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Protein

Methylated-DNA--protein-cysteine methyltransferase

Gene

ogt

Organism
Thermococcus kodakarensis (strain ATCC BAA-918 / JCM 12380 / KOD1) (Pyrococcus kodakaraensis (strain KOD1))
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) and O4-methylthymine (O4-MeT) in DNA. Repairs the methylated nucleobase in DNA by stoichiometrically transferring the methyl group to a cysteine residue in the enzyme. This is a suicide reaction: the enzyme is irreversibly inactivated.UniRule annotation1 Publication

Miscellaneous

This enzyme catalyzes only one turnover and therefore is not strictly catalytic. According to one definition, an enzyme is a biocatalyst that acts repeatedly and over many reaction cycles.Curated

Catalytic activityi

DNA (containing 6-O-methylguanine) + protein L-cysteine = DNA (without 6-O-methylguanine) + protein S-methyl-L-cysteine.UniRule annotation1 Publication
DNA (containing 4-O-methylthymine) + protein L-cysteine = DNA (without 4-O-methylthymine) + protein S-methyl-L-cysteine.UniRule annotation1 Publication

Temperature dependencei

Activity is stable at 90 degrees Celsius for at least 30 minutes.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei141Nucleophile; methyl group acceptorUniRule annotation1 Publication1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionMethyltransferase, Transferase
Biological processDNA damage, DNA repair

Enzyme and pathway databases

BioCyciTKOD69014:G1G2A-1986-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Methylated-DNA--protein-cysteine methyltransferaseUniRule annotation (EC:2.1.1.63UniRule annotation1 Publication)
Alternative name(s):
6-O-methylguanine-DNA methyltransferaseUniRule annotation
Short name:
MGMT1 PublicationUniRule annotation
O-6-methylguanine-DNA-alkyltransferaseUniRule annotation
Pk-MGMT1 Publication
Gene namesi
Name:ogtUniRule annotation
Synonyms:mgtk1 Publication
Ordered Locus Names:TK1971
OrganismiThermococcus kodakarensis (strain ATCC BAA-918 / JCM 12380 / KOD1) (Pyrococcus kodakaraensis (strain KOD1))
Taxonomic identifieri69014 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaeThermococcus
Proteomesi
  • UP000000536 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001393851 – 174Methylated-DNA--protein-cysteine methyltransferaseAdd BLAST174

Interactioni

Protein-protein interaction databases

STRINGi69014.TK1971

Structurei

Secondary structure

1174
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliO74023
SMRiO74023
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO74023

Family & Domainsi

Sequence similaritiesi

Belongs to the MGMT family.UniRule annotationCurated

Phylogenomic databases

eggNOGiarCOG02724 Archaea
COG0350 LUCA
HOGENOMiHOG000244137
InParanoidiO74023
KOiK00567
OMAiKRNPYPI
OrthoDBiPOG093Z0E1E

Family and domain databases

CDDicd06445 ATase, 1 hit
Gene3Di1.10.10.10, 1 hit
HAMAPiMF_00772 OGT, 1 hit
InterProiView protein in InterPro
IPR015236 DUF1938
IPR001497 MethylDNA_cys_MeTrfase_AS
IPR014048 MethylDNA_cys_MeTrfase_DNA-bd
IPR036217 MethylDNA_cys_MeTrfase_DNAb
IPR023546 MGMT
IPR036631 MGMT_N_sf
IPR036388 WH-like_DNA-bd_sf
PfamiView protein in Pfam
PF01035 DNA_binding_1, 1 hit
PF09153 DUF1938, 1 hit
ProDomiView protein in ProDom or Entries sharing at least one domain
PD068053 DUF1938, 1 hit
SUPFAMiSSF46767 SSF46767, 1 hit
SSF53155 SSF53155, 1 hit
TIGRFAMsiTIGR00589 ogt, 1 hit
PROSITEiView protein in PROSITE
PS00374 MGMT, 1 hit

Sequencei

Sequence statusi: Complete.

O74023-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MLSVEKFRVG ERVVWIGVIF SGRVQGIAFA FDRGTLMKRI HDLAEHLGKR
60 70 80 90 100
GVSISLDVQP SDYPEKVFKV LIGELDNASF LRELSFEGVT PFEKKVYEWL
110 120 130 140 150
TKNVKRGSVI TYGDLAKALN TSPRAVGGAM KRNPYPIVVP CHRVVAHDGI
160 170
GYYSSGIEEK KFLLEIEGVK EWTS
Length:174
Mass (Da):19,517
Last modified:November 1, 1998 - v1
Checksum:i1AD95478CE2EF72F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D86335 Genomic DNA Translation: BAA29044.1
AP006878 Genomic DNA Translation: BAD86160.1
PIRiT44680
RefSeqiWP_011250921.1, NC_006624.1

Genome annotation databases

EnsemblBacteriaiBAD86160; BAD86160; TK1971
GeneIDi3234736
KEGGitko:TK1971
PATRICifig|69014.16.peg.1925

Similar proteinsi

Entry informationi

Entry nameiOGT_THEKO
AccessioniPrimary (citable) accession number: O74023
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 10, 2004
Last sequence update: November 1, 1998
Last modified: February 28, 2018
This is version 111 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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