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Protein

Histone deacetylase complex subunit SAP30

Gene

SAP30

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Involved in the functional recruitment of the Sin3-histone deacetylase complex (HDAC) to a specific subset of N-CoR corepressor complexes. Capable of transcription repression by N-CoR. Active in deacetylating core histone octamers (when in a complex) but inactive in deacetylating nucleosomal histones.By similarity1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri67 – 115AtypicalAdd BLAST49

GO - Molecular functioni

  • DNA binding Source: UniProtKB-KW
  • histone deacetylase activity Source: GO_Central
  • metal ion binding Source: UniProtKB-KW
  • transcription corepressor activity Source: UniProtKB

GO - Biological processi

Keywordsi

Molecular functionDNA-binding, Repressor
Biological processTranscription, Transcription regulation
LigandMetal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-HSA-3214815 HDACs deacetylate histones
R-HSA-427413 NoRC negatively regulates rRNA expression

Names & Taxonomyi

Protein namesi
Recommended name:
Histone deacetylase complex subunit SAP30
Alternative name(s):
30 kDa Sin3-associated polypeptide
Sin3 corepressor complex subunit SAP30
Sin3-associated polypeptide p30
Gene namesi
Name:SAP30Imported
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 4

Organism-specific databases

EuPathDBiHostDB:ENSG00000164105.3
HGNCiHGNC:10532 SAP30
MIMi603378 gene
neXtProtiNX_O75446

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi67C → A: Abolishes zinc-binding. 1 Publication1
Mutagenesisi68C → A: Retains zinc-binding. 1 Publication1
Mutagenesisi108H → A: Retains zinc-binding. 1 Publication1
Mutagenesisi112C → A: Abolishes zinc-binding. 1 Publication1

Organism-specific databases

OpenTargetsiENSG00000164105
PharmGKBiPA34941

Polymorphism and mutation databases

BioMutaiSAP30

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000975821 – 220Histone deacetylase complex subunit SAP30Add BLAST220

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei5PhosphothreonineCombined sources1
Cross-linki87Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Modified residuei131PhosphoserineCombined sources1
Modified residuei138PhosphoserineCombined sources1
Modified residuei145PhosphothreonineCombined sources1
Cross-linki194Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Cross-linki205Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Cross-linki214Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources

Keywords - PTMi

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiO75446
MaxQBiO75446
PaxDbiO75446
PeptideAtlasiO75446
PRIDEiO75446
ProteomicsDBi50014

PTM databases

CarbonylDBiO75446
GlyConnecti787
iPTMnetiO75446
PhosphoSitePlusiO75446
UniCarbKBiO75446

Expressioni

Tissue specificityi

Expressed in all tissues tested with highest levels in pancreas, ovary, PBL, spleen and thymus; lowest levels in brain, placenta, lung and kidney.1 Publication

Gene expression databases

BgeeiENSG00000164105 Expressed in 212 organ(s), highest expression level in secondary oocyte
CleanExiHS_SAP30
GenevisibleiO75446 HS

Organism-specific databases

HPAiCAB022627
HPA059012

Interactioni

Subunit structurei

Interacts with SIN3A, SIN3B, HDAC2 and NCOR1. Interacts with SAMS1. Component of the histone deacetylase complex that includes at least SIN3A, HDAC1 and HDAC2 (By similarity). Interacts with HDAC1 (PubMed:9651585). Interacts with HCFC1 (PubMed:12670868). Found in a complex composed of at least SINHCAF, SIN3A, HDAC1, SAP30, RBBP4, OGT and TET1 (By similarity).By similarity2 Publications

Protein-protein interaction databases

BioGridi114346, 57 interactors
ComplexPortaliCPX-3321 SIN3A histone deacetylase complex
CPX-3322 SIN3B histone deacetylase complex
CPX-3323 SIN3A histone deacetylase complex, ES cell-specific variant
CORUMiO75446
DIPiDIP-33328N
IntActiO75446, 36 interactors
MINTiO75446
STRINGi9606.ENSP00000296504

Structurei

Secondary structure

1220
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliO75446
SMRiO75446
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO75446

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 129Interaction with NCOR1By similarityAdd BLAST129
Regioni130 – 220Interaction with SIN3ABy similarityAdd BLAST91

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi15 – 59Ala-richSequence analysisAdd BLAST45

Sequence similaritiesi

Belongs to the SAP30 family.Curated

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri67 – 115AtypicalAdd BLAST49

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiENOG410IHMG Eukaryota
ENOG4111GVC LUCA
GeneTreeiENSGT00390000006633
HOGENOMiHOG000007811
HOVERGENiHBG057907
InParanoidiO75446
KOiK19202
OMAiFTPEDMS
OrthoDBiEOG091G0PSQ
PhylomeDBiO75446
TreeFamiTF324135

Family and domain databases

Gene3Di1.10.720.110, 1 hit
InterProiView protein in InterPro
IPR024145 His_deAcase_SAP30/SAP30L
IPR038291 SAP30_C_sf
IPR025718 SAP30_Sin3-bd
IPR025717 SAP30_zn-finger
PANTHERiPTHR13286 PTHR13286, 1 hit
PfamiView protein in Pfam
PF13867 SAP30_Sin3_bdg, 1 hit
PF13866 zf-SAP30, 1 hit

Sequencei

Sequence statusi: Complete.

O75446-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MNGFTPDEMS RGGDAAAAVA AVVAAAAAAA SAGNGTGAGT GAEVPGAGAV
60 70 80 90 100
SAAGPPGAAG PGPGQLCCLR EDGERCGRAA GNASFSKRIQ KSISQKKVKI
110 120 130 140 150
ELDKSARHLY ICDYHKNLIQ SVRNRRKRKG SDDDGGDSPV QDIDTPEVDL
160 170 180 190 200
YQLQVNTLRR YKRHFKLPTR PGLNKAQLVE IVGCHFRSIP VNEKDTLTYF
210 220
IYSVKNDKNK SDLKVDSGVH
Length:220
Mass (Da):23,306
Last modified:November 1, 1998 - v1
Checksum:iFF84E06B17AFCAC2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF055993 mRNA Translation: AAC33316.1
CH471056 Genomic DNA Translation: EAX04755.1
CH471056 Genomic DNA Translation: EAX04756.1
BC016757 mRNA Translation: AAH16757.1
CCDSiCCDS3817.1
RefSeqiNP_003855.1, NM_003864.3
UniGeneiHs.591715

Genome annotation databases

EnsembliENST00000296504; ENSP00000296504; ENSG00000164105
GeneIDi8819
KEGGihsa:8819
UCSCiuc003itd.4 human

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF055993 mRNA Translation: AAC33316.1
CH471056 Genomic DNA Translation: EAX04755.1
CH471056 Genomic DNA Translation: EAX04756.1
BC016757 mRNA Translation: AAH16757.1
CCDSiCCDS3817.1
RefSeqiNP_003855.1, NM_003864.3
UniGeneiHs.591715

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2KDPNMR-A64-131[»]
ProteinModelPortaliO75446
SMRiO75446
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi114346, 57 interactors
ComplexPortaliCPX-3321 SIN3A histone deacetylase complex
CPX-3322 SIN3B histone deacetylase complex
CPX-3323 SIN3A histone deacetylase complex, ES cell-specific variant
CORUMiO75446
DIPiDIP-33328N
IntActiO75446, 36 interactors
MINTiO75446
STRINGi9606.ENSP00000296504

PTM databases

CarbonylDBiO75446
GlyConnecti787
iPTMnetiO75446
PhosphoSitePlusiO75446
UniCarbKBiO75446

Polymorphism and mutation databases

BioMutaiSAP30

Proteomic databases

EPDiO75446
MaxQBiO75446
PaxDbiO75446
PeptideAtlasiO75446
PRIDEiO75446
ProteomicsDBi50014

Protocols and materials databases

DNASUi8819
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000296504; ENSP00000296504; ENSG00000164105
GeneIDi8819
KEGGihsa:8819
UCSCiuc003itd.4 human

Organism-specific databases

CTDi8819
EuPathDBiHostDB:ENSG00000164105.3
GeneCardsiSAP30
HGNCiHGNC:10532 SAP30
HPAiCAB022627
HPA059012
MIMi603378 gene
neXtProtiNX_O75446
OpenTargetsiENSG00000164105
PharmGKBiPA34941
GenAtlasiSearch...

Phylogenomic databases

eggNOGiENOG410IHMG Eukaryota
ENOG4111GVC LUCA
GeneTreeiENSGT00390000006633
HOGENOMiHOG000007811
HOVERGENiHBG057907
InParanoidiO75446
KOiK19202
OMAiFTPEDMS
OrthoDBiEOG091G0PSQ
PhylomeDBiO75446
TreeFamiTF324135

Enzyme and pathway databases

ReactomeiR-HSA-3214815 HDACs deacetylate histones
R-HSA-427413 NoRC negatively regulates rRNA expression

Miscellaneous databases

ChiTaRSiSAP30 human
EvolutionaryTraceiO75446
GeneWikiiSAP30
GenomeRNAii8819
PROiPR:O75446
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000164105 Expressed in 212 organ(s), highest expression level in secondary oocyte
CleanExiHS_SAP30
GenevisibleiO75446 HS

Family and domain databases

Gene3Di1.10.720.110, 1 hit
InterProiView protein in InterPro
IPR024145 His_deAcase_SAP30/SAP30L
IPR038291 SAP30_C_sf
IPR025718 SAP30_Sin3-bd
IPR025717 SAP30_zn-finger
PANTHERiPTHR13286 PTHR13286, 1 hit
PfamiView protein in Pfam
PF13867 SAP30_Sin3_bdg, 1 hit
PF13866 zf-SAP30, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiSAP30_HUMAN
AccessioniPrimary (citable) accession number: O75446
Secondary accession number(s): D3DP38
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 21, 2005
Last sequence update: November 1, 1998
Last modified: November 7, 2018
This is version 145 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Human chromosome 4
    Human chromosome 4: entries, gene names and cross-references to MIM
  3. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  4. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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