H2AFY

Functionⁱ

Variant histone H2A which replaces conventional H2A in a subset of nucleosomes where it represses transcription (PubMed:12718888, PubMed:15621527, PubMed:16428466). Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Involved in stable X chromosome inactivation (PubMed:15897469). Inhibits the binding of transcription factors, including NF-kappa-B, and interferes with the activity of remodeling SWI/SNF complexes (PubMed:12718888, PubMed:16428466). Inhibits histone acetylation by EP300 and recruits class I HDACs, which induces a hypoacetylated state of chromatin (PubMed:16428466, PubMed:16107708).5 Publications

Isoform 1: Binds ADP-ribose and O-acetyl-ADP-ribose, and may be involved in ADP-ribose-mediated chromatin modulation (PubMed:15902274). Increases the expression of genes involved in redox metabolism, including SOD3 (PubMed:23022728).2 Publications

Isoform 2: Represses SOD3 gene expression.1 Publication

GO - Molecular functionⁱ

chromatin DNA binding
Source: UniProtKB
Inferred from direct assayⁱ
- 19734898
- 24071584
DNA binding
Source: GO_Central
Inferred from biological aspect of ancestorⁱ
- 21873635
double-stranded methylated DNA binding
Source: UniProtKB
Inferred from direct assayⁱ
- 24071584
enzyme binding
Source: BHF-UCL
Inferred from physical interactionⁱ
- 26373281
nucleosomal DNA binding
Source: UniProtKB
Inferred from direct assayⁱ
- 20008927
promoter-specific chromatin binding Source: UniProtKB
protein heterodimerization activity Source: InterPro
protein kinase binding
Source: UniProtKB
Inferred from physical interactionⁱ
- 22194607
protein serine/threonine kinase inhibitor activity
Source: UniProtKB
Inferred from mutant phenotypeⁱ
- 22194607
rDNA binding
Source: UniProtKB
Inferred from direct assayⁱ
- 24071584
RNA polymerase II core promoter sequence-specific DNA binding
Source: UniProtKB
Inferred from direct assayⁱ
- 25526730
RNA polymerase II regulatory region sequence-specific DNA binding
Source: UniProtKB
Inferred from direct assayⁱ
- 23595991
transcription regulatory region DNA binding
Source: UniProtKB
Inferred from direct assayⁱ
- 19734898
- 20008927

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

chromatin organization
Source: GO_Central
Inferred from biological aspect of ancestorⁱ
- 21873635
dosage compensation
Source: MGI
Inferred from direct assayⁱ
- 15053874
establishment of protein localization to chromatin
Source: UniProtKB
Inferred from mutant phenotypeⁱ
- 19734898
negative regulation of cell cycle G2/M phase transition
Source: UniProtKB
Inferred from mutant phenotypeⁱ
- 22194607
negative regulation of gene expression, epigenetic
Source: UniProtKB
Inferred from mutant phenotypeⁱ
negative regulation of histone H3-K27 methylation
Source: UniProtKB
Inferred from mutant phenotypeⁱ
- 25526730
negative regulation of histone H3-K4 methylation
Source: UniProtKB
Inferred from mutant phenotypeⁱ
- 25526730
negative regulation of histone phosphorylation
Source: UniProtKB
Inferred from mutant phenotypeⁱ
- 22194607
negative regulation of protein localization to chromosome, telomeric region
Source: BHF-UCL
Inferred from direct assayⁱ
- 26373281
negative regulation of protein serine/threonine kinase activity
Source: UniProtKB
Inferred from mutant phenotypeⁱ
- 22194607
negative regulation of response to oxidative stress
Source: UniProtKB
Inferred from mutant phenotypeⁱ
- 23022728
negative regulation of transcription by RNA polymerase II
Source: UniProtKB
Inferred from mutant phenotypeⁱ
- 19734898
negative regulation of transcription of nucleolar large rRNA by RNA polymerase I
Source: UniProtKB
Inferred from mutant phenotypeⁱ
- 24071584
nucleosome assembly
Source: UniProtKB
Non-traceable author statementⁱ
- 9714746
positive regulation of gene expression, epigenetic
Source: UniProtKB
Inferred from mutant phenotypeⁱ
- 25526730
positive regulation of keratinocyte differentiation
Source: UniProtKB
Inferred from mutant phenotypeⁱ
- 23595991
positive regulation of maintenance of mitotic sister chromatid cohesion
Source: BHF-UCL
Inferred from direct assayⁱ
- 26373281
positive regulation of response to oxidative stress
Source: UniProtKB
Inferred from mutant phenotypeⁱ
- 23022728
regulation of gene expression, epigenetic
Source: UniProtKB
Inferred from mutant phenotypeⁱ
- 20008927
regulation of lipid metabolic process
Source: UniProtKB
Inferred from mutant phenotypeⁱ
- 25526730
regulation of response to oxidative stress
Source: UniProtKB
Inferred from mutant phenotypeⁱ
- 23022728

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Molecular function	Chromatin regulator, DNA-binding

Molecular function

Chromatin regulator, DNA-binding

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Core histone macro-H2A.1 Short name: Histone macroH2A1 Short name: mH2A1 Alternative name(s): Histone H2A.y Short name: H2A/y Medulloblastoma antigen MU-MB-50.205
Gene namesⁱ	Name:H2AFY Synonyms:MACROH2A1
Organismⁱ	Homo sapiens (Human)
Taxonomic identifierⁱ	9606 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Dipnotetrapodomorpha › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Boreoeutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo
Proteomesⁱ	UP000005640 Componentⁱ: Chromosome 5

Organism-specific databases

EuPathDBⁱ	HostDB:ENSG00000113648.16
Human Gene Nomenclature Database More...HGNCⁱ	HGNC:4740 H2AFY
MIMⁱ	610054 gene
neXtProtⁱ	NX_O75367

Keywords - Cellular componentⁱ

Chromosome, Nucleosome core, Nucleus

Pathology & Biotechⁱ

Organism-specific databases

DisGeNET More...DisGeNETⁱ	9555
Open Targets More...OpenTargetsⁱ	ENSG00000113648
PharmGKBⁱ	PA29117

Polymorphism and mutation databases

BioMutaⁱ	H2AFY

BioMutaⁱ

H2AFY

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
ChainⁱPRO_0000055318	1 – 372	Core histone macro-H2A.1Add BLAST		372

Amino acid modifications

Feature key	Position(s)	DescriptionActions	Length
Modified residueⁱ	18	N6-methyllysine1 Publication	1
Modified residueⁱ	116	N6-acetyllysine; alternateBy similarity	1
Cross-linkⁱ	116	Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin); alternate1 Publication
Cross-linkⁱ	117	Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)1 Publication
Modified residueⁱ	123	N6,N6-dimethyllysine; alternate1 Publication	1
Modified residueⁱ	123	N6-acetyllysine; alternateBy similarity	1
Cross-linkⁱ	123	Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2); alternateCombined sources
Modified residueⁱ	129	PhosphothreonineCombined sources1 Publication	1
Cross-linkⁱ	167	Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Modified residueⁱ	170	PhosphoserineCombined sources	1
Modified residueⁱ	173	PhosphoserineCombined sources	1
Modified residueⁱ	178	PhosphothreonineCombined sources	1
Cross-linkⁱ	189	Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Cross-linkⁱ	323	Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources

Post-translational modificationⁱ

Monoubiquitinated at either Lys-116 or Lys-117. May also be polyubiquitinated. Ubiquitination is mediated by the CUL3/SPOP E3 complex and does not promote proteasomal degradation. Instead, it is required for enrichment in inactive X chromosome chromatin.3 Publications

Keywords - PTMⁱ

Acetylation, Isopeptide bond, Methylation, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDⁱ	O75367
MaxQB - The MaxQuant DataBase More...MaxQBⁱ	O75367
PaxDbⁱ	O75367
PeptideAtlas More...PeptideAtlasⁱ	O75367
PRIDEⁱ	O75367
ProteomicsDBⁱ	49934 49935 [O75367-2] 49936 [O75367-3]
TopDownProteomicsⁱ	O75367-1 [O75367-1] O75367-2 [O75367-2]

2D gel databases

SWISS-2DPAGEⁱ	O75367

SWISS-2DPAGEⁱ

O75367

PTM databases

iPTMnetⁱ	O75367
PhosphoSitePlusⁱ	O75367
SwissPalmⁱ	O75367

Miscellaneous databases

PMAP-CutDBⁱ	O75367

PMAP-CutDBⁱ

O75367

Expressionⁱ

Tissue specificityⁱ

Widely expressed.1 Publication

Gene expression databases

Bgeeⁱ	ENSG00000113648 Expressed in 236 organ(s), highest expression level in vagina
CleanExⁱ	HS_H2AFY
ExpressionAtlasⁱ	O75367 baseline and differential
Genevisibleⁱ	O75367 HS

Organism-specific databases

Human Protein Atlas More...HPAⁱ	HPA041189 HPA050962

HPAⁱ

HPA041189
HPA050962

Interactionⁱ

Subunit structureⁱ

The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. Interacts with HDAC1 and HDAC2 (By similarity). Interacts with SPOP. Part of a complex consisting of H2AFY, CUL3 and SPOP.By similarity3 Publications

Binary interactionsⁱ

With	Entry	#Exp.	IntAct
ATRX	P46100	2	EBI-6249599,EBI-396461
ERBB2	P04626	6	EBI-2868511,EBI-641062
ERICH2	A1L162	5	EBI-2868511,EBI-2682520
HIST1H2BN	U3KQK0	4	EBI-2868511,EBI-12142839
MAX	P61244	2	EBI-2868511,EBI-751711
TNKS	O95271	6	EBI-6249599,EBI-1105254

GO - Molecular functionⁱ

enzyme binding
Source: BHF-UCL
Inferred from physical interactionⁱ
- 26373281
protein heterodimerization activity Source: InterPro
protein kinase binding
Source: UniProtKB
Inferred from physical interactionⁱ
- 22194607

View the complete GO annotation on QuickGO ...

Protein-protein interaction databases

BioGridⁱ	114927, 96 interactors
CORUMⁱ	O75367
Database of interacting proteins More...DIPⁱ	DIP-44283N
ELMⁱ	O75367
IntActⁱ	O75367, 43 interactors
Molecular INTeraction database More...MINTⁱ	O75367
STRINGⁱ	9606.ENSP00000423563

Structureⁱ

Secondary structure

Legend: HelixTurnBeta strandPDB Structure known for this area

Feature key	Position(s)	DescriptionActions	Length
Helixⁱ	15 – 19	Combined sources	5
Helixⁱ	25 – 35	Combined sources	11
Beta strandⁱ	36 – 38	Combined sources	3
Beta strandⁱ	39 – 41	Combined sources	3
Helixⁱ	44 – 70	Combined sources	27
Beta strandⁱ	74 – 76	Combined sources	3
Helixⁱ	78 – 86	Combined sources	9
Helixⁱ	89 – 94	Combined sources	6
Turnⁱ	95 – 97	Combined sources	3
Beta strandⁱ	98 – 100	Combined sources	3
Helixⁱ	111 – 113	Combined sources	3
Beta strandⁱ	174 – 177	Combined sources	4
Beta strandⁱ	185 – 190	Combined sources	6
Beta strandⁱ	196 – 202	Combined sources	7
Helixⁱ	204 – 209	Combined sources	6
Beta strandⁱ	213 – 219	Combined sources	7
Helixⁱ	227 – 252	Combined sources	26
Beta strandⁱ	260 – 264	Combined sources	5
Beta strandⁱ	268 – 277	Combined sources	10
Helixⁱ	286 – 303	Combined sources	18
Beta strandⁱ	307 – 311	Combined sources	5
Beta strandⁱ	315 – 319	Combined sources	5
Helixⁱ	323 – 340	Combined sources	18
Beta strandⁱ	341 – 343	Combined sources	3
Beta strandⁱ	348 – 355	Combined sources	8
Helixⁱ	356 – 367	Combined sources	12

Show more details

3D structure databases

ProteinModelPortalⁱ	O75367
SMRⁱ	O75367
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Miscellaneous databases

EvolutionaryTraceⁱ	O75367

EvolutionaryTraceⁱ

O75367

Family & Domainsⁱ

Domains and Repeats

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Domainⁱ	2 – 117	Histone H2AAdd BLAST		116
Domainⁱ	184 – 370	MacroPROSITE-ProRule annotationAdd BLAST		187

Compositional bias

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Compositional biasⁱ	118 – 162	Lys-richAdd BLAST		45

Phylogenomic databases

eggNOGⁱ	ENOG410IV3A Eukaryota KOG1756 Eukaryota KOG2633 Eukaryota COG2110 LUCA COG5262 LUCA
Ensembl GeneTree More...GeneTreeⁱ	ENSGT00910000143981
HOVERGENⁱ	HBG009342
InParanoidⁱ	O75367
KEGG Orthology (KO) More...KOⁱ	K11251
OMAⁱ	HSEVSNL
Database of Orthologous Groups More...OrthoDBⁱ	EOG091G0XGD
PhylomeDBⁱ	O75367
TreeFamⁱ	TF332276

Family and domain databases

Conserved Domains Database More...CDDⁱ	cd00074 H2A, 1 hit cd02904 Macro_H2A_like, 1 hit
Gene3Dⁱ	1.10.20.10, 1 hit
InterProⁱ	View protein in InterPro IPR021171 Core_histone_macro-H2A IPR009072 Histone-fold IPR002119 Histone_H2A IPR007125 Histone_H2A/H2B/H3 IPR032454 Histone_H2A_C IPR002589 Macro_dom IPR035796 Macro_H2A
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF00125 Histone, 1 hit PF16211 Histone_H2A_C, 1 hit PF01661 Macro, 1 hit
PIRSFⁱ	PIRSF037942 Core_histone_macro-H2A, 1 hit
PRINTSⁱ	PR00620 HISTONEH2A
SMARTⁱ	View protein in SMART SM00506 A1pp, 1 hit SM00414 H2A, 1 hit
SUPFAMⁱ	SSF47113 SSF47113, 1 hit
PROSITEⁱ	View protein in PROSITE PS51154 MACRO, 1 hit

Sequences (3+)ⁱ

Sequence statusⁱ: Complete.

This entry describes 3 isoformsⁱ produced by alternative splicing. Align Add to basket

This entry has 3 described isoforms and 2 potential isoforms that are computationally mapped.Show all Align All

Isoform 2 (identifier: O75367-1) [UniParc]FASTA Add to basket

Also known as: mH2A1.21 Publication

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSSRGGKKKS TKTSRSAKAG VIFPVGRMLR YIKKGHPKYR IGVGAPVYMA 
        60         70         80         90        100
AVLEYLTAEI LELAGNAARD NKKGRVTPRH ILLAVANDEE LNQLLKGVTI 
       110        120        130        140        150
ASGGVLPNIH PELLAKKRGS KGKLEAIITP PPAKKAKSPS QKKPVSKKAG 
       160        170        180        190        200
GKKGARKSKK KQGEVSKAAS ADSTTEGTPA DGFTVLSTKS LFLGQKLNLI 
       210        220        230        240        250
HSEISNLAGF EVEAIINPTN ADIDLKDDLG NTLEKKGGKE FVEAVLELRK 
       260        270        280        290        300
KNGPLEVAGA AVSAGHGLPA KFVIHCNSPV WGADKCEELL EKTVKNCLAL 
       310        320        330        340        350
ADDKKLKSIA FPSIGSGRNG FPKQTAAQLI LKAISSYFVS TMSSSIKTVY 
       360        370 
FVLFDSESIG IYVQEMAKLD AN

Note: The preferential expression of isoform 2 over that of isoform 1 requires the presence of DDX5/DDX17.1 Publication

Length:372

Mass (Da):39,617

Last modified:January 23, 2007 - v4

Checksum:ⁱ37DED989EF7E69DC

GO

Isoform 1 (identifier: O75367-2) [UniParc]FASTA Add to basket

Also known as: mH2A1.11 Publication

The sequence of this isoform differs from the canonical sequence as follows:
198-229: NLIHSEISNLAGFEVEAIINPTNADIDLKDDL → QVVQADIASIDSDAVVHPTNTDFYIGGEV

« Hide

        10         20         30         40         50
MSSRGGKKKS TKTSRSAKAG VIFPVGRMLR YIKKGHPKYR IGVGAPVYMA 
        60         70         80         90        100
AVLEYLTAEI LELAGNAARD NKKGRVTPRH ILLAVANDEE LNQLLKGVTI 
       110        120        130        140        150
ASGGVLPNIH PELLAKKRGS KGKLEAIITP PPAKKAKSPS QKKPVSKKAG 
       160        170        180        190        200
GKKGARKSKK KQGEVSKAAS ADSTTEGTPA DGFTVLSTKS LFLGQKLQVV 
       210        220        230        240        250
QADIASIDSD AVVHPTNTDF YIGGEVGNTL EKKGGKEFVE AVLELRKKNG 
       260        270        280        290        300
PLEVAGAAVS AGHGLPAKFV IHCNSPVWGA DKCEELLEKT VKNCLALADD 
       310        320        330        340        350
KKLKSIAFPS IGSGRNGFPK QTAAQLILKA ISSYFVSTMS SSIKTVYFVL 
       360 
FDSESIGIYV QEMAKLDAN

Note: Specifically binds ADP-ribose and O-acetyl-ADP-ribose. Important residues for binding are Asp-203, Gly-224, Gly-314 and Phe-348. Preferentially expressed over isoform 2 in the absence of DDX5/DDX17.2 Publications

Show »

Length:369

Mass (Da):39,184

Checksum:ⁱD21A4CE6C7AA3BB1

GO

Isoform 3 (identifier: O75367-3) [UniParc]FASTA Add to basket

The sequence of this isoform differs from the canonical sequence as follows:
159-159: Missing.

« Hide

        10         20         30         40         50
MSSRGGKKKS TKTSRSAKAG VIFPVGRMLR YIKKGHPKYR IGVGAPVYMA 
        60         70         80         90        100
AVLEYLTAEI LELAGNAARD NKKGRVTPRH ILLAVANDEE LNQLLKGVTI 
       110        120        130        140        150
ASGGVLPNIH PELLAKKRGS KGKLEAIITP PPAKKAKSPS QKKPVSKKAG 
       160        170        180        190        200
GKKGARKSKK QGEVSKAASA DSTTEGTPAD GFTVLSTKSL FLGQKLNLIH 
       210        220        230        240        250
SEISNLAGFE VEAIINPTNA DIDLKDDLGN TLEKKGGKEF VEAVLELRKK 
       260        270        280        290        300
NGPLEVAGAA VSAGHGLPAK FVIHCNSPVW GADKCEELLE KTVKNCLALA 
       310        320        330        340        350
DDKKLKSIAF PSIGSGRNGF PKQTAAQLIL KAISSYFVST MSSSIKTVYF 
       360        370 
VLFDSESIGI YVQEMAKLDA N

Show »

Length:371

Mass (Da):39,489

Checksum:ⁱ54320BFD118454D1

GO

Computationally mapped potential isoform sequencesⁱ

There are 2 potential isoforms mapped to this entry.BLAST Align Show all Add to basket

Entry	Entry name	Protein names	Gene names	Length	Annotation

B4DJC3	B4DJC3_HUMAN	Histone H2A Histone H2A	H2AFY	200	Annotation score:
D6RCF2	D6RCF2_HUMAN	Histone H2A Histone H2A	H2AFY	165	Annotation score:

Experimental Info

Feature key	Position(s)	DescriptionActions	Length
Sequence conflictⁱ	186	L → F in BAB14565 (PubMed:14702039).Curated	1
Sequence conflictⁱ	210	F → S in BAB14565 (PubMed:14702039).Curated	1
Sequence conflictⁱ	225	L → P in AAC33433 (PubMed:9714746).Curated	1
Sequence conflictⁱ	291	E → G in AAH95406 (PubMed:15489334).Curated	1

Alternative sequence

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Alternative sequenceⁱVSP_038379	159	Missing in isoform 3. 2 Publications		1
Alternative sequenceⁱVSP_002056	198 – 229	NLIHS…LKDDL → QVVQADIASIDSDAVVHPTN TDFYIGGEV in isoform 1. 1 PublicationAdd BLAST		32

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AF041483 mRNA Translation: AAC33433.1 AF044286 mRNA Translation: AAC33434.1 AF054174 mRNA Translation: AAC39908.1 AK023409 mRNA Translation: BAB14565.1 AC026691 Genomic DNA No translation available. BC013331 mRNA Translation: AAH13331.1 BC095406 mRNA Translation: AAH95406.1 AY134746 mRNA Translation: AAN08620.1
The Consensus CDS (CCDS) project More...CCDSⁱ	CCDS4183.1 [O75367-3] CCDS4184.1 [O75367-2] CCDS4185.1 [O75367-1]
NCBI Reference Sequences More...RefSeqⁱ	NP_001035248.1, NM_001040158.1 [O75367-3] NP_004884.1, NM_004893.2 [O75367-3] NP_613075.1, NM_138609.2 [O75367-2] NP_613258.2, NM_138610.2 [O75367-1] XP_011542031.1, XM_011543729.2 [O75367-1] XP_011542032.1, XM_011543730.2 [O75367-3]
UniGeneⁱ	Hs.420272 Hs.599225

Genome annotation databases

Ensemblⁱ	ENST00000304332; ENSP00000302572; ENSG00000113648 [O75367-3] ENST00000312469; ENSP00000310169; ENSG00000113648 [O75367-2] ENST00000510038; ENSP00000424971; ENSG00000113648 [O75367-1] ENST00000511689; ENSP00000423563; ENSG00000113648 [O75367-1]
GeneIDⁱ	9555
KEGGⁱ	hsa:9555
UCSC genome browser More...UCSCⁱ	uc003lam.2 human [O75367-1]

Keywords - Coding sequence diversityⁱ

Alternative splicing

Similar proteinsⁱ

100% Identity
90% Identity
50% Identity

Protein	Similar proteins		Species	Score	Length	Source
O75367	Core histone macro-H2A		MACMU		372	UniRef100_O75367
Core histone macro-H2A		PANTR		372
Core histone macro-H2A		CALJA		372
Core histone macro-H2A		DELLE		372
Uncharacterized protein (Fragment)		AILME		372
+23
O75367-2	Core histone macro-H2A		CALJA		369	UniRef100_F7IAS0
Core histone macro-H2A		PANTR		369
Core histone macro-H2A		PROCO		369
Core histone macro-H2A		SAIBB		369
Core histone macro-H2A		MACMU		369
+12
O75367-3	Core histone macro-H2A		CALJA		371	UniRef100_U3BNA1
Core histone macro-H2A		MACMU		371
Core histone macro-H2A		PANTR		371
Core histone macro-H2A		LIPVE		371
Core histone macro-H2A		ODORO		371
+12

Protein	Similar proteins		Species	Score	Length	Source
O75367	Core histone macro-H2A.1		CHICK		372	UniRef90_O75367
Core histone macro-H2A.1		MOUSE		372
Core histone macro-H2A.1	)	RAT		371
Core histone macro-H2A		MACMU		372
Core histone macro-H2A		PANTR		372
+432

Protein	Similar proteins		Species	Score	Length	Source
O75367	Core histone macro-H2A.1		CHICK		372	UniRef50_O75367
Core histone macro-H2A.1		MOUSE		372
Core histone macro-H2A.1	)	RAT		371
Core histone macro-H2A		MACMU		372
Core histone macro-H2A		PANTR		372
+510

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AF041483 mRNA Translation: AAC33433.1 AF044286 mRNA Translation: AAC33434.1 AF054174 mRNA Translation: AAC39908.1 AK023409 mRNA Translation: BAB14565.1 AC026691 Genomic DNA No translation available. BC013331 mRNA Translation: AAH13331.1 BC095406 mRNA Translation: AAH95406.1 AY134746 mRNA Translation: AAN08620.1
CCDSⁱ	CCDS4183.1 [O75367-3] CCDS4184.1 [O75367-2] CCDS4185.1 [O75367-1]
RefSeqⁱ	NP_001035248.1, NM_001040158.1 [O75367-3] NP_004884.1, NM_004893.2 [O75367-3] NP_613075.1, NM_138609.2 [O75367-2] NP_613258.2, NM_138610.2 [O75367-1] XP_011542031.1, XM_011543729.2 [O75367-1] XP_011542032.1, XM_011543730.2 [O75367-3]
UniGeneⁱ	Hs.420272 Hs.599225

3D structure databases

Select the link destinations: Protein Data Bank Europe More...PDBeⁱ Protein Data Bank RCSB More...RCSB PDBⁱ Protein Data Bank Japan More...PDBjⁱ Links Updated	PDB entry	Method	Resolution (Å)	Chain	Positions	PDBsum
	1U35	X-ray	3.00	C/G	1-120	[»]
	1ZR3	X-ray	1.66	A/B/C/D	162-372	[»]
	1ZR5	X-ray	2.92	A/B	161-372	[»]
	2F8N	X-ray	2.90	G	1-120	[»]
	2FXK	X-ray	2.54	A/B	162-372	[»]
	3HQH	X-ray	2.30	M	167-181	[»]
	3HSV	X-ray	1.43	M	172-186	[»]
	3IID	X-ray	1.90	A	162-372	[»]
	3IIF	X-ray	2.10	A/B/C	162-372	[»]
	3IVB	X-ray	1.75	M	167-181	[»]
	5IIT	X-ray	2.13	A/B/C/D	181-369	[»]
	5LNC	X-ray	3.29	A/B	182-369	[»]
ProteinModelPortalⁱ	O75367
SMRⁱ	O75367
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

BioGridⁱ	114927, 96 interactors
CORUMⁱ	O75367
DIPⁱ	DIP-44283N
ELMⁱ	O75367
IntActⁱ	O75367, 43 interactors
MINTⁱ	O75367
STRINGⁱ	9606.ENSP00000423563

PTM databases

iPTMnetⁱ	O75367
PhosphoSitePlusⁱ	O75367
SwissPalmⁱ	O75367

Polymorphism and mutation databases

BioMutaⁱ	H2AFY

BioMutaⁱ

H2AFY

2D gel databases

SWISS-2DPAGEⁱ	O75367

SWISS-2DPAGEⁱ

O75367

Proteomic databases

EPDⁱ	O75367
MaxQBⁱ	O75367
PaxDbⁱ	O75367
PeptideAtlasⁱ	O75367
PRIDEⁱ	O75367
ProteomicsDBⁱ	49934 49935 [O75367-2] 49936 [O75367-3]
TopDownProteomicsⁱ	O75367-1 [O75367-1] O75367-2 [O75367-2]

Protocols and materials databases

The DNASU plasmid repository More...DNASUⁱ	9555
Structural Biology Knowledgebase	Search...

Genome annotation databases

Ensemblⁱ	ENST00000304332; ENSP00000302572; ENSG00000113648 [O75367-3] ENST00000312469; ENSP00000310169; ENSG00000113648 [O75367-2] ENST00000510038; ENSP00000424971; ENSG00000113648 [O75367-1] ENST00000511689; ENSP00000423563; ENSG00000113648 [O75367-1]
GeneIDⁱ	9555
KEGGⁱ	hsa:9555
UCSCⁱ	uc003lam.2 human [O75367-1]

Organism-specific databases

CTDⁱ	9555
DisGeNETⁱ	9555
EuPathDBⁱ	HostDB:ENSG00000113648.16
GeneCardsⁱ	H2AFY
HGNCⁱ	HGNC:4740 H2AFY
HPAⁱ	HPA041189 HPA050962
MIMⁱ	610054 gene
neXtProtⁱ	NX_O75367
OpenTargetsⁱ	ENSG00000113648
PharmGKBⁱ	PA29117
GenAtlas: human gene database More...GenAtlasⁱ	Search...

Phylogenomic databases

eggNOGⁱ	ENOG410IV3A Eukaryota KOG1756 Eukaryota KOG2633 Eukaryota COG2110 LUCA COG5262 LUCA
GeneTreeⁱ	ENSGT00910000143981
HOVERGENⁱ	HBG009342
InParanoidⁱ	O75367
KOⁱ	K11251
OMAⁱ	HSEVSNL
OrthoDBⁱ	EOG091G0XGD
PhylomeDBⁱ	O75367
TreeFamⁱ	TF332276

Miscellaneous databases

ChiTaRSⁱ	H2AFY human
EvolutionaryTraceⁱ	O75367
GeneWikiⁱ	H2AFY
GenomeRNAiⁱ	9555
PMAP-CutDBⁱ	O75367
Protein Ontology More...PROⁱ	PR:O75367
SOURCEⁱ	Search...

Gene expression databases

Bgeeⁱ	ENSG00000113648 Expressed in 236 organ(s), highest expression level in vagina
CleanExⁱ	HS_H2AFY
ExpressionAtlasⁱ	O75367 baseline and differential
Genevisibleⁱ	O75367 HS

Family and domain databases

CDDⁱ	cd00074 H2A, 1 hit cd02904 Macro_H2A_like, 1 hit
Gene3Dⁱ	1.10.20.10, 1 hit
InterProⁱ	View protein in InterPro IPR021171 Core_histone_macro-H2A IPR009072 Histone-fold IPR002119 Histone_H2A IPR007125 Histone_H2A/H2B/H3 IPR032454 Histone_H2A_C IPR002589 Macro_dom IPR035796 Macro_H2A
Pfamⁱ	View protein in Pfam PF00125 Histone, 1 hit PF16211 Histone_H2A_C, 1 hit PF01661 Macro, 1 hit
PIRSFⁱ	PIRSF037942 Core_histone_macro-H2A, 1 hit
PRINTSⁱ	PR00620 HISTONEH2A
SMARTⁱ	View protein in SMART SM00506 A1pp, 1 hit SM00414 H2A, 1 hit
SUPFAMⁱ	SSF47113 SSF47113, 1 hit
PROSITEⁱ	View protein in PROSITE PS51154 MACRO, 1 hit
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	H2AY_HUMAN
Accessionⁱ	O75367Primary (citable) accession number: O75367 Secondary accession number(s): O75377 , Q503A8, Q7Z5E3, Q96D41, Q9H8P3, Q9UP96
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	January 24, 2001
	Last sequence update:	January 23, 2007
	Last modified:	November 7, 2018
	This is version 191 of the entry and version 4 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Chordata Protein Annotation Program
Disclaimer	Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousⁱ

Keywords - Technical termⁱ

3D-structure, Complete proteome, Reference proteome

Documents

MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
PDB cross-references
Index of Protein Data Bank (PDB) cross-references
Human chromosome 5
Human chromosome 5: entries, gene names and cross-references to MIM

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UniProtKB - O75367 (H2AY_HUMAN)

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Core histone macro-H2A.1

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<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

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Gene expression databases

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Keywords - Coding sequence diversityi

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Functionⁱ

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

Keywords - Cellular componentⁱ

Pathology & Biotechⁱ

PTM / Processingⁱ

Keywords - PTMⁱ

Expressionⁱ

Interactionⁱ

GO - Molecular functionⁱ

Structureⁱ

Family & Domainsⁱ

Keywords - Coding sequence diversityⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ

Keywords - Technical termⁱ