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Entry version 128 (26 Feb 2020)
Sequence version 1 (01 Nov 1998)
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Protein

DNA primase large subunit

Gene

pri2

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score:

Annotation score:4 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
-Experimental evidence at protein leveli <p>This indicates the type of evidence that supports the existence of the protein. Note that the 'protein existence' evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

DNA primase is the polymerase that synthesizes small RNA primers for the Okazaki fragments made during discontinuous DNA replication.

<p>This subsection of the 'Function' section provides information relevant to cofactors. A cofactor is any non-protein substance required for a protein to be catalytically active. Some cofactors are inorganic, such as the metal atoms zinc, iron, and copper in various oxidation states. Others, such as most vitamins, are organic.<p><a href='/help/cofactor' target='_top'>More...</a></p>Cofactori

[4Fe-4S] clusterBy similarityNote: Binds 1 [4Fe-4S] cluster.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section indicates at which position the protein binds a given metal ion. The nature of the metal is indicated in the 'Description' field.<p><a href='/help/metal' target='_top'>More...</a></p>Metal bindingi291Iron-sulfur (4Fe-4S)By similarity1
Metal bindingi369Iron-sulfur (4Fe-4S)By similarity1
Metal bindingi386Iron-sulfur (4Fe-4S)By similarity1
Metal bindingi428Iron-sulfur (4Fe-4S)By similarity1

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

<p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

Molecular functionDNA-binding
Biological processDNA replication
Ligand4Fe-4S, Iron, Iron-sulfur, Metal-binding

Enzyme and pathway databases

Reactome - a knowledgebase of biological pathways and processes

More...
Reactomei
R-SPO-113501 Inhibition of replication initiation of damaged DNA by RB1/E2F1
R-SPO-174411 Polymerase switching on the C-strand of the telomere
R-SPO-174430 Telomere C-strand synthesis initiation
R-SPO-68952 DNA replication initiation
R-SPO-68962 Activation of the pre-replicative complex
R-SPO-69091 Polymerase switching
R-SPO-69166 Removal of the Flap Intermediate
R-SPO-69183 Processive synthesis on the lagging strand

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
Recommended name:
DNA primase large subunit
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: 'Name', 'Synonyms', 'Ordered locus names' and 'ORF names'.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
Name:pri2
Synonyms:spp2
ORF Names:SPBC17D11.06
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the 'taxonomic identifier' or 'taxid'.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri284812 [NCBI]
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section is present for entries that are part of a <a href="http://www.uniprot.org/proteomes">proteome</a>, i.e. of a set of proteins thought to be expressed by organisms whose genomes have been completely sequenced.<p><a href='/help/proteomes_manual' target='_top'>More...</a></p>Proteomesi
  • UP000002485 <p>A UniProt <a href="http://www.uniprot.org/manual/proteomes%5Fmanual">proteome</a> can consist of several components.<br></br>The component name refers to the genomic component encoding a set of proteins.<p><a href='/help/proteome_component' target='_top'>More...</a></p> Componenti: Chromosome II

Organism-specific databases

Eukaryotic Pathogen Database Resources

More...
EuPathDBi
FungiDB:SPBC17D11.06

Schizosaccharomyces pombe database

More...
PomBasei
SPBC17D11.06

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte & Seán O’Donoghue; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus, Primosome

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'PTM / Processing' section describes the extent of a polypeptide chain in the mature protein following processing.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_00000467751 – 459DNA primase large subunitAdd BLAST459

Proteomic databases

MaxQB - The MaxQuant DataBase

More...
MaxQBi
O74761

PaxDb, a database of protein abundance averages across all three domains of life

More...
PaxDbi
O74761

PRoteomics IDEntifications database

More...
PRIDEi
O74761

PTM databases

iPTMnet integrated resource for PTMs in systems biology context

More...
iPTMneti
O74761

SwissPalm database of S-palmitoylation events

More...
SwissPalmi
O74761

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

<p>This subsection of the <a href="http://www.uniprot.org/help/interaction%5Fsection">'Interaction'</a> section provides information about the protein quaternary structure and interaction(s) with other proteins or protein complexes (with the exception of physiological receptor-ligand interactions which are annotated in the <a href="http://www.uniprot.org/help/function%5Fsection">'Function'</a> section).<p><a href='/help/subunit_structure' target='_top'>More...</a></p>Subunit structurei

Heterodimer of a small subunit and a large subunit.

<p>This subsection of the '<a href="http://www.uniprot.org/help/interaction%5Fsection%27">Interaction</a> section provides information about binary protein-protein interactions. The data presented in this section are a quality-filtered subset of binary interactions automatically derived from the <a href="http://www.ebi.ac.uk/intact/">IntAct database</a>. It is updated on a monthly basis. Each binary interaction is displayed on a separate line.<p><a href='/help/binary_interactions' target='_top'>More...</a></p>Binary interactionsi

WithEntry#Exp.IntActNotes
O142152EBI-849075,EBI-849063

Protein-protein interaction databases

The Biological General Repository for Interaction Datasets (BioGrid)

More...
BioGridi
276477, 13 interactors

ComplexPortal: manually curated resource of macromolecular complexes

More...
ComplexPortali
CPX-2092 DNA polymerase alpha:primase complex

Protein interaction database and analysis system

More...
IntActi
O74761, 2 interactors

STRING: functional protein association networks

More...
STRINGi
4896.SPBC17D11.06.1

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

SWISS-MODEL Repository - a database of annotated 3D protein structure models

More...
SMRi
O74761

Database of comparative protein structure models

More...
ModBasei
Search...

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

<p>This subsection of the 'Family and domains' section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Phylogenomic databases

The HOGENOM Database of Homologous Genes from Fully Sequenced Organisms

More...
HOGENOMi
CLU_026253_1_0_1

InParanoid: Eukaryotic Ortholog Groups

More...
InParanoidi
O74761

KEGG Orthology (KO)

More...
KOi
K02685

Identification of Orthologs from Complete Genome Data

More...
OMAi
DCHGCPY

Database for complete collections of gene phylogenies

More...
PhylomeDBi
O74761

Family and domain databases

Conserved Domains Database

More...
CDDi
cd07322 PriL_PriS_Eukaryotic, 1 hit

Integrated resource of protein families, domains and functional sites

More...
InterProi
View protein in InterPro
IPR016558 DNA_primase_lsu_euk
IPR007238 DNA_primase_lsu_euk/arc

The PANTHER Classification System

More...
PANTHERi
PTHR10537 PTHR10537, 1 hit

Pfam protein domain database

More...
Pfami
View protein in Pfam
PF04104 DNA_primase_lrg, 1 hit

PIRSF; a whole-protein classification database

More...
PIRSFi
PIRSF009449 DNA_primase_large_subunit, 1 hit

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence%5Flength">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequencei

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences%5Fsection">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

O74761-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MFRTTKSRVV EKNQNFSSAS FESLSYPTRL NFYKKPPVSE ISIEEFETWA
60 70 80 90 100
IDRLVVLGEI ESAMLRNKTK NELNGIIKKI LDKRLPMSSN MARTVKGNSL
110 120 130 140 150
DEERRKDHYS HFILRLAFSR SDELRIRFLR AESTLFRFRF VNEETRERQA
160 170 180 190 200
FIDSLDFQWE SVSQEEKDVF YEKLQASSQR NIENESFFKV PFFKVPDLVE
210 220 230 240 250
RRAVFVHKGY AYVPFSEQVS LVVEEFEENL KKALESTAKS LPRLDEDDRL
260 270 280 290 300
LPILNHLSKG FVAPESSIVA PKSGAITAGQ VDSFVSHFPL CAKHLHEVLK
310 320 330 340 350
RDKHLRHFGR LQYGLFLKDI GLSVDEALVF WRKSFTNVTE DKFNKEYRYN
360 370 380 390 400
IRHTYGLEGN RKNYKGYNCQ QILTGPQLGP GDAHGCPYRT YSVNNLVSAL
410 420 430 440 450
ASMGIAPDSA GCREVVEAVK ARHYHIACTR VFELTHPNVG SLEESIHHPL

QYFQLSLES
Length:459
Mass (Da):53,053
Last modified:November 1, 1998 - v1
<p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:i811172102DDFC42E
GO

Sequence databases

Select the link destinations:

EMBL nucleotide sequence database

More...
EMBLi

GenBank nucleotide sequence database

More...
GenBanki

DNA Data Bank of Japan; a nucleotide sequence database

More...
DDBJi
Links Updated
CU329671 Genomic DNA Translation: CAA21077.1
AB027983 Genomic DNA Translation: BAA87287.1

Protein sequence database of the Protein Information Resource

More...
PIRi
T39717

NCBI Reference Sequences

More...
RefSeqi
NP_596380.1, NM_001022301.2

Genome annotation databases

Ensembl fungal genome annotation project

More...
EnsemblFungii
SPBC17D11.06.1; SPBC17D11.06.1:pep; SPBC17D11.06

Database of genes from NCBI RefSeq genomes

More...
GeneIDi
2539933

KEGG: Kyoto Encyclopedia of Genes and Genomes

More...
KEGGi
spo:SPBC17D11.06

<p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329671 Genomic DNA Translation: CAA21077.1
AB027983 Genomic DNA Translation: BAA87287.1
PIRiT39717
RefSeqiNP_596380.1, NM_001022301.2

3D structure databases

SMRiO74761
ModBaseiSearch...

Protein-protein interaction databases

BioGridi276477, 13 interactors
ComplexPortaliCPX-2092 DNA polymerase alpha:primase complex
IntActiO74761, 2 interactors
STRINGi4896.SPBC17D11.06.1

PTM databases

iPTMnetiO74761
SwissPalmiO74761

Proteomic databases

MaxQBiO74761
PaxDbiO74761
PRIDEiO74761

Genome annotation databases

EnsemblFungiiSPBC17D11.06.1; SPBC17D11.06.1:pep; SPBC17D11.06
GeneIDi2539933
KEGGispo:SPBC17D11.06

Organism-specific databases

EuPathDBiFungiDB:SPBC17D11.06
PomBaseiSPBC17D11.06

Phylogenomic databases

HOGENOMiCLU_026253_1_0_1
InParanoidiO74761
KOiK02685
OMAiDCHGCPY
PhylomeDBiO74761

Enzyme and pathway databases

ReactomeiR-SPO-113501 Inhibition of replication initiation of damaged DNA by RB1/E2F1
R-SPO-174411 Polymerase switching on the C-strand of the telomere
R-SPO-174430 Telomere C-strand synthesis initiation
R-SPO-68952 DNA replication initiation
R-SPO-68962 Activation of the pre-replicative complex
R-SPO-69091 Polymerase switching
R-SPO-69166 Removal of the Flap Intermediate
R-SPO-69183 Processive synthesis on the lagging strand

Miscellaneous databases

Protein Ontology

More...
PROi
PR:O74761

Family and domain databases

CDDicd07322 PriL_PriS_Eukaryotic, 1 hit
InterProiView protein in InterPro
IPR016558 DNA_primase_lsu_euk
IPR007238 DNA_primase_lsu_euk/arc
PANTHERiPTHR10537 PTHR10537, 1 hit
PfamiView protein in Pfam
PF04104 DNA_primase_lrg, 1 hit
PIRSFiPIRSF009449 DNA_primase_large_subunit, 1 hit

ProtoNet; Automatic hierarchical classification of proteins

More...
ProtoNeti
Search...

MobiDB: a database of protein disorder and mobility annotations

More...
MobiDBi
Search...

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This subsection of the 'Entry information' section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiPRI2_SCHPO
<p>This subsection of the 'Entry information' section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called 'Primary (citable) accession number'.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: O74761
Secondary accession number(s): Q9US83
<p>This subsection of the 'Entry information' section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification ('Last modified'). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 29, 2003
Last sequence update: November 1, 1998
Last modified: February 26, 2020
This is version 128 of the entry and version 1 of the sequence. See complete history.
<p>This subsection of the 'Entry information' section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

<p>This section contains any relevant information that doesn't fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
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