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Protein

Mediator of RNA polymerase II transcription subunit 6

Gene

MED6

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.1 Publication

GO - Molecular functioni

  • RNA polymerase II transcription coactivator activity involved in preinitiation complex assembly Source: GO_Central
  • transcription coactivator activity Source: MGI
  • transcription factor binding Source: UniProtKB

GO - Biological processi

Keywordsi

Molecular functionActivator
Biological processTranscription, Transcription regulation

Enzyme and pathway databases

ReactomeiR-HSA-1989781 PPARA activates gene expression
R-HSA-212436 Generic Transcription Pathway
R-HSA-381340 Transcriptional regulation of white adipocyte differentiation

Names & Taxonomyi

Protein namesi
Recommended name:
Mediator of RNA polymerase II transcription subunit 6
Alternative name(s):
Activator-recruited cofactor 33 kDa component
Short name:
ARC33
Mediator complex subunit 6
Short name:
hMed6
Renal carcinoma antigen NY-REN-28
Gene namesi
Name:MED6
Synonyms:ARC33
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 14

Organism-specific databases

EuPathDBiHostDB:ENSG00000133997.11
HGNCiHGNC:19970 MED6
MIMi602984 gene
neXtProtiNX_O75586

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Organism-specific databases

OpenTargetsiENSG00000133997
PharmGKBiPA134868263

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000963881 – 246Mediator of RNA polymerase II transcription subunit 6Add BLAST246

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Cross-linki208Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Modified residuei236N6-acetyllysineCombined sources1
Modified residuei241N6-acetyllysineCombined sources1

Keywords - PTMi

Acetylation, Isopeptide bond, Ubl conjugation

Proteomic databases

EPDiO75586
MaxQBiO75586
PaxDbiO75586
PeptideAtlasiO75586
PRIDEiO75586
ProteomicsDBi50100

PTM databases

iPTMnetiO75586
PhosphoSitePlusiO75586

Expressioni

Gene expression databases

BgeeiENSG00000133997 Expressed in 207 organ(s), highest expression level in intestine
CleanExiHS_MED6
ExpressionAtlasiO75586 baseline and differential
GenevisibleiO75586 HS

Organism-specific databases

HPAiHPA030764
HPA069039

Interactioni

Subunit structurei

Component of the Mediator complex, which is composed of MED1, MED4, MED6, MED7, MED8, MED9, MED10, MED11, MED12, MED13, MED13L, MED14, MED15, MED16, MED17, MED18, MED19, MED20, MED21, MED22, MED23, MED24, MED25, MED26, MED27, MED29, MED30, MED31, CCNC, CDK8 and CDC2L6/CDK11. The MED12, MED13, CCNC and CDK8 subunits form a distinct module termed the CDK8 module. Mediator containing the CDK8 module is less active than Mediator lacking this module in supporting transcriptional activation. Individual preparations of the Mediator complex lacking one or more distinct subunits have been variously termed ARC, CRSP, DRIP, PC2, SMCC and TRAP. Interacts with CTNNB1 and GLI3.9 Publications

Binary interactionsi

GO - Molecular functioni

Protein-protein interaction databases

BioGridi115319, 59 interactors
ComplexPortaliCPX-3227 Core mediator complex
CORUMiO75586
DIPiDIP-31456N
IntActiO75586, 29 interactors
MINTiO75586
STRINGi9606.ENSP00000256379

Structurei

3D structure databases

ProteinModelPortaliO75586
SMRiO75586
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the Mediator complex subunit 6 family.Curated

Phylogenomic databases

eggNOGiKOG3169 Eukaryota
COG5097 LUCA
GeneTreeiENSGT00390000017666
HOGENOMiHOG000035127
HOVERGENiHBG006825
InParanoidiO75586
KOiK15128
PhylomeDBiO75586
TreeFamiTF313577

Family and domain databases

Gene3Di3.10.450.580, 1 hit
InterProiView protein in InterPro
IPR038566 Med6_sf
IPR007018 Mediator_Med6
IPR016820 Mediator_Med6_met/pln
PANTHERiPTHR13104 PTHR13104, 1 hit
PfamiView protein in Pfam
PF04934 Med6, 1 hit
PIRSFiPIRSF023869 Mediator_MED6_meta/pln, 1 hit

Sequences (3+)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 3 described isoforms and 2 potential isoforms that are computationally mapped.Show allAlign All

Isoform 1 (identifier: O75586-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MAAVDIRDNL LGISWVDSSW IPILNSGSVL DYFSERSNPF YDRTCNNEVV
60 70 80 90 100
KMQRLTLEHL NQMVGIEYIL LHAQEPILFI IRKQQRQSPA QVIPLADYYI
110 120 130 140 150
IAGVIYQAPD LGSVINSRVL TAVHGIQSAF DEAMSYCRYH PSKGYWWHFK
160 170 180 190 200
DHEEQDKVRP KAKRKEEPSS IFQRQRVDAL LLDLRQKFPP KFVQLKPGEK
210 220 230 240
PVPVDQTKKE AEPIPETVKP EEKETTKNVQ QTVSAKGPPE KRMRLQ
Length:246
Mass (Da):28,425
Last modified:February 28, 2003 - v2
Checksum:i9B173626F583B48B
GO
Isoform 2 (identifier: O75586-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     119-119: V → VSLFSFYK

Note: No experimental confirmation available.
Show »
Length:253
Mass (Da):29,298
Checksum:i3D89AD48C96C3CD7
GO
Isoform 3 (identifier: O75586-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     156-246: DKVRPKAKRK...GPPEKRMRLQ → AKAWRKACSSGSNKERGRTYTRNCKT

Note: No experimental confirmation available.
Show »
Length:181
Mass (Da):20,859
Checksum:i672B435005F9E984
GO

Computationally mapped potential isoform sequencesi

There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
A0A087WYL7A0A087WYL7_HUMAN
Mediator of RNA polymerase II trans...
MED6
257Annotation score:
G3V4A5G3V4A5_HUMAN
Mediator of RNA polymerase II trans...
MED6
249Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti151D → V in AAC26869 (PubMed:9734358).Curated1
Sequence conflicti156D → G in AAB84363 (PubMed:10508479).Curated1
Sequence conflicti203P → S in BAD97066 (Ref. 5) Curated1
Sequence conflicti224E → G in BAD96307 (Ref. 5) Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_054589119V → VSLFSFYK in isoform 2. 1 Publication1
Alternative sequenceiVSP_054590156 – 246DKVRP…RMRLQ → AKAWRKACSSGSNKERGRTY TRNCKT in isoform 3. 1 PublicationAdd BLAST91

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF074723 mRNA Translation: AAC26869.1
BT006831 mRNA Translation: AAP35477.1
CR541697 mRNA Translation: CAG46498.1
AK300460 mRNA Translation: BAG62179.1
AK304361 mRNA Translation: BAG65202.1
AK222587 mRNA Translation: BAD96307.1
AK223346 mRNA Translation: BAD97066.1
AL357153 Genomic DNA No translation available.
BC004106 mRNA Translation: AAH04106.1
U78082 mRNA Translation: AAB84363.1
AF155104 mRNA Translation: AAD42870.1
CCDSiCCDS61483.1 [O75586-3]
CCDS61484.1 [O75586-2]
CCDS9805.1 [O75586-1]
RefSeqiNP_001271138.1, NM_001284209.1 [O75586-2]
NP_001271139.1, NM_001284210.1 [O75586-3]
NP_001271140.1, NM_001284211.1
NP_005457.2, NM_005466.3 [O75586-1]
UniGeneiHs.497353

Genome annotation databases

EnsembliENST00000256379; ENSP00000256379; ENSG00000133997 [O75586-1]
ENST00000430055; ENSP00000413343; ENSG00000133997 [O75586-2]
ENST00000440435; ENSP00000394502; ENSG00000133997 [O75586-3]
GeneIDi10001
KEGGihsa:10001
UCSCiuc001xmf.5 human [O75586-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF074723 mRNA Translation: AAC26869.1
BT006831 mRNA Translation: AAP35477.1
CR541697 mRNA Translation: CAG46498.1
AK300460 mRNA Translation: BAG62179.1
AK304361 mRNA Translation: BAG65202.1
AK222587 mRNA Translation: BAD96307.1
AK223346 mRNA Translation: BAD97066.1
AL357153 Genomic DNA No translation available.
BC004106 mRNA Translation: AAH04106.1
U78082 mRNA Translation: AAB84363.1
AF155104 mRNA Translation: AAD42870.1
CCDSiCCDS61483.1 [O75586-3]
CCDS61484.1 [O75586-2]
CCDS9805.1 [O75586-1]
RefSeqiNP_001271138.1, NM_001284209.1 [O75586-2]
NP_001271139.1, NM_001284210.1 [O75586-3]
NP_001271140.1, NM_001284211.1
NP_005457.2, NM_005466.3 [O75586-1]
UniGeneiHs.497353

3D structure databases

ProteinModelPortaliO75586
SMRiO75586
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi115319, 59 interactors
ComplexPortaliCPX-3227 Core mediator complex
CORUMiO75586
DIPiDIP-31456N
IntActiO75586, 29 interactors
MINTiO75586
STRINGi9606.ENSP00000256379

PTM databases

iPTMnetiO75586
PhosphoSitePlusiO75586

Proteomic databases

EPDiO75586
MaxQBiO75586
PaxDbiO75586
PeptideAtlasiO75586
PRIDEiO75586
ProteomicsDBi50100

Protocols and materials databases

DNASUi10001
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000256379; ENSP00000256379; ENSG00000133997 [O75586-1]
ENST00000430055; ENSP00000413343; ENSG00000133997 [O75586-2]
ENST00000440435; ENSP00000394502; ENSG00000133997 [O75586-3]
GeneIDi10001
KEGGihsa:10001
UCSCiuc001xmf.5 human [O75586-1]

Organism-specific databases

CTDi10001
EuPathDBiHostDB:ENSG00000133997.11
GeneCardsiMED6
HGNCiHGNC:19970 MED6
HPAiHPA030764
HPA069039
MIMi602984 gene
neXtProtiNX_O75586
OpenTargetsiENSG00000133997
PharmGKBiPA134868263
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG3169 Eukaryota
COG5097 LUCA
GeneTreeiENSGT00390000017666
HOGENOMiHOG000035127
HOVERGENiHBG006825
InParanoidiO75586
KOiK15128
PhylomeDBiO75586
TreeFamiTF313577

Enzyme and pathway databases

ReactomeiR-HSA-1989781 PPARA activates gene expression
R-HSA-212436 Generic Transcription Pathway
R-HSA-381340 Transcriptional regulation of white adipocyte differentiation

Miscellaneous databases

ChiTaRSiMED6 human
GeneWikiiMED6
GenomeRNAii10001
PROiPR:O75586
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000133997 Expressed in 207 organ(s), highest expression level in intestine
CleanExiHS_MED6
ExpressionAtlasiO75586 baseline and differential
GenevisibleiO75586 HS

Family and domain databases

Gene3Di3.10.450.580, 1 hit
InterProiView protein in InterPro
IPR038566 Med6_sf
IPR007018 Mediator_Med6
IPR016820 Mediator_Med6_met/pln
PANTHERiPTHR13104 PTHR13104, 1 hit
PfamiView protein in Pfam
PF04934 Med6, 1 hit
PIRSFiPIRSF023869 Mediator_MED6_meta/pln, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiMED6_HUMAN
AccessioniPrimary (citable) accession number: O75586
Secondary accession number(s): B4DU17
, B4E2P0, O15401, Q53FE3, Q53HJ3, Q6FHQ4, Q9BTH1, Q9UHL1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 28, 2003
Last sequence update: February 28, 2003
Last modified: November 7, 2018
This is version 158 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. Human chromosome 14
    Human chromosome 14: entries, gene names and cross-references to MIM
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Main funding by: National Institutes of Health

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