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Protein

CCR4-NOT transcription complex subunit 3

Gene

CNOT3

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Component of the CCR4-NOT complex which is one of the major cellular mRNA deadenylases and is linked to various cellular processes including bulk mRNA degradation, miRNA-mediated repression, translational repression during translational initiation and general transcription regulation. Additional complex functions may be a consequence of its influence on mRNA expression. May be involved in metabolic regulation; may be involved in recruitment of the CCR4-NOT complex to deadenylation target mRNAs involved in energy metabolism. Involved in mitotic progression and regulation of the spindle assembly checkpoint by regulating the stability of MAD1L1 mRNA. Can repress transcription and may link the CCR4-NOT complex to transcriptional regulation; the repressive function may involve histone deacetylases. Involved in the maintenance of emryonic stem (ES) cell identity.3 Publications

GO - Biological processi

Keywordsi

Molecular functionDevelopmental protein, Repressor
Biological processRNA-mediated gene silencing, Transcription, Transcription regulation, Translation regulation

Enzyme and pathway databases

ReactomeiR-HSA-429947 Deadenylation of mRNA
R-HSA-6804115 TP53 regulates transcription of additional cell cycle genes whose exact role in the p53 pathway remain uncertain
SIGNORiO75175

Names & Taxonomyi

Protein namesi
Recommended name:
CCR4-NOT transcription complex subunit 3
Alternative name(s):
CCR4-associated factor 3
Leukocyte receptor cluster member 2
Gene namesi
Name:CNOT3
Synonyms:KIAA0691, LENG2, NOT3
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 19

Organism-specific databases

EuPathDBiHostDB:ENSG00000088038.17
HGNCiHGNC:7879 CNOT3
MIMi604910 gene
neXtProtiNX_O75175

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Organism-specific databases

DisGeNETi4849
MalaCardsiCNOT3
OpenTargetsiENSG00000088038
Orphaneti99861 Precursor T-cell acute lymphoblastic leukemia
PharmGKBiPA26674

Polymorphism and mutation databases

BioMutaiCNOT3

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001983331 – 753CCR4-NOT transcription complex subunit 3Add BLAST753

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei292PhosphothreonineCombined sources1
Modified residuei299PhosphoserineCombined sources1
Modified residuei542PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiO75175
MaxQBiO75175
PaxDbiO75175
PeptideAtlasiO75175
PRIDEiO75175
ProteomicsDBi49842

PTM databases

iPTMnetiO75175
PhosphoSitePlusiO75175

Expressioni

Tissue specificityi

Ubiquitous. Highly expressed in brain, heart, thymus, spleen, kidney, liver, small intestine, lung and peripheral blood leukocytes.1 Publication

Developmental stagei

Expressed in embryonic stem (ES) cells.1 Publication

Gene expression databases

BgeeiENSG00000088038
CleanExiHS_CNOT3
ExpressionAtlasiO75175 baseline and differential
GenevisibleiO75175 HS

Organism-specific databases

HPAiHPA006408

Interactioni

Subunit structurei

Component of the CCR4-NOT complex; distinct complexes seem to exist that differ in the participation of probably mutually exclusive catalytic subunits. In the complex interacts directly with CNOT2. Interacts with TIP120B and NANOS2.4 Publications

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi110911, 55 interactors
CORUMiO75175
DIPiDIP-40297N
IntActiO75175, 26 interactors
MINTiO75175
STRINGi9606.ENSP00000221232

Structurei

Secondary structure

1753
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi609 – 623Combined sources15
Helixi628 – 631Combined sources4
Beta strandi636 – 638Combined sources3
Turni655 – 658Combined sources4
Helixi660 – 665Combined sources6
Helixi668 – 677Combined sources10
Turni678 – 680Combined sources3
Helixi682 – 693Combined sources12
Beta strandi697 – 699Combined sources3
Turni700 – 703Combined sources4
Beta strandi704 – 710Combined sources7
Beta strandi713 – 715Combined sources3
Beta strandi717 – 728Combined sources12
Turni729 – 732Combined sources4
Beta strandi733 – 743Combined sources11
Helixi744 – 746Combined sources3

3D structure databases

ProteinModelPortaliO75175
SMRiO75175
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni661 – 753Repressor domainAdd BLAST93

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi314 – 395Pro-richAdd BLAST82
Compositional biasi397 – 425Gly/Ser-richAdd BLAST29
Compositional biasi457 – 655Pro-richAdd BLAST199

Sequence similaritiesi

Belongs to the CNOT2/3/5 family.Curated

Phylogenomic databases

eggNOGiKOG2150 Eukaryota
COG5665 LUCA
GeneTreeiENSGT00390000014743
HOGENOMiHOG000184850
HOVERGENiHBG051042
InParanoidiO75175
KOiK12580
OrthoDBiEOG091G040M
PhylomeDBiO75175
TreeFamiTF321963

Family and domain databases

Gene3Di2.30.30.1020, 1 hit
InterProiView protein in InterPro
IPR038635 CCR4-NOT_su2/3/5_N_sf
IPR012270 CCR4-NOT_su3/5
IPR007282 NOT
IPR007207 Not_N
PfamiView protein in Pfam
PF04153 NOT2_3_5, 1 hit
PF04065 Not3, 1 hit
PIRSFiPIRSF005290 NOT_su_3_5, 1 hit

Sequencei

Sequence statusi: Complete.

O75175-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MADKRKLQGE IDRCLKKVSE GVEQFEDIWQ KLHNAANANQ KEKYEADLKK
60 70 80 90 100
EIKKLQRLRD QIKTWVASNE IKDKRQLIDN RKLIETQMER FKVVERETKT
110 120 130 140 150
KAYSKEGLGL AQKVDPAQKE KEEVGQWLTN TIDTLNMQVD QFESEVESLS
160 170 180 190 200
VQTRKKKGDK DKQDRIEGLK RHIEKHRYHV RMLETILRML DNDSILVDAI
210 220 230 240 250
RKIKDDVEYY VDSSQDPDFE ENEFLYDDLD LEDIPQALVA TSPPSHSHME
260 270 280 290 300
DEIFNQSSST PTSTTSSSPI PPSPANCTTE NSEDDKKRGR STDSEVSQSP
310 320 330 340 350
AKNGSKPVHS NQHPQSPAVP PTYPSGPPPA ASALSTTPGN NGVPAPAAPP
360 370 380 390 400
SALGPKASPA PSHNSGTPAP YAQAVAPPAP SGPSTTQPRP PSVQPSGGGG
410 420 430 440 450
GGSGGGGSSS SSNSSAGGGA GKQNGATSYS SVVADSPAEV ALSSSGGNNA
460 470 480 490 500
SSQALGPPSG PHNPPPSTSK EPSAAAPTGA GGVAPGSGNN SGGPSLLVPL
510 520 530 540 550
PVNPPSSPTP SFSDAKAAGA LLNGPPQFST APEIKAPEPL SSLKSMAERA
560 570 580 590 600
AISSGIEDPV PTLHLTERDI ILSSTSAPPA SAQPPLQLSE VNIPLSLGVC
610 620 630 640 650
PLGPVPLTKE QLYQQAMEEA AWHHMPHPSD SERIRQYLPR NPCPTPPYHH
660 670 680 690 700
QMPPPHSDTV EFYQRLSTET LFFIFYYLEG TKAQYLAAKA LKKQSWRFHT
710 720 730 740 750
KYMMWFQRHE EPKTITDEFE QGTYIYFDYE KWGQRKKEGF TFEYRYLEDR

DLQ
Length:753
Mass (Da):81,872
Last modified:November 1, 1998 - v1
Checksum:iC9D4F8B9FEF6CD90
GO

Sequence cautioni

The sequence AAF29828 differs from that shown. Seems to have an artifactual loop-out at the 3'-end deleting 2 full exons and part of 2 others.Curated
The sequence BAA31666 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated
The sequence CAB63766 differs from that shown. Reason: Erroneous translation. Wrong choice of frame.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB014591 mRNA Translation: BAA31666.2 Different initiation.
BC016474 mRNA Translation: AAH16474.1
AF180474 mRNA Translation: AAF29828.1 Sequence problems.
AL133647 mRNA Translation: CAB63766.1 Sequence problems.
CCDSiCCDS12880.1
PIRiT43456
RefSeqiNP_055331.1, NM_014516.3
UniGeneiHs.343571

Genome annotation databases

EnsembliENST00000221232; ENSP00000221232; ENSG00000088038
ENST00000358389; ENSP00000351159; ENSG00000088038
ENST00000610883; ENSP00000480258; ENSG00000274941
ENST00000611252; ENSP00000483968; ENSG00000275979
ENST00000612924; ENSP00000480585; ENSG00000274176
ENST00000613528; ENSP00000483604; ENSG00000277615
ENST00000613752; ENSP00000481681; ENSG00000273943
ENST00000614649; ENSP00000484794; ENSG00000274176
ENST00000615030; ENSP00000480697; ENSG00000274616
ENST00000616359; ENSP00000484040; ENSG00000274616
ENST00000616910; ENSP00000480876; ENSG00000277615
ENST00000617982; ENSP00000479987; ENSG00000277600
ENST00000618405; ENSP00000481924; ENSG00000277114
ENST00000619567; ENSP00000483882; ENSG00000275979
ENST00000619854; ENSP00000483431; ENSG00000273943
ENST00000620060; ENSP00000483036; ENSG00000277114
ENST00000620419; ENSP00000478956; ENSG00000276082
ENST00000620573; ENSP00000480988; ENSG00000276082
ENST00000620970; ENSP00000483692; ENSG00000277600
ENST00000622131; ENSP00000478280; ENSG00000274941
GeneIDi4849
KEGGihsa:4849
UCSCiuc002qdj.3 human

Similar proteinsi

Entry informationi

Entry nameiCNOT3_HUMAN
AccessioniPrimary (citable) accession number: O75175
Secondary accession number(s): Q9NZN7, Q9UF76
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 13, 2004
Last sequence update: November 1, 1998
Last modified: June 20, 2018
This is version 156 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 19
    Human chromosome 19: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

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