560        570        580        590        600
GTTMGGHYTA YCRSPGTGEW HTFNDSSVTP MSSSQVRTSD AYLLFYELAS 

PPSRM
Length:605
Mass (Da):68,072
Last modified:March 27, 2002 - v2
<p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:iAFF4DA9344D21812
GO
Isoform 2 (identifier: O75604-2) [UniParc]FASTAAdd to basket
Also known as: USP2b

The sequence of this isoform differs from the canonical sequence as follows:
     1-252: Missing.
     253-258: PGRDGM → MLNKAK

Note: No experimental confirmation available.
Show »
Length:353
Mass (Da):40,638
Checksum:i7CDF2F9A300B864F
GO
Isoform 3 (identifier: O75604-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-258: MSQLSSTLKR...RSSSPGRDGM → MLVPGSTRPYSKKRQ

Show »
Length:362
Mass (Da):41,682
Checksum:i5C93CA5F03D43293
GO
Isoform 4 (identifier: O75604-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-258: MSQLSSTLKR...RSSSPGRDGM → MRTSYTVTLP...FVGLLLNKAK

Show »
Length:396
Mass (Da):45,241
Checksum:i2A5B0403419F0655
GO

<p>In eukaryotic reference proteomes, unreviewed entries that are likely to belong to the same gene are computationally mapped, based on gene identifiers from Ensembl, EnsemblGenomes and model organism databases.<p><a href='/help/gene_centric_isoform_mapping' target='_top'>More...</a></p>Computationally mapped potential isoform sequencesi

There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
E9PJ34E9PJ34_HUMAN
Ubiquitin carboxyl-terminal hydrola...
USP2
137Annotation score:

Annotation score:1 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the ‘correct annotation’ for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
E9PSH6E9PSH6_HUMAN
Ubiquitin carboxyl-terminal hydrola...
USP2
167Annotation score:

Annotation score:1 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the ‘correct annotation’ for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the ‘Sequence’ section reports difference(s) between the canonical sequence (displayed by default in the entry) and the different sequence submissions merged in the entry. These various submissions may originate from different sequencing projects, different types of experiments, or different biological samples. Sequence conflicts are usually of unknown origin.<p><a href='/help/conflict' target='_top'>More...</a></p>Sequence conflicti421S → G in BAB71388 (PubMed:14702039).Curated1
Sequence conflicti501H → R in BAB71388 (PubMed:14702039).Curated1
Sequence conflicti594L → H in AAC28392 (Ref. 1) Curated1
Sequence conflicti602 – 605PSRM → TSPI in AAC28392 (Ref. 1) Curated4
Isoform 3 (identifier: O75604-3)
Sequence conflicti10Y → S in AAH41366 (PubMed:15489334).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the ‘Sequence’ section describes natural variant(s) of the protein sequence.<p><a href='/help/variant' target='_top'>More...</a></p>Natural variantiVAR_051519174R → Q. Corresponds to variant dbSNP:rs33929148Ensembl.1
Natural variantiVAR_051520383N → S. Corresponds to variant dbSNP:rs45533837Ensembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the ‘Sequence’ section describes the sequence of naturally occurring alternative protein isoform(s). The changes in the amino acid sequence may be due to alternative splicing, alternative promoter usage, alternative initiation, or ribosomal frameshifting. The information stored in this subsection is used to automatically construct alternative protein sequence(s) for display.<p><a href='/help/var_seq' target='_top'>More...</a></p>Alternative sequenceiVSP_0395591 – 258MSQLS…GRDGM → MLVPGSTRPYSKKRQ in isoform 3. 1 PublicationAdd BLAST258
Alternative sequenceiVSP_0395601 – 258MSQLS…GRDGM → MRTSYTVTLPEDPPAAPFPA LAKELRPRSPLSPSLLLSTF VGLLLNKAK in isoform 4. 2 PublicationsAdd BLAST258
Alternative sequenceiVSP_0052561 – 252Missing in isoform 2. 1 PublicationAdd BLAST252
Alternative sequenceiVSP_005257253 – 258PGRDGM → MLNKAK in isoform 2. 1 Publication6

Sequence databases

Select the link destinations:

EMBL nucleotide sequence database

More...EMBLi

GenBank nucleotide sequence database

More...GenBanki

DNA Data Bank of Japan; a nucleotide sequence database

More...DDBJi
Links Updated
AF079564 mRNA Translation: AAC28392.1
AF440755 mRNA Translation: AAN65363.1
AK057225 mRNA Translation: BAB71388.1
AK292255 mRNA Translation: BAF84944.1
EF445044 Genomic DNA Translation: ACA06096.1
EF445044 Genomic DNA Translation: ACA06097.1
AP003396 Genomic DNA No translation available.
CH471065 Genomic DNA Translation: EAW67484.1
CH471065 Genomic DNA Translation: EAW67485.1
CH471065 Genomic DNA Translation: EAW67486.1
BC002854 mRNA Translation: AAH02854.1
BC002955 mRNA Translation: AAH02955.1
BC041366 mRNA Translation: AAH41366.1

The Consensus CDS (CCDS) project

More...CCDSi		CCDS58189.1 [O75604-3]
CCDS8422.1 [O75604-1]
CCDS8423.1 [O75604-4]

NCBI Reference Sequences

More...RefSeqi		NP_001230688.1, NM_001243759.1 [O75604-3]
NP_004196.4, NM_004205.4 [O75604-1]
NP_741994.1, NM_171997.2 [O75604-4]
XP_005271778.1, XM_005271721.4 [O75604-1]
XP_005271779.1, XM_005271722.2 [O75604-1]

UniGene gene-oriented nucleotide sequence clusters

More...UniGenei		Hs.524085

Genome annotation databases

Ensembl eukaryotic genome annotation project

More...Ensembli		ENST00000260187; ENSP00000260187; ENSG00000036672 [O75604-1]
ENST00000455332; ENSP00000407842; ENSG00000036672 [O75604-3]
ENST00000525735; ENSP00000436952; ENSG00000036672 [O75604-4]

Database of genes from NCBI RefSeq genomes

More...GeneIDi		9099

KEGG: Kyoto Encyclopedia of Genes and Genomes

More...KEGGi		hsa:9099

UCSC genome browser

More...UCSCi		uc001pwl.5 human [O75604-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

<p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF079564 mRNA Translation: AAC28392.1
AF440755 mRNA Translation: AAN65363.1
AK057225 mRNA Translation: BAB71388.1
AK292255 mRNA Translation: BAF84944.1
EF445044 Genomic DNA Translation: ACA06096.1
EF445044 Genomic DNA Translation: ACA06097.1
AP003396 Genomic DNA No translation available.
CH471065 Genomic DNA Translation: EAW67484.1
CH471065 Genomic DNA Translation: EAW67485.1
CH471065 Genomic DNA Translation: EAW67486.1
BC002854 mRNA Translation: AAH02854.1
BC002955 mRNA Translation: AAH02955.1
BC041366 mRNA Translation: AAH41366.1
CCDSiCCDS58189.1 [O75604-3]
CCDS8422.1 [O75604-1]
CCDS8423.1 [O75604-4]
RefSeqiNP_001230688.1, NM_001243759.1 [O75604-3]
NP_004196.4, NM_004205.4 [O75604-1]
NP_741994.1, NM_171997.2 [O75604-4]
XP_005271778.1, XM_005271721.4 [O75604-1]
XP_005271779.1, XM_005271722.2 [O75604-1]
UniGeneiHs.524085

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2HD5X-ray1.85A258-605[»]
2IBIX-ray2.20A251-605[»]
3NHEX-ray1.26A258-605[»]
3V6CX-ray1.70A258-605[»]
3V6EX-ray2.10A258-605[»]
5XU8X-ray1.81A258-605[»]
5XVEX-ray1.24A258-605[»]
ProteinModelPortaliO75604
SMRiO75604
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi114553, 109 interactors
CORUMiO75604
DIPiDIP-29134N
IntActiO75604, 116 interactors
MINTiO75604
STRINGi9606.ENSP00000260187

Chemistry databases

BindingDBiO75604
ChEMBLiCHEMBL1293227

Protein family/group databases

MEROPSiC19.013
MoonDBiO75604 Predicted

PTM databases

iPTMnetiO75604
PhosphoSitePlusiO75604

Polymorphism and mutation databases

BioMutaiUSP2

Proteomic databases

MaxQBiO75604
PaxDbiO75604
PeptideAtlasiO75604
PRIDEiO75604
ProteomicsDBi50112
50113 [O75604-2]
50114 [O75604-3]
50115 [O75604-4]

Protocols and materials databases

The DNASU plasmid repository

More...DNASUi		9099
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000260187; ENSP00000260187; ENSG00000036672 [O75604-1]
ENST00000455332; ENSP00000407842; ENSG00000036672 [O75604-3]
ENST00000525735; ENSP00000436952; ENSG00000036672 [O75604-4]
GeneIDi9099
KEGGihsa:9099
UCSCiuc001pwl.5 human [O75604-1]

Organism-specific databases

Comparative Toxicogenomics Database

More...CTDi		9099
DisGeNETi9099
EuPathDBiHostDB:ENSG00000036672.15

GeneCards: human genes, protein and diseases

More...GeneCardsi		USP2
HGNCiHGNC:12618 USP2
HPAiHPA006777
HPA007222
MIMi604725 gene
neXtProtiNX_O75604
OpenTargetsiENSG00000036672
PharmGKBiPA37244

GenAtlas: human gene database

More...GenAtlasi		Search...

Phylogenomic databases

eggNOGiKOG1868 Eukaryota
ENOG410XP8T LUCA
GeneTreeiENSGT00940000161289
HOGENOMiHOG000231498
HOVERGENiHBG011164
InParanoidiO75604
KOiK11833
OMAiCLSNTKP
OrthoDBi1283205at2759
PhylomeDBiO75604
TreeFamiTF106277

Enzyme and pathway databases

ReactomeiR-HSA-5357786 TNFR1-induced proapoptotic signaling
R-HSA-5357905 Regulation of TNFR1 signaling
R-HSA-5357956 TNFR1-induced NFkappaB signaling pathway
R-HSA-5689880 Ub-specific processing proteases
R-HSA-6804757 Regulation of TP53 Degradation

Miscellaneous databases

ChiTaRS: a database of human, mouse and fruit fly chimeric transcripts and RNA-sequencing data

More...ChiTaRSi		USP2 human
EvolutionaryTraceiO75604

The Gene Wiki collection of pages on human genes and proteins

More...GeneWikii		USP2

Database of phenotypes from RNA interference screens in Drosophila and Homo sapiens

More...GenomeRNAii		9099

Protein Ontology

More...PROi		PR:O75604

The Stanford Online Universal Resource for Clones and ESTs

More...SOURCEi		Search...

Gene expression databases

BgeeiENSG00000036672 Expressed in 183 organ(s), highest expression level in muscle of leg
CleanExiHS_USP2
ExpressionAtlasiO75604 baseline and differential
GenevisibleiO75604 HS

Family and domain databases

InterProiView protein in InterPro
IPR038765 Papain_like_cys_pep_sf
IPR001394 Peptidase_C19_UCH
IPR018200 USP_CS
IPR028889 USP_dom
PfamiView protein in Pfam
PF00443 UCH, 1 hit
SUPFAMiSSF54001 SSF54001, 1 hit
PROSITEiView protein in PROSITE
PS00972 USP_1, 1 hit
PS00973 USP_2, 1 hit
PS50235 USP_3, 1 hit

ProtoNet; Automatic hierarchical classification of proteins

More...ProtoNeti		Search...

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This subsection of the ‘Entry information’ section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiUBP2_HUMAN
<p>This subsection of the ‘Entry information’ section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called ‘Primary (citable) accession number’.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: O75604
Secondary accession number(s): B0YJB8
, E9PPM2, Q8IUM2, Q8IW04, Q96MB9, Q9BQ21
<p>This subsection of the ‘Entry information’ section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification (‘Last modified’). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: March 27, 2002
Last modified: January 16, 2019
This is version 176 of the entry and version 2 of the sequence. See complete history.
<p>This subsection of the ‘Entry information’ section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families
  3. Human chromosome 11
    Human chromosome 11: entries, gene names and cross-references to MIM
  4. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  5. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  6. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  7. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
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