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Protein

Katanin p60 ATPase-containing subunit A1

Gene

KATNA1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalytic subunit of a complex which severs microtubules in an ATP-dependent manner. Microtubule severing may promote rapid reorganization of cellular microtubule arrays and the release of microtubules from the centrosome following nucleation. Microtubule release from the mitotic spindle poles may allow depolymerization of the microtubule end proximal to the spindle pole, leading to poleward microtubule flux and poleward motion of chromosome. Microtubule release within the cell body of neurons may be required for their transport into neuronal processes by microtubule-dependent motor proteins. This transport is required for axonal growth.UniRule annotation3 Publications

Catalytic activityi

ATP + H2O = ADP + phosphate.UniRule annotation

Activity regulationi

ATPase activity is stimulated by microtubules, which promote homooligomerization. ATP-dependent microtubule severing is stimulated by interaction with KATNB1.UniRule annotation

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi249 – 256ATPUniRule annotation8

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • microtubule binding Source: UniProtKB
  • microtubule-severing ATPase activity Source: UniProtKB-EC
  • protein heterodimerization activity Source: UniProtKB

GO - Biological processi

Keywordsi

Molecular functionHydrolase
Biological processCell cycle, Cell division, Mitosis
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi3.6.4.3 2681

Names & Taxonomyi

Protein namesi
Recommended name:
Katanin p60 ATPase-containing subunit A1UniRule annotation (EC:3.6.4.3UniRule annotation)
Short name:
Katanin p60 subunit A1UniRule annotation
Alternative name(s):
p60 kataninUniRule annotation
Gene namesi
Name:KATNA1UniRule annotation
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 6

Organism-specific databases

EuPathDBiHostDB:ENSG00000186625.13
HGNCiHGNC:6216 KATNA1
MIMi606696 gene
neXtProtiNX_O75449

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton, Microtubule

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi255K → A: Abolishes ATP dependent microtubule severing activity and localization to spindle poles. 2 Publications1
Mutagenesisi308D → N: Abolishes ATP dependent microtubule severing activity and localization to spindle poles; when associated with N-309. 1 Publication1
Mutagenesisi309E → N: Abolishes ATP dependent microtubule severing activity and localization to spindle poles; when associated with N-308. 1 Publication1

Organism-specific databases

DisGeNETi11104
OpenTargetsiENSG00000186625
PharmGKBiPA30017

Chemistry databases

ChEMBLiCHEMBL3879856

Polymorphism and mutation databases

BioMutaiKATNA1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000845941 – 491Katanin p60 ATPase-containing subunit A1Add BLAST491

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei42Phosphoserine; by DYRK2UniRule annotation1 Publication1
Modified residuei109Phosphoserine; by DYRK2UniRule annotation1 Publication1
Modified residuei133Phosphothreonine; by DYRK2UniRule annotation1 Publication1
Modified residuei170PhosphoserineCombined sources1

Post-translational modificationi

Phosphorylation by DYRK2 triggers ubiquitination and subsequent degradation.UniRule annotation2 Publications
Ubiquitinated by the BCR(KLHL42) E3 ubiquitin ligase complex, leading to its proteasomal degradation. Ubiquitinated by the EDVP E3 ligase complex and subsequently targeted for proteasomal degradation.UniRule annotation1 Publication

Keywords - PTMi

Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiO75449
MaxQBiO75449
PaxDbiO75449
PeptideAtlasiO75449
PRIDEiO75449
ProteomicsDBi50017
50018 [O75449-2]

PTM databases

iPTMnetiO75449
PhosphoSitePlusiO75449

Expressioni

Gene expression databases

BgeeiENSG00000186625 Expressed in 214 organ(s), highest expression level in testis
CleanExiHS_KATNA1
ExpressionAtlasiO75449 baseline and differential
GenevisibleiO75449 HS

Organism-specific databases

HPAiHPA036207

Interactioni

Subunit structurei

Can homooligomerize into hexameric rings, which may be promoted by interaction with microtubules. Interacts with KATNB1, which may serve as a targeting subunit (PubMed:10751153). Interacts with ASPM; the katanin complex formation KATNA1:KATNB1 is required for the association of ASPM (By similarity). Interacts with dynein and NDEL1. Associates with the E3 ligase complex containing DYRK2, EDD/UBR5, DDB1 and DCAF1 proteins (EDVP complex) (PubMed:19287380). Interacts with KLHL42 (via the kelch domains). Interacts with CUL3; the interaction is enhanced by KLHL42 (PubMed:19261606). Interacts with KATNB1 and KATNBL1 (PubMed:26929214). Interacts with CAMSAP2 and CAMSAP3; leading to regulate the length of CAMSAP-decorated microtubule stretches (PubMed:24486153, PubMed:28386021).By similarity6 Publications

Binary interactionsi

GO - Molecular functioni

Protein-protein interaction databases

BioGridi116285, 51 interactors
IntActiO75449, 44 interactors
STRINGi9606.ENSP00000335106

Structurei

3D structure databases

ProteinModelPortaliO75449
SMRiO75449
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 185Interaction with microtubulesAdd BLAST185
Regioni1 – 75Interaction with dynein and NDEL1By similarityAdd BLAST75
Regioni1 – 29Interaction with KATNB11 PublicationAdd BLAST29

Domaini

The N-terminus is sufficient for interaction with microtubules, although high affinity binding to microtubules also requires an intact C-terminal domain and ATP, which promotes oligomerization.UniRule annotation

Sequence similaritiesi

Belongs to the AAA ATPase family. Katanin p60 subunit A1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiKOG0738 Eukaryota
ENOG410XPN7 LUCA
GeneTreeiENSGT00550000074466
HOGENOMiHOG000225142
HOVERGENiHBG057074
InParanoidiO75449
KOiK07767
OMAiRDEMHMP
OrthoDBiEOG091G0Q8J
PhylomeDBiO75449
TreeFamiTF323170

Family and domain databases

HAMAPiMF_03023 Katanin_p60_A1, 1 hit
InterProiView protein in InterPro
IPR003593 AAA+_ATPase
IPR003959 ATPase_AAA_core
IPR003960 ATPase_AAA_CS
IPR028596 KATNA1
IPR027417 P-loop_NTPase
IPR015415 Vps4_C
PfamiView protein in Pfam
PF00004 AAA, 1 hit
PF09336 Vps4_C, 1 hit
SMARTiView protein in SMART
SM00382 AAA, 1 hit
SUPFAMiSSF52540 SSF52540, 1 hit
PROSITEiView protein in PROSITE
PS00674 AAA, 1 hit

Sequences (2+)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 2 described isoforms and 2 potential isoforms that are computationally mapped.Show allAlign All

Isoform 1 (identifier: O75449-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MSLLMISENV KLAREYALLG NYDSAMVYYQ GVLDQMNKYL YSVKDTYLQQ
60 70 80 90 100
KWQQVWQEIN VEAKHVKDIM KTLESFKLDS TPLKAAQHDL PASEGEVWSM
110 120 130 140 150
PVPVERRPSP GPRKRQSSQY SDPKSHGNRP STTVRVHRSS AQNVHNDRGK
160 170 180 190 200
AVRCREKKEQ NKGREEKNKS PAAVTEPETN KFDSTGYDKD LVEALERDII
210 220 230 240 250
SQNPNVRWDD IADLVEAKKL LKEAVVLPMW MPEFFKGIRR PWKGVLMVGP
260 270 280 290 300
PGTGKTLLAK AVATECKTTF FNVSSSTLTS KYRGESEKLV RLLFEMARFY
310 320 330 340 350
SPATIFIDEI DSICSRRGTS EEHEASRRVK AELLVQMDGV GGTSENDDPS
360 370 380 390 400
KMVMVLAATN FPWDIDEALR RRLEKRIYIP LPSAKGREEL LRISLRELEL
410 420 430 440 450
ADDVDLASIA ENMEGYSGAD ITNVCRDASL MAMRRRIEGL TPEEIRNLSK
460 470 480 490
EEMHMPTTME DFEMALKKVS KSVSAADIER YEKWIFEFGS C
Length:491
Mass (Da):55,965
Last modified:November 1, 1998 - v1
Checksum:iF431594F478491DD
GO
Isoform 2 (identifier: O75449-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     168-243: Missing.
     384-387: AKGR → GMRP
     388-491: Missing.

Note: No experimental confirmation available.
Show »
Length:311
Mass (Da):35,319
Checksum:i9DF635C4E51147C7
GO

Computationally mapped potential isoform sequencesi

There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
B7ZBC8B7ZBC8_HUMAN
Katanin p60 ATPase-containing subun...
KATNA1
280Annotation score:
B7ZBC9B7ZBC9_HUMAN
Katanin p60 ATPase-containing subun...
KATNA1
211Annotation score:

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_012948168 – 243Missing in isoform 2. 1 PublicationAdd BLAST76
Alternative sequenceiVSP_012949384 – 387AKGR → GMRP in isoform 2. 1 Publication4
Alternative sequenceiVSP_012950388 – 491Missing in isoform 2. 1 PublicationAdd BLAST104

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF056022 mRNA Translation: AAC25114.1
AL078581 Genomic DNA No translation available.
BX276089 Genomic DNA No translation available.
CH471051 Genomic DNA Translation: EAW47793.1
CH471051 Genomic DNA Translation: EAW47795.1
BC050428 mRNA Translation: AAH50428.1
CCDSiCCDS5217.1 [O75449-1]
CCDS56456.1 [O75449-2]
RefSeqiNP_001191005.1, NM_001204076.1 [O75449-2]
NP_008975.1, NM_007044.3 [O75449-1]
XP_005266861.1, XM_005266804.2 [O75449-1]
XP_016865696.1, XM_017010207.1 [O75449-1]
XP_016865699.1, XM_017010210.1 [O75449-1]
UniGeneiHs.450175

Genome annotation databases

EnsembliENST00000335643; ENSP00000335180; ENSG00000186625 [O75449-2]
ENST00000335647; ENSP00000335106; ENSG00000186625 [O75449-1]
ENST00000367411; ENSP00000356381; ENSG00000186625 [O75449-1]
GeneIDi11104
KEGGihsa:11104
UCSCiuc003qmr.3 human [O75449-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF056022 mRNA Translation: AAC25114.1
AL078581 Genomic DNA No translation available.
BX276089 Genomic DNA No translation available.
CH471051 Genomic DNA Translation: EAW47793.1
CH471051 Genomic DNA Translation: EAW47795.1
BC050428 mRNA Translation: AAH50428.1
CCDSiCCDS5217.1 [O75449-1]
CCDS56456.1 [O75449-2]
RefSeqiNP_001191005.1, NM_001204076.1 [O75449-2]
NP_008975.1, NM_007044.3 [O75449-1]
XP_005266861.1, XM_005266804.2 [O75449-1]
XP_016865696.1, XM_017010207.1 [O75449-1]
XP_016865699.1, XM_017010210.1 [O75449-1]
UniGeneiHs.450175

3D structure databases

ProteinModelPortaliO75449
SMRiO75449
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi116285, 51 interactors
IntActiO75449, 44 interactors
STRINGi9606.ENSP00000335106

Chemistry databases

ChEMBLiCHEMBL3879856

PTM databases

iPTMnetiO75449
PhosphoSitePlusiO75449

Polymorphism and mutation databases

BioMutaiKATNA1

Proteomic databases

EPDiO75449
MaxQBiO75449
PaxDbiO75449
PeptideAtlasiO75449
PRIDEiO75449
ProteomicsDBi50017
50018 [O75449-2]

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000335643; ENSP00000335180; ENSG00000186625 [O75449-2]
ENST00000335647; ENSP00000335106; ENSG00000186625 [O75449-1]
ENST00000367411; ENSP00000356381; ENSG00000186625 [O75449-1]
GeneIDi11104
KEGGihsa:11104
UCSCiuc003qmr.3 human [O75449-1]

Organism-specific databases

CTDi11104
DisGeNETi11104
EuPathDBiHostDB:ENSG00000186625.13
GeneCardsiKATNA1
HGNCiHGNC:6216 KATNA1
HPAiHPA036207
MIMi606696 gene
neXtProtiNX_O75449
OpenTargetsiENSG00000186625
PharmGKBiPA30017
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG0738 Eukaryota
ENOG410XPN7 LUCA
GeneTreeiENSGT00550000074466
HOGENOMiHOG000225142
HOVERGENiHBG057074
InParanoidiO75449
KOiK07767
OMAiRDEMHMP
OrthoDBiEOG091G0Q8J
PhylomeDBiO75449
TreeFamiTF323170

Enzyme and pathway databases

BRENDAi3.6.4.3 2681

Miscellaneous databases

ChiTaRSiKATNA1 human
GeneWikiiKATNA1
GenomeRNAii11104
PROiPR:O75449
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000186625 Expressed in 214 organ(s), highest expression level in testis
CleanExiHS_KATNA1
ExpressionAtlasiO75449 baseline and differential
GenevisibleiO75449 HS

Family and domain databases

HAMAPiMF_03023 Katanin_p60_A1, 1 hit
InterProiView protein in InterPro
IPR003593 AAA+_ATPase
IPR003959 ATPase_AAA_core
IPR003960 ATPase_AAA_CS
IPR028596 KATNA1
IPR027417 P-loop_NTPase
IPR015415 Vps4_C
PfamiView protein in Pfam
PF00004 AAA, 1 hit
PF09336 Vps4_C, 1 hit
SMARTiView protein in SMART
SM00382 AAA, 1 hit
SUPFAMiSSF52540 SSF52540, 1 hit
PROSITEiView protein in PROSITE
PS00674 AAA, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiKTNA1_HUMAN
AccessioniPrimary (citable) accession number: O75449
Secondary accession number(s): E1P5A3
, Q5TFA8, Q5TFA9, Q86VN2, Q9NU52
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 1, 2005
Last sequence update: November 1, 1998
Last modified: October 10, 2018
This is version 156 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 6
    Human chromosome 6: entries, gene names and cross-references to MIM
  2. SIMILARITY comments
    Index of protein domains and families
  3. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
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