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UniProtKB - O75150 (BRE1B_HUMAN)

Protein

E3 ubiquitin-protein ligase BRE1B

Gene

RNF40

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Component of the RNF20/40 E3 ubiquitin-protein ligase complex that mediates monoubiquitination of 'Lys-120' of histone H2B (H2BK120ub1). H2BK120ub1 gives a specific tag for epigenetic transcriptional activation and is also prerequisite for histone H3 'Lys-4' and 'Lys-79' methylation (H3K4me and H3K79me, respectively). It thereby plays a central role in histone code and gene regulation. The RNF20/40 complex forms a H2B ubiquitin ligase complex in cooperation with the E2 enzyme UBE2A or UBE2B; reports about the cooperation with UBE2E1/UBCH are contradictory. Required for transcriptional activation of Hox genes.2 Publications

Catalytic activityi

S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N6-ubiquitinyl-[acceptor protein]-L-lysine.2 Publications

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri948 – 987RING-typePROSITE-ProRule annotationAdd BLAST40

GO - Molecular functioni

View the complete GO annotation on QuickGO ...

GO - Biological processi

View the complete GO annotation on QuickGO ...

Keywordsi

Molecular functionChromatin regulator, Transferase
Biological processUbl conjugation pathway
LigandMetal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-HSA-8866654 E3 ubiquitin ligases ubiquitinate target proteins
SIGNORiO75150
UniPathwayi
UPA00143

Names & Taxonomyi

Protein namesi
Recommended name:
E3 ubiquitin-protein ligase BRE1B (EC:2.3.2.272 Publications)
Short name:
BRE1-B
Alternative name(s):
95 kDa retinoblastoma-associated protein
Short name:
RBP95
RING finger protein 40
RING-type E3 ubiquitin transferase BRE1BCurated
Gene namesi
Name:RNF40
Synonyms:BRE1B, KIAA0661
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo
Proteomesi
  • UP000005640 Componenti: Chromosome 16

Organism-specific databases

EuPathDBiHostDB:ENSG00000103549.21
HGNCiHGNC:16867 RNF40
MIMi607700 gene
neXtProtiNX_O75150

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi109L → S: Abolishes interaction with RB1. 1 Publication1
Mutagenesisi111C → M: Abolishes interaction with RB1. 1 Publication1
Mutagenesisi113E → Q: Abolishes interaction with RB1. 1 Publication1

Organism-specific databases

DisGeNETi9810
OpenTargetsiENSG00000103549
PharmGKBiPA34440

Polymorphism and mutation databases

BioMutaiRNF40

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000558391 – 1001E3 ubiquitin-protein ligase BRE1BAdd BLAST1001

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei20N6-acetyllysineBy similarity1
Modified residuei42PhosphoserineBy similarity1
Modified residuei355N6-acetyllysineBy similarity1
Modified residuei517N6-acetyllysineBy similarity1
Modified residuei528PhosphoserineBy similarity1
Cross-linki578Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Cross-linki579Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Modified residuei585PhosphoserineBy similarity1

Keywords - PTMi

Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiO75150
MaxQBiO75150
PaxDbiO75150
PeptideAtlasiO75150
PRIDEiO75150
ProteomicsDBi49814
49815 [O75150-3]
49816 [O75150-4]

PTM databases

iPTMnetiO75150
PhosphoSitePlusiO75150

Expressioni

Tissue specificityi

Ubiquitously expressed. Expressed at higher level in testis, heart and pancreas, while it is only weakly expressed in lung, skeletal muscle and small intestine.1 Publication

Gene expression databases

BgeeiENSG00000103549 Expressed in 179 organ(s), highest expression level in testis
CleanExiHS_RNF40
ExpressionAtlasiO75150 baseline and differential
GenevisibleiO75150 HS

Organism-specific databases

HPAiHPA041330
HPA054227

Interactioni

Subunit structurei

Component of the RNF20/40 complex (also known as BRE1 complex) probably composed of 2 copies of RNF20/BRE1A and 2 copies of RNF40/BRE1B. Interacts with UBE2E1/UBCH6. Interacts with RB1 and WAC.4 Publications

Binary interactionsi

GO - Molecular functioni

View the complete GO annotation on QuickGO ...

Protein-protein interaction databases

BioGridi115149, 93 interactors
CORUMiO75150
IntActiO75150, 36 interactors
MINTiO75150
STRINGi9606.ENSP00000325677

Structurei

3D structure databases

ProteinModelPortaliO75150
SMRiO75150
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili44 – 91Sequence analysisAdd BLAST48
Coiled coili189 – 377Sequence analysisAdd BLAST189
Coiled coili437 – 525Sequence analysisAdd BLAST89
Coiled coili627 – 946Sequence analysisAdd BLAST320

Sequence similaritiesi

Belongs to the BRE1 family.Curated

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri948 – 987RING-typePROSITE-ProRule annotationAdd BLAST40

Keywords - Domaini

Coiled coil, Zinc-finger

Phylogenomic databases

eggNOGiKOG0978 Eukaryota
ENOG410Y5C6 LUCA
GeneTreeiENSGT00390000002866
HOVERGENiHBG080312
InParanoidiO75150
KOiK10696
OMAiNDQKEMK
OrthoDBiEOG091G01SA
PhylomeDBiO75150
TreeFamiTF323183

Family and domain databases

Gene3Di3.30.40.10, 1 hit
InterProiView protein in InterPro
IPR013956 E3_ubiquit_lig_Bre1
IPR001841 Znf_RING
IPR013083 Znf_RING/FYVE/PHD
IPR017907 Znf_RING_CS
PANTHERiPTHR23163 PTHR23163, 1 hit
SMARTiView protein in SMART
SM00184 RING, 1 hit
PROSITEiView protein in PROSITE
PS00518 ZF_RING_1, 1 hit
PS50089 ZF_RING_2, 1 hit

Sequences (3+)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 3 described isoforms and 7 potential isoforms that are computationally mapped.Show allAlign All

Isoform 1 (identifier: O75150-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MSGPGNKRAA GDGGSGPPEK KLSREEKTTT TLIEPIRLGG ISSTEEMDLK 
        60         70         80         90        100
VLQFKNKKLA ERLEQRQACE DELRERIEKL EKRQATDDAT LLIVNRYWAQ 
       110        120        130        140        150
LDETVEALLR CHESQGELSS APEAPGTQEG PTCDGTPLPE PGTSELRDPL 
       160        170        180        190        200
LMQLRPPLSE PALAFVVALG ASSSEEVELE LQGRMEFSKA AVSRVVEASD 
       210        220        230        240        250
RLQRRVEELC QRVYSRGDSE PLSEAAQAHT RELGRENRRL QDLATQLQEK 
       260        270        280        290        300
HHRISLEYSE LQDKVTSAET KVLEMETTVE DLQWDIEKLR KREQKLNKHL 
       310        320        330        340        350
AEALEQLNSG YYVSGSSSGF QGGQITLSMQ KFEMLNAELE ENQELANSRM 
       360        370        380        390        400
AELEKLQAEL QGAVRTNERL KVALRSLPEE VVRETGEYRM LQAQFSLLYN 
       410        420        430        440        450
ESLQVKTQLD EARGLLLATK NSHLRHIEHM ESDELGLQKK LRTEVIQLED 
       460        470        480        490        500
TLAQVRKEYE MLRIEFEQNL AANEQAGPIN REMRHLISSL QNHNHQLKGD 
       510        520        530        540        550
AQRYKRKLRE VQAEIGKLRA QASGSAHSTP NLGHPEDSGV SAPAPGKEEG 
       560        570        580        590        600
GPGPVSTPDN RKEMAPVPGT TTTTTSVKKE ELVPSEEDFQ GITPGAQGPS 
       610        620        630        640        650
SRGREPEARP KRELREREGP SLGPPPVASA LSRADREKAK VEETKRKESE 
       660        670        680        690        700
LLKGLRAELK KAQESQKEMK LLLDMYKSAP KEQRDKVQLM AAERKAKAEV 
       710        720        730        740        750
DELRSRIREL EERDRRESKK IADEDALRRI RQAEEQIEHL QRKLGATKQE 
       760        770        780        790        800
EEALLSEMDV TGQAFEDMQE QNGRLLQQLR EKDDANFKLM SERIKANQIH 
       810        820        830        840        850
KLLREEKDEL GEQVLGLKSQ VDAQLLTVQK LEEKERALQG SLGGVEKELT 
       860        870        880        890        900
LRSQALELNK RKAVEAAQLA EDLKVQLEHV QTRLREIQPC LAESRAAREK 
       910        920        930        940        950
ESFNLKRAQE DISRLRRKLE KQRKVEVYAD ADEILQEEIK EYKARLTCPC 
       960        970        980        990       1000
CNTRKKDAVL TKCFHVFCFE CVRGRYEARQ RKCPKCNAAF GAHDFHRIYI 

S
Length:1,001
Mass (Da):113,678
Last modified:September 12, 2018 - v5
Checksum:i408A6E5A9CC25E41
GO
Isoform 3 (identifier: O75150-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     822-838: DAQLLTVQKLEEKERAL → WPPPACRLELERWGSWP
     839-1001: Missing.

Note: No experimental confirmation available.
Show »
Length:838
Mass (Da):94,824
Checksum:i049543CA9F7A651C
GO
Isoform 4 (identifier: O75150-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     332-431: Missing.

Note: No experimental confirmation available.
Show »
Length:901
Mass (Da):102,074
Checksum:i8600705E554E12B8
GO

Computationally mapped potential isoform sequencesi

There are 7 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
H3BP71H3BP71_HUMAN
E3 ubiquitin protein ligase
RNF40
961Annotation score:
H3BUL6H3BUL6_HUMAN
E3 ubiquitin protein ligase
RNF40
129Annotation score:
I3L332I3L332_HUMAN
E3 ubiquitin protein ligase
RNF40
144Annotation score:
H3BS50H3BS50_HUMAN
E3 ubiquitin protein ligase
RNF40
174Annotation score:
H3BQ99H3BQ99_HUMAN
E3 ubiquitin protein ligase
RNF40
111Annotation score:
A0A087WTK2A0A087WTK2_HUMAN
E3 ubiquitin protein ligase
RNF40
177Annotation score:
R4GN66R4GN66_HUMAN
E3 ubiquitin-protein ligase BRE1B
RNF40
14Annotation score:

Sequence cautioni

The sequence BAA31636 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated
The sequence CAE45869 differs from that shown. Aberrant splicing.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti56N → D in BAB55092 (PubMed:14702039).Curated1
Sequence conflicti96R → L in AAG13723 (PubMed:10944455).Curated1
Sequence conflicti108L → P in BAB55092 (PubMed:14702039).Curated1
Sequence conflicti172S → G in BAB55092 (PubMed:14702039).Curated1
Sequence conflicti387E → K in CAH10518 (PubMed:17974005).Curated1
Sequence conflicti562K → E in CAE45869 (PubMed:17974005).Curated1
Sequence conflicti600S → F in CAE45869 (PubMed:17974005).Curated1
Sequence conflicti714D → N in BAB55092 (PubMed:14702039).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_055021463R → H. Corresponds to variant dbSNP:rs11556801Ensembl.1
Natural variantiVAR_055022615R → Q. Corresponds to variant dbSNP:rs7195142Ensembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_016681332 – 431Missing in isoform 4. 1 PublicationAdd BLAST100
Alternative sequenceiVSP_016682822 – 838DAQLL…KERAL → WPPPACRLELERWGSWP in isoform 3. 1 PublicationAdd BLAST17
Alternative sequenceiVSP_016683839 – 1001Missing in isoform 3. 1 PublicationAdd BLAST163

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF122819 mRNA Translation: AAG13723.1
AB014561 mRNA Translation: BAA31636.2 Different initiation.
AK027406 mRNA Translation: BAB55092.1
BX640763 mRNA Translation: CAE45869.1 Sequence problems.
CR627431 mRNA Translation: CAH10518.1
AC106886 Genomic DNA No translation available.
CH471192 Genomic DNA Translation: EAW52197.1
CH471192 Genomic DNA Translation: EAW52200.1
BC004527 mRNA Translation: AAH04527.2
BC006133 mRNA Translation: AAH06133.1
BC011769 mRNA Translation: AAH11769.1
BC018647 mRNA Translation: AAH18647.1
BC030802 mRNA Translation: AAH30802.2
CCDSiCCDS10691.1 [O75150-1]
CCDS55994.1 [O75150-4]
PIRiJC7363
RefSeqiNP_001193962.1, NM_001207033.1
NP_001193963.1, NM_001207034.1 [O75150-4]
NP_001273501.1, NM_001286572.2 [O75150-1]
NP_055586.1, NM_014771.3 [O75150-1]
UniGeneiHs.65238

Genome annotation databases

EnsembliENST00000324685; ENSP00000325677; ENSG00000103549 [O75150-1]
ENST00000357890; ENSP00000350563; ENSG00000103549 [O75150-4]
GeneIDi9810
KEGGihsa:9810
UCSCiuc002dzq.4 human [O75150-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF122819 mRNA Translation: AAG13723.1
AB014561 mRNA Translation: BAA31636.2 Different initiation.
AK027406 mRNA Translation: BAB55092.1
BX640763 mRNA Translation: CAE45869.1 Sequence problems.
CR627431 mRNA Translation: CAH10518.1
AC106886 Genomic DNA No translation available.
CH471192 Genomic DNA Translation: EAW52197.1
CH471192 Genomic DNA Translation: EAW52200.1
BC004527 mRNA Translation: AAH04527.2
BC006133 mRNA Translation: AAH06133.1
BC011769 mRNA Translation: AAH11769.1
BC018647 mRNA Translation: AAH18647.1
BC030802 mRNA Translation: AAH30802.2
CCDSiCCDS10691.1 [O75150-1]
CCDS55994.1 [O75150-4]
PIRiJC7363
RefSeqiNP_001193962.1, NM_001207033.1
NP_001193963.1, NM_001207034.1 [O75150-4]
NP_001273501.1, NM_001286572.2 [O75150-1]
NP_055586.1, NM_014771.3 [O75150-1]
UniGeneiHs.65238

3D structure databases

ProteinModelPortaliO75150
SMRiO75150
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi115149, 93 interactors
CORUMiO75150
IntActiO75150, 36 interactors
MINTiO75150
STRINGi9606.ENSP00000325677

PTM databases

iPTMnetiO75150
PhosphoSitePlusiO75150

Polymorphism and mutation databases

BioMutaiRNF40

Proteomic databases

EPDiO75150
MaxQBiO75150
PaxDbiO75150
PeptideAtlasiO75150
PRIDEiO75150
ProteomicsDBi49814
49815 [O75150-3]
49816 [O75150-4]

Protocols and materials databases

DNASUi9810
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000324685; ENSP00000325677; ENSG00000103549 [O75150-1]
ENST00000357890; ENSP00000350563; ENSG00000103549 [O75150-4]
GeneIDi9810
KEGGihsa:9810
UCSCiuc002dzq.4 human [O75150-1]

Organism-specific databases

CTDi9810
DisGeNETi9810
EuPathDBiHostDB:ENSG00000103549.21
GeneCardsiRNF40
HGNCiHGNC:16867 RNF40
HPAiHPA041330
HPA054227
MIMi607700 gene
neXtProtiNX_O75150
OpenTargetsiENSG00000103549
PharmGKBiPA34440
HUGEiSearch...
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG0978 Eukaryota
ENOG410Y5C6 LUCA
GeneTreeiENSGT00390000002866
HOVERGENiHBG080312
InParanoidiO75150
KOiK10696
OMAiNDQKEMK
OrthoDBiEOG091G01SA
PhylomeDBiO75150
TreeFamiTF323183

Enzyme and pathway databases

UniPathwayi
UPA00143

ReactomeiR-HSA-8866654 E3 ubiquitin ligases ubiquitinate target proteins
SIGNORiO75150

Miscellaneous databases

ChiTaRSiRNF40 human
GeneWikiiRNF40
GenomeRNAii9810
PROiPR:O75150
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000103549 Expressed in 179 organ(s), highest expression level in testis
CleanExiHS_RNF40
ExpressionAtlasiO75150 baseline and differential
GenevisibleiO75150 HS

Family and domain databases

Gene3Di3.30.40.10, 1 hit
InterProiView protein in InterPro
IPR013956 E3_ubiquit_lig_Bre1
IPR001841 Znf_RING
IPR013083 Znf_RING/FYVE/PHD
IPR017907 Znf_RING_CS
PANTHERiPTHR23163 PTHR23163, 1 hit
SMARTiView protein in SMART
SM00184 RING, 1 hit
PROSITEiView protein in PROSITE
PS00518 ZF_RING_1, 1 hit
PS50089 ZF_RING_2, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiBRE1B_HUMAN
AccessioniPrimary (citable) accession number: O75150
Secondary accession number(s): A0A024QZG0
, Q6AHZ6, Q6N005, Q7L3T6, Q8N615, Q96T18, Q9BSV9, Q9HC82
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: September 12, 2018
Last modified: November 7, 2018
This is version 163 of the entry and version 5 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Human chromosome 16
    Human chromosome 16: entries, gene names and cross-references to MIM
  3. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  4. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  5. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  6. PATHWAY comments
    Index of metabolic and biosynthesis pathways
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