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Protein

Vesicle-associated membrane protein 4

Gene

VAMP4

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Involved in the pathway that functions to remove an inhibitor (probably synaptotagmin-4) of calcium-triggered exocytosis during the maturation of secretory granules. May be a marker for this sorting pathway that is critical for remodeling the secretory response of granule.

GO - Biological processi

  • Golgi ribbon formation Source: UniProtKB
  • membrane organization Source: Reactome
  • microtubule cytoskeleton organization Source: UniProtKB
  • regulation of synaptic vesicle endocytosis Source: Ensembl
  • SNARE complex assembly Source: BHF-UCL
  • synaptic vesicle to endosome fusion Source: Ensembl

Enzyme and pathway databases

ReactomeiR-HSA-6811440 Retrograde transport at the Trans-Golgi-Network
R-HSA-8856825 Cargo recognition for clathrin-mediated endocytosis
R-HSA-8856828 Clathrin-mediated endocytosis
SIGNORiO75379

Names & Taxonomyi

Protein namesi
Recommended name:
Vesicle-associated membrane protein 4
Short name:
VAMP-4
Gene namesi
Name:VAMP4
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 1

Organism-specific databases

EuPathDBiHostDB:ENSG00000117533.14
HGNCiHGNC:12645 VAMP4
MIMi606909 gene
neXtProtiNX_O75379

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 115CytoplasmicSequence analysisAdd BLAST115
Transmembranei116 – 136Helical; Anchor for type IV membrane proteinSequence analysisAdd BLAST21
Topological domaini137 – 141VesicularSequence analysis5

Keywords - Cellular componenti

Golgi apparatus, Membrane

Pathology & Biotechi

Organism-specific databases

DisGeNETi8674
OpenTargetsiENSG00000117533
PharmGKBiPA37269

Polymorphism and mutation databases

BioMutaiVAMP4

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002067311 – 141Vesicle-associated membrane protein 4Add BLAST141

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei17PhosphoserineBy similarity1
Modified residuei30PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiO75379
PaxDbiO75379
PeptideAtlasiO75379
PRIDEiO75379
ProteomicsDBi49950
49951 [O75379-2]

PTM databases

iPTMnetiO75379
PhosphoSitePlusiO75379
SwissPalmiO75379

Expressioni

Gene expression databases

BgeeiENSG00000117533 Expressed in 238 organ(s), highest expression level in Brodmann (1909) area 23
CleanExiHS_VAMP4
ExpressionAtlasiO75379 baseline and differential
GenevisibleiO75379 HS

Organism-specific databases

HPAiHPA050418

Interactioni

Subunit structurei

Identified in a complex containing STX6, STX12, VAMP4 and VTI1A (By similarity). Interacts with BAIAP3; this interaction is increased in the presence of calcium (PubMed:28626000).By similarity1 Publication

Binary interactionsi

Protein-protein interaction databases

BioGridi114222, 52 interactors
CORUMiO75379
DIPiDIP-44226N
IntActiO75379, 56 interactors
MINTiO75379
STRINGi9606.ENSP00000236192

Structurei

3D structure databases

ProteinModelPortaliO75379
SMRiO75379
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini52 – 112v-SNARE coiled-coil homologyPROSITE-ProRule annotationAdd BLAST61

Sequence similaritiesi

Belongs to the synaptobrevin family.Curated

Keywords - Domaini

Coiled coil, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0860 Eukaryota
COG5143 LUCA
GeneTreeiENSGT00550000074449
HOGENOMiHOG000042711
HOVERGENiHBG061695
InParanoidiO75379
KOiK08513
OMAiWRGCKMK
OrthoDBiEOG091G0MPE
PhylomeDBiO75379
TreeFamiTF313666

Family and domain databases

InterProiView protein in InterPro
IPR001388 Synaptobrevin
IPR016444 Synaptobrevin/VAMP
PfamiView protein in Pfam
PF00957 Synaptobrevin, 1 hit
PIRSFiPIRSF005409 Synaptobrevin_euk, 1 hit
PRINTSiPR00219 SYNAPTOBREVN
PROSITEiView protein in PROSITE
PS00417 SYNAPTOBREVIN, 1 hit
PS50892 V_SNARE, 1 hit

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket
Isoform 1 (identifier: O75379-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MPPKFKRHLN DDDVTGSVKS ERRNLLEDDS DEEEDFFLRG PSGPRFGPRN
60 70 80 90 100
DKIKHVQNQV DEVIDVMQEN ITKVIERGER LDELQDKSES LSDNATAFSN
110 120 130 140
RSKQLRRQMW WRGCKIKAIM ALVAAILLLV IIILIVMKYR T
Length:141
Mass (Da):16,397
Last modified:April 3, 2002 - v2
Checksum:i556CC682D26CF91E
GO
Isoform 2 (identifier: O75379-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     39-39: Missing.

Note: No experimental confirmation available.
Show »
Length:140
Mass (Da):16,241
Checksum:iA2D4E66093D57837
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti68Q → P in AAC24032 (PubMed:9553086).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_00632639Missing in isoform 2. 1 Publication1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF044310 mRNA Translation: AAC24032.1
AL035296 mRNA Translation: CAA22896.1
Z98751 Genomic DNA No translation available.
CH471067 Genomic DNA Translation: EAW90904.1
BC005974 mRNA Translation: AAH05974.1
BC007019 mRNA Translation: AAH07019.1
BC031264 mRNA Translation: AAH31264.1
CCDSiCCDS1298.1 [O75379-1]
CCDS53430.1 [O75379-2]
RefSeqiNP_001172056.1, NM_001185127.1 [O75379-2]
NP_003753.2, NM_003762.4 [O75379-1]
UniGeneiHs.6651

Genome annotation databases

EnsembliENST00000236192; ENSP00000236192; ENSG00000117533 [O75379-1]
ENST00000367740; ENSP00000356714; ENSG00000117533 [O75379-2]
ENST00000474047; ENSP00000435933; ENSG00000117533 [O75379-1]
GeneIDi8674
KEGGihsa:8674
UCSCiuc001ghy.3 human [O75379-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF044310 mRNA Translation: AAC24032.1
AL035296 mRNA Translation: CAA22896.1
Z98751 Genomic DNA No translation available.
CH471067 Genomic DNA Translation: EAW90904.1
BC005974 mRNA Translation: AAH05974.1
BC007019 mRNA Translation: AAH07019.1
BC031264 mRNA Translation: AAH31264.1
CCDSiCCDS1298.1 [O75379-1]
CCDS53430.1 [O75379-2]
RefSeqiNP_001172056.1, NM_001185127.1 [O75379-2]
NP_003753.2, NM_003762.4 [O75379-1]
UniGeneiHs.6651

3D structure databases

ProteinModelPortaliO75379
SMRiO75379
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi114222, 52 interactors
CORUMiO75379
DIPiDIP-44226N
IntActiO75379, 56 interactors
MINTiO75379
STRINGi9606.ENSP00000236192

PTM databases

iPTMnetiO75379
PhosphoSitePlusiO75379
SwissPalmiO75379

Polymorphism and mutation databases

BioMutaiVAMP4

Proteomic databases

EPDiO75379
PaxDbiO75379
PeptideAtlasiO75379
PRIDEiO75379
ProteomicsDBi49950
49951 [O75379-2]

Protocols and materials databases

DNASUi8674
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000236192; ENSP00000236192; ENSG00000117533 [O75379-1]
ENST00000367740; ENSP00000356714; ENSG00000117533 [O75379-2]
ENST00000474047; ENSP00000435933; ENSG00000117533 [O75379-1]
GeneIDi8674
KEGGihsa:8674
UCSCiuc001ghy.3 human [O75379-1]

Organism-specific databases

CTDi8674
DisGeNETi8674
EuPathDBiHostDB:ENSG00000117533.14
GeneCardsiVAMP4
HGNCiHGNC:12645 VAMP4
HPAiHPA050418
MIMi606909 gene
neXtProtiNX_O75379
OpenTargetsiENSG00000117533
PharmGKBiPA37269
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG0860 Eukaryota
COG5143 LUCA
GeneTreeiENSGT00550000074449
HOGENOMiHOG000042711
HOVERGENiHBG061695
InParanoidiO75379
KOiK08513
OMAiWRGCKMK
OrthoDBiEOG091G0MPE
PhylomeDBiO75379
TreeFamiTF313666

Enzyme and pathway databases

ReactomeiR-HSA-6811440 Retrograde transport at the Trans-Golgi-Network
R-HSA-8856825 Cargo recognition for clathrin-mediated endocytosis
R-HSA-8856828 Clathrin-mediated endocytosis
SIGNORiO75379

Miscellaneous databases

ChiTaRSiVAMP4 human
GeneWikiiVAMP4
GenomeRNAii8674
PROiPR:O75379
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000117533 Expressed in 238 organ(s), highest expression level in Brodmann (1909) area 23
CleanExiHS_VAMP4
ExpressionAtlasiO75379 baseline and differential
GenevisibleiO75379 HS

Family and domain databases

InterProiView protein in InterPro
IPR001388 Synaptobrevin
IPR016444 Synaptobrevin/VAMP
PfamiView protein in Pfam
PF00957 Synaptobrevin, 1 hit
PIRSFiPIRSF005409 Synaptobrevin_euk, 1 hit
PRINTSiPR00219 SYNAPTOBREVN
PROSITEiView protein in PROSITE
PS00417 SYNAPTOBREVIN, 1 hit
PS50892 V_SNARE, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiVAMP4_HUMAN
AccessioniPrimary (citable) accession number: O75379
Secondary accession number(s): A2IDD8
, Q96IY9, Q96J20, Q9UEL7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 21, 2001
Last sequence update: April 3, 2002
Last modified: November 7, 2018
This is version 170 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Human chromosome 1
    Human chromosome 1: entries, gene names and cross-references to MIM
  3. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
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Main funding by: National Institutes of Health

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