UniProtKB - O75478 (TAD2A_HUMAN)
Transcriptional adapter 2-alpha
TADA2A
Functioni
Component of the ATAC complex, a complex with histone acetyltransferase activity on histones H3 and H4. Required for the function of some acidic activation domains, which activate transcription from a distant site (By similarity).
Binds double-stranded DNA. Binds dinucleosomes, probably at the linker region between neighboring nucleosomes. Plays a role in chromatin remodeling. May promote TP53/p53 'Lys-321' acetylation, leading to reduced TP53 stability and transcriptional activity (PubMed:22644376).
May also promote XRCC6 acetylation thus facilitating cell apoptosis in response to DNA damage (PubMed:22644376).
By similarity2 PublicationsSites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 17 | Zinc 1PROSITE-ProRule annotation | 1 | |
Metal bindingi | 20 | Zinc 1PROSITE-ProRule annotation | 1 | |
Metal bindingi | 31 | Zinc 2PROSITE-ProRule annotation | 1 | |
Metal bindingi | 34 | Zinc 2PROSITE-ProRule annotation | 1 | |
Metal bindingi | 42 | Zinc 1PROSITE-ProRule annotation | 1 | |
Metal bindingi | 45 | Zinc 1PROSITE-ProRule annotation | 1 | |
Metal bindingi | 55 | Zinc 2PROSITE-ProRule annotation | 1 | |
Metal bindingi | 59 | Zinc 2PROSITE-ProRule annotation | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 12 – 69 | ZZ-typePROSITE-ProRule annotationAdd BLAST | 58 | |
DNA bindingi | 426 – 435 | By similarity | 10 |
GO - Molecular functioni
- chromatin binding Source: GO_Central
- DNA binding Source: UniProtKB-KW
- metal ion binding Source: UniProtKB-KW
- transcription coactivator activity Source: GO_Central
GO - Biological processi
- chromatin remodeling Source: GO_Central
- histone acetylation Source: ComplexPortal
- histone H3 acetylation Source: UniProtKB
- histone H3-K14 acetylation Source: ComplexPortal
- positive regulation of histone acetylation Source: GO_Central
- regulation of cell cycle Source: ComplexPortal
- regulation of cell division Source: ComplexPortal
- regulation of embryonic development Source: ComplexPortal
- regulation of histone deacetylation Source: ComplexPortal
- regulation of transcription, DNA-templated Source: ComplexPortal
- regulation of transcription by RNA polymerase II Source: ComplexPortal
- regulation of tubulin deacetylation Source: ComplexPortal
Keywordsi
Molecular function | DNA-binding |
Biological process | Transcription, Transcription regulation |
Ligand | Metal-binding, Zinc |
Enzyme and pathway databases
PathwayCommonsi | O75478 |
Reactomei | R-HSA-3214847, HATs acetylate histones |
SignaLinki | O75478 |
Names & Taxonomyi
Protein namesi | Recommended name: Transcriptional adapter 2-alphaAlternative name(s): Transcriptional adapter 2-like Short name: ADA2-like protein |
Gene namesi | Name:TADA2A Synonyms:TADA2L ORF Names:KL04P |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:11531, TADA2A |
MIMi | 602276, gene |
neXtProti | NX_O75478 |
Subcellular locationi
Nucleus
- Nucleus 1 Publication
Other locations
- Chromosome By similarity
Cytoskeleton
- mitotic spindle Source: ComplexPortal
Nucleus
- ATAC complex Source: ComplexPortal
- nucleus Source: GO_Central
- SAGA complex Source: UniProtKB
- SAGA-type complex Source: GO_Central
Other locations
- chromosome Source: UniProtKB-SubCell
Keywords - Cellular componenti
Chromosome, NucleusPathology & Biotechi
Organism-specific databases
DisGeNETi | 6871 |
PharmGKBi | PA36306 |
Miscellaneous databases
Pharosi | O75478, Tbio |
Genetic variation databases
BioMutai | TADA2A |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000197083 | 1 – 443 | Transcriptional adapter 2-alphaAdd BLAST | 443 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 6 | Phosphoserine; in variant Ser-6Combined sources | 1 | |
Cross-linki | 132 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources | ||
Cross-linki | 138 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources |
Keywords - PTMi
Isopeptide bond, Phosphoprotein, Ubl conjugationProteomic databases
EPDi | O75478 |
jPOSTi | O75478 |
MassIVEi | O75478 |
MaxQBi | O75478 |
PaxDbi | O75478 |
PeptideAtlasi | O75478 |
PRIDEi | O75478 |
ProteomicsDBi | 50040 [O75478-1] 50041 [O75478-2] |
PTM databases
iPTMneti | O75478 |
PhosphoSitePlusi | O75478 |
Expressioni
Tissue specificityi
Interactioni
Subunit structurei
Interacts with GCN5 and NR3C1. Associated with the P/CAF protein in the PCAF complex.
Component of the PCAF complex, at least composed of TADA2L/ADA2, TADA3L/ADA3, TAF5L/PAF65-beta, TAF6L/PAF65-alpha, TAF10/TAFII30, TAF12/TAFII20, TAF9/TAFII31 and TRRAP.
Component of the ADA2A-containing complex (ATAC), composed of KAT14, KAT2A, TADA2L, TADA3L, ZZ3, MBIP, WDR5, YEATS2, CCDC101 and DR1.
Interacts with CCDC134 (PubMed:22644376).
4 PublicationsBinary interactionsi
O75478
Isoform 2 [O75478-2]
With | #Exp. | IntAct |
---|---|---|
HGS [O14964] | 3 | EBI-16433586,EBI-740220 |
Protein-protein interaction databases
BioGRIDi | 112734, 192 interactors |
ComplexPortali | CPX-1004, PCAF-containing ATAC complex CPX-989, PCAF histone acetylase complex CPX-997, GCN5-containing ATAC complex |
CORUMi | O75478 |
DIPi | DIP-28151N |
IntActi | O75478, 69 interactors |
MINTi | O75478 |
STRINGi | 9606.ENSP00000481091 |
Miscellaneous databases
RNActi | O75478, protein |
Structurei
Secondary structure
3D structure databases
AlphaFoldDBi | O75478 |
SMRi | O75478 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | O75478 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 70 – 122 | SANTPROSITE-ProRule annotationAdd BLAST | 53 | |
Domaini | 356 – 443 | SWIRMPROSITE-ProRule annotationAdd BLAST | 88 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 348 – 372 | DisorderedSequence analysisAdd BLAST | 25 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 349 – 363 | Polar residuesSequence analysisAdd BLAST | 15 |
Zinc finger
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 12 – 69 | ZZ-typePROSITE-ProRule annotationAdd BLAST | 58 |
Keywords - Domaini
Zinc-fingerPhylogenomic databases
eggNOGi | KOG0457, Eukaryota |
InParanoidi | O75478 |
OrthoDBi | 812864at2759 |
PhylomeDBi | O75478 |
TreeFami | TF313975 |
Family and domain databases
CDDi | cd00167, SANT, 1 hit cd02335, ZZ_ADA2, 1 hit |
Gene3Di | 1.10.10.10, 1 hit 3.30.60.90, 1 hit |
InterProi | View protein in InterPro IPR041983, ADA2-like_ZZ IPR016827, Ada2/TADA2 IPR009057, Homeobox-like_sf IPR017930, Myb_dom IPR001005, SANT/Myb IPR017884, SANT_dom IPR007526, SWIRM IPR036388, WH-like_DNA-bd_sf IPR043145, Znf_ZZ_sf |
Pfami | View protein in Pfam PF00249, Myb_DNA-binding, 1 hit PF04433, SWIRM, 1 hit |
PIRSFi | PIRSF025024, Transcriptional_adaptor_2, 1 hit |
SMARTi | View protein in SMART SM00717, SANT, 1 hit |
SUPFAMi | SSF46689, SSF46689, 2 hits |
PROSITEi | View protein in PROSITE PS51293, SANT, 1 hit PS50934, SWIRM, 1 hit PS50135, ZF_ZZ_2, 1 hit |
s (2+)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 9 potential isoforms that are computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MDRLGPFSND PSDKPPCRGC SSYLMEPYIK CAECGPPPFF LCLQCFTRGF
60 70 80 90 100
EYKKHQSDHT YEIMTSDFPV LDPSWTAQEE MALLEAVMDC GFGNWQDVAN
110 120 130 140 150
QMCTKTKEEC EKHYMKHFIN NPLFASTLLN LKQAEEAKTA DTAIPFHSTD
160 170 180 190 200
DPPRPTFDSL LSRDMAGYMP ARADFIEEFD NYAEWDLRDI DFVEDDSDIL
210 220 230 240 250
HALKMAVVDI YHSRLKERQR RKKIIRDHGL INLRKFQLME RRYPKEVQDL
260 270 280 290 300
YETMRRFARI VGPVEHDKFI ESHALEFELR REIKRLQEYR TAGITNFCSA
310 320 330 340 350
RTYDHLKKTR EEERLKRTML SEVLQYIQDS SACQQWLRRQ ADIDSGLSPS
360 370 380 390 400
IPMASNSGRR SAPPLNLTGL PGTEKLNEKE KELCQMVRLV PGAYLEYKSA
410 420 430 440
LLNECNKQGG LRLAQARALI KIDVNKTRKI YDFLIREGYI TKG
Computationally mapped potential isoform sequencesi
There are 9 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketA0A024R0Y4 | A0A024R0Y4_HUMAN | Transcriptional adapter | TADA2A TADA2L, hCG_28187 | 443 | Annotation score: | ||
A0A087WWR4 | A0A087WWR4_HUMAN | Transcriptional adapter 2-alpha | TADA2A TADA2L, hCG_28187 | 305 | Annotation score: | ||
A0A087WU71 | A0A087WU71_HUMAN | Transcriptional adapter 2-alpha | TADA2A | 133 | Annotation score: | ||
A0A0J9YXZ4 | A0A0J9YXZ4_HUMAN | Transcriptional adapter 2-alpha | TADA2A | 133 | Annotation score: | ||
A0A087WZS1 | A0A087WZS1_HUMAN | Transcriptional adapter 2-alpha | TADA2A | 95 | Annotation score: | ||
A0A087X2C8 | A0A087X2C8_HUMAN | Transcriptional adapter 2-alpha | TADA2A | 56 | Annotation score: | ||
A0A087WTV5 | A0A087WTV5_HUMAN | Transcriptional adapter 2-alpha | TADA2A | 194 | Annotation score: | ||
A0A087WYK3 | A0A087WYK3_HUMAN | Transcriptional adapter 2-alpha | TADA2A | 84 | Annotation score: | ||
A0A0J9YWZ4 | A0A0J9YWZ4_HUMAN | Transcriptional adapter 2-alpha | TADA2A | 95 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 117 | H → Y in AAH01172 (PubMed:15489334).Curated | 1 | |
Sequence conflicti | 153 | P → L in AAC39902 (PubMed:9674425).Curated | 1 | |
Sequence conflicti | 185 | W → R in AAC26659 (Ref. 2) Curated | 1 | |
Sequence conflicti | 304 | D → N in AAC26659 (Ref. 2) Curated | 1 | |
Sequence conflicti | 342 | D → G in AAC26659 (Ref. 2) Curated | 1 |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_047466 | 6 | P → SCombined sources4 PublicationsCorresponds to variant dbSNP:rs7211875Ensembl. | 1 | |
Natural variantiVAR_047467 | 115 | M → V1 PublicationCorresponds to variant dbSNP:rs1054865Ensembl. | 1 | |
Natural variantiVAR_047468 | 351 | I → M. Corresponds to variant dbSNP:rs2522969Ensembl. | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_040347 | 275 – 305 | LEFEL…RTYDH → CRWFLSLEQYLCVYIYINRR DNGVFYVKFYK in isoform 2. 1 PublicationAdd BLAST | 31 | |
Alternative sequenceiVSP_040348 | 306 – 443 | Missing in isoform 2. 1 PublicationAdd BLAST | 138 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF064094 mRNA Translation: AAC26659.1 AK022767 mRNA Translation: BAG51111.1 AC004099 Genomic DNA No translation available. AC068400 Genomic DNA No translation available. AC068447 Genomic DNA No translation available. BC001172 mRNA Translation: AAH01172.1 BC011753 mRNA Translation: AAH11753.1 AF069732 mRNA Translation: AAC39902.1 |
CCDSi | CCDS11319.1 [O75478-1] CCDS45656.1 [O75478-2] |
RefSeqi | NP_001159577.2, NM_001166105.2 NP_001278847.1, NM_001291918.1 NP_001479.4, NM_001488.4 NP_597683.3, NM_133439.3 |
Genome annotation databases
Ensembli | ENST00000617828.4; ENSP00000482484.1; ENSG00000277104.4 ENST00000622522.2; ENSP00000481283.1; ENSG00000277104.4 ENST00000633468.1; ENSP00000488016.1; ENSG00000277104.4 ENST00000633584.1; ENSP00000487973.1; ENSG00000277104.4 [O75478-2] |
GeneIDi | 6871 |
KEGGi | hsa:6871 |
UCSCi | uc032ghw.2, human [O75478-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF064094 mRNA Translation: AAC26659.1 AK022767 mRNA Translation: BAG51111.1 AC004099 Genomic DNA No translation available. AC068400 Genomic DNA No translation available. AC068447 Genomic DNA No translation available. BC001172 mRNA Translation: AAH01172.1 BC011753 mRNA Translation: AAH11753.1 AF069732 mRNA Translation: AAC39902.1 |
CCDSi | CCDS11319.1 [O75478-1] CCDS45656.1 [O75478-2] |
RefSeqi | NP_001159577.2, NM_001166105.2 NP_001278847.1, NM_001291918.1 NP_001479.4, NM_001488.4 NP_597683.3, NM_133439.3 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1X41 | NMR | - | A | 72-116 | [»] | |
AlphaFoldDBi | O75478 | |||||
SMRi | O75478 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 112734, 192 interactors |
ComplexPortali | CPX-1004, PCAF-containing ATAC complex CPX-989, PCAF histone acetylase complex CPX-997, GCN5-containing ATAC complex |
CORUMi | O75478 |
DIPi | DIP-28151N |
IntActi | O75478, 69 interactors |
MINTi | O75478 |
STRINGi | 9606.ENSP00000481091 |
PTM databases
iPTMneti | O75478 |
PhosphoSitePlusi | O75478 |
Genetic variation databases
BioMutai | TADA2A |
Proteomic databases
EPDi | O75478 |
jPOSTi | O75478 |
MassIVEi | O75478 |
MaxQBi | O75478 |
PaxDbi | O75478 |
PeptideAtlasi | O75478 |
PRIDEi | O75478 |
ProteomicsDBi | 50040 [O75478-1] 50041 [O75478-2] |
Protocols and materials databases
DNASUi | 6871 |
Genome annotation databases
Ensembli | ENST00000617828.4; ENSP00000482484.1; ENSG00000277104.4 ENST00000622522.2; ENSP00000481283.1; ENSG00000277104.4 ENST00000633468.1; ENSP00000488016.1; ENSG00000277104.4 ENST00000633584.1; ENSP00000487973.1; ENSG00000277104.4 [O75478-2] |
GeneIDi | 6871 |
KEGGi | hsa:6871 |
UCSCi | uc032ghw.2, human [O75478-1] |
Organism-specific databases
CTDi | 6871 |
DisGeNETi | 6871 |
GeneCardsi | TADA2A |
HGNCi | HGNC:11531, TADA2A |
MIMi | 602276, gene |
neXtProti | NX_O75478 |
PharmGKBi | PA36306 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG0457, Eukaryota |
InParanoidi | O75478 |
OrthoDBi | 812864at2759 |
PhylomeDBi | O75478 |
TreeFami | TF313975 |
Enzyme and pathway databases
PathwayCommonsi | O75478 |
Reactomei | R-HSA-3214847, HATs acetylate histones |
SignaLinki | O75478 |
Miscellaneous databases
BioGRID-ORCSi | 6871, 234 hits in 1102 CRISPR screens |
ChiTaRSi | TADA2A, human |
EvolutionaryTracei | O75478 |
GeneWikii | TADA2L |
GenomeRNAii | 6871 |
Pharosi | O75478, Tbio |
PROi | PR:O75478 |
RNActi | O75478, protein |
SOURCEi | Search... |
Family and domain databases
CDDi | cd00167, SANT, 1 hit cd02335, ZZ_ADA2, 1 hit |
Gene3Di | 1.10.10.10, 1 hit 3.30.60.90, 1 hit |
InterProi | View protein in InterPro IPR041983, ADA2-like_ZZ IPR016827, Ada2/TADA2 IPR009057, Homeobox-like_sf IPR017930, Myb_dom IPR001005, SANT/Myb IPR017884, SANT_dom IPR007526, SWIRM IPR036388, WH-like_DNA-bd_sf IPR043145, Znf_ZZ_sf |
Pfami | View protein in Pfam PF00249, Myb_DNA-binding, 1 hit PF04433, SWIRM, 1 hit |
PIRSFi | PIRSF025024, Transcriptional_adaptor_2, 1 hit |
SMARTi | View protein in SMART SM00717, SANT, 1 hit |
SUPFAMi | SSF46689, SSF46689, 2 hits |
PROSITEi | View protein in PROSITE PS51293, SANT, 1 hit PS50934, SWIRM, 1 hit PS50135, ZF_ZZ_2, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | TAD2A_HUMAN | |
Accessioni | O75478Primary (citable) accession number: O75478 Secondary accession number(s): A8MVD0 Q9UP49 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 27, 2001 |
Last sequence update: | January 11, 2011 | |
Last modified: | May 25, 2022 | |
This is version 213 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Human chromosome 17
Human chromosome 17: entries, gene names and cross-references to MIM - Human entries with genetic variants
List of human entries with genetic variants - Human variants curated from literature reports
Index of human variants curated from literature reports - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references