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Protein

DNA primase large subunit

Gene

pri2

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

DNA primase is the polymerase that synthesizes small RNA primers for the Okazaki fragments made during discontinuous DNA replication.

Cofactori

[4Fe-4S] clusterBy similarityNote: Binds 1 [4Fe-4S] cluster.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi291Iron-sulfur (4Fe-4S)By similarity1
Metal bindingi369Iron-sulfur (4Fe-4S)By similarity1
Metal bindingi386Iron-sulfur (4Fe-4S)By similarity1
Metal bindingi428Iron-sulfur (4Fe-4S)By similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDNA-binding, Nucleotidyltransferase, Transferase
Biological processDNA replication, Transcription
Ligand4Fe-4S, Iron, Iron-sulfur, Metal-binding

Enzyme and pathway databases

ReactomeiR-SPO-113501 Inhibition of replication initiation of damaged DNA by RB1/E2F1
R-SPO-174411 Polymerase switching on the C-strand of the telomere
R-SPO-174430 Telomere C-strand synthesis initiation
R-SPO-68952 DNA replication initiation
R-SPO-68962 Activation of the pre-replicative complex
R-SPO-69091 Polymerase switching
R-SPO-69166 Removal of the Flap Intermediate
R-SPO-69183 Processive synthesis on the lagging strand

Names & Taxonomyi

Protein namesi
Recommended name:
DNA primase large subunit (EC:2.7.7.-)
Gene namesi
Name:pri2
Synonyms:spp2
ORF Names:SPBC17D11.06
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome II

Organism-specific databases

EuPathDBiFungiDB:SPBC17D11.06
PomBaseiSPBC17D11.06

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

DNA-directed RNA polymerase, Nucleus, Primosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000467751 – 459DNA primase large subunitAdd BLAST459

Proteomic databases

MaxQBiO74761
PaxDbiO74761
PRIDEiO74761

PTM databases

iPTMnetiO74761
SwissPalmiO74761

Interactioni

Subunit structurei

Heterodimer of a small subunit and a large subunit.

Binary interactionsi

WithEntry#Exp.IntActNotes
pri1O142152EBI-849075,EBI-849063

Protein-protein interaction databases

BioGridi276477, 11 interactors
ComplexPortaliCPX-2092 DNA polymerase alpha:primase complex
IntActiO74761, 2 interactors
STRINGi4896.SPBC17D11.06.1

Structurei

3D structure databases

SMRiO74761
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

HOGENOMiHOG000212154
InParanoidiO74761
KOiK02685
OMAiDCHGCPY
OrthoDBiEOG092C27SX
PhylomeDBiO74761

Family and domain databases

CDDicd07322 PriL_PriS_Eukaryotic, 1 hit
InterProiView protein in InterPro
IPR016558 DNA_primase_lsu_euk
IPR007238 DNA_primase_lsu_euk/arc
PANTHERiPTHR10537 PTHR10537, 1 hit
PfamiView protein in Pfam
PF04104 DNA_primase_lrg, 1 hit
PIRSFiPIRSF009449 DNA_primase_large_subunit, 1 hit

Sequencei

Sequence statusi: Complete.

O74761-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFRTTKSRVV EKNQNFSSAS FESLSYPTRL NFYKKPPVSE ISIEEFETWA
60 70 80 90 100
IDRLVVLGEI ESAMLRNKTK NELNGIIKKI LDKRLPMSSN MARTVKGNSL
110 120 130 140 150
DEERRKDHYS HFILRLAFSR SDELRIRFLR AESTLFRFRF VNEETRERQA
160 170 180 190 200
FIDSLDFQWE SVSQEEKDVF YEKLQASSQR NIENESFFKV PFFKVPDLVE
210 220 230 240 250
RRAVFVHKGY AYVPFSEQVS LVVEEFEENL KKALESTAKS LPRLDEDDRL
260 270 280 290 300
LPILNHLSKG FVAPESSIVA PKSGAITAGQ VDSFVSHFPL CAKHLHEVLK
310 320 330 340 350
RDKHLRHFGR LQYGLFLKDI GLSVDEALVF WRKSFTNVTE DKFNKEYRYN
360 370 380 390 400
IRHTYGLEGN RKNYKGYNCQ QILTGPQLGP GDAHGCPYRT YSVNNLVSAL
410 420 430 440 450
ASMGIAPDSA GCREVVEAVK ARHYHIACTR VFELTHPNVG SLEESIHHPL

QYFQLSLES
Length:459
Mass (Da):53,053
Last modified:November 1, 1998 - v1
Checksum:i811172102DDFC42E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329671 Genomic DNA Translation: CAA21077.1
AB027983 Genomic DNA Translation: BAA87287.1
PIRiT39717
RefSeqiNP_596380.1, NM_001022301.2

Genome annotation databases

EnsemblFungiiSPBC17D11.06.1; SPBC17D11.06.1:pep; SPBC17D11.06
GeneIDi2539933
KEGGispo:SPBC17D11.06

Similar proteinsi

Entry informationi

Entry nameiPRI2_SCHPO
AccessioniPrimary (citable) accession number: O74761
Secondary accession number(s): Q9US83
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 29, 2003
Last sequence update: November 1, 1998
Last modified: June 20, 2018
This is version 120 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

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