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Entry version 150 (08 May 2019)
Sequence version 2 (30 May 2000)
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Protein

Eukaryotic peptide chain release factor GTP-binding subunit

Gene

sup35

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score:

Annotation score:5 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the ‘correct annotation’ for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
-Experimental evidence at protein leveli <p>This indicates the type of evidence that supports the existence of the protein. Note that the ‘protein existence’ evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Involved in translation termination. Stimulates the activity of ERF1. Binds guanine nucleotides.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the ‘Function’ section describes a region in the protein which binds nucleotide phosphates. It always involves more than one amino acid and includes all residues involved in nucleotide-binding.<p><a href='/help/np_bind' target='_top'>More...</a></p>Nucleotide bindingi245 – 252GTPBy similarity8
Nucleotide bindingi322 – 326GTPBy similarity5
Nucleotide bindingi384 – 387GTPBy similarity4

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

<p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

Biological processProtein biosynthesis
LigandGTP-binding, Nucleotide-binding

Enzyme and pathway databases

Reactome - a knowledgebase of biological pathways and processes

More...
Reactomei
R-SPO-72764 Eukaryotic Translation Termination
R-SPO-975956 Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC)
R-SPO-975957 Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC)

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
Recommended name:
Eukaryotic peptide chain release factor GTP-binding subunit
Alternative name(s):
ERF-3
Short name:
ERF3
ERF2
Polypeptide release factor 3
Translation release factor 3
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: ‘Name’, ‘Synonyms’, ‘Ordered locus names’ and ‘ORF names’.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
Name:sup35
ORF Names:SPCC584.04
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the ‘taxonomic identifier’ or ‘taxid’.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri284812 [NCBI]
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section is present for entries that are part of a <a href="http://www.uniprot.org/proteomes">proteome</a>, i.e. of a set of proteins thought to be expressed by organisms whose genomes have been completely sequenced.<p><a href='/help/proteomes_manual' target='_top'>More...</a></p>Proteomesi
  • UP000002485 <p>A UniProt <a href="http://www.uniprot.org/manual/proteomes_manual">proteome</a> can consist of several components. <br></br>The component name refers to the genomic component encoding a set of proteins.<p><a href='/help/proteome_component' target='_top'>More...</a></p> Componenti: Chromosome III

Organism-specific databases

Eukaryotic Pathogen Database Resources

More...
EuPathDBi
FungiDB:SPCC584.04

Schizosaccharomyces pombe database

More...
PomBasei
SPCC584.04 sup35

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte & Seán O’Donoghue; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the ‘PTM / Processing’ section describes the extent of a polypeptide chain in the mature protein following processing.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_00000914881 – 662Eukaryotic peptide chain release factor GTP-binding subunitAdd BLAST662

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the ‘PTM / Processing’ section specifies the position and type of each modified residue excluding <a href="http://www.uniprot.org/manual/lipid">lipids</a>, <a href="http://www.uniprot.org/manual/carbohyd">glycans</a> and <a href="http://www.uniprot.org/manual/crosslnk">protein cross-links</a>.<p><a href='/help/mod_res' target='_top'>More...</a></p>Modified residuei182Phosphothreonine1 Publication1
Modified residuei539Phosphoserine1 Publication1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQB - The MaxQuant DataBase

More...
MaxQBi
O74718

PaxDb, a database of protein abundance averages across all three domains of life

More...
PaxDbi
O74718

PRoteomics IDEntifications database

More...
PRIDEi
O74718

PTM databases

iPTMnet integrated resource for PTMs in systems biology context

More...
iPTMneti
O74718

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

GO - Molecular functioni

Protein-protein interaction databases

The Biological General Repository for Interaction Datasets (BioGrid)

More...
BioGridi
276042, 7 interactors

Database of interacting proteins

More...
DIPi
DIP-59469N

Protein interaction database and analysis system

More...
IntActi
O74718, 1 interactor

STRING: functional protein association networks

More...
STRINGi
4896.SPCC584.04.1

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

Secondary structure

1662
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

Select the link destinations:

Protein Data Bank Europe

More...
PDBei

Protein Data Bank RCSB

More...
RCSB PDBi

Protein Data Bank Japan

More...
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1R5BX-ray2.35A196-662[»]
1R5NX-ray2.90A196-662[»]
1R5OX-ray3.20A196-662[»]
3E20X-ray3.50A/D/E/J467-662[»]

Database of protein disorder

More...
DisProti
DP00396

SWISS-MODEL Repository - a database of annotated 3D protein structure models

More...
SMRi
O74718

Database of comparative protein structure models

More...
ModBasei
Search...

MobiDB: a database of protein disorder and mobility annotations

More...
MobiDBi
Search...

Miscellaneous databases

Relative evolutionary importance of amino acids within a protein sequence

More...
EvolutionaryTracei
O74718

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/family_and_domains_section">Family and Domains</a> section describes the position and type of a domain, which is defined as a specific combination of secondary structures organized into a characteristic three-dimensional structure or fold.<p><a href='/help/domain' target='_top'>More...</a></p>Domaini236 – 464tr-type GPROSITE-ProRule annotationAdd BLAST229

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the ‘Family and Domains’ section describes a region of interest that cannot be described in other subsections.<p><a href='/help/region' target='_top'>More...</a></p>Regioni245 – 252G1PROSITE-ProRule annotation8
Regioni301 – 305G2PROSITE-ProRule annotation5
Regioni322 – 325G3PROSITE-ProRule annotation4
Regioni384 – 387G4PROSITE-ProRule annotation4
Regioni427 – 429G5PROSITE-ProRule annotation3

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Keywords - Domaini

Repeat

Phylogenomic databases

The HOGENOM Database of Homologous Genes from Fully Sequenced Organisms

More...
HOGENOMi
HOG000229291

InParanoid: Eukaryotic Ortholog Groups

More...
InParanoidi
O74718

KEGG Orthology (KO)

More...
KOi
K03267

Identification of Orthologs from Complete Genome Data

More...
OMAi
NSAVTME

Database for complete collections of gene phylogenies

More...
PhylomeDBi
O74718

Family and domain databases

Integrated resource of protein families, domains and functional sites

More...
InterProi
View protein in InterPro
IPR031157 G_TR_CS
IPR027417 P-loop_NTPase
IPR003285 Sup35
IPR000795 TF_GTP-bd_dom
IPR009000 Transl_B-barrel_sf
IPR009001 Transl_elong_EF1A/Init_IF2_C
IPR004160 Transl_elong_EFTu/EF1A_C

Pfam protein domain database

More...
Pfami
View protein in Pfam
PF00009 GTP_EFTU, 1 hit
PF03143 GTP_EFTU_D3, 1 hit

Protein Motif fingerprint database; a protein domain database

More...
PRINTSi
PR00315 ELONGATNFCT
PR01343 YEASTERF

Superfamily database of structural and functional annotation

More...
SUPFAMi
SSF50447 SSF50447, 1 hit
SSF50465 SSF50465, 1 hit
SSF52540 SSF52540, 1 hit

PROSITE; a protein domain and family database

More...
PROSITEi
View protein in PROSITE
PS00301 G_TR_1, 1 hit
PS51722 G_TR_2, 1 hit

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence_length">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>.<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequencei

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences_section">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

O74718-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MASNQPNNGE QDEQLAKQTS KLSMSAKAPT FTPKAAPFIP SFQRPGFVPV
60 70 80 90 100
NNIAGGYPYA QYTGQGQNSN SPHPTKSYQQ YYQKPTGNTV DEDKSRVPDF
110 120 130 140 150
SKKKSFVPPK PAIPKGKVLS LGGNTSAPKS TKPISISLGG TKAPTTTKPA
160 170 180 190 200
APAAQSKTET PAPKVTSEST KKETAAPPPQ ETPTKSADAE LAKTPSAPAA
210 220 230 240 250
ALKKAAEAAE PATVTEDATD LQNEVDQELL KDMYGKEHVN IVFIGHVDAG
260 270 280 290 300
KSTLGGNILF LTGMVDKRTM EKIEREAKEA GKESWYLSWA LDSTSEEREK
310 320 330 340 350
GKTVEVGRAY FETEHRRFSL LDAPGHKGYV TNMINGASQA DIGVLVISAR
360 370 380 390 400
RGEFEAGFER GGQTREHAVL ARTQGINHLV VVINKMDEPS VQWSEERYKE
410 420 430 440 450
CVDKLSMFLR RVAGYNSKTD VKYMPVSAYT GQNVKDRVDS SVCPWYQGPS
460 470 480 490 500
LLEYLDSMTH LERKVNAPFI MPIASKYKDL GTILEGKIEA GSIKKNSNVL
510 520 530 540 550
VMPINQTLEV TAIYDEADEE ISSSICGDQV RLRVRGDDSD VQTGYVLTST
560 570 580 590 600
KNPVHATTRF IAQIAILELP SILTTGYSCV MHIHTAVEEV SFAKLLHKLD
610 620 630 640 650
KTNRKSKKPP MFATKGMKII AELETQTPVC MERFEDYQYM GRFTLRDQGT
660
TVAVGKVVKI LD
Length:662
Mass (Da):72,556
Last modified:May 30, 2000 - v2
<p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:i54AE41EA6AF6168A
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the ‘Sequence’ section reports difference(s) between the canonical sequence (displayed by default in the entry) and the different sequence submissions merged in the entry. These various submissions may originate from different sequencing projects, different types of experiments, or different biological samples. Sequence conflicts are usually of unknown origin.<p><a href='/help/conflict' target='_top'>More...</a></p>Sequence conflicti79 – 80QQ → LL in BAA33530 (PubMed:9701287).Curated2
Sequence conflicti388E → D in BAA13868 (PubMed:9501991).Curated1
Sequence conflicti397 – 398RY → SN in BAA13868 (PubMed:9501991).Curated2
Sequence conflicti589E → G in BAA33530 (PubMed:9701287).Curated1

Sequence databases

Select the link destinations:

EMBL nucleotide sequence database

More...
EMBLi

GenBank nucleotide sequence database

More...
GenBanki

DNA Data Bank of Japan; a nucleotide sequence database

More...
DDBJi
Links Updated
D79214 Genomic DNA Translation: BAA33530.1
CU329672 Genomic DNA Translation: CAA21821.1
D89207 mRNA Translation: BAA13868.1

Protein sequence database of the Protein Information Resource

More...
PIRi
T41442
T43011
T51948

NCBI Reference Sequences

More...
RefSeqi
NP_588225.1, NM_001023215.2

Genome annotation databases

Ensembl fungal genome annotation project

More...
EnsemblFungii
SPCC584.04.1; SPCC584.04.1:pep; SPCC584.04

Database of genes from NCBI RefSeq genomes

More...
GeneIDi
2539479

KEGG: Kyoto Encyclopedia of Genes and Genomes

More...
KEGGi
spo:SPCC584.04

<p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D79214 Genomic DNA Translation: BAA33530.1
CU329672 Genomic DNA Translation: CAA21821.1
D89207 mRNA Translation: BAA13868.1
PIRiT41442
T43011
T51948
RefSeqiNP_588225.1, NM_001023215.2

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1R5BX-ray2.35A196-662[»]
1R5NX-ray2.90A196-662[»]
1R5OX-ray3.20A196-662[»]
3E20X-ray3.50A/D/E/J467-662[»]
DisProtiDP00396
SMRiO74718
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi276042, 7 interactors
DIPiDIP-59469N
IntActiO74718, 1 interactor
STRINGi4896.SPCC584.04.1

PTM databases

iPTMnetiO74718

Proteomic databases

MaxQBiO74718
PaxDbiO74718
PRIDEiO74718

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiSPCC584.04.1; SPCC584.04.1:pep; SPCC584.04
GeneIDi2539479
KEGGispo:SPCC584.04

Organism-specific databases

EuPathDBiFungiDB:SPCC584.04
PomBaseiSPCC584.04 sup35

Phylogenomic databases

HOGENOMiHOG000229291
InParanoidiO74718
KOiK03267
OMAiNSAVTME
PhylomeDBiO74718

Enzyme and pathway databases

ReactomeiR-SPO-72764 Eukaryotic Translation Termination
R-SPO-975956 Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC)
R-SPO-975957 Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC)

Miscellaneous databases

EvolutionaryTraceiO74718

Protein Ontology

More...
PROi
PR:O74718

Family and domain databases

InterProiView protein in InterPro
IPR031157 G_TR_CS
IPR027417 P-loop_NTPase
IPR003285 Sup35
IPR000795 TF_GTP-bd_dom
IPR009000 Transl_B-barrel_sf
IPR009001 Transl_elong_EF1A/Init_IF2_C
IPR004160 Transl_elong_EFTu/EF1A_C
PfamiView protein in Pfam
PF00009 GTP_EFTU, 1 hit
PF03143 GTP_EFTU_D3, 1 hit
PRINTSiPR00315 ELONGATNFCT
PR01343 YEASTERF
SUPFAMiSSF50447 SSF50447, 1 hit
SSF50465 SSF50465, 1 hit
SSF52540 SSF52540, 1 hit
PROSITEiView protein in PROSITE
PS00301 G_TR_1, 1 hit
PS51722 G_TR_2, 1 hit

ProtoNet; Automatic hierarchical classification of proteins

More...
ProtoNeti
Search...

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This subsection of the ‘Entry information’ section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiERF3_SCHPO
<p>This subsection of the ‘Entry information’ section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called ‘Primary (citable) accession number’.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: O74718
Secondary accession number(s): P78857
<p>This subsection of the ‘Entry information’ section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification (‘Last modified’). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: May 30, 2000
Last modified: May 8, 2019
This is version 150 of the entry and version 2 of the sequence. See complete history.
<p>This subsection of the ‘Entry information’ section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
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