UniProtKB - O75106 (AOC2_HUMAN)
Retina-specific copper amine oxidase
AOC2
Functioni
Has a monoamine oxidase activity with substrate specificity for 2-phenylethylamine and tryptamine. May play a role in adipogenesis. May be a critical modulator of signal transmission in retina.
2 PublicationsCatalytic activityi
- EC:1.4.3.21By similarity
Cofactori
Protein has several cofactor binding sites:- Cu cationBy similarityNote: Binds 1 copper ion per subunit.By similarity
- Ca2+By similarityNote: Binds 2 calcium ions per subunit.By similarity
- L-topaquinoneBy similarityNote: Contains 1 topaquinone per subunit.By similarity
Kineticsi
- KM=0.056 mM for tryptamine1 Publication
- KM=0.077 mM for 2-phenylethylamine1 Publication
- KM=0.167 mM for benzylamine1 Publication
- KM=0.178 mM for p-tyramine1 Publication
- KM=1.7 mM for methylamine1 Publication
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 380 | Proton acceptorBy similarity | 1 | |
Active sitei | 465 | Schiff-base intermediate with substrate; via topaquinoneBy similarity | 1 | |
Metal bindingi | 516 | Copper; via tele nitrogenBy similarity | 1 | |
Metal bindingi | 518 | Copper; via tele nitrogenBy similarity | 1 | |
Metal bindingi | 525 | Calcium 1By similarity | 1 | |
Metal bindingi | 526 | Calcium 1; via carbonyl oxygenBy similarity | 1 | |
Metal bindingi | 527 | Calcium 1By similarity | 1 | |
Metal bindingi | 568 | Calcium 2By similarity | 1 | |
Metal bindingi | 659 | Calcium 2; via carbonyl oxygenBy similarity | 1 | |
Metal bindingi | 661 | Calcium 2By similarity | 1 | |
Metal bindingi | 663 | Calcium 2By similarity | 1 | |
Metal bindingi | 669 | Calcium 1By similarity | 1 | |
Metal bindingi | 670 | Calcium 1; via carbonyl oxygenBy similarity | 1 | |
Metal bindingi | 680 | Copper; via pros nitrogenBy similarity | 1 |
GO - Molecular functioni
- aliphatic-amine oxidase activity Source: UniProtKB-EC
- aminoacetone:oxygen oxidoreductase(deaminating) activity Source: UniProtKB-EC
- copper ion binding Source: GO_Central
- electron transfer activity Source: UniProtKB
- phenethylamine:oxygen oxidoreductase (deaminating) activity Source: UniProtKB-EC
- primary amine oxidase activity Source: GO_Central
- quinone binding Source: InterPro
- tryptamine:oxygen oxidoreductase (deaminating) activity Source: UniProtKB-EC
GO - Biological processi
- amine metabolic process Source: GO_Central
- catecholamine metabolic process Source: UniProtKB-KW
- visual perception Source: ProtInc
- xenobiotic metabolic process Source: Reactome
Keywordsi
Molecular function | Oxidoreductase |
Biological process | Catecholamine metabolism |
Ligand | Calcium, Copper, Metal-binding |
Enzyme and pathway databases
BRENDAi | 1.4.3.21, 2681 |
PathwayCommonsi | O75106 |
Reactomei | R-HSA-211945, Phase I - Functionalization of compounds |
SABIO-RKi | O75106 |
SignaLinki | O75106 |
Names & Taxonomyi
Protein namesi | Recommended name: Retina-specific copper amine oxidase (EC:1.4.3.21By similarity)Short name: RAO Alternative name(s): Amine oxidase [copper-containing] Semicarbazide-sensitive amine oxidase Short name: SSAO |
Gene namesi | Name:AOC2 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:549, AOC2 |
MIMi | 602268, gene |
neXtProti | NX_O75106 |
VEuPathDBi | HostDB:ENSG00000131480 |
Subcellular locationi
Plasma membrane
- Cell membrane 1 Publication; Peripheral membrane protein 1 Publication
Note: Present on the surface of the cells.1 Publication
Cytoplasm and Cytosol
- Cytoplasm 1 Publication
Note: Either not translocated to the plasma membrane or below detection level.1 Publication
Plasma Membrane
- plasma membrane Source: GO_Central
Other locations
- cytoplasm Source: UniProtKB-SubCell
Keywords - Cellular componenti
Cell membrane, Cytoplasm, MembranePathology & Biotechi
Organism-specific databases
DisGeNETi | 314 |
OpenTargetsi | ENSG00000131480 |
PharmGKBi | PA24839 |
Miscellaneous databases
Pharosi | O75106, Tchem |
Chemistry databases
ChEMBLi | CHEMBL4112 |
Genetic variation databases
BioMutai | AOC2 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 32 | Sequence analysisAdd BLAST | 32 | |
ChainiPRO_0000035671 | 33 – 756 | Retina-specific copper amine oxidaseAdd BLAST | 724 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Glycosylationi | 133 | N-linked (GlcNAc...) asparagineBy similarity | 1 | |
Glycosylationi | 198 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 226 | N-linked (GlcNAc...) asparagineBy similarity | 1 | |
Disulfide bondi | 398 ↔ 424 | By similarity | ||
Modified residuei | 465 | 2',4',5'-topaquinoneBy similarity | 1 | |
Glycosylationi | 588 | N-linked (GlcNAc...) (complex) asparagineBy similarity | 1 | |
Glycosylationi | 662 | N-linked (GlcNAc...) asparagineBy similarity | 1 | |
Disulfide bondi | 730 ↔ 737 | By similarity | ||
Disulfide bondi | 744 | InterchainBy similarity |
Post-translational modificationi
Keywords - PTMi
Disulfide bond, Glycoprotein, TPQProteomic databases
jPOSTi | O75106 |
MassIVEi | O75106 |
PaxDbi | O75106 |
PeptideAtlasi | O75106 |
PRIDEi | O75106 |
ProteomicsDBi | 49761 [O75106-1] 49762 [O75106-2] |
PTM databases
GlyConnecti | 1714, 2 N-Linked glycans (1 site) |
GlyGeni | O75106, 5 sites, 2 N-linked glycans (1 site) |
iPTMneti | O75106 |
PhosphoSitePlusi | O75106 |
Expressioni
Tissue specificityi
Inductioni
Gene expression databases
Bgeei | ENSG00000131480, Expressed in blood and 122 other tissues |
Genevisiblei | O75106, HS |
Organism-specific databases
HPAi | ENSG00000131480, Low tissue specificity |
Interactioni
Subunit structurei
Homodimer; disulfide-linked (By similarity).
Forms a heterodimer with AOC3, in vitro.
By similarity1 PublicationBinary interactionsi
Protein-protein interaction databases
BioGRIDi | 106811, 22 interactors |
IntActi | O75106, 5 interactors |
STRINGi | 9606.ENSP00000253799 |
Chemistry databases
BindingDBi | O75106 |
Miscellaneous databases
RNActi | O75106, protein |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 378 – 388 | Substrate bindingBy similarityAdd BLAST | 11 | |
Regioni | 462 – 467 | Substrate bindingBy similarity | 6 | |
Regioni | 574 – 581 | Heparin-bindingBy similarity | 8 |
Sequence similaritiesi
Keywords - Domaini
SignalPhylogenomic databases
eggNOGi | KOG1186, Eukaryota |
GeneTreei | ENSGT00950000183207 |
HOGENOMi | CLU_015739_1_0_1 |
InParanoidi | O75106 |
OMAi | QDFSKFF |
OrthoDBi | 1320015at2759 |
PhylomeDBi | O75106 |
TreeFami | TF314750 |
Family and domain databases
Gene3Di | 2.70.98.20, 1 hit |
InterProi | View protein in InterPro IPR032952, AOC2 IPR000269, Cu_amine_oxidase IPR015798, Cu_amine_oxidase_C IPR036460, Cu_amine_oxidase_C_sf IPR016182, Cu_amine_oxidase_N-reg IPR015800, Cu_amine_oxidase_N2 IPR015802, Cu_amine_oxidase_N3 |
PANTHERi | PTHR10638, PTHR10638, 1 hit PTHR10638:SF4, PTHR10638:SF4, 1 hit |
Pfami | View protein in Pfam PF01179, Cu_amine_oxid, 1 hit PF02727, Cu_amine_oxidN2, 1 hit PF02728, Cu_amine_oxidN3, 1 hit |
PRINTSi | PR00766, CUDAOXIDASE |
SUPFAMi | SSF49998, SSF49998, 1 hit SSF54416, SSF54416, 2 hits |
PROSITEi | View protein in PROSITE PS01164, COPPER_AMINE_OXID_1, 1 hit PS01165, COPPER_AMINE_OXID_2, 1 hit |
s (2)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MHLKIVLAFL ALSLITIFAL AYVLLTSPGG SSQPPHCPSV SHRAQPWPHP
60 70 80 90 100
GQSQLFADLS REELTAVMRF LTQRLGPGLV DAAQAQPSDN CIFSVELQLP
110 120 130 140 150
PKAAALAHLD RGSPPPAREA LAIVLFGGQP QPNVSELVVG PLPHPSYMRD
160 170 180 190 200
VTVERHGGPL PYHRRPVLRA EFTQMWRHLK EVELPKAPIF LSSTFNYNGS
210 220 230 240 250
TLAAVHATPR GLRSGDRATW MALYHNISGV GLFLHPVGLE LLLDHRALDP
260 270 280 290 300
AHWTVQQVFY LGHYYADLGQ LEREFKSGRL EVVRVPLPPP NGASSLRSRN
310 320 330 340 350
SPGPLPPLQF SPQGSQYSVQ GNLVVSSLWS FTFGHGVFSG LRIFDVRFQG
360 370 380 390 400
ERIAYEVSVQ ECVSIYGADS PKTMLTRYLD SSFGLGRNSR GLVRGVDCPY
410 420 430 440 450
QATMVDIHIL VGKGAVQLLP GAVCVFEEAQ GLPLRRHHNY LQNHFYGGLA
460 470 480 490 500
SSALVVRSVS SVGNYDYIWD FVLYPNGALE GRVHATGYIN TAFLKGGEEG
510 520 530 540 550
LLFGNRVGER VLGTVHTHAF HFKLDLDVAG LKNWVVAEDV VFKPVAAPWN
560 570 580 590 600
PEHWLQRPQL TRQVLGKEDL TAFSLGSPLP RYLYLASNQT NAWGHQRGYR
610 620 630 640 650
IQIHSPLGIH IPLESDMERA LSWGRYQLVV TQRKEEESQS SSIYHQNDIW
660 670 680 690 700
TPTVTFADFI NNETLLGEDL VAWVTASFLH IPHAEDIPNT VTLGNRVGFL
710 720 730 740 750
LRPYNFFDED PSIFSPGSVY FEKGQDAGLC SINPVACLPD LAACVPDLPP
FSYHGF
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 181 | E → D (PubMed:9119395).Curated | 1 | |
Sequence conflicti | 181 | E → D (PubMed:9722954).Curated | 1 | |
Sequence conflicti | 215 – 218 | GDRA → RERT (PubMed:9119395).Curated | 4 | |
Sequence conflicti | 215 – 218 | GDRA → RERT (PubMed:9722954).Curated | 4 | |
Sequence conflicti | 221 – 222 | MA → IG (PubMed:9119395).Curated | 2 | |
Sequence conflicti | 610 | H → Q (PubMed:9119395).Curated | 1 | |
Sequence conflicti | 610 | H → Q (PubMed:9722954).Curated | 1 |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_025022 | 5 | I → V1 PublicationCorresponds to variant dbSNP:rs34230945Ensembl. | 1 | |
Natural variantiVAR_025023 | 22 | Y → C1 PublicationCorresponds to variant dbSNP:rs34435306Ensembl. | 1 | |
Natural variantiVAR_025024 | 141 | P → L1 PublicationCorresponds to variant dbSNP:rs35833794EnsemblClinVar. | 1 | |
Natural variantiVAR_025025 | 273 | R → Q1 PublicationCorresponds to variant dbSNP:rs35508987Ensembl. | 1 | |
Natural variantiVAR_025026 | 427 | E → D1 PublicationCorresponds to variant dbSNP:rs34351794Ensembl. | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_006549 | 599 – 625 | Missing in isoform 2. 1 PublicationAdd BLAST | 27 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | D88213 mRNA Translation: BAA19001.1 AB012943 Genomic DNA Translation: BAA32590.1 AB012943 Genomic DNA Translation: BAA32589.1 AF081363 mRNA Translation: AAD39345.1 DQ060035 Genomic DNA Translation: AAY43129.1 AC016889 Genomic DNA No translation available. CH471152 Genomic DNA Translation: EAW60895.1 BC142641 mRNA Translation: AAI42642.1 |
CCDSi | CCDS11443.1 [O75106-1] CCDS45690.1 [O75106-2] |
RefSeqi | NP_001149.2, NM_001158.4 [O75106-2] NP_033720.2, NM_009590.3 [O75106-1] |
Genome annotation databases
Ensembli | ENST00000253799.8; ENSP00000253799.2; ENSG00000131480.9 ENST00000452774.2; ENSP00000406134.1; ENSG00000131480.9 [O75106-2] |
GeneIDi | 314 |
KEGGi | hsa:314 |
MANE-Selecti | ENST00000253799.8; ENSP00000253799.2; NM_009590.4; NP_033720.2 |
UCSCi | uc002ibt.5, human [O75106-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Web resourcesi
NIEHS-SNPs |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | D88213 mRNA Translation: BAA19001.1 AB012943 Genomic DNA Translation: BAA32590.1 AB012943 Genomic DNA Translation: BAA32589.1 AF081363 mRNA Translation: AAD39345.1 DQ060035 Genomic DNA Translation: AAY43129.1 AC016889 Genomic DNA No translation available. CH471152 Genomic DNA Translation: EAW60895.1 BC142641 mRNA Translation: AAI42642.1 |
CCDSi | CCDS11443.1 [O75106-1] CCDS45690.1 [O75106-2] |
RefSeqi | NP_001149.2, NM_001158.4 [O75106-2] NP_033720.2, NM_009590.3 [O75106-1] |
3D structure databases
AlphaFoldDBi | O75106 |
SMRi | O75106 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 106811, 22 interactors |
IntActi | O75106, 5 interactors |
STRINGi | 9606.ENSP00000253799 |
Chemistry databases
BindingDBi | O75106 |
ChEMBLi | CHEMBL4112 |
PTM databases
GlyConnecti | 1714, 2 N-Linked glycans (1 site) |
GlyGeni | O75106, 5 sites, 2 N-linked glycans (1 site) |
iPTMneti | O75106 |
PhosphoSitePlusi | O75106 |
Genetic variation databases
BioMutai | AOC2 |
Proteomic databases
jPOSTi | O75106 |
MassIVEi | O75106 |
PaxDbi | O75106 |
PeptideAtlasi | O75106 |
PRIDEi | O75106 |
ProteomicsDBi | 49761 [O75106-1] 49762 [O75106-2] |
Protocols and materials databases
Antibodypediai | 17156, 81 antibodies from 17 providers |
DNASUi | 314 |
Genome annotation databases
Ensembli | ENST00000253799.8; ENSP00000253799.2; ENSG00000131480.9 ENST00000452774.2; ENSP00000406134.1; ENSG00000131480.9 [O75106-2] |
GeneIDi | 314 |
KEGGi | hsa:314 |
MANE-Selecti | ENST00000253799.8; ENSP00000253799.2; NM_009590.4; NP_033720.2 |
UCSCi | uc002ibt.5, human [O75106-1] |
Organism-specific databases
CTDi | 314 |
DisGeNETi | 314 |
GeneCardsi | AOC2 |
HGNCi | HGNC:549, AOC2 |
HPAi | ENSG00000131480, Low tissue specificity |
MIMi | 602268, gene |
neXtProti | NX_O75106 |
OpenTargetsi | ENSG00000131480 |
PharmGKBi | PA24839 |
VEuPathDBi | HostDB:ENSG00000131480 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG1186, Eukaryota |
GeneTreei | ENSGT00950000183207 |
HOGENOMi | CLU_015739_1_0_1 |
InParanoidi | O75106 |
OMAi | QDFSKFF |
OrthoDBi | 1320015at2759 |
PhylomeDBi | O75106 |
TreeFami | TF314750 |
Enzyme and pathway databases
BRENDAi | 1.4.3.21, 2681 |
PathwayCommonsi | O75106 |
Reactomei | R-HSA-211945, Phase I - Functionalization of compounds |
SABIO-RKi | O75106 |
SignaLinki | O75106 |
Miscellaneous databases
BioGRID-ORCSi | 314, 13 hits in 1065 CRISPR screens |
GenomeRNAii | 314 |
Pharosi | O75106, Tchem |
PROi | PR:O75106 |
RNActi | O75106, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000131480, Expressed in blood and 122 other tissues |
Genevisiblei | O75106, HS |
Family and domain databases
Gene3Di | 2.70.98.20, 1 hit |
InterProi | View protein in InterPro IPR032952, AOC2 IPR000269, Cu_amine_oxidase IPR015798, Cu_amine_oxidase_C IPR036460, Cu_amine_oxidase_C_sf IPR016182, Cu_amine_oxidase_N-reg IPR015800, Cu_amine_oxidase_N2 IPR015802, Cu_amine_oxidase_N3 |
PANTHERi | PTHR10638, PTHR10638, 1 hit PTHR10638:SF4, PTHR10638:SF4, 1 hit |
Pfami | View protein in Pfam PF01179, Cu_amine_oxid, 1 hit PF02727, Cu_amine_oxidN2, 1 hit PF02728, Cu_amine_oxidN3, 1 hit |
PRINTSi | PR00766, CUDAOXIDASE |
SUPFAMi | SSF49998, SSF49998, 1 hit SSF54416, SSF54416, 2 hits |
PROSITEi | View protein in PROSITE PS01164, COPPER_AMINE_OXID_1, 1 hit PS01165, COPPER_AMINE_OXID_2, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | AOC2_HUMAN | |
Accessioni | O75106Primary (citable) accession number: O75106 Secondary accession number(s): A5PKW2 Q9UNY0 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 15, 1999 |
Last sequence update: | January 24, 2006 | |
Last modified: | May 25, 2022 | |
This is version 179 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Human chromosome 17
Human chromosome 17: entries, gene names and cross-references to MIM - Human entries with genetic variants
List of human entries with genetic variants - Human variants curated from literature reports
Index of human variants curated from literature reports - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families