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Protein

Mediator of RNA polymerase II transcription subunit 24

Gene

MED24

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.2 Publications

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionActivator
Biological processTranscription, Transcription regulation

Enzyme and pathway databases

ReactomeiR-HSA-1989781 PPARA activates gene expression
R-HSA-212436 Generic Transcription Pathway
R-HSA-381340 Transcriptional regulation of white adipocyte differentiation

Names & Taxonomyi

Protein namesi
Recommended name:
Mediator of RNA polymerase II transcription subunit 24
Alternative name(s):
Activator-recruited cofactor 100 kDa component
Short name:
ARC100
Cofactor required for Sp1 transcriptional activation subunit 4
Short name:
CRSP complex subunit 4
Mediator complex subunit 24
Thyroid hormone receptor-associated protein 4
Thyroid hormone receptor-associated protein complex 100 kDa component
Short name:
Trap100
Short name:
hTRAP100
Vitamin D3 receptor-interacting protein complex 100 kDa component
Short name:
DRIP100
Gene namesi
Name:MED24
Synonyms:ARC100, CRSP4, DRIP100, KIAA0130, THRAP4, TRAP100
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 17

Organism-specific databases

EuPathDBiHostDB:ENSG00000008838.17
HGNCiHGNC:22963 MED24
MIMi607000 gene
neXtProtiNX_O75448

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Organism-specific databases

DisGeNETi9862
OpenTargetsiENSG00000008838
PharmGKBiPA162395566

Polymorphism and mutation databases

BioMutaiMED24

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000655821 – 989Mediator of RNA polymerase II transcription subunit 24Add BLAST989

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei862PhosphoserineCombined sources1
Modified residuei873PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiO75448
MaxQBiO75448
PaxDbiO75448
PeptideAtlasiO75448
PRIDEiO75448
ProteomicsDBi50015
50016 [O75448-2]

PTM databases

iPTMnetiO75448
PhosphoSitePlusiO75448

Expressioni

Tissue specificityi

Ubiquitous. Abundant in skeletal muscle, heart and placenta.1 Publication

Gene expression databases

BgeeiENSG00000008838 Expressed in 201 organ(s), highest expression level in pituitary gland
CleanExiHS_MED24
ExpressionAtlasiO75448 baseline and differential
GenevisibleiO75448 HS

Interactioni

Subunit structurei

Component of the Mediator complex, which is composed of MED1, MED4, MED6, MED7, MED8, MED9, MED10, MED11, MED12, MED13, MED13L, MED14, MED15, MED16, MED17, MED18, MED19, MED20, MED21, MED22, MED23, MED24, MED25, MED26, MED27, MED29, MED30, MED31, CCNC, CDK8 and CDC2L6/CDK11. The MED12, MED13, CCNC and CDK8 subunits form a distinct module termed the CDK8 module. Mediator containing the CDK8 module is less active than Mediator lacking this module in supporting transcriptional activation. Individual preparations of the Mediator complex lacking one or more distinct subunits have been variously termed ARC, CRSP, DRIP, PC2, SMCC and TRAP. Interacts with AR.8 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
MED16Q9Y2X02EBI-394523,EBI-394541

GO - Molecular functioni

Protein-protein interaction databases

BioGridi115196, 93 interactors
ComplexPortaliCPX-3227 Core mediator complex
CORUMiO75448
DIPiDIP-31462N
IntActiO75448, 37 interactors
MINTiO75448
STRINGi9606.ENSP00000377686

Structurei

3D structure databases

ProteinModelPortaliO75448
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi128 – 132LXXLL motif 15
Motifi344 – 348LXXLL motif 25
Motifi448 – 452LXXLL motif 35
Motifi557 – 561LXXLL motif 45
Motifi788 – 792LXXLL motif 55
Motifi857 – 861LXXLL motif 65

Sequence similaritiesi

Belongs to the Mediator complex subunit 24 family.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiENOG410IF72 Eukaryota
ENOG410YPDT LUCA
GeneTreeiENSGT00390000016438
HOGENOMiHOG000293419
HOVERGENiHBG055588
InParanoidiO75448
KOiK15167
PhylomeDBiO75448
TreeFamiTF323565

Family and domain databases

InterProiView protein in InterPro
IPR021429 Mediator_Med24
PANTHERiPTHR12898 PTHR12898, 1 hit
PfamiView protein in Pfam
PF11277 Med24_N, 1 hit

Sequences (2+)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 2 described isoforms and 19 potential isoforms that are computationally mapped.Show allAlign All

Isoform 1 (identifier: O75448-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MKVVNLKQAI LQAWKERWSD YQWAINMKKF FPKGATWDIL NLADALLEQA
60 70 80 90 100
MIGPSPNPLI LSYLKYAISS QMVSYSSVLT AISKFDDFSR DLCVQALLDI
110 120 130 140 150
MDMFCDRLSC HGKAEECIGL CRALLSALHW LLRCTAASAE RLREGLEAGT
160 170 180 190 200
PAAGEKQLAM CLQRLEKTLS STKNRALLHI AKLEEASSWT AIEHSLLKLG
210 220 230 240 250
EILANLSNPQ LRSQAEQCGT LIRSIPTMLS VHAEQMHKTG FPTVHAVILL
260 270 280 290 300
EGTMNLTGET QSLVEQLTMV KRMQHIPTPL FVLEIWKACF VGLIESPEGT
310 320 330 340 350
EELKWTAFTF LKIPQVLVKL KKYSHGDKDF TEDVNCAFEF LLKLTPLLDK
360 370 380 390 400
ADQRCNCDCT NFLLQECGKQ GLLSEASVNN LMAKRKADRE HAPQQKSGEN
410 420 430 440 450
ANIQPNIQLI LRAEPTVTNI LKTMDADHSK SPEGLLGVLG HMLSGKSLDL
460 470 480 490 500
LLAAAAATGK LKSFARKFIN LNEFTTYGSE ESTKPASVRA LLFDISFLML
510 520 530 540 550
CHVAQTYGSE VILSESRTGA EVPFFETWMQ TCMPEEGKIL NPDHPCFRPD
560 570 580 590 600
STKVESLVAL LNNSSEMKLV QMKWHEACLS ISAAILEILN AWENGVLAFE
610 620 630 640 650
SIQKITDNIK GKVCSLAVCA VAWLVAHVRM LGLDEREKSL QMIRQLAGPL
660 670 680 690 700
FSENTLQFYN ERVVIMNSIL ERMCADVLQQ TATQIKFPST GVDTMPYWNL
710 720 730 740 750
LPPKRPIKEV LTDIFAKVLE KGWVDSRSIH IFDTLLHMGG VYWFCNNLIK
760 770 780 790 800
ELLKETRKEH TLRAVELLYS IFCLDMQQVT LVLLGHILPG LLTDSSKWHS
810 820 830 840 850
LMDPPGTALA KLAVWCALSS YSSHKGQAST RQKKRHREDI EDYISLFPLD
860 870 880 890 900
DVQPSKLMRL LSSNEDDANI LSSPTDRSMS SSLSASQLHT VNMRDPLNRV
910 920 930 940 950
LANLFLLISS ILGSRTAGPH TQFVQWFMEE CVDCLEQGGR GSVLQFMPFT
960 970 980
TVSELVKVSA MSSPKVVLAI TDLSLPLGRQ VAAKAIAAL
Length:989
Mass (Da):110,305
Last modified:November 1, 1998 - v1
Checksum:iCCEDE7D4E74D890C
GO
Isoform 2 (identifier: O75448-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     72-84: Missing.

Show »
Length:976
Mass (Da):108,938
Checksum:iC3628AD29869DF3C
GO

Computationally mapped potential isoform sequencesi

There are 19 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
F5GY88F5GY88_HUMAN
Mediator of RNA polymerase II trans...
MED24
1,008Annotation score:
A0A0B4J1W0A0A0B4J1W0_HUMAN
Mediator of RNA polymerase II trans...
MED24 THRAP4, hCG_30017
1,014Annotation score:
B4DDR8B4DDR8_HUMAN
Mediator of RNA polymerase II trans...
MED24
638Annotation score:
B9TX62B9TX62_HUMAN
Mediator complex subunit MED24 vari...
MED24
292Annotation score:
B9TX75B9TX75_HUMAN
Mediator complex subunit MED24 vari...
MED24
137Annotation score:
F5H5K2F5H5K2_HUMAN
Mediator of RNA polymerase II trans...
MED24
791Annotation score:
E9PFL1E9PFL1_HUMAN
Mediator of RNA polymerase II trans...
MED24
301Annotation score:
J3KSU0J3KSU0_HUMAN
Mediator of RNA polymerase II trans...
MED24
159Annotation score:
H0YEG1H0YEG1_HUMAN
Mediator of RNA polymerase II trans...
MED24
84Annotation score:
F5H0M2F5H0M2_HUMAN
Mediator of RNA polymerase II trans...
MED24
163Annotation score:
There are more potential isoformsShow all

Sequence cautioni

The sequence BAA09479 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti20D → Y in BAA09479 (PubMed:8590280).Curated1
Sequence conflicti476Missing AA sequence (PubMed:10235266).Curated1
Sequence conflicti555E → G in AAF78764 (PubMed:9637681).Curated1
Sequence conflicti710V → E in BAG51081 (PubMed:14702039).Curated1
Sequence conflicti741V → A in BAG51081 (PubMed:14702039).Curated1
Sequence conflicti969A → P in BAG51081 (PubMed:14702039).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_053969204A → T1 PublicationCorresponds to variant dbSNP:rs34585432Ensembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_04112572 – 84Missing in isoform 2. 1 PublicationAdd BLAST13

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF055995 mRNA Translation: AAC39855.1
AF277379 mRNA Translation: AAF78764.1
D50920 mRNA Translation: BAA09479.2 Different initiation.
AK022508 mRNA Translation: BAG51081.1
AK291040 mRNA Translation: BAF83729.1
AC090844 Genomic DNA No translation available.
AC102799 Genomic DNA No translation available.
BC011375 mRNA Translation: AAH11375.1
CCDSiCCDS11359.1 [O75448-1]
CCDS42315.1 [O75448-2]
RefSeqiNP_001072986.1, NM_001079518.1 [O75448-2]
NP_001254726.1, NM_001267797.1 [O75448-2]
NP_055630.2, NM_014815.3 [O75448-1]
XP_016880955.1, XM_017025466.1
UniGeneiHs.462983

Genome annotation databases

EnsembliENST00000356271; ENSP00000348610; ENSG00000008838 [O75448-2]
ENST00000394127; ENSP00000377685; ENSG00000008838 [O75448-2]
ENST00000394128; ENSP00000377686; ENSG00000008838 [O75448-1]
GeneIDi9862
KEGGihsa:9862
UCSCiuc002htt.4 human [O75448-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF055995 mRNA Translation: AAC39855.1
AF277379 mRNA Translation: AAF78764.1
D50920 mRNA Translation: BAA09479.2 Different initiation.
AK022508 mRNA Translation: BAG51081.1
AK291040 mRNA Translation: BAF83729.1
AC090844 Genomic DNA No translation available.
AC102799 Genomic DNA No translation available.
BC011375 mRNA Translation: AAH11375.1
CCDSiCCDS11359.1 [O75448-1]
CCDS42315.1 [O75448-2]
RefSeqiNP_001072986.1, NM_001079518.1 [O75448-2]
NP_001254726.1, NM_001267797.1 [O75448-2]
NP_055630.2, NM_014815.3 [O75448-1]
XP_016880955.1, XM_017025466.1
UniGeneiHs.462983

3D structure databases

ProteinModelPortaliO75448
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi115196, 93 interactors
ComplexPortaliCPX-3227 Core mediator complex
CORUMiO75448
DIPiDIP-31462N
IntActiO75448, 37 interactors
MINTiO75448
STRINGi9606.ENSP00000377686

PTM databases

iPTMnetiO75448
PhosphoSitePlusiO75448

Polymorphism and mutation databases

BioMutaiMED24

Proteomic databases

EPDiO75448
MaxQBiO75448
PaxDbiO75448
PeptideAtlasiO75448
PRIDEiO75448
ProteomicsDBi50015
50016 [O75448-2]

Protocols and materials databases

DNASUi9862
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000356271; ENSP00000348610; ENSG00000008838 [O75448-2]
ENST00000394127; ENSP00000377685; ENSG00000008838 [O75448-2]
ENST00000394128; ENSP00000377686; ENSG00000008838 [O75448-1]
GeneIDi9862
KEGGihsa:9862
UCSCiuc002htt.4 human [O75448-1]

Organism-specific databases

CTDi9862
DisGeNETi9862
EuPathDBiHostDB:ENSG00000008838.17
GeneCardsiMED24
H-InvDBiHIX0174136
HGNCiHGNC:22963 MED24
MIMi607000 gene
neXtProtiNX_O75448
OpenTargetsiENSG00000008838
PharmGKBiPA162395566
HUGEiSearch...
GenAtlasiSearch...

Phylogenomic databases

eggNOGiENOG410IF72 Eukaryota
ENOG410YPDT LUCA
GeneTreeiENSGT00390000016438
HOGENOMiHOG000293419
HOVERGENiHBG055588
InParanoidiO75448
KOiK15167
PhylomeDBiO75448
TreeFamiTF323565

Enzyme and pathway databases

ReactomeiR-HSA-1989781 PPARA activates gene expression
R-HSA-212436 Generic Transcription Pathway
R-HSA-381340 Transcriptional regulation of white adipocyte differentiation

Miscellaneous databases

ChiTaRSiMED24 human
GeneWikiiMED24
GenomeRNAii9862
PROiPR:O75448
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000008838 Expressed in 201 organ(s), highest expression level in pituitary gland
CleanExiHS_MED24
ExpressionAtlasiO75448 baseline and differential
GenevisibleiO75448 HS

Family and domain databases

InterProiView protein in InterPro
IPR021429 Mediator_Med24
PANTHERiPTHR12898 PTHR12898, 1 hit
PfamiView protein in Pfam
PF11277 Med24_N, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiMED24_HUMAN
AccessioniPrimary (citable) accession number: O75448
Secondary accession number(s): A8K4S5
, B3KMR9, Q14143, Q9NNY5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 24, 2002
Last sequence update: November 1, 1998
Last modified: November 7, 2018
This is version 169 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. Human chromosome 17
    Human chromosome 17: entries, gene names and cross-references to MIM
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