UniProtKB - O74237 (XYL1_CANTE)
Protein
NAD(P)H-dependent D-xylose reductase
Gene
XYL1
Organism
Candida tenuis (Yeast) (Yamadazyma tenuis)
Status
Functioni
Reduces D-xylose into xylitol. Has a preference for NADPH, but can also utilize NADH as cosubstrate.
Catalytic activityi
Kineticsi
- KM=142 mM for xylose2 Publications
- KM=38 µM for NADH2 Publications
- KM=3 µM for NADPH2 Publications
: D-xylose degradation Pathwayi
This protein is involved in the pathway D-xylose degradation, which is part of Carbohydrate metabolism.View all proteins of this organism that are known to be involved in the pathway D-xylose degradation and in Carbohydrate metabolism.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 52 | Proton donor | 1 | |
Sitei | 81 | Lowers pKa of active site TyrBy similarity | 1 | |
Binding sitei | 114 | SubstrateBy similarity | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 169 – 170 | NAD or NADP3 Publications | 2 | |
Nucleotide bindingi | 218 – 227 | NAD or NADP3 Publications | 10 | |
Nucleotide bindingi | 274 – 284 | NAD or NADP3 PublicationsAdd BLAST | 11 |
GO - Molecular functioni
- oxidoreductase activity Source: UniProtKB-KW
GO - Biological processi
- D-xylose catabolic process Source: UniProtKB-UniPathway
Keywordsi
Molecular function | Oxidoreductase |
Biological process | Carbohydrate metabolism, Xylose metabolism |
Ligand | NAD, NADP |
Enzyme and pathway databases
BRENDAi | 1.1.1.307, 1144 |
SABIO-RKi | O74237 |
UniPathwayi | UPA00810 |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:XYL1 Synonyms:XYLR |
Organismi | Candida tenuis (Yeast) (Yamadazyma tenuis) |
Taxonomic identifieri | 2315449 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Debaryomycetaceae › Yamadazyma |
Pathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 24 | W → F or Y: Strongly reduced affinity for xylose. Reduces NADH-dependent enzyme activity by over 96%. 1 Publication | 1 | |
Mutagenesisi | 51 | D → A: Slightly reduced enzyme activity. 1 Publication | 1 | |
Mutagenesisi | 274 | K → E: Reduces enzyme activity about 1000-fold. 1 Publication | 1 | |
Mutagenesisi | 274 | K → G or M: Reduces affinity for NAD and NADP. 1 Publication | 1 | |
Mutagenesisi | 274 | K → R: Increases affinity for NAD. Strongly reduced enzyme activity with NADP; when associated with D-276. 1 Publication | 1 | |
Mutagenesisi | 275 | S → A: Decreases affinity for NAD and NADP. 1 Publication | 1 | |
Mutagenesisi | 276 | N → D: Increases affinity for NAD. Decreases affinity for NADP. Strongly reduced enzyme activity with NADP; when associated with R-274. 1 Publication | 1 | |
Mutagenesisi | 280 | R → H: Increases affinity for NAD. 1 Publication | 1 | |
Mutagenesisi | 310 | N → A or D: Strongly decreased affinity for xylose. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000124661 | 1 – 322 | NAD(P)H-dependent D-xylose reductaseAdd BLAST | 322 |
Interactioni
Subunit structurei
Homodimer.
4 PublicationsStructurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | O74237 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | O74237 |
Family & Domainsi
Sequence similaritiesi
Belongs to the aldo/keto reductase family.Curated
Family and domain databases
CDDi | cd06660, Aldo_ket_red, 1 hit |
Gene3Di | 3.20.20.100, 1 hit |
InterProi | View protein in InterPro IPR018170, Aldo/ket_reductase_CS IPR020471, Aldo/keto_reductase IPR023210, NADP_OxRdtase_dom IPR036812, NADP_OxRdtase_dom_sf |
PANTHERi | PTHR11732, PTHR11732, 1 hit |
Pfami | View protein in Pfam PF00248, Aldo_ket_red, 1 hit |
PIRSFi | PIRSF000097, AKR, 1 hit |
PRINTSi | PR00069, ALDKETRDTASE |
SUPFAMi | SSF51430, SSF51430, 1 hit |
PROSITEi | View protein in PROSITE PS00798, ALDOKETO_REDUCTASE_1, 1 hit PS00062, ALDOKETO_REDUCTASE_2, 1 hit PS00063, ALDOKETO_REDUCTASE_3, 1 hit |
i Sequence
Sequence statusi: Complete.
O74237-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSASIPDIKL SSGHLMPSIG FGCWKLANAT AGEQVYQAIK AGYRLFDGAE
60 70 80 90 100
DYGNEKEVGD GVKRAIDEGL VKREEIFLTS KLWNNYHDPK NVETALNKTL
110 120 130 140 150
ADLKVDYVDL FLIHFPIAFK FVPIEEKYPP GFYCGDGNNF VYEDVPILET
160 170 180 190 200
WKALEKLVAA GKIKSIGVSN FPGALLLDLL RGATIKPAVL QVEHHPYLQQ
210 220 230 240 250
PKLIEFAQKA GVTITAYSSF GPQSFVEMNQ GRALNTPTLF AHDTIKAIAA
260 270 280 290 300
KYNKTPAEVL LRWAAQRGIA VIPKSNLPER LVQNRSFNTF DLTKEDFEEI
310 320
AKLDIGLRFN DPWDWDNIPI FV
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF074484 Genomic DNA Translation: AAC25601.1 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF074484 Genomic DNA Translation: AAC25601.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1JEZ | X-ray | 2.20 | A/B | 1-322 | [»] | |
1K8C | X-ray | 2.10 | A/B/C/D | 1-322 | [»] | |
1MI3 | X-ray | 1.80 | A/B/C/D | 1-322 | [»] | |
1R38 | X-ray | 2.20 | A/B/C/D | 1-322 | [»] | |
1SM9 | X-ray | 2.20 | A/B/C/D | 1-322 | [»] | |
1YE4 | X-ray | 2.40 | A/B/C/D | 1-322 | [»] | |
1YE6 | X-ray | 2.30 | A/B/C/D | 1-322 | [»] | |
1Z9A | X-ray | 2.40 | A/B/C/D | 2-322 | [»] | |
SMRi | O74237 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Enzyme and pathway databases
UniPathwayi | UPA00810 |
BRENDAi | 1.1.1.307, 1144 |
SABIO-RKi | O74237 |
Miscellaneous databases
EvolutionaryTracei | O74237 |
Family and domain databases
CDDi | cd06660, Aldo_ket_red, 1 hit |
Gene3Di | 3.20.20.100, 1 hit |
InterProi | View protein in InterPro IPR018170, Aldo/ket_reductase_CS IPR020471, Aldo/keto_reductase IPR023210, NADP_OxRdtase_dom IPR036812, NADP_OxRdtase_dom_sf |
PANTHERi | PTHR11732, PTHR11732, 1 hit |
Pfami | View protein in Pfam PF00248, Aldo_ket_red, 1 hit |
PIRSFi | PIRSF000097, AKR, 1 hit |
PRINTSi | PR00069, ALDKETRDTASE |
SUPFAMi | SSF51430, SSF51430, 1 hit |
PROSITEi | View protein in PROSITE PS00798, ALDOKETO_REDUCTASE_1, 1 hit PS00062, ALDOKETO_REDUCTASE_2, 1 hit PS00063, ALDOKETO_REDUCTASE_3, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | XYL1_CANTE | |
Accessioni | O74237Primary (citable) accession number: O74237 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 11, 2004 |
Last sequence update: | November 1, 1998 | |
Last modified: | October 7, 2020 | |
This is version 83 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structureDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families