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Protein

PC4 and SFRS1-interacting protein

Gene

PSIP1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Transcriptional coactivator involved in neuroepithelial stem cell differentiation and neurogenesis. Involved in particular in lens epithelial cell gene regulation and stress responses. May play an important role in lens epithelial to fiber cell terminal differentiation. May play a protective role during stress-induced apoptosis. Isoform 2 is a more general and stronger transcriptional coactivator. Isoform 2 may also act as an adapter to coordinate pre-mRNA splicing. Cellular cofactor for lentiviral integration.1 Publication

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDNA-binding
Biological processHost-virus interaction, Transcription, Transcription regulation

Enzyme and pathway databases

ReactomeiR-HSA-162592 Integration of provirus
R-HSA-164843 2-LTR circle formation
R-HSA-175567 Integration of viral DNA into host genomic DNA
R-HSA-177539 Autointegration results in viral DNA circles
R-HSA-180689 APOBEC3G mediated resistance to HIV-1 infection
R-HSA-180910 Vpr-mediated nuclear import of PICs
SIGNORiO75475

Names & Taxonomyi

Protein namesi
Recommended name:
PC4 and SFRS1-interacting protein
Alternative name(s):
CLL-associated antigen KW-7
Dense fine speckles 70 kDa protein
Short name:
DFS 70
Lens epithelium-derived growth factor
Transcriptional coactivator p75/p52
Gene namesi
Name:PSIP1
Synonyms:DFS70, LEDGF, PSIP2
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 9

Organism-specific databases

EuPathDBiHostDB:ENSG00000164985.14
HGNCiHGNC:9527 PSIP1
MIMi603620 gene
neXtProtiNX_O75475

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Involvement in diseasei

A chromosomal aberration involving PSIP1 is associated with pediatric acute myeloid leukemia (AML) with intermediate characteristics between M2-M3 French-American-British (FAB) subtypes. Translocation t(9;11)(p22;p15) with NUP98. The chimeric transcript is an in-frame fusion of NUP98 exon 8 to PSIP1 exon 4.1 Publication

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi360K → A: Reduced interaction with POGZ, CDCA7L and human HIV-1 integrase. 1 Publication1
Mutagenesisi365I → A: Loss of interaction with human HIV-1 integrase; reduced interaction with POGZ and CDCA7L. 2 Publications1
Mutagenesisi366D → A or N: Loss of interaction with human HIV-1 integrase; no effect on interaction with CDCA7L. 2 Publications1
Mutagenesisi366D → A: No effect on interaction with POGZ. 2 Publications1
Mutagenesisi370V → A: Reduced interaction with POGZ, CDCA7L and human HIV-1 integrase. 1 Publication1
Mutagenesisi406F → A: Loss of interaction with human HIV-1 integrase and POGZ; reduced interaction with CDCA7L. 2 Publications1
Mutagenesisi408V → A: Reduced interaction with human HIV-1 integrase; no effect on interaction with POGZ and CDCA7L. 2 Publications1

Organism-specific databases

DisGeNETi11168
OpenTargetsiENSG00000164985
PharmGKBiPA33872

Protein family/group databases

Allergomei2120 Hom s DSF70

Chemistry databases

ChEMBLiCHEMBL3988590

Polymorphism and mutation databases

BioMutaiPSIP1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001917081 – 530PC4 and SFRS1-interacting proteinAdd BLAST530

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Cross-linki75Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Modified residuei102PhosphoserineBy similarity1
Modified residuei105PhosphoserineBy similarity1
Modified residuei106PhosphoserineCombined sources1
Modified residuei115PhosphothreonineBy similarity1
Modified residuei122PhosphothreonineCombined sources1
Modified residuei129PhosphoserineCombined sources1
Modified residuei141PhosphothreonineCombined sources1
Modified residuei167PhosphothreonineCombined sources1
Modified residuei177PhosphoserineCombined sources1
Modified residuei206PhosphoserineCombined sources1
Modified residuei271PhosphoserineCombined sources1
Modified residuei272PhosphothreonineCombined sources1
Modified residuei273PhosphoserineCombined sources1
Modified residuei275PhosphoserineCombined sources1
Modified residuei434PhosphoserineCombined sources1
Modified residuei437PhosphothreonineCombined sources1
Modified residuei443PhosphoserineCombined sources1
Modified residuei514PhosphoserineCombined sources1
Modified residuei517CitrullineBy similarity1
Modified residuei522PhosphoserineCombined sources1
Modified residuei527PhosphothreonineCombined sources1

Post-translational modificationi

Citrullinated by PADI4.By similarity

Keywords - PTMi

Citrullination, Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiO75475
MaxQBiO75475
PaxDbiO75475
PeptideAtlasiO75475
PRIDEiO75475
ProteomicsDBi50037
50038 [O75475-2]
TopDownProteomicsiO75475-1 [O75475-1]
O75475-2 [O75475-2]

PTM databases

iPTMnetiO75475
PhosphoSitePlusiO75475
SwissPalmiO75475

Miscellaneous databases

PMAP-CutDBiO75475

Expressioni

Tissue specificityi

Widely expressed. Expressed at high level in the thymus. Expressed in fetal and adult brain. Expressed in neurons, but not astrocytes. Markedly elevated in fetal as compared to adult brain. In the adult brain, expressed in the subventricular zone (SVZ), in hippocampus, and undetectable elsewhere. In the fetal brain, expressed in the germinal neuroepithelium and cortical plate regions.2 Publications

Gene expression databases

BgeeiENSG00000164985 Expressed in 235 organ(s), highest expression level in secondary oocyte
CleanExiHS_PSIP1
ExpressionAtlasiO75475 baseline and differential
GenevisibleiO75475 HS

Organism-specific databases

HPAiCAB013718
HPA019697

Interactioni

Subunit structurei

Monomer. Interacts with IFRD1/PC4. Isoform 2 interacts with SFRS1. Isoform 1 interacts POGZ and CDCA7L.5 Publications
(Microbial infection) Isoform 1: Interacts with human HIV-1 integrase protein (HIV-1 IN), determining its nuclear localization, its tight association with chromatin and its protection from the proteasome.4 Publications
(Microbial infection) Isoform 1: Interacts with HIV-2 IN.1 Publication

Binary interactionsi

GO - Molecular functioni

Protein-protein interaction databases

BioGridi116339, 55 interactors
CORUMiO75475
DIPiDIP-46656N
IntActiO75475, 27 interactors
MINTiO75475
STRINGi9606.ENSP00000370109

Chemistry databases

BindingDBiO75475

Structurei

Secondary structure

1530
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliO75475
SMRiO75475
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO75475

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini1 – 64PWWPPROSITE-ProRule annotationAdd BLAST64

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili306 – 334Sequence analysisAdd BLAST29
Coiled coili371 – 395Sequence analysisAdd BLAST25

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi146 – 156Nuclear localization signal1 PublicationAdd BLAST11

Domaini

Residues 340-417 are necessary and sufficient for the interaction with HIV-1 IN (IBD domain).

Sequence similaritiesi

Belongs to the HDGF family.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiKOG1904 Eukaryota
ENOG410Y5WD LUCA
GeneTreeiENSGT00530000063013
HOVERGENiHBG108300
InParanoidiO75475
OMAiGNLELWK
OrthoDBiEOG091G045X
PhylomeDBiO75475
TreeFamiTF105385

Family and domain databases

CDDicd05834 HDGF_related, 1 hit
Gene3Di1.20.930.10, 1 hit
InterProiView protein in InterPro
IPR035496 HDGF-rel_PWWP
IPR036218 HIVI-bd_sf
IPR021567 LEDGF_IBD
IPR000313 PWWP_dom
IPR035441 TFIIS/LEDGF_dom_sf
IPR017859 Treacle-like_TCS
PfamiView protein in Pfam
PF11467 LEDGF, 1 hit
PF00855 PWWP, 1 hit
PRINTSiPR01503 TREACLE
SMARTiView protein in SMART
SM00293 PWWP, 1 hit
SUPFAMiSSF140576 SSF140576, 1 hit
PROSITEiView protein in PROSITE
PS50812 PWWP, 1 hit

Sequences (3+)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 3 described isoforms and 1 potential isoform that is computationally mapped.Show allAlign All

Isoform 1 (identifier: O75475-1) [UniParc]FASTAAdd to basket
Also known as: p75, PSIP1

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MTRDFKPGDL IFAKMKGYPH WPARVDEVPD GAVKPPTNKL PIFFFGTHET
60 70 80 90 100
AFLGPKDIFP YSENKEKYGK PNKRKGFNEG LWEIDNNPKV KFSSQQAATK
110 120 130 140 150
QSNASSDVEV EEKETSVSKE DTDHEEKASN EDVTKAVDIT TPKAARRGRK
160 170 180 190 200
RKAEKQVETE EAGVVTTATA SVNLKVSPKR GRPAATEVKI PKPRGRPKMV
210 220 230 240 250
KQPCPSESDI ITEEDKSKKK GQEEKQPKKQ PKKDEEGQKE EDKPRKEPDK
260 270 280 290 300
KEGKKEVESK RKNLAKTGVT STSDSEEEGD DQEGEKKRKG GRNFQTAHRR
310 320 330 340 350
NMLKGQHEKE AADRKRKQEE QMETEQQNKD EGKKPEVKKV EKKRETSMDS
360 370 380 390 400
RLQRIHAEIK NSLKIDNLDV NRCIEALDEL ASLQVTMQQA QKHTEMITTL
410 420 430 440 450
KKIRRFKVSQ VIMEKSTMLY NKFKNMFLVG EGDSVITQVL NKSLAEQRQH
460 470 480 490 500
EEANKTKDQG KKGPNKKLEK EQTGSKTLNG GSDAQDGNQP QHNGESNEDS
510 520 530
KDNHEASTKK KPSSEERETE ISLKDSTLDN
Note: Less active than isoform 2 as transcriptional coactivator, but more abundant in cells.
Length:530
Mass (Da):60,103
Last modified:November 1, 1998 - v1
Checksum:i4B653D02B1D0E174
GO
Isoform 2 (identifier: O75475-2) [UniParc]FASTAAdd to basket
Also known as: p52, PSIP2

The sequence of this isoform differs from the canonical sequence as follows:
     326-333: QQNKDEGK → HQTTCNLQ
     334-530: Missing.

Show »
Length:333
Mass (Da):37,725
Checksum:iDAFEC8F815C06FCD
GO
Isoform 3 (identifier: O75475-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     326-530: QQNKDEGKKP...ISLKDSTLDN → HFAL

Show »
Length:329
Mass (Da):37,268
Checksum:iF35C00DDD27E9BAE
GO

Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
V9GYT7V9GYT7_HUMAN
PC4 and SFRS1-interacting protein
PSIP1
65Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti153 – 161Missing in AAH64135 (PubMed:15489334).Curated9
Sequence conflicti224E → G in AAC97946 (PubMed:9822615).Curated1
Sequence conflicti224E → G in AAC97945 (PubMed:9822615).Curated1
Sequence conflicti272T → P in AAB52589 (PubMed:10856157).Curated1
Sequence conflicti420Y → F in AAC97946 (PubMed:9822615).Curated1
Sequence conflicti420Y → F in AAB52589 (PubMed:10856157).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_044435326 – 530QQNKD…STLDN → HFAL in isoform 3. 1 PublicationAdd BLAST205
Alternative sequenceiVSP_014297326 – 333QQNKDEGK → HQTTCNLQ in isoform 2. 2 Publications8
Alternative sequenceiVSP_014298334 – 530Missing in isoform 2. 2 PublicationsAdd BLAST197

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF098483 mRNA Translation: AAC97946.1
AF098482 mRNA Translation: AAC97945.1
AF063020 mRNA Translation: AAC25167.1
AF199339 Genomic DNA Translation: AAF25870.1
AF199339 Genomic DNA Translation: AAF25871.1
AF432220 mRNA Translation: AAL99926.1
AL359998 Genomic DNA No translation available.
AL441925 Genomic DNA No translation available.
AL513423 Genomic DNA No translation available.
CH471071 Genomic DNA Translation: EAW58677.1
CH471071 Genomic DNA Translation: EAW58678.1
CH471071 Genomic DNA Translation: EAW58679.1
BC044568 mRNA Translation: AAH44568.2
BC064135 mRNA Translation: AAH64135.1
U94319 mRNA Translation: AAB52589.1
CCDSiCCDS6479.1 [O75475-1]
CCDS6480.1 [O75475-2]
CCDS83348.1 [O75475-3]
PIRiJC7168
RefSeqiNP_001121689.1, NM_001128217.2 [O75475-1]
NP_001304827.1, NM_001317898.1 [O75475-3]
NP_001304829.1, NM_001317900.1
NP_066967.3, NM_021144.3 [O75475-2]
NP_150091.2, NM_033222.4 [O75475-1]
UniGeneiHs.658434

Genome annotation databases

EnsembliENST00000380715; ENSP00000370091; ENSG00000164985 [O75475-3]
ENST00000380716; ENSP00000370092; ENSG00000164985 [O75475-2]
ENST00000380733; ENSP00000370109; ENSG00000164985 [O75475-1]
ENST00000380738; ENSP00000370114; ENSG00000164985 [O75475-1]
ENST00000397519; ENSP00000380653; ENSG00000164985 [O75475-2]
GeneIDi11168
KEGGihsa:11168
UCSCiuc003zlv.6 human [O75475-1]

Keywords - Coding sequence diversityi

Alternative splicing, Chromosomal rearrangement

Similar proteinsi

Cross-referencesi

Web resourcesi

Atlas of Genetics and Cytogenetics in Oncology and Haematology

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF098483 mRNA Translation: AAC97946.1
AF098482 mRNA Translation: AAC97945.1
AF063020 mRNA Translation: AAC25167.1
AF199339 Genomic DNA Translation: AAF25870.1
AF199339 Genomic DNA Translation: AAF25871.1
AF432220 mRNA Translation: AAL99926.1
AL359998 Genomic DNA No translation available.
AL441925 Genomic DNA No translation available.
AL513423 Genomic DNA No translation available.
CH471071 Genomic DNA Translation: EAW58677.1
CH471071 Genomic DNA Translation: EAW58678.1
CH471071 Genomic DNA Translation: EAW58679.1
BC044568 mRNA Translation: AAH44568.2
BC064135 mRNA Translation: AAH64135.1
U94319 mRNA Translation: AAB52589.1
CCDSiCCDS6479.1 [O75475-1]
CCDS6480.1 [O75475-2]
CCDS83348.1 [O75475-3]
PIRiJC7168
RefSeqiNP_001121689.1, NM_001128217.2 [O75475-1]
NP_001304827.1, NM_001317898.1 [O75475-3]
NP_001304829.1, NM_001317900.1
NP_066967.3, NM_021144.3 [O75475-2]
NP_150091.2, NM_033222.4 [O75475-1]
UniGeneiHs.658434

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1Z9ENMR-A347-471[»]
2B4JX-ray2.02C/D347-442[»]
2M16NMR-A1-93[»]
2MSRNMR-B344-426[»]
2MTNNMR-A337-442[»]
2N3ANMR-B348-426[»]
3F9KX-ray3.20C/G/K/O/S/W/a/e/i/m/q/u347-435[»]
3HPGX-ray3.28G/H/I/J/K/L347-435[»]
3HPHX-ray2.64E/F/G/H348-435[»]
3U88X-ray3.00C/D347-435[»]
3ZEHNMR-A3-100[»]
4FU6X-ray2.10A1-135[»]
5N88X-ray1.70D347-425[»]
E347-424[»]
5OYMX-ray2.05A/B/C/D/E/F/G/H345-431[»]
5YI9NMR-A345-442[»]
6EMONMR-A345-442[»]
6EMPNMR-A345-442[»]
6EMQNMR-A345-443[»]
6EMRNMR-A345-442[»]
ProteinModelPortaliO75475
SMRiO75475
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi116339, 55 interactors
CORUMiO75475
DIPiDIP-46656N
IntActiO75475, 27 interactors
MINTiO75475
STRINGi9606.ENSP00000370109

Chemistry databases

BindingDBiO75475
ChEMBLiCHEMBL3988590

Protein family/group databases

Allergomei2120 Hom s DSF70

PTM databases

iPTMnetiO75475
PhosphoSitePlusiO75475
SwissPalmiO75475

Polymorphism and mutation databases

BioMutaiPSIP1

Proteomic databases

EPDiO75475
MaxQBiO75475
PaxDbiO75475
PeptideAtlasiO75475
PRIDEiO75475
ProteomicsDBi50037
50038 [O75475-2]
TopDownProteomicsiO75475-1 [O75475-1]
O75475-2 [O75475-2]

Protocols and materials databases

DNASUi11168
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000380715; ENSP00000370091; ENSG00000164985 [O75475-3]
ENST00000380716; ENSP00000370092; ENSG00000164985 [O75475-2]
ENST00000380733; ENSP00000370109; ENSG00000164985 [O75475-1]
ENST00000380738; ENSP00000370114; ENSG00000164985 [O75475-1]
ENST00000397519; ENSP00000380653; ENSG00000164985 [O75475-2]
GeneIDi11168
KEGGihsa:11168
UCSCiuc003zlv.6 human [O75475-1]

Organism-specific databases

CTDi11168
DisGeNETi11168
EuPathDBiHostDB:ENSG00000164985.14
GeneCardsiPSIP1
HGNCiHGNC:9527 PSIP1
HPAiCAB013718
HPA019697
MIMi603620 gene
neXtProtiNX_O75475
OpenTargetsiENSG00000164985
PharmGKBiPA33872
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG1904 Eukaryota
ENOG410Y5WD LUCA
GeneTreeiENSGT00530000063013
HOVERGENiHBG108300
InParanoidiO75475
OMAiGNLELWK
OrthoDBiEOG091G045X
PhylomeDBiO75475
TreeFamiTF105385

Enzyme and pathway databases

ReactomeiR-HSA-162592 Integration of provirus
R-HSA-164843 2-LTR circle formation
R-HSA-175567 Integration of viral DNA into host genomic DNA
R-HSA-177539 Autointegration results in viral DNA circles
R-HSA-180689 APOBEC3G mediated resistance to HIV-1 infection
R-HSA-180910 Vpr-mediated nuclear import of PICs
SIGNORiO75475

Miscellaneous databases

ChiTaRSiPSIP1 human
EvolutionaryTraceiO75475
GeneWikiiPSIP1
GenomeRNAii11168
PMAP-CutDBiO75475
PROiPR:O75475
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000164985 Expressed in 235 organ(s), highest expression level in secondary oocyte
CleanExiHS_PSIP1
ExpressionAtlasiO75475 baseline and differential
GenevisibleiO75475 HS

Family and domain databases

CDDicd05834 HDGF_related, 1 hit
Gene3Di1.20.930.10, 1 hit
InterProiView protein in InterPro
IPR035496 HDGF-rel_PWWP
IPR036218 HIVI-bd_sf
IPR021567 LEDGF_IBD
IPR000313 PWWP_dom
IPR035441 TFIIS/LEDGF_dom_sf
IPR017859 Treacle-like_TCS
PfamiView protein in Pfam
PF11467 LEDGF, 1 hit
PF00855 PWWP, 1 hit
PRINTSiPR01503 TREACLE
SMARTiView protein in SMART
SM00293 PWWP, 1 hit
SUPFAMiSSF140576 SSF140576, 1 hit
PROSITEiView protein in PROSITE
PS50812 PWWP, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiPSIP1_HUMAN
AccessioniPrimary (citable) accession number: O75475
Secondary accession number(s): D3DRI9
, O00256, O95368, Q6P391, Q86YB9, Q9NZI3, Q9UER6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 5, 2005
Last sequence update: November 1, 1998
Last modified: October 10, 2018
This is version 171 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 9
    Human chromosome 9: entries, gene names and cross-references to MIM
  2. SIMILARITY comments
    Index of protein domains and families
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
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