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Protein

Ectonucleoside triphosphate diphosphohydrolase 3

Gene

ENTPD3

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Has a threefold preference for the hydrolysis of ATP over ADP.

Catalytic activityi

A nucleoside 5'-triphosphate + 2 H2O = a nucleoside 5'-phosphate + 2 phosphate.

Cofactori

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei182Proton acceptorBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase
LigandATP-binding, Calcium, Magnesium, Nucleotide-binding

Enzyme and pathway databases

BRENDAi3.1.3.31 2681
ReactomeiR-HSA-8850843 Phosphate bond hydrolysis by NTPDase proteins

Names & Taxonomyi

Protein namesi
Recommended name:
Ectonucleoside triphosphate diphosphohydrolase 3 (EC:3.6.1.5)
Short name:
NTPDase 3
Alternative name(s):
CD39 antigen-like 3
Ecto-ATP diphosphohydrolase 3
Short name:
Ecto-ATPDase 3
Short name:
Ecto-ATPase 3
Ecto-apyrase 3
HB6
Gene namesi
Name:ENTPD3
Synonyms:CD39L3
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 3

Organism-specific databases

EuPathDBiHostDB:ENSG00000168032.8
HGNCiHGNC:3365 ENTPD3
MIMi603161 gene
neXtProtiNX_O75355

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 22CytoplasmicSequence analysisAdd BLAST22
Transmembranei23 – 43HelicalSequence analysisAdd BLAST21
Topological domaini44 – 485ExtracellularSequence analysisAdd BLAST442
Transmembranei486 – 506HelicalSequence analysisAdd BLAST21
Topological domaini507 – 529CytoplasmicSequence analysisAdd BLAST23

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi67R → G: Increase of activity. 1 Publication1
Mutagenesisi143R → A: Loss of activity. 1 Publication1
Mutagenesisi143R → K: Increase of activity. 1 Publication1
Mutagenesisi146R → N: No effect. 1 Publication1
Mutagenesisi146R → P: Increase of ATPase activity, decrease of ADPase activity. 1 Publication1
Mutagenesisi146R → T: Increase of activity. 1 Publication1
Mutagenesisi182E → D: Complete loss of activity. 1 Publication1
Mutagenesisi182E → Q: Complete loss of activity. 1 Publication1
Mutagenesisi187W → A: Complete loss of activity. 1 Publication1
Mutagenesisi191N → A: Loss of ATPase activity, increase of ADPase activity. 1 Publication1
Mutagenesisi219D → E: Increase of activity. 1 Publication1
Mutagenesisi224S → A: Complete loss of activity. 1 Publication1
Mutagenesisi226Q → A: Loss of activity. 1 Publication1
Mutagenesisi459W → A: Increase of activity, especially the ATP hydrolysis. 1 Publication1

Organism-specific databases

OpenTargetsiENSG00000168032
PharmGKBiPA27800

Chemistry databases

ChEMBLiCHEMBL5897

Polymorphism and mutation databases

BioMutaiENTPD3

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002099101 – 529Ectonucleoside triphosphate diphosphohydrolase 3Add BLAST529

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi81N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi92 ↔ 1161 Publication
Glycosylationi149N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi238N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi261 ↔ 3081 Publication
Disulfide bondi289 ↔ 3341 Publication
Disulfide bondi347 ↔ 3531 Publication
Glycosylationi381N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi392N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi399 ↔ 4221 Publication
Glycosylationi402N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi454N-linked (GlcNAc...) asparagineSequence analysis1

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiO75355
PeptideAtlasiO75355
PRIDEiO75355
ProteomicsDBi49922

PTM databases

iPTMnetiO75355
PhosphoSitePlusiO75355

Expressioni

Tissue specificityi

Expressed in adult brain, pancreas, spleen and prostate. Moderate or low expression is seen in most tissues. Not expressed in liver and peripheral blood leukocytes.

Gene expression databases

BgeeiENSG00000168032 Expressed in 175 organ(s), highest expression level in caudate nucleus
CleanExiHS_ENTPD3
ExpressionAtlasiO75355 baseline and differential
GenevisibleiO75355 HS

Organism-specific databases

HPAiHPA071802

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
SYNE4Q8N2053EBI-10187968,EBI-7131783

Protein-protein interaction databases

BioGridi107394, 2 interactors
IntActiO75355, 13 interactors
STRINGi9606.ENSP00000301825

Chemistry databases

BindingDBiO75355

Structurei

3D structure databases

ProteinModelPortaliO75355
SMRiO75355
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the GDA1/CD39 NTPase family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG1386 Eukaryota
COG5371 LUCA
GeneTreeiENSGT00550000074435
HOGENOMiHOG000059572
HOVERGENiHBG018982
InParanoidiO75355
KOiK01510
OMAiAFEDCMQ
OrthoDBiEOG091G05FZ
PhylomeDBiO75355
TreeFamiTF332859

Family and domain databases

InterProiView protein in InterPro
IPR000407 GDA1_CD39_NTPase
PANTHERiPTHR11782 PTHR11782, 1 hit
PfamiView protein in Pfam
PF01150 GDA1_CD39, 1 hit
PROSITEiView protein in PROSITE
PS01238 GDA1_CD39_NTPASE, 1 hit

Sequences (2+)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 2 described isoforms and 1 potential isoform that is computationally mapped.Show allAlign All

Isoform 1 (identifier: O75355-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MFTVLTRQPC EQAGLKALYR TPTIIALVVL LVSIVVLVSI TVIQIHKQEV
60 70 80 90 100
LPPGLKYGIV LDAGSSRTTV YVYQWPAEKE NNTGVVSQTF KCSVKGSGIS
110 120 130 140 150
SYGNNPQDVP RAFEECMQKV KGQVPSHLHG STPIHLGATA GMRLLRLQNE
160 170 180 190 200
TAANEVLESI QSYFKSQPFD FRGAQIISGQ EEGVYGWITA NYLMGNFLEK
210 220 230 240 250
NLWHMWVHPH GVETTGALDL GGASTQISFV AGEKMDLNTS DIMQVSLYGY
260 270 280 290 300
VYTLYTHSFQ CYGRNEAEKK FLAMLLQNSP TKNHLTNPCY PRDYSISFTM
310 320 330 340 350
GHVFDSLCTV DQRPESYNPN DVITFEGTGD PSLCKEKVAS IFDFKACHDQ
360 370 380 390 400
ETCSFDGVYQ PKIKGPFVAF AGFYYTASAL NLSGSFSLDT FNSSTWNFCS
410 420 430 440 450
QNWSQLPLLL PKFDEVYARS YCFSANYIYH LFVNGYKFTE ETWPQIHFEK
460 470 480 490 500
EVGNSSIAWS LGYMLSLTNQ IPAESPLIRL PIEPPVFVGT LAFFTAAALL
510 520
CLAFLAYLCS ATRRKRHSEH AFDHAVDSD
Length:529
Mass (Da):59,105
Last modified:September 19, 2006 - v2
Checksum:i5043CF0202978B88
GO
Isoform 2 (identifier: O75355-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     452-529: VGNSSIAWSL...HAFDHAVDSD → E

Note: No experimental confirmation available.
Show »
Length:452
Mass (Da):50,762
Checksum:i28E1A42977EA63DC
GO

Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
C9J0J3C9J0J3_HUMAN
Ectonucleoside triphosphate diphosp...
ENTPD3
130Annotation score:

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_07081324I → V1 PublicationCorresponds to variant dbSNP:rs17852714Ensembl.1
Natural variantiVAR_061384264R → Q. Corresponds to variant dbSNP:rs34266806Ensembl.1
Natural variantiVAR_027541440E → D. Corresponds to variant dbSNP:rs4470483Ensembl.1
Natural variantiVAR_027542496A → V1 PublicationCorresponds to variant dbSNP:rs1047855Ensembl.1
Natural variantiVAR_027543505L → F. Corresponds to variant dbSNP:rs3733167Ensembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_054237452 – 529VGNSS…AVDSD → E in isoform 2. 1 PublicationAdd BLAST78

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF039917 mRNA Translation: AAC39884.1
AF034840 mRNA Translation: AAC09236.2
AK313322 mRNA Translation: BAG36127.1
AC104186 Genomic DNA No translation available.
CH471055 Genomic DNA Translation: EAW64600.1
CH471055 Genomic DNA Translation: EAW64601.1
BC029869 mRNA Translation: AAH29869.1
CCDSiCCDS2691.1 [O75355-1]
CCDS74919.1 [O75355-2]
RefSeqiNP_001239.2, NM_001248.3 [O75355-1]
NP_001278889.1, NM_001291960.1 [O75355-1]
NP_001278890.1, NM_001291961.1 [O75355-2]
UniGeneiHs.441145

Genome annotation databases

EnsembliENST00000301825; ENSP00000301825; ENSG00000168032 [O75355-1]
ENST00000445129; ENSP00000404671; ENSG00000168032 [O75355-2]
ENST00000456402; ENSP00000401565; ENSG00000168032 [O75355-1]
GeneIDi956
KEGGihsa:956
UCSCiuc003ckd.5 human [O75355-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF039917 mRNA Translation: AAC39884.1
AF034840 mRNA Translation: AAC09236.2
AK313322 mRNA Translation: BAG36127.1
AC104186 Genomic DNA No translation available.
CH471055 Genomic DNA Translation: EAW64600.1
CH471055 Genomic DNA Translation: EAW64601.1
BC029869 mRNA Translation: AAH29869.1
CCDSiCCDS2691.1 [O75355-1]
CCDS74919.1 [O75355-2]
RefSeqiNP_001239.2, NM_001248.3 [O75355-1]
NP_001278889.1, NM_001291960.1 [O75355-1]
NP_001278890.1, NM_001291961.1 [O75355-2]
UniGeneiHs.441145

3D structure databases

ProteinModelPortaliO75355
SMRiO75355
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi107394, 2 interactors
IntActiO75355, 13 interactors
STRINGi9606.ENSP00000301825

Chemistry databases

BindingDBiO75355
ChEMBLiCHEMBL5897

PTM databases

iPTMnetiO75355
PhosphoSitePlusiO75355

Polymorphism and mutation databases

BioMutaiENTPD3

Proteomic databases

PaxDbiO75355
PeptideAtlasiO75355
PRIDEiO75355
ProteomicsDBi49922

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000301825; ENSP00000301825; ENSG00000168032 [O75355-1]
ENST00000445129; ENSP00000404671; ENSG00000168032 [O75355-2]
ENST00000456402; ENSP00000401565; ENSG00000168032 [O75355-1]
GeneIDi956
KEGGihsa:956
UCSCiuc003ckd.5 human [O75355-1]

Organism-specific databases

CTDi956
EuPathDBiHostDB:ENSG00000168032.8
GeneCardsiENTPD3
HGNCiHGNC:3365 ENTPD3
HPAiHPA071802
MIMi603161 gene
neXtProtiNX_O75355
OpenTargetsiENSG00000168032
PharmGKBiPA27800
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG1386 Eukaryota
COG5371 LUCA
GeneTreeiENSGT00550000074435
HOGENOMiHOG000059572
HOVERGENiHBG018982
InParanoidiO75355
KOiK01510
OMAiAFEDCMQ
OrthoDBiEOG091G05FZ
PhylomeDBiO75355
TreeFamiTF332859

Enzyme and pathway databases

BRENDAi3.1.3.31 2681
ReactomeiR-HSA-8850843 Phosphate bond hydrolysis by NTPDase proteins

Miscellaneous databases

GeneWikiiENTPD3
GenomeRNAii956
PROiPR:O75355
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000168032 Expressed in 175 organ(s), highest expression level in caudate nucleus
CleanExiHS_ENTPD3
ExpressionAtlasiO75355 baseline and differential
GenevisibleiO75355 HS

Family and domain databases

InterProiView protein in InterPro
IPR000407 GDA1_CD39_NTPase
PANTHERiPTHR11782 PTHR11782, 1 hit
PfamiView protein in Pfam
PF01150 GDA1_CD39, 1 hit
PROSITEiView protein in PROSITE
PS01238 GDA1_CD39_NTPASE, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiENTP3_HUMAN
AccessioniPrimary (citable) accession number: O75355
Secondary accession number(s): B2R8D0
, G5E9N0, O60495, Q8N6K2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 29, 2001
Last sequence update: September 19, 2006
Last modified: November 7, 2018
This is version 151 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Human chromosome 3
    Human chromosome 3: entries, gene names and cross-references to MIM
  3. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  4. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  5. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
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