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Protein

Tripartite motif-containing protein 3

Gene

TRIM3

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Probably involved in vesicular trafficking via its association with the CART complex (PubMed:15772161). The CART complex is necessary for efficient transferrin receptor recycling but not for EGFR degradation (PubMed:15772161). Positively regulates motility of microtubule-dependent motor protein KIF21B (By similarity).By similarity1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri22 – 63RING-typePROSITE-ProRule annotationAdd BLAST42
Zinc fingeri110 – 151B box-typePROSITE-ProRule annotationAdd BLAST42

GO - Molecular functioni

  • protein C-terminus binding Source: ProtInc
  • ubiquitin protein ligase activity Source: Ensembl
  • zinc ion binding Source: InterPro

GO - Biological processi

Keywordsi

Biological processProtein transport, Transport
LigandMetal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-HSA-877300 Interferon gamma signaling

Names & Taxonomyi

Protein namesi
Recommended name:
Tripartite motif-containing protein 3
Alternative name(s):
Brain-expressed RING finger protein
RING finger protein 22
RING finger protein 97
Gene namesi
Name:TRIM3
Synonyms:BERP, RNF22, RNF97
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 11

Organism-specific databases

EuPathDBiHostDB:ENSG00000110171.18
HGNCiHGNC:10064 TRIM3
MIMi605493 gene
neXtProtiNX_O75382

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell projection, Cytoplasm, Endosome, Golgi apparatus

Pathology & Biotechi

Organism-specific databases

DisGeNETi10612
OpenTargetsiENSG00000110171
PharmGKBiPA35534

Polymorphism and mutation databases

BioMutaiTRIM3

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources
ChainiPRO_00000561972 – 744Tripartite motif-containing protein 3Add BLAST743

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineCombined sources1
Modified residuei7PhosphoserineCombined sources1
Modified residuei427PhosphoserineCombined sources1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiO75382
MaxQBiO75382
PaxDbiO75382
PeptideAtlasiO75382
PRIDEiO75382
ProteomicsDBi49955
49956 [O75382-2]
49957 [O75382-3]

PTM databases

iPTMnetiO75382
PhosphoSitePlusiO75382

Expressioni

Tissue specificityi

Expressed in brain, heart, uterus and testis.

Gene expression databases

BgeeiENSG00000110171 Expressed in 192 organ(s), highest expression level in right hemisphere of cerebellum
CleanExiHS_TRIM3
ExpressionAtlasiO75382 baseline and differential
GenevisibleiO75382 HS

Organism-specific databases

HPAiHPA043396
HPA048233

Interactioni

Subunit structurei

Associates with myosin-Vb (MYO5B) and alpha-actinin-4 (ACTN4) (By similarity). Component of the CART complex, at least composed of ACTN4, HGS/HRS, MYO5B and TRIM3 (PubMed:15772161). Interacts with ZFYVE28/LST2 (PubMed:19460345). Interacts with KIF21B (By similarity).By similarity2 Publications

Binary interactionsi

GO - Molecular functioni

Protein-protein interaction databases

BioGridi115858, 23 interactors
CORUMiO75382
IntActiO75382, 22 interactors
MINTiO75382
STRINGi9606.ENSP00000340797

Structurei

3D structure databases

ProteinModelPortaliO75382
SMRiO75382
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati317 – 418FilaminAdd BLAST102
Repeati473 – 516NHL 1Add BLAST44
Repeati520 – 563NHL 2Add BLAST44
Repeati564 – 605NHL 3Add BLAST42
Repeati609 – 652NHL 4Add BLAST44
Repeati656 – 699NHL 5Add BLAST44
Repeati700 – 743NHL 6Add BLAST44

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni2 – 290Interaction with KIF21BBy similarityAdd BLAST289

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili153 – 224Sequence analysisAdd BLAST72

Domaini

The interaction with MYO5B is dependent upon its NHL repeats, which form a beta-propeller (NHL) domain containing six blades.By similarity

Sequence similaritiesi

Belongs to the TRIM/RBCC family.Curated

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri22 – 63RING-typePROSITE-ProRule annotationAdd BLAST42
Zinc fingeri110 – 151B box-typePROSITE-ProRule annotationAdd BLAST42

Keywords - Domaini

Coiled coil, Repeat, Zinc-finger

Phylogenomic databases

eggNOGiENOG410ITF9 Eukaryota
ENOG410ZAM4 LUCA
GeneTreeiENSGT00920000148978
HOGENOMiHOG000220817
HOVERGENiHBG054843
InParanoidiO75382
KOiK11997
OMAiCLDRYRC
OrthoDBiEOG091G06IK
PhylomeDBiO75382
TreeFamiTF331018

Family and domain databases

CDDicd00021 BBOX, 1 hit
Gene3Di2.120.10.30, 2 hits
2.60.40.10, 1 hit
3.30.40.10, 1 hit
InterProiView protein in InterPro
IPR011042 6-blade_b-propeller_TolB-like
IPR003649 Bbox_C
IPR017868 Filamin/ABP280_repeat-like
IPR001298 Filamin/ABP280_rpt
IPR013783 Ig-like_fold
IPR014756 Ig_E-set
IPR001258 NHL_repeat
IPR013017 NHL_repeat_subgr
IPR027370 Znf-RING_LisH
IPR000315 Znf_B-box
IPR001841 Znf_RING
IPR013083 Znf_RING/FYVE/PHD
IPR017907 Znf_RING_CS
PfamiView protein in Pfam
PF00630 Filamin, 1 hit
PF01436 NHL, 6 hits
PF00643 zf-B_box, 1 hit
PF13445 zf-RING_UBOX, 1 hit
SMARTiView protein in SMART
SM00502 BBC, 1 hit
SM00336 BBOX, 1 hit
SM00557 IG_FLMN, 1 hit
SM00184 RING, 1 hit
SUPFAMiSSF81296 SSF81296, 1 hit
PROSITEiView protein in PROSITE
PS50194 FILAMIN_REPEAT, 1 hit
PS51125 NHL, 6 hits
PS50119 ZF_BBOX, 1 hit
PS00518 ZF_RING_1, 1 hit
PS50089 ZF_RING_2, 1 hit

Sequences (4+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 4 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 4 described isoforms and 3 potential isoforms that are computationally mapped.Show allAlign All

Isoform Alpha (identifier: O75382-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MAKREDSPGP EVQPMDKQFL VCSICLDRYQ CPKVLPCLHT FCERCLQNYI
60 70 80 90 100
PAQSLTLSCP VCRQTSILPE QGVSALQNNF FISSLMEAMQ QAPDGAHDPE
110 120 130 140 150
DPHPLSVVAG RPLSCPNHEG KTMEFYCEAC ETAMCGECRA GEHREHGTVL
160 170 180 190 200
LRDVVEQHKA ALQRQLEAVR GRLPQLSAAI ALVGGISQQL QERKAEALAQ
210 220 230 240 250
ISAAFEDLEQ ALQQRKQALV SDLETICGAK QKVLQSQLDT LRQGQEHIGS
260 270 280 290 300
SCSFAEQALR LGSAPEVLLV RKHMRERLAA LAAQAFPERP HENAQLELVL
310 320 330 340 350
EVDGLRRSVL NLGALLTTSA TAHETVATGE GLRQALVGQP ASLTVTTKDK
360 370 380 390 400
DGRLVRTGSA ELRAEITGPD GTRLPVPVVD HKNGTYELVY TARTEGELLL
410 420 430 440 450
SVLLYGQPVR GSPFRVRALR PGDLPPSPDD VKRRVKSPGG PGSHVRQKAV
460 470 480 490 500
RRPSSMYSTG GKRKDNPIED ELVFRVGSRG REKGEFTNLQ GVSAASSGRI
510 520 530 540 550
VVADSNNQCI QVFSNEGQFK FRFGVRGRSP GQLQRPTGVA VDTNGDIIVA
560 570 580 590 600
DYDNRWVSIF SPEGKFKTKI GAGRLMGPKG VAVDRNGHII VVDNKSCCVF
610 620 630 640 650
TFQPNGKLVG RFGGRGATDR HFAGPHFVAV NNKNEIVVTD FHNHSVKVYS
660 670 680 690 700
ADGEFLFKFG SHGEGNGQFN APTGVAVDSN GNIIVADWGN SRIQVFDSSG
710 720 730 740
SFLSYINTSA EPLYGPQGLA LTSDGHVVVA DAGNHCFKAY RYLQ
Length:744
Mass (Da):80,830
Last modified:November 25, 2008 - v2
Checksum:iFF9829CB52314DD2
GO
Isoform Beta (identifier: O75382-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     146-156: Missing.

Show »
Length:733
Mass (Da):79,610
Checksum:iA31439D4CDEBA6B7
GO
Isoform Gamma (identifier: O75382-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     224-302: Missing.

Show »
Length:665
Mass (Da):72,101
Checksum:i640D87E904DBDA4E
GO
Isoform 4 (identifier: O75382-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-119: Missing.
     120-120: G → M

Note: No experimental confirmation available.
Show »
Length:625
Mass (Da):67,726
Checksum:i8813BB2C040EB51C
GO

Computationally mapped potential isoform sequencesi

There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
A0A1W2PPI7A0A1W2PPI7_HUMAN
Tripartite motif-containing protein...
TRIM3
703Annotation score:
E9PMK8E9PMK8_HUMAN
Tripartite motif-containing protein...
TRIM3
260Annotation score:
E9PMW5E9PMW5_HUMAN
Tripartite motif-containing protein...
TRIM3
96Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti113L → F in AAC24809 (PubMed:11170753).Curated1
Sequence conflicti113L → F in AAG53474 (PubMed:11331580).Curated1
Sequence conflicti113L → F in AAG53475 (PubMed:11331580).Curated1
Sequence conflicti113L → F in AAG53476 (PubMed:11331580).Curated1
Sequence conflicti236S → T in AAC24809 (PubMed:11170753).Curated1
Sequence conflicti236S → T in AAG53474 (PubMed:11331580).Curated1
Sequence conflicti236S → T in AAG53475 (PubMed:11331580).Curated1
Sequence conflicti347T → A in AAC24809 (PubMed:11170753).Curated1
Sequence conflicti347T → A in AAG53474 (PubMed:11331580).Curated1
Sequence conflicti347T → A in AAG53475 (PubMed:11331580).Curated1
Sequence conflicti347T → A in AAG53476 (PubMed:11331580).Curated1
Sequence conflicti450V → L in BAH12882 (PubMed:14702039).Curated1
Sequence conflicti631N → S in AAC24809 (PubMed:11170753).Curated1
Sequence conflicti631N → S in AAG53474 (PubMed:11331580).Curated1
Sequence conflicti631N → S in AAG53475 (PubMed:11331580).Curated1
Sequence conflicti631N → S in AAG53476 (PubMed:11331580).Curated1
Sequence conflicti636I → V in AAC24809 (PubMed:11170753).Curated1
Sequence conflicti636I → V in AAG53474 (PubMed:11331580).Curated1
Sequence conflicti636I → V in AAG53475 (PubMed:11331580).Curated1
Sequence conflicti636I → V in AAG53476 (PubMed:11331580).Curated1
Sequence conflicti646V → E in AAC24809 (PubMed:11170753).Curated1
Sequence conflicti646V → E in AAG53474 (PubMed:11331580).Curated1
Sequence conflicti646V → E in AAG53475 (PubMed:11331580).Curated1
Sequence conflicti646V → E in AAG53476 (PubMed:11331580).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_052124298L → R. Corresponds to variant dbSNP:rs10128723Ensembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0447801 – 119Missing in isoform 4. 1 PublicationAdd BLAST119
Alternative sequenceiVSP_044781120G → M in isoform 4. 1 Publication1
Alternative sequenceiVSP_005758146 – 156Missing in isoform Beta. 1 PublicationAdd BLAST11
Alternative sequenceiVSP_005759224 – 302Missing in isoform Gamma. 1 PublicationAdd BLAST79

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF045239 mRNA Translation: AAC24809.1
AF220020 mRNA Translation: AAG53474.1
AF220021 mRNA Translation: AAG53475.1
AF220022 mRNA Translation: AAG53476.1
AK291396 mRNA Translation: BAF84085.1
AK298843 mRNA Translation: BAH12882.1
AC084337 Genomic DNA No translation available.
CH471064 Genomic DNA Translation: EAW68709.1
BC096827 mRNA Translation: AAH96827.1
CCDSiCCDS58115.1 [O75382-4]
CCDS7764.1 [O75382-1]
RefSeqiNP_001234935.1, NM_001248006.1 [O75382-1]
NP_001234936.1, NM_001248007.1 [O75382-4]
NP_006449.2, NM_006458.3 [O75382-1]
NP_150594.2, NM_033278.3 [O75382-1]
XP_011518146.1, XM_011519844.1
XP_011518147.1, XM_011519845.2 [O75382-4]
XP_016872587.1, XM_017017098.1 [O75382-1]
XP_016872588.1, XM_017017099.1
UniGeneiHs.591992

Genome annotation databases

EnsembliENST00000345851; ENSP00000340797; ENSG00000110171 [O75382-1]
ENST00000359518; ENSP00000352508; ENSG00000110171 [O75382-1]
ENST00000525074; ENSP00000433102; ENSG00000110171 [O75382-1]
ENST00000536344; ENSP00000445460; ENSG00000110171 [O75382-4]
GeneIDi10612
KEGGihsa:10612
UCSCiuc001mdh.4 human [O75382-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF045239 mRNA Translation: AAC24809.1
AF220020 mRNA Translation: AAG53474.1
AF220021 mRNA Translation: AAG53475.1
AF220022 mRNA Translation: AAG53476.1
AK291396 mRNA Translation: BAF84085.1
AK298843 mRNA Translation: BAH12882.1
AC084337 Genomic DNA No translation available.
CH471064 Genomic DNA Translation: EAW68709.1
BC096827 mRNA Translation: AAH96827.1
CCDSiCCDS58115.1 [O75382-4]
CCDS7764.1 [O75382-1]
RefSeqiNP_001234935.1, NM_001248006.1 [O75382-1]
NP_001234936.1, NM_001248007.1 [O75382-4]
NP_006449.2, NM_006458.3 [O75382-1]
NP_150594.2, NM_033278.3 [O75382-1]
XP_011518146.1, XM_011519844.1
XP_011518147.1, XM_011519845.2 [O75382-4]
XP_016872587.1, XM_017017098.1 [O75382-1]
XP_016872588.1, XM_017017099.1
UniGeneiHs.591992

3D structure databases

ProteinModelPortaliO75382
SMRiO75382
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi115858, 23 interactors
CORUMiO75382
IntActiO75382, 22 interactors
MINTiO75382
STRINGi9606.ENSP00000340797

PTM databases

iPTMnetiO75382
PhosphoSitePlusiO75382

Polymorphism and mutation databases

BioMutaiTRIM3

Proteomic databases

EPDiO75382
MaxQBiO75382
PaxDbiO75382
PeptideAtlasiO75382
PRIDEiO75382
ProteomicsDBi49955
49956 [O75382-2]
49957 [O75382-3]

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000345851; ENSP00000340797; ENSG00000110171 [O75382-1]
ENST00000359518; ENSP00000352508; ENSG00000110171 [O75382-1]
ENST00000525074; ENSP00000433102; ENSG00000110171 [O75382-1]
ENST00000536344; ENSP00000445460; ENSG00000110171 [O75382-4]
GeneIDi10612
KEGGihsa:10612
UCSCiuc001mdh.4 human [O75382-1]

Organism-specific databases

CTDi10612
DisGeNETi10612
EuPathDBiHostDB:ENSG00000110171.18
GeneCardsiTRIM3
HGNCiHGNC:10064 TRIM3
HPAiHPA043396
HPA048233
MIMi605493 gene
neXtProtiNX_O75382
OpenTargetsiENSG00000110171
PharmGKBiPA35534
GenAtlasiSearch...

Phylogenomic databases

eggNOGiENOG410ITF9 Eukaryota
ENOG410ZAM4 LUCA
GeneTreeiENSGT00920000148978
HOGENOMiHOG000220817
HOVERGENiHBG054843
InParanoidiO75382
KOiK11997
OMAiCLDRYRC
OrthoDBiEOG091G06IK
PhylomeDBiO75382
TreeFamiTF331018

Enzyme and pathway databases

ReactomeiR-HSA-877300 Interferon gamma signaling

Miscellaneous databases

ChiTaRSiTRIM3 human
GeneWikiiTRIM3
GenomeRNAii10612
PROiPR:O75382
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000110171 Expressed in 192 organ(s), highest expression level in right hemisphere of cerebellum
CleanExiHS_TRIM3
ExpressionAtlasiO75382 baseline and differential
GenevisibleiO75382 HS

Family and domain databases

CDDicd00021 BBOX, 1 hit
Gene3Di2.120.10.30, 2 hits
2.60.40.10, 1 hit
3.30.40.10, 1 hit
InterProiView protein in InterPro
IPR011042 6-blade_b-propeller_TolB-like
IPR003649 Bbox_C
IPR017868 Filamin/ABP280_repeat-like
IPR001298 Filamin/ABP280_rpt
IPR013783 Ig-like_fold
IPR014756 Ig_E-set
IPR001258 NHL_repeat
IPR013017 NHL_repeat_subgr
IPR027370 Znf-RING_LisH
IPR000315 Znf_B-box
IPR001841 Znf_RING
IPR013083 Znf_RING/FYVE/PHD
IPR017907 Znf_RING_CS
PfamiView protein in Pfam
PF00630 Filamin, 1 hit
PF01436 NHL, 6 hits
PF00643 zf-B_box, 1 hit
PF13445 zf-RING_UBOX, 1 hit
SMARTiView protein in SMART
SM00502 BBC, 1 hit
SM00336 BBOX, 1 hit
SM00557 IG_FLMN, 1 hit
SM00184 RING, 1 hit
SUPFAMiSSF81296 SSF81296, 1 hit
PROSITEiView protein in PROSITE
PS50194 FILAMIN_REPEAT, 1 hit
PS51125 NHL, 6 hits
PS50119 ZF_BBOX, 1 hit
PS00518 ZF_RING_1, 1 hit
PS50089 ZF_RING_2, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiTRIM3_HUMAN
AccessioniPrimary (citable) accession number: O75382
Secondary accession number(s): B7Z5E6
, F5H2Q8, Q4V9L4, Q9C038, Q9C039
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 6, 2002
Last sequence update: November 25, 2008
Last modified: October 10, 2018
This is version 172 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  2. SIMILARITY comments
    Index of protein domains and families
  3. Human chromosome 11
    Human chromosome 11: entries, gene names and cross-references to MIM
  4. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  5. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
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