UniProtKB - O75460 (ERN1_HUMAN)
Serine/threonine-protein kinase/endoribonuclease IRE1
ERN1
Functioni
Serine/threonine-protein kinase and endoribonuclease that acts as a key sensor for the endoplasmic reticulum unfolded protein response (UPR) (PubMed:11779464, PubMed:11175748, PubMed:12637535, PubMed:9637683, PubMed:21317875, PubMed:28128204).
In unstressed cells, the endoplasmic reticulum luminal domain is maintained in its inactive monomeric state by binding to the endoplasmic reticulum chaperone HSPA5/BiP (PubMed:21317875).
Accumulation of misfolded proteins in the endoplasmic reticulum causes release of HSPA5/BiP, allowing the luminal domain to homodimerize, promoting autophosphorylation of the kinase domain and subsequent activation of the endoribonuclease activity (PubMed:21317875).
The endoribonuclease activity is specific for XBP1 mRNA and excises 26 nucleotides from XBP1 mRNA (PubMed:11779464, PubMed:24508390, PubMed:21317875).
The resulting spliced transcript of XBP1 encodes a transcriptional activator protein that up-regulates expression of UPR target genes (PubMed:11779464, PubMed:24508390, PubMed:21317875).
Acts as an upstream signal for ER stress-induced GORASP2-mediated unconventional (ER/Golgi-independent) trafficking of CFTR to cell membrane by modulating the expression and localization of SEC16A (PubMed:21884936, PubMed:28067262).
1 Publication8 PublicationsCatalytic activityi
Cofactori
Activity regulationi
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 599 | ATPPROSITE-ProRule annotationCombined sources2 Publications | 1 | |
Active sitei | 688 | Proton acceptorPROSITE-ProRule annotationBy similarity | 1 | |
Binding sitei | 711 | ATPCombined sources1 Publication | 1 | |
Sitei | 892 | Interacts with hydroxy-aryl-aldehyde inhibitorsBy similarity | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 577 – 585 | ATPPROSITE-ProRule annotationBy similarity | 9 | |
Nucleotide bindingi | 643 – 645 | ATPCombined sources1 Publication | 3 | |
Nucleotide bindingi | 690 – 693 | ATPCombined sources1 Publication | 4 |
GO - Molecular functioni
- ADP binding Source: ParkinsonsUK-UCL
- ATP binding Source: UniProtKB
- endoribonuclease activity Source: UniProtKB
- enzyme binding Source: UniProtKB
- Hsp70 protein binding Source: ParkinsonsUK-UCL
- Hsp90 protein binding Source: ParkinsonsUK-UCL
- identical protein binding Source: IntAct
- magnesium ion binding Source: UniProtKB
- platelet-derived growth factor receptor binding Source: BHF-UCL
- protein homodimerization activity Source: UniProtKB
- protein serine/threonine/tyrosine kinase activity Source: RHEA
- protein serine/threonine kinase activity Source: UniProtKB
- protein serine kinase activity Source: RHEA
- unfolded protein binding Source: GO_Central
GO - Biological processi
- cellular response to glucose stimulus Source: ParkinsonsUK-UCL
- cellular response to hydrogen peroxide Source: Ensembl
- cellular response to unfolded protein Source: ParkinsonsUK-UCL
- cellular response to vascular endothelial growth factor stimulus Source: UniProtKB
- endothelial cell proliferation Source: UniProtKB
- insulin metabolic process Source: ParkinsonsUK-UCL
- intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stress Source: UniProtKB
- IRE1-mediated unfolded protein response Source: UniProtKB
- mRNA catabolic process Source: ParkinsonsUK-UCL
- mRNA cleavage Source: UniProtKB
- mRNA cleavage involved in mRNA processing Source: ParkinsonsUK-UCL
- mRNA splicing, via endonucleolytic cleavage and ligation Source: UniProtKB
- peptidyl-serine autophosphorylation Source: ParkinsonsUK-UCL
- peptidyl-serine trans-autophosphorylation Source: ParkinsonsUK-UCL
- positive regulation of endoplasmic reticulum unfolded protein response Source: UniProtKB
- positive regulation of JUN kinase activity Source: ParkinsonsUK-UCL
- positive regulation of RNA splicing Source: UniProtKB
- positive regulation of vascular associated smooth muscle cell proliferation Source: BHF-UCL
- protein autophosphorylation Source: ParkinsonsUK-UCL
- protein phosphorylation Source: UniProtKB
- regulation of macroautophagy Source: ParkinsonsUK-UCL
- response to endoplasmic reticulum stress Source: ParkinsonsUK-UCL
Keywordsi
Molecular function | Hydrolase, Kinase, Multifunctional enzyme, Serine/threonine-protein kinase, Transferase |
Biological process | Apoptosis, Transcription, Transcription regulation, Unfolded protein response |
Ligand | ATP-binding, Magnesium, Metal-binding, Nucleotide-binding |
Enzyme and pathway databases
PathwayCommonsi | O75460 |
Reactomei | R-HSA-381070, IRE1alpha activates chaperones |
SignaLinki | O75460 |
SIGNORi | O75460 |
Protein family/group databases
TCDBi | 8.A.104.1.9, the 5'-amp-activated protein kinase (ampk) family |
Names & Taxonomyi
Protein namesi | Recommended name: Serine/threonine-protein kinase/endoribonuclease IRE1CuratedAlternative name(s): Endoplasmic reticulum-to-nucleus signaling 11 Publication Inositol-requiring protein 11 Publication Short name: hIRE1p1 Publication Ire1-alpha1 Publication Short name: IRE1a1 Publication Including the following 2 domains: Serine/threonine-protein kinase (EC:2.7.11.12 Publications) Endoribonuclease (EC:3.1.26.-1 Publication) |
Gene namesi | |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:3449, ERN1 |
MIMi | 604033, gene |
neXtProti | NX_O75460 |
VEuPathDBi | HostDB:ENSG00000178607 |
Subcellular locationi
Endoplasmic reticulum
- Endoplasmic reticulum membrane 1 Publication; Single-pass type I membrane protein 1 Publication
Endoplasmic reticulum
- endoplasmic reticulum Source: UniProtKB
- endoplasmic reticulum membrane Source: Reactome
- integral component of endoplasmic reticulum membrane Source: UniProtKB
- Ire1 complex Source: ParkinsonsUK-UCL
- IRE1-TRAF2-ASK1 complex Source: ParkinsonsUK-UCL
Mitochondrion
- mitochondrion Source: Ensembl
Nucleus
- nuclear inner membrane Source: Ensembl
Other locations
- AIP1-IRE1 complex Source: Ensembl
- cytoplasm Source: UniProtKB
- IRE1-RACK1-PP2A complex Source: ParkinsonsUK-UCL
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 19 – 443 | LumenalSequence analysisAdd BLAST | 425 | |
Transmembranei | 444 – 464 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 465 – 977 | CytoplasmicSequence analysisAdd BLAST | 513 |
Keywords - Cellular componenti
Endoplasmic reticulum, MembranePathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 105 | Q → E: Impaired ability to homodimerize. 1 Publication | 1 | |
Mutagenesisi | 109 | C → S: No effect on dimerization. 1 Publication | 1 | |
Mutagenesisi | 123 | D → P: Abolishes ability to homodimerize. 1 Publication | 1 | |
Mutagenesisi | 125 | W → A: Abolishes ability to homodimerize. 1 Publication | 1 | |
Mutagenesisi | 148 | C → S: No effect on dimerization. Weakens dimer; when associated with S-332. Abolishes interaction with PDIA6. Prolonged splicing of XBP1, probably due to prolonged activation of PDIA6. Inhibits formation of oxidized multimeric forms of ERN1 in response to ER stress. 2 Publications | 1 | |
Mutagenesisi | 332 | C → S: No effect on dimerization. Weakens dimer; when associated with S-148. 1 Publication | 1 | |
Mutagenesisi | 599 | K → A: Loss of autophosphorylation and of endoribonuclease activity. Inhibition of growth arrest. 2 Publications | 1 |
Organism-specific databases
DisGeNETi | 2081 |
OpenTargetsi | ENSG00000178607 |
PharmGKBi | PA27861 |
Miscellaneous databases
Pharosi | O75460, Tchem |
Chemistry databases
ChEMBLi | CHEMBL1163101 |
DrugBanki | DB12010, Fostamatinib DB07382, N~2~-1H-benzimidazol-5-yl-N~4~-(3-cyclopropyl-1H-pyrazol-5-yl)pyrimidine-2,4-diamine |
DrugCentrali | O75460 |
GuidetoPHARMACOLOGYi | 2020 |
Genetic variation databases
BioMutai | ERN1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 18 | Sequence analysisAdd BLAST | 18 | |
ChainiPRO_0000024327 | 19 – 977 | Serine/threonine-protein kinase/endoribonuclease IRE1Add BLAST | 959 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Glycosylationi | 176 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Modified residuei | 973 | PhosphothreonineCombined sources | 1 |
Post-translational modificationi
Keywords - PTMi
ADP-ribosylation, Disulfide bond, Glycoprotein, PhosphoproteinProteomic databases
EPDi | O75460 |
jPOSTi | O75460 |
MassIVEi | O75460 |
MaxQBi | O75460 |
PaxDbi | O75460 |
PeptideAtlasi | O75460 |
PRIDEi | O75460 |
ProteomicsDBi | 50021 [O75460-1] |
PTM databases
GlyGeni | O75460, 1 site |
iPTMneti | O75460 |
PhosphoSitePlusi | O75460 |
Expressioni
Tissue specificityi
Gene expression databases
Bgeei | ENSG00000178607, Expressed in pancreas and 195 other tissues |
Genevisiblei | O75460, HS |
Organism-specific databases
HPAi | ENSG00000178607, Tissue enhanced (adrenal gland, pancreas) |
Interactioni
Subunit structurei
Monomer (PubMed:29198525, PubMed:16973740). Homodimer; disulfide-linked; homodimerization takes place in response to endoplasmic reticulum stress and promotes activation of the kinase and endoribonuclease activities (PubMed:12637535, PubMed:24508390, PubMed:16973740, PubMed:21317875). Dimer formation is driven by hydrophobic interactions within the N-terminal luminal domains and stabilized by disulfide bridges (PubMed:12637535).
Interacts (via the luminal region) with DNAJB9/ERdj4; interaction takes place in unstressed cells and promotes recruitment of HSPA5/BiP (PubMed:29198525).
Interacts (via the luminal region) with HSPA5/BiP; HSPA5/BiP is a negative regulator of the unfolded protein response (UPR) that prevents homodimerization of ERN1/IRE1 and subsequent activation of the protein (PubMed:12637535, PubMed:29198525).
Interacts with PDIA6, a negative regulator of the UPR; the interaction is direct and disrupts homodimerization (PubMed:24508390).
Interacts with DAB2IP (via PH domain); the interaction occurs in a endoplasmic reticulum stress-induced dependent manner and is required for subsequent recruitment of TRAF2 to ERN1/IRE1 (By similarity).
Interacts with TAOK3 and TRAF2 (PubMed:11278723).
Interacts with RNF13 (PubMed:23378536).
Interacts with LACC1 (PubMed:31875558).
Interacts (when unphosphorylated) with DDRGK1; interaction is dependent on UFM1 and takes place in response to endoplasmic reticulum stress, regulating ERN1/IRE1-alpha stability (PubMed:28128204).
By similarity8 PublicationsBinary interactionsi
O75460
ERN1 - isoform 1 [O75460-1]
With | #Exp. | IntAct |
---|---|---|
itself | 5 | EBI-15600828,EBI-15600828 |
HSPA5 [P11021] | 4 | EBI-15600828,EBI-354921 |
OS9 [Q13438] | 2 | EBI-15600828,EBI-725454 |
SEL1L [Q9UBV2] | 2 | EBI-15600828,EBI-358766 |
SYVN1 [Q86TM6] | 3 | EBI-15600828,EBI-947849 |
Isoform 2 [O75460-2]
GO - Molecular functioni
- enzyme binding Source: UniProtKB
- Hsp70 protein binding Source: ParkinsonsUK-UCL
- Hsp90 protein binding Source: ParkinsonsUK-UCL
- identical protein binding Source: IntAct
- platelet-derived growth factor receptor binding Source: BHF-UCL
- protein homodimerization activity Source: UniProtKB
- unfolded protein binding Source: GO_Central
Protein-protein interaction databases
BioGRIDi | 108391, 58 interactors |
DIPi | DIP-31711N |
IntActi | O75460, 56 interactors |
MINTi | O75460 |
STRINGi | 9606.ENSP00000401445 |
Chemistry databases
BindingDBi | O75460 |
Miscellaneous databases
RNActi | O75460, protein |
Structurei
Secondary structure
3D structure databases
AlphaFoldDBi | O75460 |
SMRi | O75460 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | O75460 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 571 – 832 | Protein kinasePROSITE-ProRule annotationAdd BLAST | 262 | |
Domaini | 835 – 963 | KENPROSITE-ProRule annotationAdd BLAST | 129 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 410 – 434 | DisorderedSequence analysisAdd BLAST | 25 | |
Regioni | 491 – 559 | DisorderedSequence analysisAdd BLAST | 69 | |
Regioni | 906 – 907 | Interacts with hydroxy-aryl-aldehyde inhibitorsBy similarity | 2 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 511 – 551 | Polar residuesSequence analysisAdd BLAST | 41 |
Sequence similaritiesi
Keywords - Domaini
Signal, Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG1027, Eukaryota |
GeneTreei | ENSGT00940000159761 |
HOGENOMi | CLU_004875_1_1_1 |
InParanoidi | O75460 |
OMAi | HYLPDPR |
OrthoDBi | 1019877at2759 |
PhylomeDBi | O75460 |
TreeFami | TF313986 |
Family and domain databases
Gene3Di | 1.20.1440.180, 1 hit 2.130.10.10, 1 hit |
InterProi | View protein in InterPro IPR045133, IRE1/2-like IPR010513, KEN_dom IPR038357, KEN_sf IPR011009, Kinase-like_dom_sf IPR018391, PQQ_beta_propeller_repeat IPR000719, Prot_kinase_dom IPR011047, Quinoprotein_ADH-like_supfam IPR008271, Ser/Thr_kinase_AS IPR015943, WD40/YVTN_repeat-like_dom_sf |
PANTHERi | PTHR13954, PTHR13954, 1 hit |
Pfami | View protein in Pfam PF00069, Pkinase, 1 hit PF06479, Ribonuc_2-5A, 1 hit |
SMARTi | View protein in SMART SM00564, PQQ, 5 hits SM00220, S_TKc, 1 hit |
SUPFAMi | SSF50998, SSF50998, 1 hit SSF56112, SSF56112, 1 hit |
PROSITEi | View protein in PROSITE PS51392, KEN, 1 hit PS50011, PROTEIN_KINASE_DOM, 1 hit PS00108, PROTEIN_KINASE_ST, 1 hit |
s (2+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 2 potential isoforms that are computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MPARRLLLLL TLLLPGLGIF GSTSTVTLPE TLLFVSTLDG SLHAVSKRTG
60 70 80 90 100
SIKWTLKEDP VLQVPTHVEE PAFLPDPNDG SLYTLGSKNN EGLTKLPFTI
110 120 130 140 150
PELVQASPCR SSDGILYMGK KQDIWYVIDL LTGEKQQTLS SAFADSLCPS
160 170 180 190 200
TSLLYLGRTE YTITMYDTKT RELRWNATYF DYAASLPEDD VDYKMSHFVS
210 220 230 240 250
NGDGLVVTVD SESGDVLWIQ NYASPVVAFY VWQREGLRKV MHINVAVETL
260 270 280 290 300
RYLTFMSGEV GRITKWKYPF PKETEAKSKL TPTLYVGKYS TSLYASPSMV
310 320 330 340 350
HEGVAVVPRG STLPLLEGPQ TDGVTIGDKG ECVITPSTDV KFDPGLKSKN
360 370 380 390 400
KLNYLRNYWL LIGHHETPLS ASTKMLERFP NNLPKHRENV IPADSEKKSF
410 420 430 440 450
EEVINLVDQT SENAPTTVSR DVEEKPAHAP ARPEAPVDSM LKDMATIILS
460 470 480 490 500
TFLLIGWVAF IITYPLSMHQ QQQLQHQQFQ KELEKIQLLQ QQQQQLPFHP
510 520 530 540 550
PGDTAQDGEL LDTSGPYSES SGTSSPSTSP RASNHSLCSG SSASKAGSSP
560 570 580 590 600
SLEQDDGDEE TSVVIVGKIS FCPKDVLGHG AEGTIVYRGM FDNRDVAVKR
610 620 630 640 650
ILPECFSFAD REVQLLRESD EHPNVIRYFC TEKDRQFQYI AIELCAATLQ
660 670 680 690 700
EYVEQKDFAH LGLEPITLLQ QTTSGLAHLH SLNIVHRDLK PHNILISMPN
710 720 730 740 750
AHGKIKAMIS DFGLCKKLAV GRHSFSRRSG VPGTEGWIAP EMLSEDCKEN
760 770 780 790 800
PTYTVDIFSA GCVFYYVISE GSHPFGKSLQ RQANILLGAC SLDCLHPEKH
810 820 830 840 850
EDVIARELIE KMIAMDPQKR PSAKHVLKHP FFWSLEKQLQ FFQDVSDRIE
860 870 880 890 900
KESLDGPIVK QLERGGRAVV KMDWRENITV PLQTDLRKFR TYKGGSVRDL
910 920 930 940 950
LRAMRNKKHH YRELPAEVRE TLGSLPDDFV CYFTSRFPHL LAHTYRAMEL
960 970
CSHERLFQPY YFHEPPEPQP PVTPDAL
Computationally mapped potential isoform sequencesi
There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketA0A7P0TAB0 | A0A7P0TAB0_HUMAN | Serine/threonine-protein kinase/end... | ERN1 | 905 | Annotation score: | ||
A0A7P0TA38 | A0A7P0TA38_HUMAN | Serine/threonine-protein kinase/end... | ERN1 | 96 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 190 – 191 | DV → EG in AAC25991 (PubMed:9637683).Curated | 2 | |
Sequence conflicti | 768 | I → V in AAC25991 (PubMed:9637683).Curated | 1 | |
Sequence conflicti | 816 | D → G in BAF85092 (PubMed:14702039).Curated | 1 | |
Sequence conflicti | 824 – 825 | KH → ND in AAC25991 (PubMed:9637683).Curated | 2 | |
Sequence conflicti | 880 | V → D in AAC25991 (PubMed:9637683).Curated | 1 | |
Sequence conflicti | 904 | M → T in BAF85092 (PubMed:14702039).Curated | 1 | |
Sequence conflicti | 924 | S → T in AAC25991 (PubMed:9637683).Curated | 1 |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_040488 | 244 | N → S in a renal clear cell carcinoma sample; somatic mutation. 1 PublicationCorresponds to variant dbSNP:rs1397145500Ensembl. | 1 | |
Natural variantiVAR_040489 | 418 | V → M1 PublicationCorresponds to variant dbSNP:rs55869215Ensembl. | 1 | |
Natural variantiVAR_040490 | 474 | L → R in a lung adenocarcinoma sample; somatic mutation. 1 PublicationCorresponds to variant dbSNP:rs186305118Ensembl. | 1 | |
Natural variantiVAR_040491 | 635 | R → W in a gastric adenocarcinoma sample; somatic mutation. 1 PublicationCorresponds to variant dbSNP:rs146710304Ensembl. | 1 | |
Natural variantiVAR_040492 | 700 | N → S1 PublicationCorresponds to variant dbSNP:rs918253870Ensembl. | 1 | |
Natural variantiVAR_040493 | 769 | S → F in a glioblastoma multiforme sample; somatic mutation. 1 Publication | 1 | |
Natural variantiVAR_040494 | 830 | P → L in an ovarian serous carcinoma sample; somatic mutation. 1 PublicationCorresponds to variant dbSNP:rs1279653488Ensembl. | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_034582 | 19 – 70 | IFGST…THVEE → VSDRGAWGGGQLATAGSGPG QRRGAGAGVRAGSATAAARC PVSPAVGGSGRA in isoform 2. 2 PublicationsAdd BLAST | 52 | |
Alternative sequenceiVSP_034583 | 71 – 977 | Missing in isoform 2. 2 PublicationsAdd BLAST | 907 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF059198 mRNA Translation: AAC25991.1 AK292403 mRNA Translation: BAF85092.1 DA254477 mRNA No translation available. AB209869 mRNA Translation: BAD93106.1 AC005803 Genomic DNA No translation available. AC025362 Genomic DNA No translation available. CH471109 Genomic DNA Translation: EAW94214.1 BC130405 mRNA Translation: AAI30406.1 BC130407 mRNA Translation: AAI30408.1 BI912495 mRNA No translation available. |
CCDSi | CCDS45762.1 [O75460-1] |
RefSeqi | NP_001424.3, NM_001433.3 [O75460-1] |
Genome annotation databases
Ensembli | ENST00000433197.4; ENSP00000401445.2; ENSG00000178607.17 ENST00000606895.2; ENSP00000475519.1; ENSG00000178607.17 [O75460-2] |
GeneIDi | 2081 |
KEGGi | hsa:2081 |
MANE-Selecti | ENST00000433197.4; ENSP00000401445.2; NM_001433.5; NP_001424.3 |
UCSCi | uc002jdz.3, human [O75460-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF059198 mRNA Translation: AAC25991.1 AK292403 mRNA Translation: BAF85092.1 DA254477 mRNA No translation available. AB209869 mRNA Translation: BAD93106.1 AC005803 Genomic DNA No translation available. AC025362 Genomic DNA No translation available. CH471109 Genomic DNA Translation: EAW94214.1 BC130405 mRNA Translation: AAI30406.1 BC130407 mRNA Translation: AAI30408.1 BI912495 mRNA No translation available. |
CCDSi | CCDS45762.1 [O75460-1] |
RefSeqi | NP_001424.3, NM_001433.3 [O75460-1] |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
2HZ6 | X-ray | 3.10 | A | 24-390 | [»] | |
3P23 | X-ray | 2.70 | A/B/C/D | 547-977 | [»] | |
4U6R | X-ray | 2.50 | A | 547-977 | [»] | |
4YZ9 | X-ray | 2.46 | A/B/C | 562-966 | [»] | |
4YZC | X-ray | 2.49 | A/B | 562-966 | [»] | |
4YZD | X-ray | 3.10 | A/B/C | 562-966 | [»] | |
4Z7G | X-ray | 2.60 | A/B | 562-977 | [»] | |
4Z7H | X-ray | 2.90 | A/B | 562-977 | [»] | |
5HGI | X-ray | 2.58 | A | 547-977 | [»] | |
6HV0 | X-ray | 2.73 | A | 562-977 | [»] | |
6HX1 | X-ray | 2.14 | A | 562-964 | [»] | |
6SHC | X-ray | 3.55 | A | 24-390 | [»] | |
6URC | X-ray | 2.20 | A/B | 547-977 | [»] | |
6W39 | X-ray | 1.74 | A/B | 547-977 | [»] | |
6W3A | X-ray | 2.61 | A/B | 547-977 | [»] | |
6W3B | X-ray | 2.57 | A | 547-977 | [»] | |
6W3C | X-ray | 2.30 | A/B/C/D | 547-977 | [»] | |
6W3E | X-ray | 2.74 | A/B | 547-977 | [»] | |
6W3K | X-ray | 2.08 | A | 547-977 | [»] | |
6XDB | X-ray | 2.45 | A | 547-977 | [»] | |
6XDD | X-ray | 2.40 | A/B | 547-977 | [»] | |
6XDF | X-ray | 2.54 | A/B | 547-977 | [»] | |
7BMK | X-ray | 1.85 | A/B | 547-977 | [»] | |
AlphaFoldDBi | O75460 | |||||
SMRi | O75460 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 108391, 58 interactors |
DIPi | DIP-31711N |
IntActi | O75460, 56 interactors |
MINTi | O75460 |
STRINGi | 9606.ENSP00000401445 |
Chemistry databases
BindingDBi | O75460 |
ChEMBLi | CHEMBL1163101 |
DrugBanki | DB12010, Fostamatinib DB07382, N~2~-1H-benzimidazol-5-yl-N~4~-(3-cyclopropyl-1H-pyrazol-5-yl)pyrimidine-2,4-diamine |
DrugCentrali | O75460 |
GuidetoPHARMACOLOGYi | 2020 |
Protein family/group databases
TCDBi | 8.A.104.1.9, the 5'-amp-activated protein kinase (ampk) family |
PTM databases
GlyGeni | O75460, 1 site |
iPTMneti | O75460 |
PhosphoSitePlusi | O75460 |
Genetic variation databases
BioMutai | ERN1 |
Proteomic databases
EPDi | O75460 |
jPOSTi | O75460 |
MassIVEi | O75460 |
MaxQBi | O75460 |
PaxDbi | O75460 |
PeptideAtlasi | O75460 |
PRIDEi | O75460 |
ProteomicsDBi | 50021 [O75460-1] |
Protocols and materials databases
Antibodypediai | 4011, 697 antibodies from 44 providers |
DNASUi | 2081 |
Genome annotation databases
Ensembli | ENST00000433197.4; ENSP00000401445.2; ENSG00000178607.17 ENST00000606895.2; ENSP00000475519.1; ENSG00000178607.17 [O75460-2] |
GeneIDi | 2081 |
KEGGi | hsa:2081 |
MANE-Selecti | ENST00000433197.4; ENSP00000401445.2; NM_001433.5; NP_001424.3 |
UCSCi | uc002jdz.3, human [O75460-1] |
Organism-specific databases
CTDi | 2081 |
DisGeNETi | 2081 |
GeneCardsi | ERN1 |
HGNCi | HGNC:3449, ERN1 |
HPAi | ENSG00000178607, Tissue enhanced (adrenal gland, pancreas) |
MIMi | 604033, gene |
neXtProti | NX_O75460 |
OpenTargetsi | ENSG00000178607 |
PharmGKBi | PA27861 |
VEuPathDBi | HostDB:ENSG00000178607 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG1027, Eukaryota |
GeneTreei | ENSGT00940000159761 |
HOGENOMi | CLU_004875_1_1_1 |
InParanoidi | O75460 |
OMAi | HYLPDPR |
OrthoDBi | 1019877at2759 |
PhylomeDBi | O75460 |
TreeFami | TF313986 |
Enzyme and pathway databases
PathwayCommonsi | O75460 |
Reactomei | R-HSA-381070, IRE1alpha activates chaperones |
SignaLinki | O75460 |
SIGNORi | O75460 |
Miscellaneous databases
BioGRID-ORCSi | 2081, 13 hits in 1109 CRISPR screens |
ChiTaRSi | ERN1, human |
EvolutionaryTracei | O75460 |
GeneWikii | ERN1 |
GenomeRNAii | 2081 |
Pharosi | O75460, Tchem |
PROi | PR:O75460 |
RNActi | O75460, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000178607, Expressed in pancreas and 195 other tissues |
Genevisiblei | O75460, HS |
Family and domain databases
Gene3Di | 1.20.1440.180, 1 hit 2.130.10.10, 1 hit |
InterProi | View protein in InterPro IPR045133, IRE1/2-like IPR010513, KEN_dom IPR038357, KEN_sf IPR011009, Kinase-like_dom_sf IPR018391, PQQ_beta_propeller_repeat IPR000719, Prot_kinase_dom IPR011047, Quinoprotein_ADH-like_supfam IPR008271, Ser/Thr_kinase_AS IPR015943, WD40/YVTN_repeat-like_dom_sf |
PANTHERi | PTHR13954, PTHR13954, 1 hit |
Pfami | View protein in Pfam PF00069, Pkinase, 1 hit PF06479, Ribonuc_2-5A, 1 hit |
SMARTi | View protein in SMART SM00564, PQQ, 5 hits SM00220, S_TKc, 1 hit |
SUPFAMi | SSF50998, SSF50998, 1 hit SSF56112, SSF56112, 1 hit |
PROSITEi | View protein in PROSITE PS51392, KEN, 1 hit PS50011, PROTEIN_KINASE_DOM, 1 hit PS00108, PROTEIN_KINASE_ST, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | ERN1_HUMAN | |
Accessioni | O75460Primary (citable) accession number: O75460 Secondary accession number(s): A1L457 Q59EE2 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | August 16, 2004 |
Last sequence update: | July 1, 2008 | |
Last modified: | May 25, 2022 | |
This is version 208 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Human and mouse protein kinases
Human and mouse protein kinases: classification and index - Human chromosome 17
Human chromosome 17: entries, gene names and cross-references to MIM - Human entries with genetic variants
List of human entries with genetic variants - Human variants curated from literature reports
Index of human variants curated from literature reports - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families