UniProtKB - O75436 (VP26A_HUMAN)
Vacuolar protein sorting-associated protein 26A
VPS26A
Functioni
GO - Biological processi
- intracellular protein transport Source: GO_Central
- regulation of macroautophagy Source: ParkinsonsUK-UCL
- retrograde transport, endosome to Golgi Source: UniProtKB
- retrograde transport, endosome to plasma membrane Source: UniProtKB
- Wnt signaling pathway Source: Reactome
Keywordsi
Biological process | Protein transport, Transport |
Enzyme and pathway databases
PathwayCommonsi | O75436 |
Reactomei | R-HSA-3238698, WNT ligand biogenesis and trafficking |
Protein family/group databases
TCDBi | 9.A.3.1.1, the sorting nexin27 (snx27)-retromer assembly apparatus |
Names & Taxonomyi
Protein namesi | Recommended name: Vacuolar protein sorting-associated protein 26AAlternative name(s): Vesicle protein sorting 26A Short name: hVPS26 |
Gene namesi | Name:VPS26A Synonyms:VPS26 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
EuPathDBi | HostDB:ENSG00000122958.14 |
HGNCi | HGNC:12711, VPS26A |
MIMi | 605506, gene |
neXtProti | NX_O75436 |
Subcellular locationi
Endosome
- Endosome membrane 1 Publication; Peripheral membrane protein By similarity
- Early endosome 4 Publications
Other locations
- Cytoplasm 1 Publication
Note: Localizes to tubular profiles adjacent to endosomes (PubMed:15078903). Predominantly found in early not late endosomes (By similarity).By similarity
Cytosol
- cytosol Source: Reactome
Endosome
- early endosome Source: UniProtKB
- endosome Source: UniProtKB
- endosome membrane Source: UniProtKB
- tubular endosome Source: UniProtKB
Lysosome
- lysosome Source: HPA
Other locations
- retromer complex Source: UniProtKB
- retromer, cargo-selective complex Source: ParkinsonsUK-UCL
- vesicle Source: UniProtKB
Keywords - Cellular componenti
Cytoplasm, Endosome, MembranePathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 235 – 236 | IM → DD: Abolishes interaction with VPS35 and endosomal subcellular location. | 2 |
Organism-specific databases
DisGeNETi | 9559 |
OpenTargetsi | ENSG00000122958 |
PharmGKBi | PA37326 |
Miscellaneous databases
Pharosi | O75436, Tbio |
Polymorphism and mutation databases
BioMutai | VPS26A |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000073007 | 1 – 327 | Vacuolar protein sorting-associated protein 26AAdd BLAST | 327 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 315 | PhosphoserineCombined sources | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
EPDi | O75436 |
jPOSTi | O75436 |
MassIVEi | O75436 |
MaxQBi | O75436 |
PaxDbi | O75436 |
PeptideAtlasi | O75436 |
PRIDEi | O75436 |
ProteomicsDBi | 2454 50002 [O75436-1] |
PTM databases
iPTMneti | O75436 |
MetOSitei | O75436 |
PhosphoSitePlusi | O75436 |
SwissPalmi | O75436 |
Expressioni
Inductioni
Gene expression databases
Bgeei | ENSG00000122958, Expressed in visceral pleura and 245 other tissues |
ExpressionAtlasi | O75436, baseline and differential |
Genevisiblei | O75436, HS |
Organism-specific databases
HPAi | ENSG00000122958, Low tissue specificity |
Interactioni
Subunit structurei
Component of the heterotrimeric retromer cargo-selective complex (CSC), also described as vacuolar protein sorting subcomplex (VPS), formed by VPS26 (VPS26A or VPS26B), VPS29 and VPS35 (PubMed:11102511, PubMed:28892079). The CSC has a highly elongated structure with VPS26 and VPS29 binding independently at opposite distal ends of VPS35 as central platform (By similarity). The CSC is believed to associate with variable sorting nexins to form functionally distinct retromer complex variants. The originally described retromer complex (also called SNX-BAR retromer) is a pentamer containing the CSC and a heterodimeric membrane-deforming subcomplex formed between SNX1 or SNX2 and SNX5 or SNX6 (also called SNX-BAR subcomplex); the respective CSC and SNX-BAR subcomplexes associate with low affinity. The CSC associates with SNX3 to form a SNX3-retromer complex. The CSC associates with SNX27, the WASH complex and the SNX-BAR subcomplex to form the SNX27-retromer complex (Probable).
Interacts with VPS29, VPS35, SNX1, SNX2, SNX5, SNX6, SNX3, SNX27, RAB7A, ECPAS, EHD1, WASHC5, SORL1 (PubMed:11102511, PubMed:16190980, PubMed:19619496, PubMed:17868075, PubMed:19531583, PubMed:20682791, PubMed:20923837, PubMed:21725319, PubMed:22279231, PubMed:24344282, PubMed:16732284, PubMed:28892079).
3 PublicationsBy similarity12 PublicationsBinary interactionsi
Hide detailsO75436
With | #Exp. | IntAct |
---|---|---|
MORF4L1 - isoform 2 [Q9UBU8-2] | 3 | EBI-1043891,EBI-10288852 |
VPS29 [Q9UBQ0] | 9 | EBI-1043891,EBI-718596 |
VPS35 [Q96QK1] | 19 | EBI-1043891,EBI-1054634 |
ZSCAN12 [O43309] | 3 | EBI-1043891,EBI-1210440 |
Protein-protein interaction databases
BioGRIDi | 114930, 124 interactors |
CORUMi | O75436 |
DIPi | DIP-29075N |
IntActi | O75436, 58 interactors |
MINTi | O75436 |
STRINGi | 9606.ENSP00000362480 |
Miscellaneous databases
RNActi | O75436, protein |
Structurei
Secondary structure
3D structure databases
SMRi | O75436 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | O75436 |
Family & Domainsi
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG3063, Eukaryota |
GeneTreei | ENSGT00950000183064 |
HOGENOMi | CLU_031077_0_0_1 |
InParanoidi | O75436 |
OMAi | KPRTNFH |
OrthoDBi | 987411at2759 |
PhylomeDBi | O75436 |
TreeFami | TF300907 |
Family and domain databases
Gene3Di | 2.60.40.640, 2 hits |
InterProi | View protein in InterPro IPR014752, Arrestin_C IPR028934, Vps26-related |
PANTHERi | PTHR12233, PTHR12233, 1 hit |
Pfami | View protein in Pfam PF03643, Vps26, 1 hit |
s (2+)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 2 potential isoforms that are computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MSFLGGFFGP ICEIDIVLND GETRKMAEMK TEDGKVEKHY LFYDGESVSG
60 70 80 90 100
KVNLAFKQPG KRLEHQGIRI EFVGQIELFN DKSNTHEFVN LVKELALPGE
110 120 130 140 150
LTQSRSYDFE FMQVEKPYES YIGANVRLRY FLKVTIVRRL TDLVKEYDLI
160 170 180 190 200
VHQLATYPDV NNSIKMEVGI EDCLHIEFEY NKSKYHLKDV IVGKIYFLLV
210 220 230 240 250
RIKIQHMELQ LIKKEITGIG PSTTTETETI AKYEIMDGAP VKGESIPIRL
260 270 280 290 300
FLAGYDPTPT MRDVNKKFSV RYFLNLVLVD EEDRRYFKQQ EIILWRKAPE
310 320
KLRKQRTNFH QRFESPESQA SAEQPEM
Computationally mapped potential isoform sequencesi
There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketS4R3Q6 | S4R3Q6_HUMAN | Vacuolar protein sorting-associated... | VPS26A | 226 | Annotation score: | ||
S4R2Y3 | S4R2Y3_HUMAN | Vacuolar protein sorting-associated... | VPS26A | 63 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length | |
---|---|---|---|---|---|
Sequence conflicti | 75 | Q → R in BAB14351 (PubMed:14702039).Curated | 1 | ||
Sequence conflicti | 242 | K → Q in BQ048905 (PubMed:15489334).Curated | 1 | ||
Sequence conflicti | 285 – 286 | RY → SS in AAF89954 (PubMed:11102511).Curated | 2 | ||
Sequence conflicti | 285 – 286 | RY → SS in AAC39912 (PubMed:9653160).Curated | 2 | ||
Isoform 2 (identifier: O75436-2) | |||||
Sequence conflicti | 243 | G → R in BQ048905 (PubMed:15489334).Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_044910 | 243 – 327 | GESIP…EQPEM → GDNFMEKSS in isoform 2. 1 PublicationAdd BLAST | 85 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF175266 mRNA Translation: AAF89954.1 AF054179 mRNA Translation: AAC39912.1 AK022992 mRNA Translation: BAB14351.1 AK315496 mRNA Translation: BAG37880.1 AL596223 Genomic DNA No translation available. AL442635 Genomic DNA No translation available. CH471083 Genomic DNA Translation: EAW54313.1 BC022505 mRNA Translation: AAH22505.1 BQ048905 mRNA No translation available. |
CCDSi | CCDS41536.1 [O75436-2] CCDS7286.1 [O75436-1] |
RefSeqi | NP_001030337.1, NM_001035260.2 [O75436-2] NP_001305875.1, NM_001318946.1 NP_004887.2, NM_004896.4 [O75436-1] |
Genome annotation databases
Ensembli | ENST00000263559; ENSP00000263559; ENSG00000122958 [O75436-1] ENST00000373382; ENSP00000362480; ENSG00000122958 [O75436-1] ENST00000395098; ENSP00000378532; ENSG00000122958 [O75436-2] |
GeneIDi | 9559 |
KEGGi | hsa:9559 |
UCSCi | uc001jpb.4, human [O75436-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF175266 mRNA Translation: AAF89954.1 AF054179 mRNA Translation: AAC39912.1 AK022992 mRNA Translation: BAB14351.1 AK315496 mRNA Translation: BAG37880.1 AL596223 Genomic DNA No translation available. AL442635 Genomic DNA No translation available. CH471083 Genomic DNA Translation: EAW54313.1 BC022505 mRNA Translation: AAH22505.1 BQ048905 mRNA No translation available. |
CCDSi | CCDS41536.1 [O75436-2] CCDS7286.1 [O75436-1] |
RefSeqi | NP_001030337.1, NM_001035260.2 [O75436-2] NP_001305875.1, NM_001318946.1 NP_004887.2, NM_004896.4 [O75436-1] |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
2FAU | X-ray | 2.10 | A | 1-327 | [»] | |
5F0J | X-ray | 2.70 | B | 2-326 | [»] | |
5F0L | X-ray | 3.20 | B | 1-317 | [»] | |
5F0M | X-ray | 3.10 | B | 1-321 | [»] | |
5F0P | X-ray | 2.78 | B | 1-321 | [»] | |
SMRi | O75436 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 114930, 124 interactors |
CORUMi | O75436 |
DIPi | DIP-29075N |
IntActi | O75436, 58 interactors |
MINTi | O75436 |
STRINGi | 9606.ENSP00000362480 |
Protein family/group databases
TCDBi | 9.A.3.1.1, the sorting nexin27 (snx27)-retromer assembly apparatus |
PTM databases
iPTMneti | O75436 |
MetOSitei | O75436 |
PhosphoSitePlusi | O75436 |
SwissPalmi | O75436 |
Polymorphism and mutation databases
BioMutai | VPS26A |
Proteomic databases
EPDi | O75436 |
jPOSTi | O75436 |
MassIVEi | O75436 |
MaxQBi | O75436 |
PaxDbi | O75436 |
PeptideAtlasi | O75436 |
PRIDEi | O75436 |
ProteomicsDBi | 2454 50002 [O75436-1] |
Protocols and materials databases
Antibodypediai | 28695, 193 antibodies |
DNASUi | 9559 |
Genome annotation databases
Ensembli | ENST00000263559; ENSP00000263559; ENSG00000122958 [O75436-1] ENST00000373382; ENSP00000362480; ENSG00000122958 [O75436-1] ENST00000395098; ENSP00000378532; ENSG00000122958 [O75436-2] |
GeneIDi | 9559 |
KEGGi | hsa:9559 |
UCSCi | uc001jpb.4, human [O75436-1] |
Organism-specific databases
CTDi | 9559 |
DisGeNETi | 9559 |
EuPathDBi | HostDB:ENSG00000122958.14 |
GeneCardsi | VPS26A |
HGNCi | HGNC:12711, VPS26A |
HPAi | ENSG00000122958, Low tissue specificity |
MIMi | 605506, gene |
neXtProti | NX_O75436 |
OpenTargetsi | ENSG00000122958 |
PharmGKBi | PA37326 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG3063, Eukaryota |
GeneTreei | ENSGT00950000183064 |
HOGENOMi | CLU_031077_0_0_1 |
InParanoidi | O75436 |
OMAi | KPRTNFH |
OrthoDBi | 987411at2759 |
PhylomeDBi | O75436 |
TreeFami | TF300907 |
Enzyme and pathway databases
PathwayCommonsi | O75436 |
Reactomei | R-HSA-3238698, WNT ligand biogenesis and trafficking |
Miscellaneous databases
BioGRID-ORCSi | 9559, 60 hits in 823 CRISPR screens |
ChiTaRSi | VPS26A, human |
EvolutionaryTracei | O75436 |
GeneWikii | VPS26A |
GenomeRNAii | 9559 |
Pharosi | O75436, Tbio |
PROi | PR:O75436 |
RNActi | O75436, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000122958, Expressed in visceral pleura and 245 other tissues |
ExpressionAtlasi | O75436, baseline and differential |
Genevisiblei | O75436, HS |
Family and domain databases
Gene3Di | 2.60.40.640, 2 hits |
InterProi | View protein in InterPro IPR014752, Arrestin_C IPR028934, Vps26-related |
PANTHERi | PTHR12233, PTHR12233, 1 hit |
Pfami | View protein in Pfam PF03643, Vps26, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | VP26A_HUMAN | |
Accessioni | O75436Primary (citable) accession number: O75436 Secondary accession number(s): A8MZ56 Q9H982 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 21, 2001 |
Last sequence update: | November 25, 2002 | |
Last modified: | December 2, 2020 | |
This is version 182 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families - Human chromosome 10
Human chromosome 10: entries, gene names and cross-references to MIM