UniProtKB - O75563 (SKAP2_HUMAN)
Src kinase-associated phosphoprotein 2
SKAP2
Functioni
May be involved in B-cell and macrophage adhesion processes. In B-cells, may act by coupling the B-cell receptor (BCR) to integrin activation. May play a role in src signaling pathway.
2 PublicationsGO - Biological processi
- B cell activation Source: UniProtKB-KW
- negative regulation of cell population proliferation Source: Ensembl
- protein-containing complex assembly Source: ProtInc
- signal transduction Source: ProtInc
Keywordsi
Biological process | B-cell activation |
Enzyme and pathway databases
PathwayCommonsi | O75563 |
Reactomei | R-HSA-391160, Signal regulatory protein family interactions |
SignaLinki | O75563 |
Names & Taxonomyi
Protein namesi | Recommended name: Src kinase-associated phosphoprotein 2Alternative name(s): Pyk2/RAFTK-associated protein Retinoic acid-induced protein 70 SKAP55 homolog Short name: SKAP-55HOM Short name: SKAP-HOM Src family-associated phosphoprotein 2 Src kinase-associated phosphoprotein 55-related protein Src-associated adapter protein with PH and SH3 domains |
Gene namesi | Name:SKAP2 Synonyms:PRAP, RA70, SAPS, SCAP2, SKAP55R |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:15687, SKAP2 |
MIMi | 605215, gene |
neXtProti | NX_O75563 |
VEuPathDBi | HostDB:ENSG00000005020 |
Subcellular locationi
Cytoplasm and Cytosol
- Cytoplasm 1 Publication
Cytosol
- cytosol Source: HPA
Nucleus
- nucleoplasm Source: HPA
Plasma Membrane
- plasma membrane Source: MGI
Other locations
- cytoplasm Source: GO_Central
Keywords - Cellular componenti
CytoplasmPathology & Biotechi
Organism-specific databases
DisGeNETi | 8935 |
OpenTargetsi | ENSG00000005020 |
PharmGKBi | PA162403393 |
Miscellaneous databases
Pharosi | O75563, Tbio |
Genetic variation databases
BioMutai | SKAP2 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000270179 | 1 – 359 | Src kinase-associated phosphoprotein 2Add BLAST | 359 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 5 | PhosphoserineCombined sources | 1 | |
Modified residuei | 6 | PhosphoserineCombined sources | 1 | |
Modified residuei | 9 | PhosphoserineBy similarity | 1 | |
Modified residuei | 75 | Phosphotyrosine1 Publication | 1 | |
Modified residuei | 87 | PhosphoserineBy similarity | 1 | |
Modified residuei | 90 | PhosphoserineBy similarity | 1 | |
Modified residuei | 151 | PhosphotyrosineBy similarity | 1 | |
Modified residuei | 197 | PhosphotyrosineCombined sources | 1 | |
Modified residuei | 223 | PhosphoserineBy similarity | 1 | |
Modified residuei | 261 | PhosphotyrosineBy similarity | 1 | |
Modified residuei | 283 | PhosphoserineBy similarity | 1 | |
Modified residuei | 286 | PhosphoserineBy similarity | 1 |
Post-translational modificationi
Keywords - PTMi
PhosphoproteinProteomic databases
EPDi | O75563 |
MassIVEi | O75563 |
MaxQBi | O75563 |
PaxDbi | O75563 |
PeptideAtlasi | O75563 |
PRIDEi | O75563 |
ProteomicsDBi | 50086 |
2D gel databases
OGPi | O75563 |
PTM databases
iPTMneti | O75563 |
PhosphoSitePlusi | O75563 |
Expressioni
Tissue specificityi
Inductioni
Gene expression databases
Bgeei | ENSG00000005020, Expressed in forebrain and 256 other tissues |
ExpressionAtlasi | O75563, baseline and differential |
Genevisiblei | O75563, HS |
Organism-specific databases
HPAi | ENSG00000005020, Low tissue specificity |
Interactioni
Subunit structurei
Homodimer (By similarity).
Interacts with PTPNS1.
Part of a complex consisting of SKAP2, FYB1 and PTPNS1.
Part of a complex consisting of SKAP2, FYB1 and LILRB3. May interact with actin (By similarity).
Interacts with FYB1, which is required for SKAP2 protein stability.
Interacts with LAT, GRB2, PTK2B and PRAM1. May interact with FYN, HCK and LYN.
Interacts with FASLG.
By similarity7 PublicationsBinary interactionsi
O75563
With | #Exp. | IntAct |
---|---|---|
PTPN22 [Q9Y2R2] | 3 | EBI-2483161,EBI-1211241 |
Protein-protein interaction databases
BioGRIDi | 114448, 43 interactors |
IntActi | O75563, 12 interactors |
STRINGi | 9606.ENSP00000005587 |
Miscellaneous databases
RNActi | O75563, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 116 – 219 | PHPROSITE-ProRule annotationAdd BLAST | 104 | |
Domaini | 297 – 358 | SH3PROSITE-ProRule annotationAdd BLAST | 62 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 14 – 64 | HomodimerizationBy similarityAdd BLAST | 51 | |
Regioni | 66 – 88 | DisorderedSequence analysisAdd BLAST | 23 | |
Regioni | 264 – 293 | DisorderedSequence analysisAdd BLAST | 30 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 273 – 292 | Basic and acidic residuesSequence analysisAdd BLAST | 20 |
Domaini
Sequence similaritiesi
Keywords - Domaini
SH3 domainPhylogenomic databases
eggNOGi | ENOG502QVFD, Eukaryota |
GeneTreei | ENSGT00390000017856 |
HOGENOMi | CLU_062032_0_0_1 |
InParanoidi | O75563 |
OMAi | KQGFPQD |
OrthoDBi | 767446at2759 |
PhylomeDBi | O75563 |
TreeFami | TF331055 |
Family and domain databases
Gene3Di | 2.30.29.30, 1 hit |
InterProi | View protein in InterPro IPR011993, PH-like_dom_sf IPR001849, PH_domain IPR036028, SH3-like_dom_sf IPR001452, SH3_domain IPR037781, SKAP_fam |
PANTHERi | PTHR15129, PTHR15129, 1 hit |
Pfami | View protein in Pfam PF00169, PH, 1 hit PF00018, SH3_1, 1 hit |
PRINTSi | PR00452, SH3DOMAIN |
SMARTi | View protein in SMART SM00233, PH, 1 hit SM00326, SH3, 1 hit |
SUPFAMi | SSF50044, SSF50044, 1 hit |
PROSITEi | View protein in PROSITE PS50003, PH_DOMAIN, 1 hit PS50002, SH3, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
10 20 30 40 50
MPNPSSTSSP YPLPEEIRNL LADVETFVAD ILKGENLSKK AKEKRESLIK
60 70 80 90 100
KIKDVKSIYL QEFQDKGDAE DGEEYDDPFA GPPDTISLAS ERYDKDDEAP
110 120 130 140 150
SDGAQFPPIA AQDLPFVLKA GYLEKRRKDH SFLGFEWQKR WCALSKTVFY
160 170 180 190 200
YYGSDKDKQQ KGEFAIDGYS VRMNNTLRKD GKKDCCFEIS APDKRIYQFT
210 220 230 240 250
AASPKDAEEW VQQLKFVLQD MESDIIPEDY DERGELYDDV DHPLPISNPL
260 270 280 290 300
TSSQPIDDEI YEELPEEEED SAPVKVEEQR KMSQDSVHHT SGDKSTDYAN
310 320 330 340 350
FYQGLWDCTG AFSDELSFKR GDVIYILSKE YNRYGWWVGE MKGAIGLVPK
AYIMEMYDI
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketC9JQ17 | C9JQ17_HUMAN | Src kinase-associated phosphoprotei... | SKAP2 | 126 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 6 | S → C in BAA36194 (PubMed:9837776).Curated | 1 | |
Sequence conflicti | 352 | Y → H in BAD96605 (Ref. 7) Curated | 1 |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_029812 | 202 | A → S3 PublicationsCorresponds to variant dbSNP:rs1129771Ensembl. | 1 | |
Natural variantiVAR_029813 | 253 | S → T. Corresponds to variant dbSNP:rs17154402EnsemblClinVar. | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB014486 mRNA Translation: BAA36194.1 AJ004886 mRNA Translation: CAA06193.1 AF072166 mRNA Translation: AAC39924.1 AF051323 mRNA Translation: AAC99296.1 AK313209 mRNA Translation: BAG36025.1 AK222885 mRNA Translation: BAD96605.1 AC003999 Genomic DNA Translation: AAS02033.1 AC011299 Genomic DNA Translation: AAS07536.1 CH236948 Genomic DNA Translation: EAL24231.1 CH471073 Genomic DNA Translation: EAW93859.1 BC002893 mRNA Translation: AAH02893.1 BC036044 mRNA Translation: AAH36044.1 |
CCDSi | CCDS5400.1 |
RefSeqi | NP_001290397.1, NM_001303468.1 NP_003921.2, NM_003930.4 XP_016868260.1, XM_017012771.1 |
Genome annotation databases
Ensembli | ENST00000345317.7; ENSP00000005587.2; ENSG00000005020.13 |
GeneIDi | 8935 |
KEGGi | hsa:8935 |
MANE-Selecti | ENST00000345317.7; ENSP00000005587.2; NM_003930.5; NP_003921.2 |
UCSCi | uc003syc.4, human |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB014486 mRNA Translation: BAA36194.1 AJ004886 mRNA Translation: CAA06193.1 AF072166 mRNA Translation: AAC39924.1 AF051323 mRNA Translation: AAC99296.1 AK313209 mRNA Translation: BAG36025.1 AK222885 mRNA Translation: BAD96605.1 AC003999 Genomic DNA Translation: AAS02033.1 AC011299 Genomic DNA Translation: AAS07536.1 CH236948 Genomic DNA Translation: EAL24231.1 CH471073 Genomic DNA Translation: EAW93859.1 BC002893 mRNA Translation: AAH02893.1 BC036044 mRNA Translation: AAH36044.1 |
CCDSi | CCDS5400.1 |
RefSeqi | NP_001290397.1, NM_001303468.1 NP_003921.2, NM_003930.4 XP_016868260.1, XM_017012771.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
3OMH | X-ray | 2.90 | E/F/G/H | 71-79 | [»] | |
AlphaFoldDBi | O75563 | |||||
SMRi | O75563 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 114448, 43 interactors |
IntActi | O75563, 12 interactors |
STRINGi | 9606.ENSP00000005587 |
PTM databases
iPTMneti | O75563 |
PhosphoSitePlusi | O75563 |
Genetic variation databases
BioMutai | SKAP2 |
2D gel databases
OGPi | O75563 |
Proteomic databases
EPDi | O75563 |
MassIVEi | O75563 |
MaxQBi | O75563 |
PaxDbi | O75563 |
PeptideAtlasi | O75563 |
PRIDEi | O75563 |
ProteomicsDBi | 50086 |
Protocols and materials databases
Antibodypediai | 25901, 229 antibodies from 33 providers |
DNASUi | 8935 |
Genome annotation databases
Ensembli | ENST00000345317.7; ENSP00000005587.2; ENSG00000005020.13 |
GeneIDi | 8935 |
KEGGi | hsa:8935 |
MANE-Selecti | ENST00000345317.7; ENSP00000005587.2; NM_003930.5; NP_003921.2 |
UCSCi | uc003syc.4, human |
Organism-specific databases
CTDi | 8935 |
DisGeNETi | 8935 |
GeneCardsi | SKAP2 |
HGNCi | HGNC:15687, SKAP2 |
HPAi | ENSG00000005020, Low tissue specificity |
MIMi | 605215, gene |
neXtProti | NX_O75563 |
OpenTargetsi | ENSG00000005020 |
PharmGKBi | PA162403393 |
VEuPathDBi | HostDB:ENSG00000005020 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | ENOG502QVFD, Eukaryota |
GeneTreei | ENSGT00390000017856 |
HOGENOMi | CLU_062032_0_0_1 |
InParanoidi | O75563 |
OMAi | KQGFPQD |
OrthoDBi | 767446at2759 |
PhylomeDBi | O75563 |
TreeFami | TF331055 |
Enzyme and pathway databases
PathwayCommonsi | O75563 |
Reactomei | R-HSA-391160, Signal regulatory protein family interactions |
SignaLinki | O75563 |
Miscellaneous databases
BioGRID-ORCSi | 8935, 77 hits in 1066 CRISPR screens |
ChiTaRSi | SKAP2, human |
GeneWikii | SKAP2 |
GenomeRNAii | 8935 |
Pharosi | O75563, Tbio |
PROi | PR:O75563 |
RNActi | O75563, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000005020, Expressed in forebrain and 256 other tissues |
ExpressionAtlasi | O75563, baseline and differential |
Genevisiblei | O75563, HS |
Family and domain databases
Gene3Di | 2.30.29.30, 1 hit |
InterProi | View protein in InterPro IPR011993, PH-like_dom_sf IPR001849, PH_domain IPR036028, SH3-like_dom_sf IPR001452, SH3_domain IPR037781, SKAP_fam |
PANTHERi | PTHR15129, PTHR15129, 1 hit |
Pfami | View protein in Pfam PF00169, PH, 1 hit PF00018, SH3_1, 1 hit |
PRINTSi | PR00452, SH3DOMAIN |
SMARTi | View protein in SMART SM00233, PH, 1 hit SM00326, SH3, 1 hit |
SUPFAMi | SSF50044, SSF50044, 1 hit |
PROSITEi | View protein in PROSITE PS50003, PH_DOMAIN, 1 hit PS50002, SH3, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | SKAP2_HUMAN | |
Accessioni | O75563Primary (citable) accession number: O75563 Secondary accession number(s): A4D173 Q9UED8 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | January 9, 2007 |
Last sequence update: | November 1, 1998 | |
Last modified: | May 25, 2022 | |
This is version 167 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Human chromosome 7
Human chromosome 7: entries, gene names and cross-references to MIM - Human entries with genetic variants
List of human entries with genetic variants - Human variants curated from literature reports
Index of human variants curated from literature reports - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families