UniProtKB - O75367 (H2AY_HUMAN)
Core histone macro-H2A.1
MACROH2A1
Functioni
GO - Molecular functioni
- chromatin DNA binding Source: UniProtKB
- DNA binding Source: GO_Central
- double-stranded methylated DNA binding Source: UniProtKB
- enzyme binding Source: BHF-UCL
- nucleosomal DNA binding Source: UniProtKB
- promoter-specific chromatin binding Source: UniProtKB
- protein heterodimerization activity Source: InterPro
- protein kinase binding Source: UniProtKB
- protein serine/threonine kinase inhibitor activity Source: UniProtKB
- rDNA binding Source: UniProtKB
- RNA polymerase II core promoter sequence-specific DNA binding Source: UniProtKB
- RNA polymerase II transcription regulatory region sequence-specific DNA binding Source: UniProtKB
- transcription regulatory region sequence-specific DNA binding Source: UniProtKB
GO - Biological processi
- chromatin silencing Source: GO_Central
- dosage compensation Source: MGI
- establishment of protein localization to chromatin Source: UniProtKB
- negative regulation of cell cycle G2/M phase transition Source: UniProtKB
- negative regulation of gene expression, epigenetic Source: UniProtKB
- negative regulation of histone H3-K27 methylation Source: UniProtKB
- negative regulation of histone H3-K4 methylation Source: UniProtKB
- negative regulation of histone phosphorylation Source: UniProtKB
- negative regulation of protein localization to chromosome, telomeric region Source: BHF-UCL
- negative regulation of protein serine/threonine kinase activity Source: UniProtKB
- negative regulation of response to oxidative stress Source: UniProtKB
- negative regulation of transcription by RNA polymerase II Source: UniProtKB
- negative regulation of transcription of nucleolar large rRNA by RNA polymerase I Source: UniProtKB
- nucleosome assembly Source: UniProtKB
- positive regulation of endodermal cell differentiation Source: UniProtKB
- positive regulation of gene expression, epigenetic Source: UniProtKB
- positive regulation of keratinocyte differentiation Source: UniProtKB
- positive regulation of maintenance of mitotic sister chromatid cohesion Source: BHF-UCL
- positive regulation of response to oxidative stress Source: UniProtKB
- regulation of gene expression, epigenetic Source: UniProtKB
- regulation of lipid metabolic process Source: UniProtKB
- regulation of response to oxidative stress Source: UniProtKB
Keywordsi
Molecular function | Chromatin regulator, DNA-binding |
Enzyme and pathway databases
PathwayCommonsi | O75367 |
Names & Taxonomyi
Protein namesi | Recommended name: Core histone macro-H2A.1Short name: Histone macroH2A1 Short name: mH2A1 Alternative name(s): Histone H2A.y Short name: H2A/y Medulloblastoma antigen MU-MB-50.205 |
Gene namesi | |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:4740, MACROH2A1 |
MIMi | 610054, gene |
neXtProti | NX_O75367 |
VEuPathDBi | HostDB:ENSG00000113648.16 |
Subcellular locationi
Nucleus
- Nucleus 3 Publications
Other locations
- Chromosome 3 Publications
Note: Enriched in inactive X chromosome chromatin and in senescence-associated heterochromatin.3 Publications
Extracellular region or secreted
- extracellular exosome Source: UniProtKB
Nucleus
- Barr body Source: UniProtKB
- nuclear chromosome Source: UniProtKB
- nucleolus Source: UniProtKB
- nucleoplasm Source: HPA
- nucleus Source: UniProtKB
Other locations
- chromatin Source: UniProtKB
- chromosome, telomeric region Source: BHF-UCL
- condensed chromosome Source: Ensembl
- nucleosome Source: UniProtKB
- pericentric heterochromatin Source: BHF-UCL
- sex chromatin Source: BHF-UCL
Keywords - Cellular componenti
Chromosome, Nucleosome core, NucleusPathology & Biotechi
Organism-specific databases
DisGeNETi | 9555 |
MalaCardsi | MACROH2A1 |
OpenTargetsi | ENSG00000113648 |
Orphaneti | 1275, Brachydactyly-elbow wrist dysplasia syndrome |
PharmGKBi | PA29117 |
Miscellaneous databases
Pharosi | O75367, Tbio |
Genetic variation databases
BioMutai | H2AFY |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000055318 | 1 – 372 | Core histone macro-H2A.1Add BLAST | 372 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 7 | N6-lactoyllysine; alternateBy similarity | 1 | |
Modified residuei | 9 | N6-lactoyllysine; alternateBy similarity | 1 | |
Modified residuei | 18 | N6-methyllysine1 Publication | 1 | |
Modified residuei | 116 | N6-acetyllysine; alternateBy similarity | 1 | |
Cross-linki | 116 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin); alternate1 Publication | ||
Cross-linki | 117 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)1 Publication | ||
Modified residuei | 123 | N6,N6-dimethyllysine; alternate1 Publication | 1 | |
Modified residuei | 123 | N6-acetyllysine; alternateBy similarity | 1 | |
Cross-linki | 123 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2); alternateCombined sources | ||
Modified residuei | 129 | PhosphothreonineCombined sources1 Publication | 1 | |
Cross-linki | 167 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources | ||
Modified residuei | 170 | PhosphoserineCombined sources | 1 | |
Modified residuei | 173 | PhosphoserineCombined sources | 1 | |
Modified residuei | 178 | PhosphothreonineCombined sources | 1 | |
Cross-linki | 189 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources | ||
Cross-linki | 323 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources |
Post-translational modificationi
Keywords - PTMi
Acetylation, Isopeptide bond, Methylation, Phosphoprotein, Ubl conjugationProteomic databases
CPTACi | CPTAC-383 CPTAC-384 |
EPDi | O75367 |
jPOSTi | O75367 |
MassIVEi | O75367 |
MaxQBi | O75367 |
PaxDbi | O75367 |
PeptideAtlasi | O75367 |
PRIDEi | O75367 |
ProteomicsDBi | 49934 [O75367-1] 49935 [O75367-2] 49936 [O75367-3] |
TopDownProteomicsi | O75367-1 [O75367-1] O75367-2 [O75367-2] |
2D gel databases
SWISS-2DPAGEi | O75367 |
PTM databases
GlyGeni | O75367, 1 site, 1 O-linked glycan (1 site) |
iPTMneti | O75367 |
PhosphoSitePlusi | O75367 |
SwissPalmi | O75367 |
Expressioni
Tissue specificityi
Gene expression databases
Bgeei | ENSG00000113648, Expressed in vagina and 247 other tissues |
ExpressionAtlasi | O75367, baseline and differential |
Genevisiblei | O75367, HS |
Organism-specific databases
HPAi | ENSG00000113648, Low tissue specificity |
Interactioni
Subunit structurei
The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers.
Interacts with HDAC1 and HDAC2 (By similarity).
Interacts with SPOP.
Part of a complex consisting of MACROH2A1, CUL3 and SPOP.
By similarity3 PublicationsBinary interactionsi
Hide detailsO75367
Isoform 1 [O75367-2]
With | #Exp. | IntAct |
---|---|---|
ATRX [P46100] | 2 | EBI-6249599,EBI-396461 |
TNKS [O95271] | 6 | EBI-6249599,EBI-1105254 |
Isoform 3 [O75367-3]
With | #Exp. | IntAct |
---|---|---|
ERBB2 [P04626] | 3 | EBI-6250866,EBI-641062 |
GO - Molecular functioni
- enzyme binding Source: BHF-UCL
- protein heterodimerization activity Source: InterPro
- protein kinase binding Source: UniProtKB
Protein-protein interaction databases
BioGRIDi | 114927, 235 interactors |
CORUMi | O75367 |
DIPi | DIP-44283N |
ELMi | O75367 |
IntActi | O75367, 68 interactors |
MINTi | O75367 |
STRINGi | 9606.ENSP00000423563 |
Miscellaneous databases
RNActi | O75367, protein |
Structurei
Secondary structure
3D structure databases
SMRi | O75367 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | O75367 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 2 – 117 | Histone H2AAdd BLAST | 116 | |
Domaini | 184 – 370 | MacroPROSITE-ProRule annotationAdd BLAST | 187 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 118 – 162 | Lys-richAdd BLAST | 45 |
Phylogenomic databases
eggNOGi | KOG1756, Eukaryota KOG2633, Eukaryota |
GeneTreei | ENSGT00940000159541 |
HOGENOMi | CLU_062828_0_0_1 |
InParanoidi | O75367 |
OMAi | AVIHPTN |
OrthoDBi | 1504122at2759 |
PhylomeDBi | O75367 |
TreeFami | TF332276 |
Family and domain databases
CDDi | cd00074, H2A, 1 hit cd02904, Macro_H2A-like, 1 hit |
Gene3Di | 1.10.20.10, 1 hit 3.40.220.10, 1 hit |
InterProi | View protein in InterPro IPR021171, Core_histone_macro-H2A IPR009072, Histone-fold IPR002119, Histone_H2A IPR007125, Histone_H2A/H2B/H3 IPR032454, Histone_H2A_C IPR002589, Macro_dom IPR043472, Macro_dom-like IPR035796, Macro_H2A |
Pfami | View protein in Pfam PF00125, Histone, 1 hit PF16211, Histone_H2A_C, 1 hit PF01661, Macro, 1 hit |
PIRSFi | PIRSF037942, Core_histone_macro-H2A, 1 hit |
PRINTSi | PR00620, HISTONEH2A |
SMARTi | View protein in SMART SM00506, A1pp, 1 hit SM00414, H2A, 1 hit |
SUPFAMi | SSF47113, SSF47113, 1 hit SSF52949, SSF52949, 1 hit |
PROSITEi | View protein in PROSITE PS51154, MACRO, 1 hit |
s (3+)i Sequence
Sequence statusi: Complete.
This entry describes 3 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 3 described isoforms and 2 potential isoforms that are computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MSSRGGKKKS TKTSRSAKAG VIFPVGRMLR YIKKGHPKYR IGVGAPVYMA
60 70 80 90 100
AVLEYLTAEI LELAGNAARD NKKGRVTPRH ILLAVANDEE LNQLLKGVTI
110 120 130 140 150
ASGGVLPNIH PELLAKKRGS KGKLEAIITP PPAKKAKSPS QKKPVSKKAG
160 170 180 190 200
GKKGARKSKK KQGEVSKAAS ADSTTEGTPA DGFTVLSTKS LFLGQKLNLI
210 220 230 240 250
HSEISNLAGF EVEAIINPTN ADIDLKDDLG NTLEKKGGKE FVEAVLELRK
260 270 280 290 300
KNGPLEVAGA AVSAGHGLPA KFVIHCNSPV WGADKCEELL EKTVKNCLAL
310 320 330 340 350
ADDKKLKSIA FPSIGSGRNG FPKQTAAQLI LKAISSYFVS TMSSSIKTVY
360 370
FVLFDSESIG IYVQEMAKLD AN
The sequence of this isoform differs from the canonical sequence as follows:
198-229: NLIHSEISNLAGFEVEAIINPTNADIDLKDDL → QVVQADIASIDSDAVVHPTNTDFYIGGEV
Computationally mapped potential isoform sequencesi
There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketD6RCF2 | D6RCF2_HUMAN | Histone H2A | MACROH2A1 | 165 | Annotation score: | ||
B4DJC3 | B4DJC3_HUMAN | Histone H2A | MACROH2A1 | 200 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 186 | L → F in BAB14565 (PubMed:14702039).Curated | 1 | |
Sequence conflicti | 210 | F → S in BAB14565 (PubMed:14702039).Curated | 1 | |
Sequence conflicti | 225 | L → P in AAC33433 (PubMed:9714746).Curated | 1 | |
Sequence conflicti | 291 | E → G in AAH95406 (PubMed:15489334).Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_038379 | 159 | Missing in isoform 3. 2 Publications | 1 | |
Alternative sequenceiVSP_002056 | 198 – 229 | NLIHS…LKDDL → QVVQADIASIDSDAVVHPTN TDFYIGGEV in isoform 1. 1 PublicationAdd BLAST | 32 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF041483 mRNA Translation: AAC33433.1 AF044286 mRNA Translation: AAC33434.1 AF054174 mRNA Translation: AAC39908.1 AK023409 mRNA Translation: BAB14565.1 AC026691 Genomic DNA No translation available. BC013331 mRNA Translation: AAH13331.1 BC095406 mRNA Translation: AAH95406.1 AY134746 mRNA Translation: AAN08620.1 |
CCDSi | CCDS4183.1 [O75367-3] CCDS4184.1 [O75367-2] CCDS4185.1 [O75367-1] |
RefSeqi | NP_001035248.1, NM_001040158.1 [O75367-3] NP_004884.1, NM_004893.2 [O75367-3] NP_613075.1, NM_138609.2 [O75367-2] NP_613258.2, NM_138610.2 [O75367-1] XP_011542031.1, XM_011543729.2 [O75367-1] XP_011542032.1, XM_011543730.2 [O75367-3] |
Genome annotation databases
Ensembli | ENST00000304332; ENSP00000302572; ENSG00000113648 [O75367-3] ENST00000312469; ENSP00000310169; ENSG00000113648 [O75367-2] ENST00000510038; ENSP00000424971; ENSG00000113648 [O75367-1] ENST00000511689; ENSP00000423563; ENSG00000113648 [O75367-1] |
GeneIDi | 9555 |
KEGGi | hsa:9555 |
UCSCi | uc003lam.2, human [O75367-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF041483 mRNA Translation: AAC33433.1 AF044286 mRNA Translation: AAC33434.1 AF054174 mRNA Translation: AAC39908.1 AK023409 mRNA Translation: BAB14565.1 AC026691 Genomic DNA No translation available. BC013331 mRNA Translation: AAH13331.1 BC095406 mRNA Translation: AAH95406.1 AY134746 mRNA Translation: AAN08620.1 |
CCDSi | CCDS4183.1 [O75367-3] CCDS4184.1 [O75367-2] CCDS4185.1 [O75367-1] |
RefSeqi | NP_001035248.1, NM_001040158.1 [O75367-3] NP_004884.1, NM_004893.2 [O75367-3] NP_613075.1, NM_138609.2 [O75367-2] NP_613258.2, NM_138610.2 [O75367-1] XP_011542031.1, XM_011543729.2 [O75367-1] XP_011542032.1, XM_011543730.2 [O75367-3] |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1U35 | X-ray | 3.00 | C/G | 1-120 | [»] | |
1ZR3 | X-ray | 1.66 | A/B/C/D | 162-372 | [»] | |
1ZR5 | X-ray | 2.92 | A/B | 161-372 | [»] | |
2F8N | X-ray | 2.90 | G | 1-120 | [»] | |
2FXK | X-ray | 2.54 | A/B | 162-372 | [»] | |
3HQH | X-ray | 2.30 | M | 167-181 | [»] | |
3HSV | X-ray | 1.43 | M | 172-186 | [»] | |
3IID | X-ray | 1.90 | A | 162-372 | [»] | |
3IIF | X-ray | 2.10 | A/B/C | 162-372 | [»] | |
3IVB | X-ray | 1.75 | M | 167-181 | [»] | |
5IIT | X-ray | 2.13 | A/B/C/D | 181-369 | [»] | |
5LNC | X-ray | 3.29 | A/B | 182-369 | [»] | |
SMRi | O75367 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 114927, 235 interactors |
CORUMi | O75367 |
DIPi | DIP-44283N |
ELMi | O75367 |
IntActi | O75367, 68 interactors |
MINTi | O75367 |
STRINGi | 9606.ENSP00000423563 |
PTM databases
GlyGeni | O75367, 1 site, 1 O-linked glycan (1 site) |
iPTMneti | O75367 |
PhosphoSitePlusi | O75367 |
SwissPalmi | O75367 |
Genetic variation databases
BioMutai | H2AFY |
2D gel databases
SWISS-2DPAGEi | O75367 |
Proteomic databases
CPTACi | CPTAC-383 CPTAC-384 |
EPDi | O75367 |
jPOSTi | O75367 |
MassIVEi | O75367 |
MaxQBi | O75367 |
PaxDbi | O75367 |
PeptideAtlasi | O75367 |
PRIDEi | O75367 |
ProteomicsDBi | 49934 [O75367-1] 49935 [O75367-2] 49936 [O75367-3] |
TopDownProteomicsi | O75367-1 [O75367-1] O75367-2 [O75367-2] |
Protocols and materials databases
Antibodypediai | 26391, 203 antibodies |
DNASUi | 9555 |
Genome annotation databases
Ensembli | ENST00000304332; ENSP00000302572; ENSG00000113648 [O75367-3] ENST00000312469; ENSP00000310169; ENSG00000113648 [O75367-2] ENST00000510038; ENSP00000424971; ENSG00000113648 [O75367-1] ENST00000511689; ENSP00000423563; ENSG00000113648 [O75367-1] |
GeneIDi | 9555 |
KEGGi | hsa:9555 |
UCSCi | uc003lam.2, human [O75367-1] |
Organism-specific databases
CTDi | 9555 |
DisGeNETi | 9555 |
GeneCardsi | MACROH2A1 |
HGNCi | HGNC:4740, MACROH2A1 |
HPAi | ENSG00000113648, Low tissue specificity |
MalaCardsi | MACROH2A1 |
MIMi | 610054, gene |
neXtProti | NX_O75367 |
OpenTargetsi | ENSG00000113648 |
Orphaneti | 1275, Brachydactyly-elbow wrist dysplasia syndrome |
PharmGKBi | PA29117 |
VEuPathDBi | HostDB:ENSG00000113648.16 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG1756, Eukaryota KOG2633, Eukaryota |
GeneTreei | ENSGT00940000159541 |
HOGENOMi | CLU_062828_0_0_1 |
InParanoidi | O75367 |
OMAi | AVIHPTN |
OrthoDBi | 1504122at2759 |
PhylomeDBi | O75367 |
TreeFami | TF332276 |
Enzyme and pathway databases
PathwayCommonsi | O75367 |
Miscellaneous databases
BioGRID-ORCSi | 9555, 3 hits in 999 CRISPR screens |
ChiTaRSi | H2AFY, human |
EvolutionaryTracei | O75367 |
GeneWikii | H2AFY |
GenomeRNAii | 9555 |
Pharosi | O75367, Tbio |
PROi | PR:O75367 |
RNActi | O75367, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000113648, Expressed in vagina and 247 other tissues |
ExpressionAtlasi | O75367, baseline and differential |
Genevisiblei | O75367, HS |
Family and domain databases
CDDi | cd00074, H2A, 1 hit cd02904, Macro_H2A-like, 1 hit |
Gene3Di | 1.10.20.10, 1 hit 3.40.220.10, 1 hit |
InterProi | View protein in InterPro IPR021171, Core_histone_macro-H2A IPR009072, Histone-fold IPR002119, Histone_H2A IPR007125, Histone_H2A/H2B/H3 IPR032454, Histone_H2A_C IPR002589, Macro_dom IPR043472, Macro_dom-like IPR035796, Macro_H2A |
Pfami | View protein in Pfam PF00125, Histone, 1 hit PF16211, Histone_H2A_C, 1 hit PF01661, Macro, 1 hit |
PIRSFi | PIRSF037942, Core_histone_macro-H2A, 1 hit |
PRINTSi | PR00620, HISTONEH2A |
SMARTi | View protein in SMART SM00506, A1pp, 1 hit SM00414, H2A, 1 hit |
SUPFAMi | SSF47113, SSF47113, 1 hit SSF52949, SSF52949, 1 hit |
PROSITEi | View protein in PROSITE PS51154, MACRO, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | H2AY_HUMAN | |
Accessioni | O75367Primary (citable) accession number: O75367 Secondary accession number(s): O75377 Q9UP96 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | January 24, 2001 |
Last sequence update: | January 23, 2007 | |
Last modified: | April 7, 2021 | |
This is version 210 of the entry and version 4 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Human chromosome 5
Human chromosome 5: entries, gene names and cross-references to MIM - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references