UniProtKB - O75150 (BRE1B_HUMAN)
Protein
E3 ubiquitin-protein ligase BRE1B
Gene
RNF40
Organism
Homo sapiens (Human)
Status
Functioni
Component of the RNF20/40 E3 ubiquitin-protein ligase complex that mediates monoubiquitination of 'Lys-120' of histone H2B (H2BK120ub1). H2BK120ub1 gives a specific tag for epigenetic transcriptional activation and is also prerequisite for histone H3 'Lys-4' and 'Lys-79' methylation (H3K4me and H3K79me, respectively). It thereby plays a central role in histone code and gene regulation. The RNF20/40 complex forms a H2B ubiquitin ligase complex in cooperation with the E2 enzyme UBE2A or UBE2B; reports about the cooperation with UBE2E1/UBCH are contradictory. Required for transcriptional activation of Hox genes.2 Publications
Catalytic activityi
- S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.2 Publications EC:2.3.2.27
: protein ubiquitination Pathwayi
This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 948 – 987 | RING-typePROSITE-ProRule annotationAdd BLAST | 40 |
GO - Molecular functioni
- metal ion binding Source: UniProtKB-KW
- mRNA 3'-UTR binding Source: UniProtKB
- protein-containing complex binding Source: Ensembl
- protein homodimerization activity Source: UniProtKB
- syntaxin-1 binding Source: Ensembl
- ubiquitin conjugating enzyme binding Source: Ensembl
- ubiquitin protein ligase binding Source: UniProtKB
- ubiquitin-protein transferase activity Source: Ensembl
GO - Biological processi
- histone H2B ubiquitination Source: UniProtKB
- histone monoubiquitination Source: UniProtKB
- negative regulation of mRNA polyadenylation Source: UniProtKB
- positive regulation of histone H2B ubiquitination Source: UniProtKB
- positive regulation of proteasomal protein catabolic process Source: Ensembl
- positive regulation of protein polyubiquitination Source: Ensembl
- protein ubiquitination Source: Reactome
- response to peptide hormone Source: Ensembl
- ubiquitin-dependent protein catabolic process Source: UniProtKB
Keywordsi
Molecular function | Chromatin regulator, Transferase |
Biological process | Ubl conjugation pathway |
Ligand | Metal-binding, Zinc |
Enzyme and pathway databases
PathwayCommonsi | O75150 |
Reactomei | R-HSA-8866654, E3 ubiquitin ligases ubiquitinate target proteins |
SIGNORi | O75150 |
UniPathwayi | UPA00143 |
Names & Taxonomyi
Protein namesi | Recommended name: E3 ubiquitin-protein ligase BRE1B (EC:2.3.2.272 Publications)Short name: BRE1-B Alternative name(s): 95 kDa retinoblastoma-associated protein Short name: RBP95 RING finger protein 40 RING-type E3 ubiquitin transferase BRE1BCurated |
Gene namesi | Name:RNF40 Synonyms:BRE1B, KIAA0661 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:16867, RNF40 |
MIMi | 607700, gene |
neXtProti | NX_O75150 |
VEuPathDBi | HostDB:ENSG00000103549.21 |
Subcellular locationi
Nucleus
- Nucleus 1 Publication
Cytosol
- cytosol Source: Ensembl
Nucleus
- nucleoplasm Source: HPA
- nucleus Source: UniProtKB
Other locations
- axon terminus Source: Ensembl
- extrinsic component of membrane Source: Ensembl
- HULC complex Source: UniProtKB
- membrane Source: UniProtKB
- neuron projection Source: UniProtKB
- ubiquitin ligase complex Source: UniProtKB
Keywords - Cellular componenti
NucleusPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 109 | L → S: Abolishes interaction with RB1. 1 Publication | 1 | |
Mutagenesisi | 111 | C → M: Abolishes interaction with RB1. 1 Publication | 1 | |
Mutagenesisi | 113 | E → Q: Abolishes interaction with RB1. 1 Publication | 1 |
Organism-specific databases
DisGeNETi | 9810 |
OpenTargetsi | ENSG00000103549 |
PharmGKBi | PA34440 |
Miscellaneous databases
Pharosi | O75150, Tbio |
Genetic variation databases
BioMutai | RNF40 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000055839 | 1 – 1001 | E3 ubiquitin-protein ligase BRE1BAdd BLAST | 1001 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 20 | N6-acetyllysineBy similarity | 1 | |
Modified residuei | 42 | PhosphoserineBy similarity | 1 | |
Modified residuei | 355 | N6-acetyllysineBy similarity | 1 | |
Modified residuei | 517 | N6-acetyllysineBy similarity | 1 | |
Modified residuei | 528 | PhosphoserineBy similarity | 1 | |
Cross-linki | 578 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources | ||
Cross-linki | 579 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources | ||
Modified residuei | 585 | PhosphoserineBy similarity | 1 |
Keywords - PTMi
Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugationProteomic databases
EPDi | O75150 |
jPOSTi | O75150 |
MassIVEi | O75150 |
PaxDbi | O75150 |
PeptideAtlasi | O75150 |
PRIDEi | O75150 |
ProteomicsDBi | 49814 [O75150-1] 49815 [O75150-3] 49816 [O75150-4] |
PTM databases
iPTMneti | O75150 |
MetOSitei | O75150 |
PhosphoSitePlusi | O75150 |
Expressioni
Tissue specificityi
Ubiquitously expressed. Expressed at higher level in testis, heart and pancreas, while it is only weakly expressed in lung, skeletal muscle and small intestine.1 Publication
Gene expression databases
Bgeei | ENSG00000103549, Expressed in testis and 197 other tissues |
ExpressionAtlasi | O75150, baseline and differential |
Genevisiblei | O75150, HS |
Organism-specific databases
HPAi | ENSG00000103549, Low tissue specificity |
Interactioni
Subunit structurei
Component of the RNF20/40 complex (also known as BRE1 complex) probably composed of 2 copies of RNF20/BRE1A and 2 copies of RNF40/BRE1B.
Interacts with UBE2E1/UBCH6.
Interacts with RB1 and WAC.
4 PublicationsBinary interactionsi
Hide detailsO75150
GO - Molecular functioni
- protein homodimerization activity Source: UniProtKB
- syntaxin-1 binding Source: Ensembl
- ubiquitin conjugating enzyme binding Source: Ensembl
- ubiquitin protein ligase binding Source: UniProtKB
Protein-protein interaction databases
BioGRIDi | 115149, 116 interactors |
CORUMi | O75150 |
IntActi | O75150, 59 interactors |
MINTi | O75150 |
STRINGi | 9606.ENSP00000325677 |
Miscellaneous databases
RNActi | O75150, protein |
Family & Domainsi
Coiled coil
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Coiled coili | 44 – 91 | Sequence analysisAdd BLAST | 48 | |
Coiled coili | 189 – 377 | Sequence analysisAdd BLAST | 189 | |
Coiled coili | 437 – 525 | Sequence analysisAdd BLAST | 89 | |
Coiled coili | 627 – 946 | Sequence analysisAdd BLAST | 320 |
Sequence similaritiesi
Belongs to the BRE1 family.Curated
Zinc finger
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 948 – 987 | RING-typePROSITE-ProRule annotationAdd BLAST | 40 |
Keywords - Domaini
Coiled coil, Zinc-fingerPhylogenomic databases
eggNOGi | KOG0978, Eukaryota |
GeneTreei | ENSGT00390000002866 |
InParanoidi | O75150 |
OMAi | NDQKEMK |
OrthoDBi | 782448at2759 |
PhylomeDBi | O75150 |
TreeFami | TF323183 |
Family and domain databases
Gene3Di | 3.30.40.10, 1 hit |
InterProi | View protein in InterPro IPR013956, E3_ubiquit_lig_Bre1 IPR001841, Znf_RING IPR013083, Znf_RING/FYVE/PHD IPR017907, Znf_RING_CS |
PANTHERi | PTHR23163, PTHR23163, 1 hit |
SMARTi | View protein in SMART SM00184, RING, 1 hit |
PROSITEi | View protein in PROSITE PS00518, ZF_RING_1, 1 hit PS50089, ZF_RING_2, 1 hit |
s (3+)i Sequence
Sequence statusi: Complete.
This entry describes 3 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 3 described isoforms and 7 potential isoforms that are computationally mapped.Show allAlign All
Isoform 1 (identifier: O75150-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MSGPGNKRAA GDGGSGPPEK KLSREEKTTT TLIEPIRLGG ISSTEEMDLK
60 70 80 90 100
VLQFKNKKLA ERLEQRQACE DELRERIEKL EKRQATDDAT LLIVNRYWAQ
110 120 130 140 150
LDETVEALLR CHESQGELSS APEAPGTQEG PTCDGTPLPE PGTSELRDPL
160 170 180 190 200
LMQLRPPLSE PALAFVVALG ASSSEEVELE LQGRMEFSKA AVSRVVEASD
210 220 230 240 250
RLQRRVEELC QRVYSRGDSE PLSEAAQAHT RELGRENRRL QDLATQLQEK
260 270 280 290 300
HHRISLEYSE LQDKVTSAET KVLEMETTVE DLQWDIEKLR KREQKLNKHL
310 320 330 340 350
AEALEQLNSG YYVSGSSSGF QGGQITLSMQ KFEMLNAELE ENQELANSRM
360 370 380 390 400
AELEKLQAEL QGAVRTNERL KVALRSLPEE VVRETGEYRM LQAQFSLLYN
410 420 430 440 450
ESLQVKTQLD EARGLLLATK NSHLRHIEHM ESDELGLQKK LRTEVIQLED
460 470 480 490 500
TLAQVRKEYE MLRIEFEQNL AANEQAGPIN REMRHLISSL QNHNHQLKGD
510 520 530 540 550
AQRYKRKLRE VQAEIGKLRA QASGSAHSTP NLGHPEDSGV SAPAPGKEEG
560 570 580 590 600
GPGPVSTPDN RKEMAPVPGT TTTTTSVKKE ELVPSEEDFQ GITPGAQGPS
610 620 630 640 650
SRGREPEARP KRELREREGP SLGPPPVASA LSRADREKAK VEETKRKESE
660 670 680 690 700
LLKGLRAELK KAQESQKEMK LLLDMYKSAP KEQRDKVQLM AAERKAKAEV
710 720 730 740 750
DELRSRIREL EERDRRESKK IADEDALRRI RQAEEQIEHL QRKLGATKQE
760 770 780 790 800
EEALLSEMDV TGQAFEDMQE QNGRLLQQLR EKDDANFKLM SERIKANQIH
810 820 830 840 850
KLLREEKDEL GEQVLGLKSQ VDAQLLTVQK LEEKERALQG SLGGVEKELT
860 870 880 890 900
LRSQALELNK RKAVEAAQLA EDLKVQLEHV QTRLREIQPC LAESRAAREK
910 920 930 940 950
ESFNLKRAQE DISRLRRKLE KQRKVEVYAD ADEILQEEIK EYKARLTCPC
960 970 980 990 1000
CNTRKKDAVL TKCFHVFCFE CVRGRYEARQ RKCPKCNAAF GAHDFHRIYI
S
Computationally mapped potential isoform sequencesi
There are 7 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketH3BP71 | H3BP71_HUMAN | E3 ubiquitin protein ligase | RNF40 | 961 | Annotation score: | ||
H3BQ99 | H3BQ99_HUMAN | E3 ubiquitin protein ligase | RNF40 | 111 | Annotation score: | ||
H3BUL6 | H3BUL6_HUMAN | E3 ubiquitin protein ligase | RNF40 | 129 | Annotation score: | ||
I3L332 | I3L332_HUMAN | E3 ubiquitin protein ligase | RNF40 | 144 | Annotation score: | ||
H3BS50 | H3BS50_HUMAN | E3 ubiquitin protein ligase | RNF40 | 174 | Annotation score: | ||
A0A087WTK2 | A0A087WTK2_HUMAN | E3 ubiquitin protein ligase | RNF40 | 177 | Annotation score: | ||
R4GN66 | R4GN66_HUMAN | E3 ubiquitin-protein ligase BRE1B | RNF40 | 14 | Annotation score: |
Sequence cautioni
The sequence BAA31636 differs from that shown. Reason: Erroneous initiation. Extended N-terminus.Curated
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 56 | N → D in BAB55092 (PubMed:14702039).Curated | 1 | |
Sequence conflicti | 96 | R → L in AAG13723 (PubMed:10944455).Curated | 1 | |
Sequence conflicti | 108 | L → P in BAB55092 (PubMed:14702039).Curated | 1 | |
Sequence conflicti | 172 | S → G in BAB55092 (PubMed:14702039).Curated | 1 | |
Sequence conflicti | 387 | E → K in CAH10518 (PubMed:17974005).Curated | 1 | |
Sequence conflicti | 562 | K → E in CAE45869 (PubMed:17974005).Curated | 1 | |
Sequence conflicti | 600 | S → F in CAE45869 (PubMed:17974005).Curated | 1 | |
Sequence conflicti | 714 | D → N in BAB55092 (PubMed:14702039).Curated | 1 |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_055021 | 463 | R → H. Corresponds to variant dbSNP:rs11556801Ensembl. | 1 | |
Natural variantiVAR_055022 | 615 | R → Q. Corresponds to variant dbSNP:rs7195142Ensembl. | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_016681 | 332 – 431 | Missing in isoform 4. 1 PublicationAdd BLAST | 100 | |
Alternative sequenceiVSP_016682 | 822 – 838 | DAQLL…KERAL → WPPPACRLELERWGSWP in isoform 3. 1 PublicationAdd BLAST | 17 | |
Alternative sequenceiVSP_016683 | 839 – 1001 | Missing in isoform 3. 1 PublicationAdd BLAST | 163 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF122819 mRNA Translation: AAG13723.1 AB014561 mRNA Translation: BAA31636.2 Different initiation. AK027406 mRNA Translation: BAB55092.1 BX640763 mRNA Translation: CAE45869.1 Sequence problems. CR627431 mRNA Translation: CAH10518.1 AC106886 Genomic DNA No translation available. CH471192 Genomic DNA Translation: EAW52197.1 CH471192 Genomic DNA Translation: EAW52200.1 BC004527 mRNA Translation: AAH04527.2 BC006133 mRNA Translation: AAH06133.1 BC011769 mRNA Translation: AAH11769.1 BC018647 mRNA Translation: AAH18647.1 BC030802 mRNA Translation: AAH30802.2 |
CCDSi | CCDS10691.1 [O75150-1] CCDS55994.1 [O75150-4] |
PIRi | JC7363 |
RefSeqi | NP_001193962.1, NM_001207033.1 NP_001193963.1, NM_001207034.1 [O75150-4] NP_001273501.1, NM_001286572.2 [O75150-1] NP_055586.1, NM_014771.3 [O75150-1] |
Genome annotation databases
Ensembli | ENST00000324685; ENSP00000325677; ENSG00000103549 [O75150-1] ENST00000357890; ENSP00000350563; ENSG00000103549 [O75150-4] |
GeneIDi | 9810 |
KEGGi | hsa:9810 |
UCSCi | uc002dzq.4, human [O75150-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF122819 mRNA Translation: AAG13723.1 AB014561 mRNA Translation: BAA31636.2 Different initiation. AK027406 mRNA Translation: BAB55092.1 BX640763 mRNA Translation: CAE45869.1 Sequence problems. CR627431 mRNA Translation: CAH10518.1 AC106886 Genomic DNA No translation available. CH471192 Genomic DNA Translation: EAW52197.1 CH471192 Genomic DNA Translation: EAW52200.1 BC004527 mRNA Translation: AAH04527.2 BC006133 mRNA Translation: AAH06133.1 BC011769 mRNA Translation: AAH11769.1 BC018647 mRNA Translation: AAH18647.1 BC030802 mRNA Translation: AAH30802.2 |
CCDSi | CCDS10691.1 [O75150-1] CCDS55994.1 [O75150-4] |
PIRi | JC7363 |
RefSeqi | NP_001193962.1, NM_001207033.1 NP_001193963.1, NM_001207034.1 [O75150-4] NP_001273501.1, NM_001286572.2 [O75150-1] NP_055586.1, NM_014771.3 [O75150-1] |
3D structure databases
SMRi | O75150 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 115149, 116 interactors |
CORUMi | O75150 |
IntActi | O75150, 59 interactors |
MINTi | O75150 |
STRINGi | 9606.ENSP00000325677 |
PTM databases
iPTMneti | O75150 |
MetOSitei | O75150 |
PhosphoSitePlusi | O75150 |
Genetic variation databases
BioMutai | RNF40 |
Proteomic databases
EPDi | O75150 |
jPOSTi | O75150 |
MassIVEi | O75150 |
PaxDbi | O75150 |
PeptideAtlasi | O75150 |
PRIDEi | O75150 |
ProteomicsDBi | 49814 [O75150-1] 49815 [O75150-3] 49816 [O75150-4] |
Protocols and materials databases
Antibodypediai | 13809, 250 antibodies |
DNASUi | 9810 |
Genome annotation databases
Ensembli | ENST00000324685; ENSP00000325677; ENSG00000103549 [O75150-1] ENST00000357890; ENSP00000350563; ENSG00000103549 [O75150-4] |
GeneIDi | 9810 |
KEGGi | hsa:9810 |
UCSCi | uc002dzq.4, human [O75150-1] |
Organism-specific databases
CTDi | 9810 |
DisGeNETi | 9810 |
GeneCardsi | RNF40 |
HGNCi | HGNC:16867, RNF40 |
HPAi | ENSG00000103549, Low tissue specificity |
MIMi | 607700, gene |
neXtProti | NX_O75150 |
OpenTargetsi | ENSG00000103549 |
PharmGKBi | PA34440 |
VEuPathDBi | HostDB:ENSG00000103549.21 |
HUGEi | Search... |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG0978, Eukaryota |
GeneTreei | ENSGT00390000002866 |
InParanoidi | O75150 |
OMAi | NDQKEMK |
OrthoDBi | 782448at2759 |
PhylomeDBi | O75150 |
TreeFami | TF323183 |
Enzyme and pathway databases
UniPathwayi | UPA00143 |
PathwayCommonsi | O75150 |
Reactomei | R-HSA-8866654, E3 ubiquitin ligases ubiquitinate target proteins |
SIGNORi | O75150 |
Miscellaneous databases
BioGRID-ORCSi | 9810, 356 hits in 878 CRISPR screens |
ChiTaRSi | RNF40, human |
GeneWikii | RNF40 |
GenomeRNAii | 9810 |
Pharosi | O75150, Tbio |
PROi | PR:O75150 |
RNActi | O75150, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000103549, Expressed in testis and 197 other tissues |
ExpressionAtlasi | O75150, baseline and differential |
Genevisiblei | O75150, HS |
Family and domain databases
Gene3Di | 3.30.40.10, 1 hit |
InterProi | View protein in InterPro IPR013956, E3_ubiquit_lig_Bre1 IPR001841, Znf_RING IPR013083, Znf_RING/FYVE/PHD IPR017907, Znf_RING_CS |
PANTHERi | PTHR23163, PTHR23163, 1 hit |
SMARTi | View protein in SMART SM00184, RING, 1 hit |
PROSITEi | View protein in PROSITE PS00518, ZF_RING_1, 1 hit PS50089, ZF_RING_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | BRE1B_HUMAN | |
Accessioni | O75150Primary (citable) accession number: O75150 Secondary accession number(s): A0A024QZG0 Q9HC82 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | December 20, 2005 |
Last sequence update: | September 12, 2018 | |
Last modified: | February 10, 2021 | |
This is version 179 of the entry and version 5 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Human chromosome 16
Human chromosome 16: entries, gene names and cross-references to MIM - Human entries with genetic variants
List of human entries with genetic variants - Human variants curated from literature reports
Index of human variants curated from literature reports - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families